Standard name
Human Ortholog
Description Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics and lifespan extension; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that block potential dimerization

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.96 0.78 0.83 0.85 0.71 0.67 0.67 0.73 0.71 0.66 0.93 0.98 0.93 0.91 0.95 0.92 0.94 0.89 0.96 0.88 0.79 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Mitochondria 0 0 0 0 0 0.08 0.16 0.11 0.15 0.16 0.18 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.24 0.14 0.08 0.18 0.24 0.21 0.13 0.18 0.22 0.09 0.05 0.11 0.13 0.07 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 1 0 0 0 1 0 0 3 4
Bud 0 0 0 0 0 0 6 4 8 4 6 11 0 0 0 0 0 0 1 2 0 4 8 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 3
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2 2 3 1 9 5 12 9 5 7 0 2 2 1 2 1 0 0 0 0 0 0
Cytoplasm 270 284 386 208 252 379 436 357 374 430 404 376 419 632 611 143 202 177 258 256 382 349 365 402
Endoplasmic Reticulum 1 2 5 2 4 11 15 9 9 17 3 8 2 6 3 3 4 5 3 0 1 1 4 6
Endosome 0 1 1 0 1 0 0 5 0 0 1 3 2 0 2 0 1 2 1 2 0 4 9 23
Golgi 0 1 0 2 1 0 0 1 0 0 0 0 1 0 0 1 1 1 0 2 2 0 6 28
Mitochondria 1 0 0 5 11 35 97 58 83 92 100 92 1 0 0 2 2 1 0 1 0 2 2 6
Nucleus 3 0 5 1 4 9 18 15 18 16 15 10 3 16 9 2 1 3 3 9 4 24 29 24
Nuclear Periphery 0 0 0 0 0 0 1 2 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 3 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1 5 9
Vac/Vac Membrane 1 5 18 64 44 36 110 129 115 76 105 122 39 33 72 21 15 16 0 6 3 4 16 17
Unique Cell Count 272 291 401 265 304 444 616 529 558 593 568 566 452 645 654 158 212 192 274 288 400 396 462 548
Labelled Cell Count 276 293 417 285 320 471 692 587 620 644 640 630 467 689 699 178 228 207 274 288 400 396 462 548


