| Standard name | |
|---|---|
| Human Ortholog | |
| Description | G protein beta subunit; forms a dimer with Ste18p to activate mating signaling pathway, forms heterotrimer with Gpa1p and Ste18p to dampen signaling; pheromone-induced phosphorylation plays critical role in chemotropism; may recruit Rho1p to polarized growth site during mating; contains WD40 repeats |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | – | – | 2 | 0 | 0 | 3 | 2 | 1 | 0 | 1 | 2 | 2 | – | – | – | – | 0 | – | 0 | – | 7 | 0 | 0 | 0 |
| Bud | – | – | 2 | 1 | 2 | 1 | 2 | 9 | 1 | 3 | 3 | 6 | – | – | – | – | 0 | – | 0 | – | 2 | 0 | 0 | 1 |
| Bud Neck | – | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | – | – | – | – | 3 | – | 0 | – | 4 | 0 | 0 | 0 |
| Bud Site | – | – | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | 0 | – | – | – | – | – | – | – |
| Cell Periphery | – | – | 25 | 12 | 5 | 8 | 18 | 10 | 5 | 11 | 7 | 17 | – | – | – | – | 1 | – | 0 | – | 25 | 0 | 0 | 0 |
| Cytoplasm | – | – | 88 | 48 | 34 | 68 | 76 | 118 | 19 | 57 | 35 | 97 | – | – | – | – | 36 | – | 2 | – | 44 | 1 | 2 | 1 |
| Endoplasmic Reticulum | – | – | 3 | 0 | 0 | 3 | 0 | 0 | 2 | 0 | 1 | 0 | – | – | – | – | 3 | – | 0 | – | 13 | 0 | 0 | 0 |
| Endosome | – | – | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | – | – | – | – | 0 | – | 0 | – | 1 | 1 | 0 | 0 |
| Golgi | – | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | 0 | – | 0 | – | 1 | 0 | 0 | 0 |
| Mitochondria | – | – | 4 | 2 | 1 | 7 | 8 | 33 | 16 | 30 | 26 | 69 | – | – | – | – | 2 | – | 0 | – | 1 | 0 | 0 | 0 |
| Nucleus | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | 0 | – | 0 | – | 0 | 0 | 0 | 0 |
| Nuclear Periphery | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | 0 | – | 0 | – | 0 | 0 | 0 | 0 |
| Nucleolus | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | – | – | – | 0 | – | 0 | – | 0 | 0 | 0 | 0 |
| Peroxisomes | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | 0 | – | 0 | – | 0 | 0 | 0 | 0 |
| SpindlePole | – | – | 0 | 0 | 0 | 2 | 4 | 1 | 0 | 1 | 1 | 0 | – | – | – | – | 4 | – | 0 | – | 0 | 0 | 0 | 0 |
| Vac/Vac Membrane | – | – | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | – | – | – | – | 1 | – | 0 | – | 0 | 0 | 0 | 0 |
| Unique Cell Count | 95 | 53 | 35 | 83 | 87 | 147 | 34 | 73 | 56 | 150 | 41 | 3 | 108 | 4 | 4 | 5 | ||||||||
| Labelled Cell Count | 125 | 63 | 42 | 96 | 114 | 177 | 45 | 107 | 75 | 194 | 50 | 3 | 108 | 4 | 4 | 5 | ||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | – | – | 6.0 | 5.4 | 5.1 | 5.2 | 4.8 | 4.6 | 4.2 | 3.8 | 4.3 | 4.0 | – | – | – | – | 6.5 | – | – | – | – |
| Std Deviation (1e-4) | – | – | 1.7 | 1.0 | 1.8 | 2.3 | 1.5 | 1.2 | 1.1 | 0.6 | 0.9 | 0.6 | – | – | – | – | 2.4 | – | – | – | – |
| Intensity Change (Log2) | – | – | – | -0.14 | -0.25 | -0.21 | -0.34 | -0.38 | -0.51 | -0.64 | -0.49 | -0.59 | – | – | – | – | 0.11 | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | 0 | – |
| Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | 0 | – |
| Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | 0 | – |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | 0 | – |
| Cell Periphery | -0.5 | -1.4 | -2.9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | -3.2 | – |
| Cytoplasm | -0.4 | 0.9 | -2.2 | -1.2 | -2.6 | -4.9 | -2.7 | -4.6 | -5.0 | – | – | – | – | -0.9 | – |
| Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | 0 | – |
| Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | 0 | – |
| Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | 0 | – |
| Mitochondria | 0 | 0 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | 0 | – |
| Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | 0 | – |
| Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | 0 | – |
| Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | 0 | – |
| Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | 0 | – |
| SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | 0 | – |
| Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | 0 | – |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Actin | 0.184 | 0.007 | 0.0288 | 0.0702 | 0.2116 | 0.0091 | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | 0.0051 | 0.0005 | 0.0092 | 0.004 | 0.0017 | 0.0005 | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | 0.0055 | 0.0013 | 0.0013 | 0.0117 | 0.0015 | 0.0359 | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Periphery | 0.0056 | 0.0014 | 0.0052 | 0.0121 | 0.0024 | 0.0035 | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | 0.0135 | 0.027 | 0.0141 | 0.0094 | 0.0159 | 0.0005 | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | 0.0226 | 0.0524 | 0.0135 | 0.0202 | 0.0012 | 0.0189 | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | 0.2638 | 0.835 | 0.7342 | 0.717 | 0.4998 | 0.8362 | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasmic Foci | 0.074 | 0.0023 | 0.0068 | 0.0376 | 0.0556 | 0.0061 | – | – | – | – | – | – | – | – | – | – | – | – |
| Eisosomes | 0.0012 | 0.0006 | 0.0004 | 0.0001 | 0.0014 | 0.0001 | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | 0.0215 | 0.0054 | 0.0076 | 0.0081 | 0.0016 | 0.0069 | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | 0.0658 | 0.0014 | 0.0168 | 0.0541 | 0.0212 | 0.0304 | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | 0.0377 | 0.0017 | 0.012 | 0.0381 | 0.0297 | 0.0057 | – | – | – | – | – | – | – | – | – | – | – | – |
| Lipid Particles | 0.0948 | 0.0011 | 0.0044 | 0.0063 | 0.0327 | 0.0013 | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | 0.0567 | 0.0196 | 0.057 | 0.0011 | 0.0391 | 0.0011 | – | – | – | – | – | – | – | – | – | – | – | – |
| None | 0.0573 | 0.0389 | 0.0718 | 0.004 | 0.0054 | 0.0221 | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | 0.0052 | 0.0004 | 0.0018 | 0.0005 | 0.0005 | 0.0059 | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | 0.0049 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | 0.0033 | 0.0006 | 0.0015 | 0.0003 | 0.0004 | 0.0119 | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | 0.0428 | 0.0012 | 0.01 | 0.0015 | 0.0747 | 0.0003 | – | – | – | – | – | – | – | – | – | – | – | – |
| Punctate Nuclear | 0.0255 | 0.0001 | 0.0005 | 0.0005 | 0.0017 | 0.0016 | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | 0.0072 | 0.0018 | 0.0025 | 0.0028 | 0.0009 | 0.0015 | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole Periphery | 0.002 | 0.0001 | 0.0005 | 0.0004 | 0.0005 | 0.0004 | – | – | – | – | – | – | – | – | – | – | – | – |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 42.5054 | 44.2008 | 35.1006 | 59.7748 | 57.9218 | 19.9405 | 35.6747 | 38.3661 | 34.8052 | 59.2159 |
| Translational Efficiency | 1.1023 | 0.9069 | 1.3206 | 0.7453 | 0.9508 | 2.0052 | 0.987 | 0.7559 | 0.8806 | 0.6555 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | – |
| Protein Localization | ✘ |
| Gene Expression | ✘ |
| Translational Efficiency | ✘ |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description | G protein beta subunit; forms a dimer with Ste18p to activate mating signaling pathway, forms heterotrimer with Gpa1p and Ste18p to dampen signaling; pheromone-induced phosphorylation plays critical role in chemotropism; may recruit Rho1p to polarized growth site during mating; contains WD40 repeats |
| Localization | |
| Cell Percentages | cytoplasm (48%) |
| Cell Cycle Regulation | No |
| Subcompartmental Group | cytoplasm-10 |
Ste4
Ste4
Nearest neighbours ( genes)
correlation threshold
0.80
Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Ste4-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
