Standard name | |
---|---|
Human Ortholog | |
Description | Peripheral peroxisomal membrane protein; involved in controlling peroxisome size and number, interacts with Pex25p; PEX27 has a paralog, PEX25, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 13 | 61 | 12 | 15 | 6 | 6 | 18 | 4 | 8 | 0 | 10 | 51 | 18 | 4 | 4 | 8 | 14 | 11 | 44 | 55 | 84 | 147 | 176 |
Bud | 26 | 15 | 34 | 45 | 54 | 66 | 81 | 87 | 57 | 62 | 64 | 65 | 41 | 43 | 24 | 18 | 13 | 22 | 3 | 3 | 2 | 7 | 11 | 12 |
Bud Neck | 1 | 2 | 55 | 30 | 38 | 34 | 20 | 40 | 22 | 16 | 14 | 19 | 42 | 25 | 13 | 13 | 8 | 10 | 5 | 6 | 8 | 12 | 20 | 18 |
Bud Site | 16 | 2 | 8 | 11 | 8 | 13 | 21 | 21 | 14 | 13 | 19 | 13 | 6 | 1 | 2 | 1 | 4 | 12 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 1 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 3 |
Cytoplasm | 24 | 51 | 80 | 109 | 150 | 161 | 198 | 164 | 123 | 161 | 116 | 138 | 140 | 190 | 171 | 103 | 33 | 53 | 2 | 7 | 9 | 16 | 19 | 14 |
Endoplasmic Reticulum | 0 | 4 | 10 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 0 | 1 | 6 | 0 | 0 | 9 | 4 | 8 | 0 | 1 | 2 | 3 | 2 | 3 |
Endosome | 8 | 18 | 4 | 9 | 15 | 28 | 10 | 13 | 6 | 4 | 0 | 17 | 26 | 91 | 224 | 42 | 21 | 38 | 50 | 114 | 110 | 85 | 127 | 107 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 1 | 0 | 1 | 6 | 4 | 2 | 7 | 2 |
Mitochondria | 116 | 79 | 6 | 20 | 40 | 151 | 244 | 145 | 225 | 200 | 325 | 195 | 13 | 8 | 3 | 20 | 14 | 25 | 26 | 42 | 68 | 93 | 105 | 92 |
Nucleus | 1 | 1 | 0 | 2 | 0 | 3 | 1 | 3 | 1 | 5 | 0 | 1 | 2 | 1 | 2 | 3 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 2 | 1 |
Nucleolus | 2 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Peroxisomes | 79 | 54 | 17 | 54 | 38 | 72 | 35 | 29 | 29 | 15 | 14 | 32 | 81 | 141 | 262 | 43 | 29 | 50 | 131 | 90 | 209 | 314 | 384 | 427 |
SpindlePole | 53 | 47 | 251 | 208 | 188 | 188 | 183 | 237 | 104 | 130 | 91 | 157 | 330 | 370 | 374 | 236 | 95 | 129 | 26 | 38 | 55 | 61 | 70 | 78 |
Vac/Vac Membrane | 2 | 2 | 2 | 1 | 4 | 3 | 2 | 2 | 4 | 4 | 6 | 6 | 0 | 7 | 5 | 12 | 1 | 4 | 2 | 7 | 2 | 2 | 3 | 4 |
Unique Cell Count | 223 | 225 | 406 | 397 | 436 | 528 | 616 | 561 | 431 | 471 | 474 | 486 | 587 | 719 | 817 | 408 | 169 | 277 | 265 | 374 | 540 | 695 | 918 | 957 |
Labelled Cell Count | 331 | 294 | 531 | 503 | 551 | 728 | 804 | 763 | 591 | 622 | 650 | 654 | 742 | 898 | 1089 | 507 | 234 | 369 | 265 | 374 | 540 | 695 | 918 | 957 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 4.7 | 5.0 | 4.7 | 4.5 | 3.7 | 3.5 | 4.0 | 3.3 | 3.5 | 3.1 | 3.6 | 5.0 | 5.4 | 5.4 | 6.1 | 5.4 | 5.2 | 4.9 | 5.0 | 5.4 |
