Standard name
Human Ortholog
Description Kinetochore-associated protein; required for chromosome segregation and kinetochore clustering; required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.06 0.05 – 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0.05 0.06 0 0.08 0 0 0.13 – 0.19 0.1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0.06 0.05 0.11 0.08 – 0.06 0.06 0.05 0.09 0.11 0.08 0.07 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.06 0 0 0 0 0 0 0 – 0 0 0.11 0.21 0.1 0.18 0.25 0.28 0.08 0.11 0.1 0.07 0.05 0.07
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.16 0.05 0 0 0.1 0.11 0.08 0.24 – 0.17 0.13 0.1 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.05 0.05 0 0.06 0.05 0.05 0.07 0.07 0.09 – 0.08 0.09 0 0.1 0.14 0.13 0.15 0.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.67 0.4 0.25 0.28 0.38 0.35 0.33 0.26 0.31 – 0.29 0.29 0.63 0.46 0.43 0.55 0.59 0.56 0 0.07 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0.12 0.09 0 0.05 0 0 0 0 0.05 0 0
SpindlePole 0.82 0.65 0.87 0.81 0.82 0.79 0.75 0.7 0.6 – 0.69 0.71 0.6 0.53 0.45 0.51 0.59 0.5 0.81 0.65 0.76 0.73 0.74 0.64
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 2 1 0 0 0 4 0 – 0 1 0 0 0 0 0 0 0 0 1 0 1 2
Bud 0 6 3 2 7 7 10 24 14 – 28 20 2 0 0 1 1 0 0 5 1 5 11 15
Bud Neck 1 7 10 4 4 6 14 18 7 – 15 12 0 1 0 0 0 0 1 4 1 3 2 5
Bud Site 1 10 14 13 13 20 18 14 33 – 65 30 9 2 6 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 2 0 – 0 1 0 0 1 0 0 1 0 0 0 0 0 0
Cytoplasm 0 12 9 11 14 15 21 45 20 – 19 18 14 39 36 18 15 11 0 2 0 1 2 5
Endoplasmic Reticulum 0 1 0 0 1 0 1 0 0 – 0 0 0 0 1 2 3 3 0 0 0 0 3 1
Endosome 2 23 5 6 3 3 2 1 0 – 0 0 31 94 33 38 55 39 7 41 31 14 16 20
Golgi 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 3 0 1 0 7 1 0 2 2
Mitochondria 3 56 14 7 7 26 47 32 62 – 57 38 27 6 7 8 6 6 0 12 3 1 5 9
Nucleus 3 17 12 12 16 12 28 29 23 – 26 27 10 44 46 29 32 14 1 6 4 2 11 7
Nuclear Periphery 0 4 0 0 0 0 1 0 1 – 1 0 0 0 0 2 0 0 0 1 0 0 1 0
Nucleolus 40 143 70 57 117 89 139 104 82 – 96 87 173 203 146 119 130 78 2 26 10 4 5 3
Peroxisomes 2 11 4 1 1 4 2 4 2 – 3 0 7 54 29 4 10 5 0 6 2 10 7 9
SpindlePole 49 232 247 163 254 200 310 278 157 – 232 209 164 234 154 109 130 70 73 258 234 149 270 178
Vac/Vac Membrane 0 5 0 0 1 0 0 2 0 – 0 0 0 4 1 6 6 4 0 4 3 1 8 11
Unique Cell Count 60 357 283 201 309 252 415 395 262 336 295 273 442 340 215 219 140 91 395 308 206 365 280
Labelled Cell Count 101 528 391 277 438 382 594 557 401 542 443 437 681 460 339 388 232 91 395 308 206 365 280


