Standard name | |
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Human Ortholog | |
Description | Ribose methyltransferase; modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 1 | 3 | 3 | 3 | 1 | 3 | 7 | 3 | 3 | 10 | 9 |
Bud | 7 | 9 | 21 | 15 | 10 | 14 | 24 | 21 | 20 | 16 | 26 | 25 | 9 | 5 | 9 | 9 | 10 | 6 | 0 | 0 | 1 | 2 | 1 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 2 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 2 | 4 |
Bud Site | 0 | 0 | 1 | 2 | 0 | 5 | 10 | 14 | 8 | 10 | 14 | 6 | 0 | 0 | 1 | 2 | 3 | 3 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 11 | 4 | 5 | 1 | 3 | 2 | 8 | 5 | 6 | 4 | 7 | 5 | 2 | 7 | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 6 | 14 | 9 | 11 | 11 | 16 | 10 | 14 | 9 | 5 | 11 | 52 | 83 | 142 | 18 | 16 | 18 | 0 | 0 | 0 | 4 | 4 | 5 |
Endoplasmic Reticulum | 2 | 2 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 5 | 0 | 11 | 9 | 0 | 6 | 5 | 12 | 5 | 0 | 1 | 0 | 1 | 0 | 3 |
Endosome | 4 | 6 | 56 | 9 | 38 | 2 | 2 | 8 | 6 | 2 | 2 | 2 | 70 | 106 | 72 | 35 | 49 | 27 | 34 | 31 | 35 | 37 | 52 | 46 |
Golgi | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 5 | 6 | 5 | 10 | 8 | 11 | 10 | 16 | 25 |
Mitochondria | 208 | 134 | 205 | 258 | 202 | 355 | 485 | 468 | 454 | 506 | 385 | 498 | 56 | 21 | 21 | 27 | 27 | 20 | 146 | 98 | 197 | 230 | 233 | 229 |
Nucleus | 0 | 1 | 0 | 0 | 2 | 4 | 12 | 3 | 5 | 4 | 3 | 1 | 3 | 0 | 1 | 7 | 1 | 4 | 0 | 0 | 0 | 2 | 0 | 2 |
Nuclear Periphery | 3 | 0 | 0 | 1 | 0 | 16 | 14 | 14 | 9 | 14 | 5 | 30 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 3 |
Nucleolus | 1 | 1 | 0 | 0 | 5 | 13 | 18 | 16 | 7 | 9 | 10 | 9 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 2 | 1 | 1 | 4 |
Peroxisomes | 4 | 8 | 108 | 44 | 37 | 16 | 8 | 12 | 12 | 1 | 6 | 8 | 22 | 92 | 60 | 22 | 40 | 12 | 26 | 9 | 45 | 21 | 25 | 40 |
SpindlePole | 9 | 7 | 56 | 14 | 20 | 18 | 21 | 30 | 30 | 9 | 10 | 13 | 81 | 95 | 64 | 48 | 52 | 22 | 2 | 3 | 9 | 10 | 14 | 10 |
Vac/Vac Membrane | 22 | 13 | 6 | 3 | 23 | 17 | 18 | 20 | 19 | 22 | 21 | 24 | 28 | 48 | 22 | 14 | 24 | 18 | 2 | 6 | 1 | 14 | 15 | 11 |
Unique Cell Count | 226 | 156 | 304 | 294 | 262 | 383 | 519 | 509 | 484 | 520 | 396 | 517 | 283 | 332 | 327 | 162 | 186 | 122 | 231 | 171 | 317 | 350 | 387 | 410 |
Labelled Cell Count | 270 | 192 | 483 | 361 | 354 | 473 | 633 | 619 | 593 | 612 | 493 | 643 | 358 | 457 | 407 | 204 | 253 | 150 | 231 | 171 | 317 | 350 | 387 | 410 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 4.4 | 4.6 | 4.6 | 4.9 | 3.9 | 3.7 | 3.9 | 3.8 | 3.6 | 3.5 | 3.6 | 5.9 | 5.9 | 5.5 | 6.3 | 5.8 | 5.6 | 6.3 | 6.4 | 7.3 |
Std Deviation (1e-4) | 0.6 | 0.6 | 1.2 | 1.3 | 1.8 | 1.3 | 1.3 | 1.1 | 0.9 | 0.9 | 0.8 | 0.8 | 1.1 | 1.5 | 0.9 | 1.6 | 1.3 | 1.5 | 1.2 | 1.2 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.11 | -0.23 | -0.28 | -0.24 | -0.27 | -0.34 | -0.36 | -0.35 | 0.38 | 0.37 | 0.27 | 0.46 | 0.36 | 0.29 | 0.47 | 0.48 | 0.69 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.4 | 4.9 | 5.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.4609 | 6.011 | 6.0397 | 5.3424 | 5.4237 | 6.0483 | 5.1444 | 5.6968 | 5.3933 | 5.7684 | 4.9625 | 5.2961 | 4.6905 | 5.1346 | 4.7286 | 4.9797 | 4.4128 | 4.7509 |
