Standard name | |
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Human Ortholog | |
Description | RNA polymerase subunit ABC14.5; common to RNA polymerases I, II, and III |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 2 | 1 | 1 | 3 |
Bud Neck | 0 | 1 | 0 | 0 | 1 | 0 | – | 1 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 0 | 1 | 0 | 2 | – | 8 | 4 | 7 | 8 | 9 | 0 | 0 | 1 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 7 | 11 | 10 | 12 | 6 | – | 11 | 5 | 8 | 2 | 2 | 17 | 28 | 36 | 27 | 11 | 16 | 0 | 1 | 1 | 5 | 2 | 2 |
Endoplasmic Reticulum | 0 | 0 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 4 | 1 | – | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 8 | 3 | 2 | 2 | 1 | 0 | 6 | 0 | 1 | 4 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 2 | 3 |
Mitochondria | 5 | 4 | 17 | 6 | 7 | 8 | – | 20 | 20 | 27 | 33 | 36 | 0 | 1 | 0 | 7 | 9 | 4 | 0 | 1 | 2 | 0 | 1 | 2 |
Nucleus | 219 | 178 | 212 | 177 | 166 | 210 | – | 286 | 162 | 208 | 177 | 251 | 230 | 206 | 223 | 161 | 104 | 145 | 210 | 167 | 182 | 88 | 126 | 130 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2 | – | 3 | 4 | 2 | 0 | 4 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 113 | 95 | 155 | 97 | 88 | 146 | – | 266 | 171 | 254 | 213 | 237 | 149 | 102 | 105 | 55 | 39 | 50 | 30 | 44 | 87 | 57 | 93 | 116 |
Peroxisomes | 1 | 1 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 10 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 3 |
Vac/Vac Membrane | 0 | 3 | 0 | 0 | 2 | 1 | – | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 4 | 3 | 3 | 5 | 5 | 4 | 5 | 7 | 8 | 19 |
Unique Cell Count | 250 | 214 | 281 | 211 | 222 | 275 | 422 | 252 | 357 | 305 | 364 | 292 | 261 | 315 | 222 | 141 | 192 | 254 | 224 | 298 | 165 | 241 | 292 | |
Labelled Cell Count | 342 | 289 | 397 | 292 | 281 | 377 | 597 | 367 | 511 | 436 | 542 | 402 | 347 | 392 | 258 | 171 | 227 | 254 | 224 | 298 | 165 | 241 | 292 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.3 | 22.3 | 12.3 | 15.6 | 14.8 | 12.3 | – | 11.3 | 10.5 | 10.8 | 10.5 | 10.6 | 17.4 | 18.6 | 16.9 | 17.4 | 17.6 | 16.4 | 20.6 | 19.7 | 19.6 |
Std Deviation (1e-4) | 4.5 | 7.6 | 4.2 | 4.9 | 5.3 | 3.4 | – | 3.4 | 3.7 | 3.1 | 3.0 | 3.5 | 6.0 | 5.8 | 6.1 | 6.4 | 6.0 | 5.8 | 11.4 | 6.1 | 5.4 |
Intensity Change (Log2) | – | – | – | 0.34 | 0.27 | 0.0 | – | -0.12 | -0.22 | -0.18 | -0.23 | -0.22 | 0.5 | 0.6 | 0.46 | 0.5 | 0.52 | 0.42 | 0.74 | 0.68 | 0.67 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.4 | 0.8 | -1.2 | – | -1.0 | -1.3 | -1.2 | -2.7 | -3.0 | 1.1 | 3.1 | 3.4 | 3.5 | 1.7 | 2.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.7 | -1.5 | -1.8 | – | -0.8 | 0.9 | 0.7 | 2.1 | 1.8 | -4.3 | -3.7 | -4.4 | -1.5 | 0.1 | -2.1 |
Nucleus | 2.3 | -0.2 | 0.3 | – | -2.2 | -2.8 | -4.5 | -4.5 | -1.8 | 0.9 | 1.0 | -1.3 | -0.7 | -0.4 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -2.0 | -3.5 | -0.5 | – | 2.1 | 3.0 | 4.2 | 3.7 | 2.6 | -1.0 | -3.7 | -5.4 | -6.9 | -5.3 | -6.3 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 12.1808 | 13.6742 | 12.497 | 11.6484 | 10.8139 | 12.1314 | 11.1813 | 11.0375 | 10.5618 | 10.372 | 8.7697 | 10.3675 | 14.9136 | 14.9744 | 15.0087 | 14.9088 | 13.5699 | 13.7176 |
Actin | 0.0276 | 0 | 0.0126 | 0.0324 | 0.0013 | 0.0007 | 0.0055 | 0 | 0.0073 | 0 | 0.0128 | 0.0002 | 0.0059 | 0 | 0.0128 | 0 | 0 | 0.0002 |
