Standard name | |
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Human Ortholog | |
Description | Actin assembly factor; C-terminal WCA domain activates Arp2/3 complex-mediated nucleation of branched actin filaments, polyproline domain nucleates actin filaments independent of Arp2/3; mutants are defective in endocytosis, bud site selection, cytokinesis; human homolog WAS (Wiskott-Aldrich Syndrome) implicated in severe immunodeficiency; human WAS complements yeast null mutant, but only in presence of WIPF1, which mediates localization of WAS to cortical patches |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 124 | 101 | – | – | 13 | 51 | 84 | 92 | 57 | 43 | 39 | 53 | 57 | 153 | 127 | 23 | 25 | 40 | 158 | 116 | 22 | 99 | 170 | 180 |
Bud | 32 | 25 | – | – | 6 | 17 | 21 | 31 | 24 | 15 | 16 | 20 | 11 | 32 | 28 | 7 | 3 | 8 | 10 | 16 | 2 | 8 | 16 | 13 |
Bud Neck | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 13 | 0 | 13 | 20 | 19 |
Bud Site | 21 | 13 | – | – | 0 | 12 | 5 | 6 | 5 | 6 | 4 | 5 | 7 | 7 | 2 | 1 | 1 | 2 | – | – | – | – | – | – |
Cell Periphery | 16 | 24 | – | – | 1 | 4 | 10 | 19 | 12 | 11 | 4 | 5 | 12 | 37 | 48 | 5 | 7 | 8 | 3 | 2 | 0 | 0 | 2 | 5 |
Cytoplasm | 7 | 12 | – | – | 2 | 7 | 10 | 10 | 3 | 3 | 4 | 8 | 11 | 14 | 21 | 6 | 5 | 11 | 1 | 0 | 1 | 2 | 2 | 4 |
Endoplasmic Reticulum | 5 | 10 | – | – | 1 | 13 | 18 | 25 | 7 | 12 | 10 | 4 | 7 | 20 | 15 | 1 | 1 | 4 | 0 | 0 | 1 | 0 | 2 | 1 |
Endosome | 1 | 0 | – | – | 1 | 1 | 1 | 4 | 3 | 2 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 3 | 5 | 6 | 1 | 1 | 7 | 8 |
Golgi | 4 | 7 | – | – | 3 | 7 | 13 | 14 | 14 | 13 | 4 | 15 | 1 | 19 | 17 | 8 | 14 | 18 | 0 | 1 | 1 | 0 | 2 | 1 |
Mitochondria | 89 | 41 | – | – | 0 | 33 | 36 | 33 | 68 | 55 | 88 | 82 | 19 | 10 | 7 | 6 | 5 | 6 | 3 | 1 | 1 | 3 | 7 | 4 |
Nucleus | 0 | 1 | – | – | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 0 | – | – | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 2 | 0 | – | – | 1 | 1 | 1 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 66 | 32 | – | – | 3 | 30 | 30 | 27 | 34 | 23 | 15 | 21 | 16 | 71 | 70 | 11 | 18 | 20 | 9 | 4 | 4 | 6 | 12 | 10 |
SpindlePole | 3 | 2 | – | – | 1 | 3 | 4 | 10 | 7 | 3 | 1 | 5 | 2 | 9 | 10 | 4 | 5 | 16 | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | – | – | 1 | 3 | 3 | 3 | 4 | 4 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 4 | 1 | 0 | 0 | 2 | 6 | 2 |
