Standard name
Human Ortholog
Description Mitochondrial translation elongation factor Tu (EF-Tu); involved in fundamental pathway of mtDNA homeostasis; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans; rare mutations in human mitochondrial elongation factor Tu (EFTu) associated with severe lactic acidosis, rapidly progressive fatal encephalopathy, severe infantile macrocystic leukodystrophy with micropolygyria
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.19 0.24 0.1 0 0.06 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Cytoplasm 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.15 – 0 0 0.05 0 0 0 0 0 0 0 0.25 0.61 0.57 0.75 0.69 0.65 0.12 0.07 0 0.09 0.16 0.14
Mitochondria 0.95 – 0.88 0.96 0.87 0.85 0.9 0.86 0.88 0.91 0.95 0.89 0.79 0.66 0.68 0.09 0.06 0.13 0.7 0.7 0.85 0.65 0.62 0.6
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0.08 0.08 0.08 0.1 0.13 0.1 0.14 0.08 0.09 0.12 0 0.06 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.15 – 0.05 0 0.07 0.16 0.19 0.16 0.17 0.15 0.12 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0.15 – 0.06 0 0.09 0 0 0 0.05 0 0 0 0.18 0.18 0.11 0.11 0.12 0.16 0 0.07 0 0.15 0.15 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 6 2 3 0 2 3 4 4 0 1 3 11 18 17 35 16 0 2 2 1 1 4
Bud 0 – 1 1 3 1 2 4 7 10 1 3 0 0 1 4 1 5 0 0 0 0 0 1
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 1 0 0 3 4 1 4 2 2 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 – 3 0 0 1 1 1 3 0 0 0 2 6 4 6 7 7 0 0 1 0 0 0
Cytoplasm 0 – 2 3 2 1 3 5 3 2 0 3 0 2 3 5 11 11 0 0 0 0 0 0
Endoplasmic Reticulum 0 – 1 0 3 2 0 1 0 0 0 2 0 2 3 2 7 4 1 1 0 0 0 0
Endosome 0 – 2 0 1 0 1 3 1 1 1 1 1 1 4 2 3 4 1 1 1 1 0 4
Golgi 3 – 5 2 8 0 0 0 2 2 0 1 17 94 118 66 100 108 5 2 2 4 18 19
Mitochondria 19 – 110 154 137 130 296 264 254 457 283 285 53 102 141 8 9 21 33 29 111 30 71 84
Nucleus 0 – 0 0 0 0 2 3 1 2 1 2 0 1 0 0 2 1 0 0 0 0 1 0
Nuclear Periphery 0 – 10 13 12 16 42 32 40 42 26 39 1 9 4 0 1 2 0 0 0 0 0 0
Nucleolus 3 – 6 7 11 25 64 50 48 73 35 44 2 5 8 1 0 7 0 0 0 1 0 1
Peroxisomes 0 – 3 1 1 1 5 8 4 5 2 1 0 1 2 0 1 1 0 0 7 0 2 0
SpindlePole 0 – 0 1 3 0 2 4 2 1 2 0 0 0 4 0 1 8 0 0 1 0 0 1
Vac/Vac Membrane 3 – 8 4 14 5 3 3 15 10 4 8 12 28 22 10 17 26 1 2 1 6 16 16
Unique Cell Count 20 125 161 157 153 329 306 289 500 299 319 67 155 206 88 144 165 48 42 131 47 116 140
Labelled Cell Count 28 157 189 198 182 426 385 385 613 357 392 91 262 332 121 195 222 48 42 131 47 116 140