Bud | 0.0012 | 0 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0009 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0.0014 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0004 | 0 | 0.0041 | 0 | 0.0004 | 0.0004 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 |
Bud Periphery | 0.0015 | 0 | 0.0003 | 0.0002 | 0.0005 | 0.0001 | 0.0014 | 0 | 0.0002 | 0 | 0.0012 | 0.0001 | 0.0005 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Bud Site | 0.0043 | 0 | 0.0021 | 0.0003 | 0.0013 | 0.0002 | 0.0028 | 0 | 0.0016 | 0 | 0.001 | 0.0003 | 0.0006 | 0 | 0.0002 | 0 | 0 | 0 |
Cell Periphery | 0.0004 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0013 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0039 | 0.0001 | 0.0021 | 0.0001 | 0.0005 | 0.0001 | 0.0047 | 0 | 0.0002 | 0 | 0.002 | 0.0002 | 0.0012 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasmic Foci | 0.0116 | 0 | 0.0079 | 0.0027 | 0.0271 | 0.0016 | 0.0174 | 0 | 0.0078 | 0 | 0.0142 | 0.0016 | 0.0003 | 0 | 0.0003 | 0 | 0 | 0.0001 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0009 | 0 | 0.0011 | 0.0001 | 0.0002 | 0.0002 | 0.0027 | 0 | 0.0001 | 0 | 0.0007 | 0.0001 | 0.0018 | 0 | 0.0003 | 0 | 0 | 0.0002 |
Endosome | 0.0131 | 0 | 0.012 | 0.0019 | 0.0043 | 0.0058 | 0.0227 | 0 | 0.0033 | 0 | 0.0114 | 0.0036 | 0.0026 | 0 | 0.0012 | 0 | 0 | 0.0015 |
Golgi | 0.0072 | 0 | 0.0026 | 0.0045 | 0.0025 | 0.0021 | 0.0064 | 0 | 0.0025 | 0 | 0.0096 | 0.0004 | 0.0009 | 0 | 0.0007 | 0 | 0 | 0.0001 |
Lipid Particles | 0.0107 | 0 | 0.0117 | 0.0032 | 0.0242 | 0.0043 | 0.0216 | 0 | 0.0096 | 0 | 0.0349 | 0.0015 | 0.0009 | 0 | 0.0003 | 0 | 0 | 0.0001 |
Mitochondria | 0.0058 | 0.0002 | 0.0126 | 0.0081 | 0.012 | 0.0131 | 0.0096 | 0.0002 | 0.0016 | 0 | 0.0299 | 0.0015 | 0.0018 | 0.0001 | 0.0007 | 0 | 0.0009 | 0.0003 |
None | 0.0049 | 0 | 0.001 | 0.0002 | 0.0012 | 0.0001 | 0.0029 | 0 | 0.0003 | 0 | 0.004 | 0.0002 | 0.0005 | 0 | 0.0004 | 0 | 0 | 0.0001 |
Nuclear Periphery | 0.0151 | 0.0004 | 0.0038 | 0.0004 | 0.0018 | 0.0002 | 0.0115 | 0.0025 | 0.0009 | 0.0002 | 0.0024 | 0.0008 | 0.0068 | 0.0002 | 0.0007 | 0 | 0.0002 | 0.002 |
Nucleolus | 0.1245 | 0.0473 | 0.0503 | 0.0694 | 0.4483 | 0.2422 | 0.1076 | 0.0638 | 0.0457 | 0.0496 | 0.4002 | 0.2523 | 0.1233 | 0.0649 | 0.0401 | 0.0497 | 0.3604 | 0.2551 |
Nucleus | 0.7408 | 0.9516 | 0.8725 | 0.8629 | 0.4057 | 0.7259 | 0.7612 | 0.9323 | 0.901 | 0.9501 | 0.4378 | 0.7328 | 0.8474 | 0.9345 | 0.9409 | 0.9501 | 0.6377 | 0.7392 |
Peroxisomes | 0.0077 | 0 | 0.0035 | 0.0115 | 0.0113 | 0.0017 | 0.0043 | 0 | 0.0099 | 0 | 0.01 | 0.0002 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 |
Punctate Nuclear | 0.0126 | 0.0001 | 0.002 | 0.0014 | 0.0524 | 0.0004 | 0.0095 | 0.0001 | 0.0027 | 0.0001 | 0.0045 | 0.0009 | 0.0007 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0004 |
Vacuole | 0.0027 | 0.0001 | 0.0006 | 0.0001 | 0.0016 | 0.0004 | 0.0037 | 0.0001 | 0.0004 | 0 | 0.0093 | 0.0019 | 0.0023 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 |
Vacuole Periphery | 0.0018 | 0.0001 | 0.0009 | 0.0003 | 0.0027 | 0.0004 | 0.0026 | 0.0008 | 0.0004 | 0 | 0.0121 | 0.0007 | 0.0013 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 183.9007 | 219.1947 | 154.3534 | 170.8383 | 191.3971 | 117.5313 | 158.8708 | 152.6364 | 135.8458 | 160.4239 |
Translational Efficiency | 1.3882 | 1.2286 | 1.6738 | 1.3091 | 1.212 | 2.0386 | 1.5167 | 1.284 | 1.5676 | 1.5217 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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