Standard name
Human Ortholog
Description GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); not required for viability; protein abundance increases in response to DNA replication stress; GSP2 has a paralog, GSP1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.75 0.7 0.62 0.31 0.29 0.34 0.21 0.22 0.21 0.22 0.26 0.25 0.28 0.2 0.19 0.34 0.26 0.34 0.67 0.56 0.53 0.53 0.4 0.38
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.08 0.1 0.13 0.13 0.23 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.05 0 0 0.21 0.12 0.19 0.19 0.23 0.29 0.28 0.29 0.29 0.14 0.07 0.08 0.05 0 0 0.1 0.23 0.14 0.13 0.15 0.17
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.37 0.39 0.51 0.56 0.64 0.49 0.61 0.51 0.48 0.43 0.35 0.35 0.75 0.77 0.76 0.75 0.81 0.76 0.1 0.11 0.15 0.19 0.27 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 1 1 0 0 1 1 1 0 0 0 1 0 1 1 1
Bud 0 1 0 0 0 0 3 4 5 4 2 2 0 0 0 0 0 0 5 3 3 11 23 34
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 4 4
Bud Site 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 1 0 2 0 0 1 2 0 0 0 0 0 0 0 0
Cytoplasm 209 128 155 74 90 102 78 113 73 84 67 68 104 117 109 126 81 105 218 115 154 215 182 184
Endoplasmic Reticulum 2 2 0 1 2 1 0 4 1 2 4 3 2 3 3 7 15 7 1 2 7 5 4 6
Endosome 0 0 1 1 0 0 0 0 1 6 0 2 2 16 12 1 0 0 5 1 7 6 6 7
Golgi 0 0 0 0 2 0 0 0 0 1 0 0 0 3 5 3 11 4 1 1 0 3 1 1
Mitochondria 4 1 8 10 11 25 32 49 43 51 60 47 1 0 0 4 1 0 3 0 6 11 11 8
Nucleus 14 5 9 51 38 56 71 117 98 109 76 79 51 42 47 17 6 5 33 47 38 50 69 80
Nuclear Periphery 0 0 0 1 0 1 0 6 2 3 2 5 0 2 0 0 0 0 6 1 12 1 2 3
Nucleolus 1 0 0 1 0 2 7 15 5 10 6 3 0 5 8 1 0 0 1 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 8 4 9 9 15 9
Vac/Vac Membrane 103 71 128 134 199 148 232 262 162 166 90 95 281 450 442 276 251 233 32 23 41 75 123 138
Unique Cell Count 280 182 250 241 312 301 378 512 341 383 259 275 376 582 585 367 309 307 325 208 288 404 459 489
Labelled Cell Count 334 208 301 273 342 335 423 571 391 438 309 307 441 640 629 438 365 354 325 208 288 404 459 489


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 5.9 5.1 6.1 8.4 10.1 9.9 10.8 10.5 10.7 11.2 12.3 8.9 11.8 13.4 23.0 23.5 23.7 7.7 8.2 8.8
Std Deviation (1e-4) 0.8 0.9 1.2 1.8 2.1 2.8 3.3 3.7 3.9 4.3 4.6 5.2 2.2 4.0 4.3 8.0 7.0 6.9 3.7 3.4 2.1
Intensity Change (Log2) – – – 0.27 0.74 1.0 0.97 1.09 1.06 1.08 1.15 1.29 0.82 1.23 1.41 2.18 2.22 2.23 0.6 0.7 0.8