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.8 – 15.4 13.7 14.1 13.0 12.7 12.9 12.9 12.3 12.4 12.7 16.0 16.4 16.2 19.2 19.1 19.1 12.1 18.0 17.7
Std Deviation (1e-4) 2.7 – 3.2 3.0 3.0 2.9 3.0 3.2 3.2 3.3 3.9 3.7 3.3 3.0 3.3 4.7 5.4 5.6 3.1 4.5 4.4
Intensity Change (Log2) – – – -0.18 -0.13 -0.25 -0.29 -0.26 -0.26 -0.33 -0.32 -0.28 0.05 0.09 0.07 0.31 0.31 0.3 -0.35 0.22 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.8 1.3 3.4 4.4 1.6
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 2.3 2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.4 0 0 0 0 0 0 0 4.4 9.9 9.7 10.8 11.0 10.6
Mitochondria 2.4 -0.2 -0.7 0.6 -0.5 0 1.2 2.4 0.4 -1.6 -4.3 -4.0 -11.4 -13.5 -12.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 -0.1 0.7 1.4 0.8 1.7 0.1 0.2 1.3 0 -0.7 -2.7 0 -3.0 -2.9
Nucleolus -0.2 0.8 3.0 3.9 3.2 3.3 2.9 2.2 2.7 0 0 -0.4 0 0 -0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.6 0.8 -1.2 0 0 -0.5 0 0 0 2.5 2.9 1.3 1.3 1.5 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 24.8402 29.8691 27.5917 26.5717 24.8407 27.3905 28.1724 30.9152 28.2138 26.4194 23.4436 28.5826 23.9972 25.9568 24.5994 21.3284 17.109 25.7476
Actin 0.0438 0.005 0.0096 0.0015 0.0032 0.0075 0.0557 0.0203 0.0118 0.0272 0.0073 0.0027 0.0121 0.0163 0.012 0.018 0.0023 0.0006
Bud 0.001 0.0004 0.0002 0.001 0.0001 0 0.0003 0.0006 0.0004 0.0012 0.002 0.0001 0.0004 0.0015 0.0002 0.0013 0.0004 0
Bud Neck 0.0003 0.0001 0 0.0005 0 0 0.0002 0.0002 0.0003 0.0009 0.0006 0.0001 0.0001 0.0006 0.0002 0.0008 0.0001 0
Bud Periphery 0.0028 0.0009 0.0006 0.004 0.0002 0.0001 0.0006 0.0018 0.0006 0.0027 0.0014 0.0004 0.0012 0.002 0.0002 0.003 0.0009 0
Bud Site 0.0015 0.0004 0.0003 0.0032 0.0001 0 0.0008 0.0029 0.0017 0.0026 0.0592 0 0.001 0.0078 0.0039 0.0058 0.0004 0
Cell Periphery 0.0004 0.0005 0.0002 0.0084 0.0001 0.0001 0.0006 0.0008 0.0007 0.0009 0.0003 0.0003 0.0002 0.0009 0.0002 0.0028 0.0006 0.0003
Cytoplasm 0.0004 0 0.0001 0.0015 0 0 0.0001 0 0.0001 0.0001 0.0015 0 0.0003 0.0001 0.0001 0.0004 0.0003 0
Cytoplasmic Foci 0.0404 0.0011 0.0015 0.0043 0.0001 0.0002 0.0045 0.0006 0.0037 0.0041 0.0294 0.0001 0.0052 0.0015 0.003 0.0227 0.0092 0.0001
Eisosomes 0.0026 0.0005 0.0014 0.0007 0.0004 0.0005 0.0015 0.0014 0.0029 0.0013 0.0001 0.0006 0.0011 0.0007 0.001 0.0006 0.0006 0.0005
Endoplasmic Reticulum 0.0017 0.0004 0.0002 0.0053 0 0.0001 0.0003 0.0001 0.0001 0.0005 0.0002 0.0012 0.0003 0.0002 0.0001 0.001 0.0004 0.0012
Endosome 0.0053 0.0046 0.0007 0.0079 0 0.0011 0.0023 0.0028 0.0106 0.0118 0.0086 0.0018 0.0038 0.0096 0.0051 0.0111 0.0188 0.0023
Golgi 0.0056 0.0197 0.0027 0.0015 0.0002 0.0034 0.0062 0.018 0.0425 0.0207 0.0185 0.0269 0.0045 0.0484 0.0092 0.0156 0.0184 0.0391
Lipid Particles 0.0296 0.0112 0.0044 0.0766 0.0006 0.0113 0.0158 0.0089 0.0066 0.035 0.0136 0.0008 0.0105 0.0182 0.0059 0.0415 0.0471 0.001
Mitochondria 0.8208 0.9031 0.9558 0.7376 0.9926 0.9602 0.8664 0.8938 0.9038 0.8732 0.8214 0.9531 0.9419 0.8505 0.9402 0.8129 0.8434 0.9528
None 0.0012 0.0001 0.0146 0.0026 0 0 0.0003 0 0.0001 0.0002 0.0003 0 0.0008 0.0004 0.0002 0.0007 0.0002 0
Nuclear Periphery 0.0043 0.0005 0.0001 0.0093 0 0.0008 0.0006 0.0006 0.0001 0.0009 0.0001 0.001 0.0027 0.0005 0.0004 0.0018 0.0004 0.0001
Nucleolus 0.0037 0.0006 0.001 0.0358 0.0007 0.0001 0.0021 0.0007 0.0018 0.0006 0.0003 0.0001 0.0007 0.0026 0.0002 0.0063 0.0006 0
Nucleus 0.0008 0.0003 0.0002 0.0155 0.0002 0.0001 0.0009 0.0003 0.0004 0.0004 0.0002 0.0001 0.0004 0.0011 0.0001 0.0026 0.0004 0
Peroxisomes 0.0192 0.0017 0.0019 0.0027 0.0007 0.0014 0.0039 0.0012 0.003 0.0047 0.0326 0.0005 0.0031 0.0051 0.007 0.0228 0.0033 0.0003
Punctate Nuclear 0.0047 0.0001 0.0015 0.0012 0 0 0.0074 0 0.0001 0.0004 0.0006 0 0.0008 0.0002 0.0008 0.0013 0.0001 0
Vacuole 0.0029 0.0079 0.0008 0.047 0.0001 0.0013 0.0087 0.0093 0.0034 0.0029 0.0015 0.0009 0.0017 0.009 0.0015 0.0107 0.0068 0.0002
Vacuole Periphery 0.007 0.0412 0.0021 0.0318 0.0008 0.0118 0.0207 0.0357 0.0053 0.0076 0.0005 0.0094 0.0073 0.0227 0.0084 0.0163 0.0453 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 162.4876 144.8787 120.8749 135.3057 130.6495 156.1032 170.3951 159.573 188.1175 187.5503
Translational Efficiency 1.1841 1.1446 1.1533 1.3255 1.2356 1.4425 1.2364 1.2014 1.1461 1.108

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial translation elongation factor Tu (EF-Tu); involved in fundamental pathway of mtDNA homeostasis; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans; rare mutations in human mitochondrial elongation factor Tu (EFTu) associated with severe lactic acidosis, rapidly progressive fatal encephalopathy, severe infantile macrocystic leukodystrophy with micropolygyria
Localization
Cell Percentages mitochondrion (71%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Tuf1

Tuf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tuf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available