Unique Cell Count | 182 | 140 | 19 | 104 | 137 | 149 | 142 | 106 | 110 | 131 | 81 | 207 | 208 | 45 | 48 | 90 | 204 | 169 | 40 | 143 | 256 | 257 | ||
Labelled Cell Count | 373 | 268 | 33 | 183 | 237 | 275 | 240 | 192 | 190 | 222 | 144 | 376 | 348 | 75 | 86 | 143 | 204 | 169 | 40 | 143 | 256 | 257 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 8.8 | – | – | 10.1 | 8.7 | 8.9 | 8.8 | 7.4 | 7.9 | 6.9 | 7.7 | 8.3 | 8.5 | 8.5 | 10.7 | 9.8 | 10.6 | 8.0 | 8.3 | 8.5 |
Std Deviation (1e-4) | 1.4 | 2.2 | – | – | 2.0 | 2.6 | 2.3 | 1.9 | 1.8 | 2.1 | 1.8 | 2.1 | 1.7 | 1.7 | 1.7 | 3.0 | 2.5 | 3.4 | 1.5 | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.3038 | 3.5225 | 2.8834 | 3.3591 | 2.2393 | 3.3846 | 3.3876 | 4.0146 | 2.946 | 2.4512 | 2.8675 | 3.2896 | 4.9502 | 3.8018 | 3.9478 | 4.3135 | 3.7675 | 3.8978 |
Actin | 0.7204 | 0.6878 | 0.8833 | 0.742 | 0.6041 | 0.8814 | 0.784 | 0.5966 | 0.7431 | 0.7687 | 0.8884 | 0.8021 | 0.873 | 0.6901 | 0.8991 | 0.9561 | 0.9368 | 0.8843 |
Bud | 0.0007 | 0.0014 | 0.0063 | 0.0067 | 0.0097 | 0.0015 | 0.001 | 0.0026 | 0.0159 | 0.0131 | 0.0022 | 0.0018 | 0.0028 | 0.0056 | 0.0087 | 0.0043 | 0.0022 | 0.0011 |
Bud Neck | 0.022 | 0.0099 | 0.0125 | 0.0383 | 0.004 | 0.0546 | 0.0117 | 0.0115 | 0.0341 | 0.0039 | 0.0008 | 0.1104 | 0.0122 | 0.0097 | 0.0184 | 0.0063 | 0.0459 | 0.0844 |
Bud Periphery | 0.001 | 0.0016 | 0.0128 | 0.0223 | 0.0275 | 0.0071 | 0.0015 | 0.0044 | 0.0189 | 0.0377 | 0.005 | 0.0133 | 0.0086 | 0.0056 | 0.0194 | 0.0111 | 0.0072 | 0.0054 |
Bud Site | 0.0328 | 0.276 | 0.0401 | 0.0034 | 0.0085 | 0.002 | 0.0362 | 0.3305 | 0.0944 | 0.0119 | 0.0011 | 0.0039 | 0.0365 | 0.2319 | 0.0417 | 0.0061 | 0.0023 | 0.0023 |
Cell Periphery | 0.0027 | 0.0033 | 0.002 | 0.0006 | 0.0028 | 0.0008 | 0.0015 | 0.0017 | 0.0012 | 0.0008 | 0.0001 | 0.0016 | 0.002 | 0.0012 | 0.0007 | 0.0013 | 0.0005 | 0.0007 |
Cytoplasm | 0.0025 | 0.0117 | 0.0038 | 0.0023 | 0.0681 | 0.0005 | 0.0038 | 0.0191 | 0.0023 | 0.0008 | 0.0008 | 0.0007 | 0.0012 | 0.0174 | 0.0082 | 0.0002 | 0.0001 | 0.0038 |
Cytoplasmic Foci | 0.0328 | 0.0019 | 0.0074 | 0.0344 | 0.0189 | 0.0055 | 0.028 | 0.0057 | 0.0095 | 0.0219 | 0.0169 | 0.0076 | 0.0086 | 0.0126 | 0.0004 | 0.0002 | 0.0008 | 0.0014 |
Eisosomes | 0.0036 | 0.0021 | 0.0033 | 0.0013 | 0.0006 | 0.0037 | 0.0139 | 0.0026 | 0.002 | 0.0013 | 0.0006 | 0.0079 | 0.0283 | 0.0021 | 0.0015 | 0.0133 | 0.0017 | 0.0091 |