Std Deviation (1e-4) | 0.5 | 1.1 | 1.1 | 1.6 | 1.4 | 1.4 | 1.2 | 1.1 | 1.1 | 1.1 | 1.0 | 1.0 | 1.0 | 1.4 | 1.3 | 1.6 | 1.7 | 1.4 | 1.5 | 1.6 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.16 | -0.42 | -0.52 | -0.32 | -0.59 | -0.51 | -0.7 | -0.48 | -0.01 | 0.11 | 0.12 | 0.29 | 0.12 | 0.05 | -0.02 | 0.01 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.7 | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.3793 | 0.6646 | 0.7987 | 0.7263 | 0.4115 | 0.8562 | 0.01 | -0.2031 | 0.1194 | -0.3852 | 0.0283 | -0.0984 | 0.3351 | -0.6218 | -0.5217 | -0.325 | 0.7386 | -0.6687 |
Actin | 0.0618 | 0.026 | 0.0431 | 0.0054 | 0.0074 | 0.0253 | 0.0402 | 0.0219 | 0.0395 | 0.008 | 0.0284 | 0.0149 | 0.0982 | 0.0354 | 0.0612 | 0.0025 | 0.0049 | 0.0293 |
Bud | 0.0014 | 0.0061 | 0.0047 | 0.0003 | 0.0005 | 0.0026 | 0.0004 | 0.0019 | 0.0007 | 0.0001 | 0.0005 | 0.0002 | 0.0008 | 0.0132 | 0.0158 | 0.0005 | 0.0011 | 0.0008 |
Bud Neck | 0.0032 | 0.0028 | 0.014 | 0.0308 | 0.0031 | 0.0064 | 0.0052 | 0.0023 | 0.0053 | 0.0006 | 0.0109 | 0.0106 | 0.02 | 0.0029 | 0.007 | 0.0331 | 0.0224 | 0.013 |
Bud Periphery | 0.0032 | 0.0064 | 0.0072 | 0.001 | 0.001 | 0.0021 | 0.0006 | 0.0037 | 0.0019 | 0.0002 | 0.0009 | 0.0004 | 0.0011 | 0.0043 | 0.01 | 0.0022 | 0.0011 | 0.0011 |
Bud Site | 0.0068 | 0.0223 | 0.0172 | 0.0013 | 0.0024 | 0.0015 | 0.0044 | 0.0155 | 0.0035 | 0.0005 | 0.0021 | 0.0006 | 0.0135 | 0.0479 | 0.0125 | 0.0008 | 0.0013 | 0.0015 |
Cell Periphery | 0.0005 | 0.001 | 0.0008 | 0.0009 | 0.0001 | 0.001 | 0.0002 | 0.0006 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0011 | 0.0005 | 0.0002 | 0.0008 | 0.0006 |
Cytoplasm | 0.0158 | 0.0339 | 0.0196 | 0.0098 | 0.0436 | 0.02 | 0.019 | 0.0295 | 0.0169 | 0.005 | 0.0141 | 0.0193 | 0.0235 | 0.0681 | 0.0199 | 0.036 | 0.059 | 0.0296 |
Cytoplasmic Foci | 0.2299 | 0.2315 | 0.1809 | 0.2339 | 0.1623 | 0.2617 | 0.3192 | 0.265 | 0.277 | 0.2622 | 0.2718 | 0.271 | 0.315 | 0.2826 | 0.2593 | 0.195 | 0.2532 | 0.2682 |
Eisosomes | 0.0009 | 0.0011 | 0.001 | 0.0008 | 0.0003 | 0.0007 | 0.0007 | 0.001 | 0.0006 | 0.0002 | 0.0005 | 0.0007 | 0.0007 | 0.0005 | 0.0003 | 0.0006 | 0.0001 | 0.0008 |
Endoplasmic Reticulum | 0.0066 | 0.0013 | 0.002 | 0.0056 | 0.0003 | 0.0011 | 0.0041 | 0.0009 | 0.0024 | 0.0001 | 0.0018 | 0.0007 | 0.0032 | 0.0021 | 0.0068 | 0.0006 | 0.0025 | 0.0038 |
