Standard name
Human Ortholog
Description Zinc finger transcription factor involved in multidrug resistance; Zn(2)-Cys(6) zinc finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.07 0.09 0.11 0.1 0.06 – 0.08 0.06 0.07 0.08 0.11 0.14 0.16 0.22 0.24 0.23 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0.06 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.11 0.05 0.11 0.14 0.36 0.43 – 0.58 0.67 0.7 0.57 0.16 0.07 0.05 0.05 0 0 0 0 0 0 0 0
Nucleus 0.89 0.84 0.79 0.78 0.69 0.63 0.67 – 0.58 0.51 0.47 0.55 0.81 0.74 0.71 0.55 0.44 0.56 0.8 0.71 0.67 0.41 0.39 0.36
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0.05 0 0 0.11 0.11 0.05
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.07 0.11 0.05 0.07 – 0.05 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0.05 0.06 0
Vac/Vac Membrane 0 0.06 0.05 0 0 0 0 – 0 0 0 0 0.05 0 0.07 0.2 0.28 0.2 0.06 0.13 0.14 0.21 0.25 0.36
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0
Bud 0 1 0 0 0 6 11 – 9 6 9 9 1 0 2 1 0 0 2 6 2 4 3 1
Bud Neck 0 0 1 1 3 4 3 – 9 6 2 1 0 0 0 0 1 0 0 1 1 2 2 1
Bud Site 1 0 1 2 1 7 10 – 15 15 11 14 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0
Cytoplasm 11 31 17 18 24 36 23 – 28 22 17 25 23 22 26 83 60 52 1 9 3 2 2 2
Endoplasmic Reticulum 0 1 1 0 2 0 0 – 0 0 0 0 0 0 1 24 8 17 0 0 1 0 1 4
Endosome 3 7 8 7 6 7 6 – 2 0 2 6 0 4 1 14 17 9 7 15 6 8 7 6
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 2 0 0 1 0 1 0 0
Mitochondria 26 61 13 23 31 127 162 – 194 237 171 186 35 11 8 19 8 9 2 7 7 5 6 4
Nucleus 283 482 189 165 158 223 252 – 197 181 116 179 176 118 114 204 113 127 282 475 201 58 76 54
Nuclear Periphery 0 1 0 1 2 1 0 – 2 0 0 1 0 0 1 0 0 0 0 0 1 1 4 1
Nucleolus 3 0 0 3 4 2 6 – 11 10 13 5 3 0 5 0 0 1 17 26 10 15 22 7
Peroxisomes 0 0 0 0 0 1 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 6 14 17 15 26 16 28 – 18 18 11 19 1 2 1 6 1 0 12 29 12 7 12 4
Vac/Vac Membrane 9 33 12 2 5 8 6 – 6 6 2 9 10 6 11 76 71 46 21 87 42 30 49 53
Unique Cell Count 317 577 239 211 228 354 376 337 355 245 325 218 159 160 371 255 228 353 669 299 143 199 149
Labelled Cell Count 342 631 259 237 262 438 507 491 501 354 454 249 164 171 429 282 261 353 669 299 143 199 149