Endoplasmic Reticulum | 0.0068 | 0.0001 | 0.0003 | 0.0012 | 0.0007 | 0.0021 | 0.0014 | 0.0003 | 0.0003 | 0.0022 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Endosome | 0.0242 | 0.0001 | 0.0011 | 0.0229 | 0.0278 | 0.0098 | 0.0294 | 0.0026 | 0.0177 | 0.0119 | 0.0282 | 0.0013 | 0.0033 | 0.0008 | 0.0001 | 0 | 0.0001 | 0.0008 |
Golgi | 0.0159 | 0.0006 | 0.0024 | 0.0236 | 0.0403 | 0.0066 | 0.0143 | 0.003 | 0.0129 | 0.0123 | 0.0026 | 0.0036 | 0.0033 | 0.0031 | 0.0003 | 0.0005 | 0.0007 | 0.0022 |
Lipid Particles | 0.0508 | 0.0002 | 0.0057 | 0.0216 | 0.0251 | 0.0017 | 0.0271 | 0.0003 | 0.0126 | 0.0372 | 0.0213 | 0.0025 | 0.0049 | 0.0006 | 0 | 0 | 0.0002 | 0.0002 |
Mitochondria | 0.0221 | 0.0002 | 0.0019 | 0.0159 | 0.1331 | 0.0024 | 0.0167 | 0.0004 | 0.0188 | 0.0133 | 0.0003 | 0.0281 | 0.0012 | 0.0006 | 0.0004 | 0.0001 | 0.0001 | 0.0004 |
None | 0.0158 | 0.0001 | 0.0046 | 0.0054 | 0.0004 | 0.0003 | 0.0023 | 0.0002 | 0.0008 | 0.002 | 0.0005 | 0.0003 | 0.0015 | 0.0125 | 0.0002 | 0 | 0 | 0.0001 |
Nuclear Periphery | 0.0031 | 0 | 0.0002 | 0.0071 | 0.0004 | 0.0043 | 0.001 | 0.0002 | 0.0002 | 0.0037 | 0.0032 | 0 | 0.0005 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.0013 | 0 | 0.0002 | 0.0029 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0006 | 0.0006 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0009 | 0 | 0.0001 | 0.0038 | 0.0003 | 0.0003 | 0.0004 | 0.0003 | 0.0001 | 0.0007 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0001 |
Peroxisomes | 0.0323 | 0.0029 | 0.0106 | 0.0274 | 0.023 | 0.0139 | 0.0206 | 0.004 | 0.0111 | 0.0519 | 0.0037 | 0.0141 | 0.0088 | 0.0057 | 0.0007 | 0.0003 | 0.0015 | 0.0022 |
Punctate Nuclear | 0.0064 | 0.0001 | 0.0007 | 0.015 | 0.0003 | 0.0006 | 0.0015 | 0.0001 | 0.0003 | 0.0015 | 0.0222 | 0.0001 | 0.0009 | 0.0002 | 0 | 0 | 0 | 0 |
Vacuole | 0.0012 | 0.0001 | 0.0004 | 0.0011 | 0.0024 | 0.0004 | 0.0021 | 0.0138 | 0.0032 | 0.0014 | 0.0004 | 0.0002 | 0.0012 | 0.0001 | 0.0001 | 0 | 0 | 0.0013 |
Vacuole Periphery | 0.0009 | 0 | 0.0001 | 0.0008 | 0.0017 | 0.0004 | 0.0009 | 0.0003 | 0.0006 | 0.001 | 0.0002 | 0.0002 | 0.0003 | 0 | 0 | 0 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.9231 | 10.372 | 15.7778 | 18.2679 | 15.7954 | 13.9154 | 20.8431 | 19.6417 | 19.0277 | 16.6858 |
Translational Efficiency | 1.9913 | 2.5065 | 1.5799 | 1.2949 | 1.6442 | 2.401 | 1.4289 | 1.6195 | 1.8093 | 1.606 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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