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.1 5.6 5.3 6.0 4.7 4.9 5.3 4.1 – 3.8 4.4 5.1 5.4 5.4 8.9 9.2 8.8 4.7 4.8 4.8
Std Deviation (1e-4) 1.3 1.0 1.1 1.4 1.5 1.2 1.3 1.8 1.3 – 1.2 1.3 1.3 1.3 1.7 1.9 2.5 1.9 1.3 1.7 1.5
Intensity Change (Log2) – – – -0.07 0.1 -0.26 -0.19 -0.07 -0.45 – -0.55 -0.35 -0.12 -0.06 -0.06 0.68 0.72 0.66 -0.24 -0.2 -0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 1.3 3.3 0 – 0 0 0 0 0 0 0 0
Bud Neck -1.0 -1.8 0 -0.1 0.7 0 – 0 0 0 0 0 0 0 0
Bud Site 0.7 -0.4 0 -0.4 -0.9 0 – 0 0 -1.0 -4.0 -2.2 -3.3 -3.3 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 1.2 0.8 1.5 1.2 3.9 2.3 – 1.5 1.7 1.2 3.0 3.6 2.5 1.9 2.1
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 4.6 7.5 4.1 6.3 8.0 8.3
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria -0.8 -1.8 0 2.9 1.6 0 – 0 0 2.2 -2.9 -2.0 -0.7 -1.3 -0.3
Nucleus 0.9 0.5 0.3 1.4 1.7 2.2 – 1.8 2.4 -0.3 2.8 4.0 3.7 4.1 2.3
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0.9 3.4 0 2.5 0.5 0 – 0 0 9.2 5.7 4.8 7.0 7.9 6.3
Peroxisomes 0 0 0 0 0 0 – 0 0 1.0 5.2 3.9 0 2.1 0
SpindlePole -1.9 -1.7 0 -4.1 -5.2 0 – 0 0 -7.3 -9.5 -10.9 -9.0 -7.2 -8.3
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8115 3.7413 3.373 2.7924 2.8384 2.585 0.5621 2.1828 1.7958 1.4923 1.4337 1.1088 -0.8091 0.4858 0.5091 0.7243 0.2324 -0.3977
Actin 0.0181 0 0.0166 0.0143 0.0002 0.0007 0.0127 0 0.0012 0.0001 0.0003 0.0044 0.0062 0 0.0002 0 0.0017 0.0002
Bud 0.0005 0 0.0002 0.0001 0 0 0.0001 0 0 0 0 0 0.0006 0.0001 0.0011 0 0.0001 0.0003
Bud Neck 0.0022 0.0003 0.0014 0.0002 0.0001 0.0045 0.001 0.0004 0.0002 0.0001 0.0002 0.0001 0.0006 0.0004 0.0009 0 0.0016 0.0069
Bud Periphery 0.0013 0 0.0005 0.0001 0 0.0001 0.0001 0 0.0001 0 0 0 0.0014 0.0003 0.0018 0 0.0003 0.0012
Bud Site 0.0046 0.0001 0.002 0.0005 0 0.0001 0.0019 0.0008 0.0002 0.0001 0.0001 0 0.0013 0.0013 0.0068 0 0.0005 0.0002
Cell Periphery 0.0004 0 0.0002 0 0 0 0.0001 0 0 0 0 0 0.0004 0.0002 0.0006 0 0.0003 0.0011
Cytoplasm 0.0017 0 0.0007 0.0001 0.0001 0.0004 0.0009 0 0.0039 0 0.0002 0 0.0053 0.0004 0.0007 0.0004 0.0004 0.001
Cytoplasmic Foci 0.0538 0.0022 0.0159 0.0138 0.0307 0.0058 0.0377 0.0067 0.0107 0.0195 0.0297 0.0027 0.0438 0.0588 0.0139 0.0387 0.0223 0.0331
Eisosomes 0.0012 0 0.0015 0.0001 0 0.0002 0.0002 0 0 0 0 0.0008 0.0033 0.0002 0.001 0 0.0004 0.0001
Endoplasmic Reticulum 0.0005 0 0.0006 0.0001 0 0.0002 0.0004 0 0.0006 0 0.0001 0 0.0004 0 0.0001 0 0.0006 0
Endosome 0.0063 0.0001 0.0057 0.0009 0.0005 0.0022 0.0052 0.0002 0.0071 0.0002 0.0047 0.0007 0.0037 0.0029 0.0002 0.0001 0.0061 0.0008
Golgi 0.0051 0.0001 0.0026 0.0013 0.0015 0.0007 0.0025 0.0003 0.002 0.0004 0.0035 0.0011 0.0013 0.0008 0.0002 0.0001 0.0058 0.0005
Lipid Particles 0.0331 0.0012 0.0148 0.0149 0.0323 0.0077 0.0228 0.0061 0.0183 0.0052 0.0469 0.0029 0.011 0.0194 0.0029 0.0034 0.1739 0.0151
Mitochondria 0.0128 0 0.0187 0.0067 0 0.0021 0.0011 0 0.0055 0 0.0008 0.0025 0.0017 0.0015 0.0011 0 0.0021 0.0006
None 0.0388 0 0.0087 0.0003 0.0001 0.0088 0.0069 0 0.0012 0 0.0002 0.0001 0.069 0.0961 0.0589 0.0024 0.0018 0.1069
Nuclear Periphery 0.0023 0.0002 0.0038 0.0006 0.0031 0.0019 0.0011 0.0024 0.0018 0.0009 0.0044 0.0021 0.0035 0.0002 0.0004 0.0002 0.0082 0.0005
Nucleolus 0.0124 0.0572 0.0223 0.0241 0.0041 0.0158 0.0145 0.036 0.0142 0.0028 0.0033 0.0064 0.0131 0.0496 0.0399 0.0019 0.037 0.0162
Nucleus 0.0166 0.0191 0.0045 0.0024 0.0005 0.0067 0.0053 0.0177 0.0043 0.0006 0.0008 0.0022 0.0229 0.0095 0.0171 0.0008 0.006 0.005
Peroxisomes 0.0136 0.0001 0.0063 0.023 0.0105 0.0019 0.0121 0.0016 0.0033 0.0005 0.0191 0.0015 0.0042 0.011 0.0018 0.0003 0.012 0.0126
Punctate Nuclear 0.7727 0.9193 0.8709 0.8963 0.9162 0.9397 0.8724 0.9276 0.925 0.9696 0.8855 0.9722 0.8055 0.7452 0.8503 0.9516 0.7174 0.7971
Vacuole 0.0013 0 0.0011 0 0 0.0002 0.0008 0 0.0003 0 0.0001 0 0.0006 0.0018 0.0003 0 0.001 0.0004
Vacuole Periphery 0.0006 0 0.001 0.0001 0 0.0003 0.0002 0 0.0002 0 0.0001 0 0.0002 0.0003 0 0 0.0007 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.9279 27.9993 6.233 12.0196 32.4875 32.1984 19.3115 12.5563 11.47 33.2088
Translational Efficiency 2.7733 1.7276 1.9458 1.6981 1.9715 2.6093 1.6548 1.3601 1.2989 1.6825

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Kinetochore-associated protein; required for chromosome segregation and kinetochore clustering; required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate
Localization
Cell Percentages spindle pole (77%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Slk19

Slk19


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Slk19-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available