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -6.9 -7.9 -6.6 -10.5 -10.8 -10.0 -10.2 -8.2 -8.6 -8.5 -11.8 -12.3 -6.8 -8.5 -6.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.5 0
Endosome 0 0 0 0 0 0 0 0 0 0 2.2 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.6 0.2 2.5 2.6 3.1 4.0 4.3 6.6 5.2 0 0 0 0 0 0
Nucleus 5.9 3.7 5.4 5.6 6.7 7.8 7.9 7.8 7.7 4.1 2.0 2.3 0.6 -1.2 -1.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.0 3.0 -0.5 2.5 0 -0.9 -1.9 -3.7 -3.9 6.1 7.5 6.9 6.2 7.6 6.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0083 0.0002 0.0007 0.0178 0.0064 0.0003 0.0084 0.0016 0.0042 0.0016 0.0209 0.0007 0.2742 0.0006 0.0151 0.0006 0.0002 0.0008
Bud 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0.0006 0.0008 0.0007 0.0021 0.0005 0.0001 0.0044 0.0002 0.0004 0 0.0001 0.0002
Bud Neck 0.0016 0.0004 0.0006 0.0022 0.0013 0.0026 0.0054 0.0005 0.0005 0.0032 0.0009 0.0011 0.0019 0.0003 0.0005 0.0003 0.0005 0.002
Bud Periphery 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0014 0.0004 0.0006 0.0011 0.0006 0.0001 0.01 0.0001 0.0002 0 0 0.0002
Bud Site 0.0007 0.0005 0.0002 0.0002 0.0002 0.0009 0.004 0.0021 0.0045 0.0134 0.0009 0.0001 0.0074 0.0006 0.0032 0 0.0001 0.0001
Cell Periphery 0.0001 0.0001 0 0 0 0 0.0002 0.0001 0.0001 0.0002 0.0001 0 0.0009 0.0001 0.0001 0 0 0
Cytoplasm 0.0544 0.1541 0.1016 0.0676 0.0646 0.0764 0.1097 0.3347 0.1467 0.1091 0.1848 0.126 0.1506 0.4584 0.31 0.2063 0.1512 0.1237
Cytoplasmic Foci 0.0059 0.0006 0.0005 0.0049 0.0117 0.001 0.0069 0.0038 0.0044 0.0193 0.0091 0.0032 0.0398 0.0015 0.0336 0.0002 0.0002 0.0041
Eisosomes 0.0003 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0.0024 0 0 0 0 0
Endoplasmic Reticulum 0.0069 0.0045 0.0059 0.0221 0.0027 0.0045 0.0124 0.0126 0.0111 0.0081 0.007 0.0079 0.013 0.0238 0.01 0.0212 0.0022 0.0046
Endosome 0.0128 0.0022 0.0034 0.0091 0.0115 0.0045 0.0231 0.0058 0.0103 0.0398 0.0197 0.0048 0.0192 0.0049 0.0548 0.0022 0.0008 0.0148
Golgi 0.002 0 0 0.0011 0.0168 0.0001 0.002 0.0002 0.0024 0.0124 0.0135 0.0006 0.0143 0.0001 0.0041 0 0 0.0073
Lipid Particles 0.0051 0 0 0.0008 0.0262 0.0001 0.0021 0.0001 0.0005 0.017 0.0053 0.0009 0.007 0.0003 0.0077 0 0 0.0345
Mitochondria 0.0014 0.0005 0.0004 0.0007 0.0029 0.0003 0.0045 0.0007 0.0016 0.0064 0.0045 0.0007 0.0312 0.0007 0.0015 0.0005 0.0007 0.0091
None 0.004 0.0039 0.0009 0.002 0.0005 0.002 0.0177 0.049 0.0206 0.0026 0.0418 0.0213 0.0063 0.0044 0.0047 0.0007 0.0012 0.0022
Nuclear Periphery 0.1504 0.1 0.1773 0.1258 0.0591 0.0913 0.1809 0.1092 0.1766 0.1467 0.0724 0.106 0.0332 0.0936 0.1577 0.2496 0.0365 0.0585
Nucleolus 0.0015 0.0006 0.0006 0.0005 0.0009 0.0021 0.0049 0.0009 0.0008 0.0033 0.0006 0.0006 0.0006 0.0004 0.0009 0.0001 0.0005 0.001
Nucleus 0.7258 0.7248 0.6958 0.7289 0.778 0.7951 0.5906 0.4611 0.5982 0.5646 0.5848 0.7058 0.3392 0.3972 0.3573 0.5123 0.8018 0.7238
Peroxisomes 0.0012 0 0 0.0008 0.0078 0 0.0005 0.0002 0.0021 0.0049 0.0034 0.0003 0.0296 0 0.0118 0 0 0.0028
Punctate Nuclear 0.0145 0.0029 0.0083 0.0126 0.0068 0.0158 0.0118 0.0101 0.0089 0.032 0.0262 0.0171 0.004 0.0075 0.0151 0.0019 0.0025 0.0061
Vacuole 0.0021 0.0034 0.0021 0.0017 0.0019 0.0018 0.0089 0.0047 0.0037 0.0091 0.0019 0.0017 0.004 0.004 0.0084 0.0019 0.0011 0.0024
Vacuole Periphery 0.0012 0.0009 0.0011 0.001 0.0006 0.0007 0.0039 0.0013 0.0016 0.0028 0.0009 0.001 0.0067 0.0012 0.003 0.002 0.0005 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.1246 32.7304 30.9112 47.6966 44.2346 48.2368 42.1193 53.3019 69.1604 57.4238
Translational Efficiency 2.0536 1.5258 1.6945 1.5356 1.393 1.6018 1.5162 1.3867 1.412 1.4719

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); not required for viability; protein abundance increases in response to DNA replication stress; GSP2 has a paralog, GSP1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (19%), nucleus (8%), mixed (39%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Gsp2

Gsp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gsp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available