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.7 5.5 5.0 5.4 4.2 4.4 – 3.9 3.7 3.5 4.2 4.6 4.9 4.8 7.3 7.6 6.7 4.5 4.7 5.8
Std Deviation (1e-4) 0.6 0.7 1.1 1.4 1.3 1.0 1.0 – 1.0 0.9 0.9 1.0 1.0 1.4 1.4 1.4 1.4 1.4 1.3 1.6 2.6
Intensity Change (Log2) – – – -0.13 -0.04 -0.4 -0.33 – -0.51 -0.57 -0.66 -0.39 -0.27 -0.18 -0.22 0.4 0.47 0.29 -0.31 -0.24 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.3 6.8 5.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2712 0.1001 -0.1361 -0.4114 -0.6883 -0.0274 1.8259 1.2307 0.8138 0.9257 1.8247 1.1443 1.0442 0.5701 0.738 0.5948 0.7412 0.712
Actin 0.0005 0.0003 0.0001 0.0104 0.0002 0.0001 0.0292 0.0036 0.0001 0 0.0111 0.0021 0.0123 0.003 0.0038 0.0002 0.0024 0.0017
Bud 0.0022 0.0001 0.0001 0.0012 0 0.0001 0.0003 0.0001 0.0001 0 0.0003 0.0003 0.0007 0.0018 0.0001 0 0.0001 0
Bud Neck 0.001 0.0005 0.0004 0.0012 0.0012 0.0027 0.0028 0.0008 0.0005 0.0003 0.0025 0.0045 0.0054 0.0006 0.0025 0.0043 0.0011 0.0035
Bud Periphery 0.0004 0.0001 0 0.0027 0 0.0001 0.0005 0.0001 0.0001 0 0.0008 0.0002 0.0012 0.0003 0.0002 0 0.0001 0.0001
Bud Site 0.0009 0.0008 0.0002 0.0025 0.0001 0.0001 0.0013 0.0008 0.0001 0 0.0007 0.0001 0.0076 0.001 0.0016 0.0002 0.0003 0.0001
Cell Periphery 0.0001 0 0 0.0002 0 0 0.0002 0 0 0 0.0001 0 0.0004 0 0.0004 0 0.0001 0
Cytoplasm 0.0133 0.0178 0.0106 0.0034 0.0154 0.0069 0.0152 0.0088 0.005 0.0008 0.0074 0.0037 0.0067 0.0123 0.0062 0.002 0.0136 0.0034
Cytoplasmic Foci 0.0022 0.0024 0.0008 0.0008 0.0005 0.002 0.0023 0.0013 0.0002 0 0.0067 0.0007 0.0027 0.0008 0.0011 0.0003 0.0027 0.0007
Eisosomes 0.0001 0 0 0.0002 0 0 0.0003 0 0 0 0.0001 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0007 0.0013 0.002 0.0015 0.0008 0.0012 0.0058 0.0006 0.0003 0 0.0041 0.0008 0.0026 0.0009 0.0029 0.0003 0.0038 0.0005
Endosome 0.003 0.0018 0.0015 0.0028 0.0011 0.001 0.0188 0.0008 0.0005 0.0001 0.0324 0.0029 0.0076 0.0009 0.0025 0.0011 0.0146 0.0014
Golgi 0.0002 0.0001 0.0001 0.0007 0 0 0.0027 0.0002 0 0 0.0123 0.0005 0.002 0.0002 0.0008 0.0001 0.001 0.0006
Lipid Particles 0.0013 0.0003 0.0005 0.0007 0.0002 0.0002 0.0032 0.0002 0.0001 0 0.0073 0.0054 0.0022 0.0004 0.0016 0.0001 0.0159 0.0031
Mitochondria 0.0005 0.0003 0.0002 0.0109 0.0003 0.0002 0.003 0.0003 0.0003 0.0001 0.0088 0.0026 0.0031 0.0005 0.0004 0.0001 0.0013 0.0015
None 0.0243 0.0179 0.0319 0.0027 0.0234 0.0183 0.0253 0.0311 0.0106 0.0003 0.0094 0.0011 0.0086 0.0216 0.0187 0.0071 0.0132 0.0163
Nuclear Periphery 0.0297 0.0273 0.0293 0.0161 0.0171 0.0119 0.042 0.0112 0.0188 0.0041 0.0566 0.0167 0.0222 0.0125 0.0084 0.0059 0.0403 0.004
Nucleolus 0.0225 0.02 0.0235 0.0107 0.0309 0.0531 0.0128 0.0212 0.026 0.0159 0.0196 0.0375 0.0191 0.0215 0.015 0.0232 0.0244 0.0371
Nucleus 0.8484 0.8599 0.8383 0.8596 0.8106 0.8414 0.7838 0.8557 0.9072 0.9678 0.6903 0.8813 0.8322 0.8906 0.8737 0.8897 0.7933 0.8573
Peroxisomes 0.0003 0.0004 0.0001 0.0009 0 0 0.0009 0.0013 0 0 0.001 0.0008 0.0011 0.0001 0.0037 0 0.0012 0.0005
Punctate Nuclear 0.0473 0.0465 0.0594 0.0686 0.0978 0.0596 0.0452 0.0606 0.0296 0.0103 0.1232 0.0381 0.0587 0.0302 0.0552 0.0651 0.0659 0.0676
Vacuole 0.0006 0.002 0.0006 0.0014 0.0004 0.0007 0.0021 0.0011 0.0003 0.0001 0.0026 0.0004 0.0024 0.0007 0.001 0.0003 0.0028 0.0003
Vacuole Periphery 0.0004 0.0004 0.0003 0.0009 0.0001 0.0001 0.0024 0.0002 0.0002 0 0.0029 0.0004 0.001 0.0002 0.0003 0 0.0018 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.3194 13.3689 11.4704 14.3175 13.0276 9.3418 13.9397 17.2487 14.6589 14.4109
Translational Efficiency 0.1737 0.1741 0.2377 0.2687 0.1734 0.3326 0.2035 0.205 0.1863 0.2055

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Zinc finger transcription factor involved in multidrug resistance; Zn(2)-Cys(6) zinc finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Yrm1

Yrm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yrm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available