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.7 10.9 10.7 9.4 9.4 8.5 8.3 8.7 8.2 7.9 8.0 7.8 12.4 12.1 11.4 13.6 12.7 13.2 12.4 12.3 14.1
Std Deviation (1e-4) 1.8 2.4 1.9 1.7 1.8 1.8 1.8 1.9 1.8 1.7 1.8 1.8 2.3 2.0 2.0 3.6 2.9 3.0 2.5 2.5 2.8
Intensity Change (Log2) -0.18 -0.18 -0.33 -0.37 -0.3 -0.39 -0.44 -0.42 -0.45 0.21 0.17 0.09 0.34 0.25 0.3 0.22 0.2 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 2.1 0 0 0 0 0 0 0 0 0
Cytoplasm -7.2 -6.0 -5.4 -10.1 -10.8 -11.0 -9.6 -9.9 -11.2 -2.3 1.7 -2.0 -2.7 -0.6 -2.1
Endoplasmic Reticulum 0 0 1.3 1.3 0.6 0.5 1.7 0 0.2 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.7 8.4 6.8 8.1 8.3 8.9 8.5 0 0 0 0 0 0
Nucleus 0 0 0.9 1.8 1.7 2.0 1.6 1.5 0.6 0 1.4 0.2 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 7.6 4.6 2.1 6.3 8.2 7.1 4.4 6.4 7.4 2.4 0.5 3.7 3.7 1.3 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.1099 7.5801 7.7622 6.6917 6.8739 7.6029 4.4077 5.8866 5.4204 5.0324 4.9291 5.642 5.2444 6.0483 5.5234 5.3784 5.0271 5.5186
Actin 0.0292 0.0019 0.0093 0.0008 0.0359 0.0008 0.0454 0.0007 0.0134 0.0311 0.0044 0.0004 0.0094 0.0009 0.0108 0.0016 0.0289 0.0052
Bud 0.0009 0.0012 0.0008 0.0001 0.0003 0.0001 0.0009 0.0004 0.0023 0.0006 0.0002 0.0003 0.0011 0.0033 0.0007 0.0002 0.0019 0.0009
Bud Neck 0.009 0.0004 0.0008 0.0006 0.0005 0.0014 0.0134 0.0006 0.0131 0.0017 0.0005 0.0034 0.0065 0.0007 0.0014 0.0013 0.0067 0.0076
Bud Periphery 0.001 0.0004 0.0005 0 0.0002 0 0.0009 0.0002 0.0011 0.0005 0.0003 0.0001 0.001 0.001 0.0003 0.0001 0.001 0.0008
Bud Site 0.0033 0.0043 0.0051 0.0003 0.0004 0.0001 0.0118 0.0032 0.0081 0.0024 0.0002 0.0004 0.0059 0.0159 0.0057 0.0004 0.0021 0.0007
Cell Periphery 0.0002 0.0001 0.0001 0.0001 0 0 0.0007 0.0001 0.0004 0.0001 0.0001 0 0.0004 0.0001 0.0001 0 0.0001 0.0001
Cytoplasm 0.7426 0.906 0.8369 0.9554 0.8466 0.924 0.7667 0.9483 0.8811 0.7588 0.8412 0.9493 0.826 0.9014 0.867 0.83 0.794 0.8475
Cytoplasmic Foci 0.0235 0.0158 0.0251 0.0025 0.0171 0.006 0.0162 0.002 0.0061 0.0271 0.0054 0.0039 0.0155 0.0047 0.0065 0.0124 0.0141 0.0072
Eisosomes 0.0001 0 0.0001 0 0 0 0.0005 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0 0.0005 0
Endoplasmic Reticulum 0.0127 0.0029 0.0031 0.003 0.0037 0.0026 0.014 0.0033 0.0023 0.0025 0.0021 0.0024 0.0071 0.0021 0.0023 0.0028 0.0092 0.003
Endosome 0.0269 0.0038 0.0119 0.0012 0.0169 0.0019 0.0405 0.0006 0.0061 0.0492 0.0454 0.0024 0.0192 0.0042 0.0072 0.0027 0.0279 0.0059
Golgi 0.0054 0.0015 0.0036 0.0001 0.0035 0.0001 0.01 0.0001 0.0083 0.0318 0.0081 0.0001 0.0029 0.0002 0.0023 0.0003 0.009 0.0005
Lipid Particles 0.002 0.0002 0.0014 0 0.0144 0 0.004 0 0.0015 0.0031 0.0013 0 0.0052 0.0001 0.0007 0.0003 0.0107 0.0006
Mitochondria 0.0012 0.0003 0.0023 0.0002 0.0006 0.0002 0.0041 0.0001 0.0114 0.0361 0.0549 0.0002 0.0036 0.0004 0.001 0.0003 0.0023 0.0005
None 0.1117 0.038 0.0609 0.0144 0.0189 0.0366 0.028 0.0208 0.0082 0.0033 0.0193 0.0041 0.0186 0.0124 0.0144 0.0094 0.0121 0.0149
Nuclear Periphery 0.0125 0.0022 0.0028 0.0022 0.0061 0.002 0.0127 0.0015 0.0018 0.0032 0.0009 0.0023 0.0102 0.0046 0.0038 0.0062 0.017 0.0084
Nucleolus 0.0002 0.0001 0.0004 0 0.0001 0 0.001 0 0.0003 0.0001 0 0 0.0005 0.0003 0.0004 0.0002 0.0009 0.0005
Nucleus 0.0089 0.0152 0.0235 0.0169 0.0091 0.0217 0.015 0.0161 0.0308 0.0374 0.009 0.0281 0.0524 0.0408 0.0679 0.1185 0.0366 0.0789
Peroxisomes 0.0027 0.0022 0.0065 0.0001 0.0165 0.0003 0.0021 0.0001 0.0011 0.0069 0.0006 0.0001 0.0043 0.0001 0.0012 0.0006 0.0065 0.0004
Punctate Nuclear 0.0039 0.0027 0.0034 0.0016 0.0077 0.0011 0.0039 0.0013 0.001 0.0022 0.0004 0.0017 0.0063 0.0054 0.005 0.0116 0.0146 0.0151
Vacuole 0.0018 0.0007 0.0012 0.0005 0.001 0.0007 0.0062 0.0004 0.0009 0.0013 0.0035 0.0009 0.0028 0.0012 0.0009 0.0009 0.0026 0.0011
Vacuole Periphery 0.0005 0.0001 0.0003 0.0001 0.0004 0.0001 0.0022 0.0001 0.0004 0.0006 0.0019 0.0001 0.001 0.0002 0.0002 0.0001 0.0011 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 85.3673 66.8215 58.0363 91.8814 81.4975 72.1612 73.8972 89.6466 76.6839 98.2224
Translational Efficiency 1.5431 1.541 1.7262 0.8616 1.2493 1.8303 1.3542 0.8073 1.1994 1.1271

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics and lifespan extension; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that block potential dimerization
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mca1

Mca1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mca1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available