Actin | 0.001 | 0.0007 | 0.0013 | 0.0004 | 0.0002 | 0.0004 | 0.0043 | 0.0029 | 0.0011 | 0.0005 | 0.0025 | 0.003 | 0.0049 | 0.002 | 0.0059 | 0.0001 | 0.0034 | 0.0021 |
Bud | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0033 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Bud Periphery | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0005 | 0.0004 | 0.0001 | 0.0006 | 0.0002 | 0.0011 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0.0009 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0011 | 0.0008 | 0.0003 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0011 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0026 | 0.0016 | 0.0009 | 0.0003 | 0.0004 | 0.0008 | 0.0038 | 0.0043 | 0.0047 | 0.0011 | 0.002 | 0.0021 | 0.0035 | 0.0035 | 0.002 | 0.0001 | 0.0091 | 0.0035 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 |
Endosome | 0.0072 | 0.0028 | 0.0027 | 0.001 | 0.0017 | 0.0022 | 0.0177 | 0.0139 | 0.0173 | 0.0033 | 0.0031 | 0.0094 | 0.0197 | 0.0149 | 0.016 | 0.0004 | 0.0133 | 0.0031 |
Golgi | 0.0222 | 0.0131 | 0.0061 | 0.0027 | 0.0051 | 0.0158 | 0.0261 | 0.0382 | 0.0216 | 0.0067 | 0.0086 | 0.0272 | 0.0216 | 0.0337 | 0.0284 | 0.0007 | 0.0449 | 0.0179 |
Lipid Particles | 0.005 | 0.0071 | 0.0038 | 0.0007 | 0.0006 | 0.0029 | 0.0201 | 0.0017 | 0.002 | 0.0006 | 0.0028 | 0.0045 | 0.0138 | 0.0096 | 0.0017 | 0.0002 | 0.011 | 0.0067 |
Mitochondria | 0.9403 | 0.9364 | 0.9735 | 0.9904 | 0.9893 | 0.9693 | 0.9049 | 0.9238 | 0.9412 | 0.9842 | 0.9727 | 0.9433 | 0.9019 | 0.902 | 0.9298 | 0.9948 | 0.8953 | 0.959 |
None | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0007 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0.0015 | 0 | 0.0005 | 0.0002 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.0199 | 0.0374 | 0.0094 | 0.0036 | 0.002 | 0.0079 | 0.0155 | 0.0077 | 0.0076 | 0.0027 | 0.0024 | 0.0056 | 0.0221 | 0.0123 | 0.0096 | 0.0031 | 0.021 | 0.0061 |
Punctate Nuclear | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0017 | 0.0015 | 0.0008 | 0.0002 | 0.0009 | 0.0005 | 0.0021 | 0.0085 | 0.0026 | 0.0001 | 0.0003 | 0.0002 |
Vacuole Periphery | 0.0008 | 0.0005 | 0.001 | 0.0003 | 0.0003 | 0.0003 | 0.0025 | 0.0032 | 0.0014 | 0.0003 | 0.0014 | 0.0019 | 0.0031 | 0.0107 | 0.0028 | 0.0003 | 0.001 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 28.0527 | 28.6598 | 18.3131 | 21.0824 | 27.7116 | 18.6914 | 29.5816 | 21.7313 | 30.0604 | 28.6921 |
Translational Efficiency | 0.8811 | 0.6563 | 0.8939 | 0.787 | 0.6762 | 1.3527 | 0.9328 | 1.0523 | 0.7814 | 0.9088 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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