Endosome | 0.0423 | 0.0273 | 0.0319 | 0.0308 | 0.0239 | 0.0263 | 0.0302 | 0.0294 | 0.0306 | 0.0159 | 0.0361 | 0.0171 | 0.0596 | 0.0597 | 0.0258 | 0.0175 | 0.1011 | 0.0571 |
Golgi | 0.018 | 0.0123 | 0.0168 | 0.0122 | 0.0109 | 0.0126 | 0.0106 | 0.0087 | 0.0121 | 0.0078 | 0.0106 | 0.0061 | 0.0281 | 0.0242 | 0.0133 | 0.0137 | 0.0611 | 0.0305 |
Lipid Particles | 0.0552 | 0.0252 | 0.0366 | 0.0389 | 0.0504 | 0.0344 | 0.0673 | 0.0433 | 0.0291 | 0.0625 | 0.0643 | 0.0551 | 0.0699 | 0.0328 | 0.0353 | 0.0207 | 0.0703 | 0.1188 |
Mitochondria | 0.0223 | 0.0245 | 0.0205 | 0.0403 | 0.0268 | 0.0223 | 0.0072 | 0.0071 | 0.0129 | 0.0028 | 0.0352 | 0.0091 | 0.0189 | 0.0129 | 0.0225 | 0.1458 | 0.0167 | 0.0186 |
None | 0.092 | 0.2117 | 0.1781 | 0.0757 | 0.1354 | 0.1669 | 0.2095 | 0.3033 | 0.2251 | 0.1574 | 0.2287 | 0.2002 | 0.0908 | 0.2035 | 0.1244 | 0.1144 | 0.0342 | 0.1094 |
Nuclear Periphery | 0.0126 | 0.0013 | 0.0012 | 0.0008 | 0.0009 | 0.0038 | 0.0079 | 0.0005 | 0.0107 | 0.0001 | 0.0117 | 0.005 | 0.0272 | 0.0006 | 0.0126 | 0.0001 | 0.0625 | 0.002 |
Nucleolus | 0.0057 | 0.0038 | 0.001 | 0.0012 | 0.008 | 0.0008 | 0.0013 | 0.0018 | 0.0006 | 0.0002 | 0.0008 | 0.0008 | 0.0009 | 0.001 | 0.0027 | 0.0004 | 0.0019 | 0.0009 |
Nucleus | 0.0135 | 0.0035 | 0.0018 | 0.0015 | 0.0019 | 0.0027 | 0.0031 | 0.003 | 0.0027 | 0.0001 | 0.0046 | 0.0021 | 0.0169 | 0.0011 | 0.0104 | 0.0002 | 0.0224 | 0.0018 |
Peroxisomes | 0.3865 | 0.3388 | 0.4091 | 0.4984 | 0.4583 | 0.3938 | 0.2465 | 0.2387 | 0.313 | 0.4736 | 0.2552 | 0.3742 | 0.1961 | 0.1981 | 0.347 | 0.4139 | 0.2547 | 0.2958 |
Punctate Nuclear | 0.0169 | 0.0165 | 0.0105 | 0.007 | 0.0607 | 0.012 | 0.02 | 0.0198 | 0.0126 | 0.0024 | 0.0201 | 0.0096 | 0.0121 | 0.0051 | 0.0087 | 0.0007 | 0.0084 | 0.0122 |
Vacuole | 0.0032 | 0.0021 | 0.0015 | 0.0029 | 0.0012 | 0.0015 | 0.0017 | 0.0019 | 0.0012 | 0.0002 | 0.001 | 0.0013 | 0.0014 | 0.0026 | 0.0029 | 0.0008 | 0.0116 | 0.0029 |
Vacuole Periphery | 0.0017 | 0.0006 | 0.0006 | 0.0007 | 0.0007 | 0.0006 | 0.0007 | 0.0003 | 0.0013 | 0.0001 | 0.0006 | 0.0007 | 0.0019 | 0.0005 | 0.001 | 0.0003 | 0.0085 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.195 | 7.8491 | 2.5886 | 6.9243 | 4.4272 | 9.7506 | 9.6999 | 10.4153 | 9.3201 | 9.5955 |
Translational Efficiency | 0.2761 | 0.5183 | 1.0898 | 0.563 | 0.8417 | 0.4643 | 0.5207 | 0.5448 | 0.4235 | 0.5772 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|