Standard name | |
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Human Ortholog | |
Description | Transcriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats; relocalizes to the cytosol in response to hypoxia; WTM2 has a paralog, UME1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 3 | 4 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 2 | 1 | 3 | 8 | 6 | 12 | 29 | 12 | 10 | 9 | 6 | 33 | 42 | 54 | 5 | 14 | 6 | 4 | 1 | 0 | 0 | 2 | 2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 5 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 5 | 10 | 10 | 19 | 36 | 63 | 83 | 58 | 77 | 37 | 53 | 2 | 2 | 2 | 1 | 3 | 2 | 0 | 0 | 0 | 1 | 1 | 3 |
Nucleus | 78 | 258 | 97 | 72 | 96 | 103 | 137 | 184 | 132 | 147 | 70 | 118 | 243 | 287 | 341 | 151 | 114 | 96 | 103 | 257 | 93 | 259 | 249 | 195 |
Nuclear Periphery | 8 | 5 | 1 | 5 | 2 | 2 | 2 | 6 | 1 | 6 | 3 | 4 | 3 | 0 | 7 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 1 | 3 | 5 | 6 | 6 | 12 | 16 | 33 | 24 | 32 | 19 | 24 | 6 | 10 | 17 | 22 | 4 | 9 | 1 | 3 | 1 | 8 | 17 | 15 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 10 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 1 | 7 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 |
Vac/Vac Membrane | 1 | 5 | 0 | 1 | 8 | 2 | 4 | 8 | 2 | 1 | 1 | 6 | 4 | 3 | 13 | 8 | 10 | 4 | 1 | 9 | 3 | 13 | 20 | 37 |
Unique Cell Count | 82 | 270 | 101 | 80 | 117 | 124 | 184 | 267 | 175 | 200 | 105 | 152 | 288 | 345 | 430 | 182 | 142 | 115 | 118 | 279 | 100 | 290 | 300 | 267 |
Labelled Cell Count | 90 | 280 | 115 | 97 | 140 | 162 | 236 | 348 | 234 | 276 | 143 | 217 | 299 | 353 | 456 | 192 | 149 | 119 | 118 | 279 | 100 | 290 | 300 | 267 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.5 | 8.0 | 6.1 | 5.9 | 6.1 | 5.5 | 5.3 | 5.5 | 5.6 | 5.1 | 5.3 | 5.2 | 9.2 | 8.5 | 8.3 | 12.9 | 16.5 | 18.0 | 8.1 | 8.5 | 9.7 |
Std Deviation (1e-4) | 1.6 | 1.6 | 1.3 | 1.3 | 1.8 | 1.2 | 1.6 | 1.4 | 1.6 | 1.6 | 1.8 | 1.5 | 3.0 | 2.5 | 2.5 | 3.5 | 5.3 | 5.0 | 1.7 | 2.3 | 2.4 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.01 | -0.15 | -0.21 | -0.16 | -0.12 | -0.27 | -0.19 | -0.24 | 0.59 | 0.49 | 0.45 | 1.09 | 1.44 | 1.56 | 0.41 | 0.48 | 0.67 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 3.2 | 3.3 | 3.4 | 0 | 2.8 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.6 | 1.4 | 3.5 | 4.5 | 4.2 | 4.3 | 5.2 | 4.3 | 4.5 | 0 | 0 | 0 | 0 | -2.7 | -2.6 |
Nucleus | -1.6 | -3.2 | -3.1 | -4.5 | -5.5 | -4.4 | -4.7 | -5.4 | -4.0 | -3.0 | -3.3 | -4.0 | -3.2 | -3.6 | -3.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0.1 | 1.3 | 1.2 | 2.1 | 2.3 | 2.8 | 2.9 | 2.7 | 0 | 0 | 0 | 2.0 | 0 | 0.9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.3637 | 5.1314 | 4.648 | 4.6768 | 4.2464 | 4.8547 | 4.9703 | 5.7233 | 4.4403 | 3.4224 | 4.2461 | 4.6498 | 7.11 | 7.1854 | 6.8808 | 6.7369 | 5.9056 | 6.8561 |
Actin | 0.0112 | 0 | 0.0013 | 0 | 0.0001 | 0.0051 | 0.0019 | 0.0002 | 0.0175 | 0 | 0.0001 | 0.0072 | 0.009 | 0.0001 | 0.0157 | 0.023 | 0.009 | 0.0031 |
Bud | 0.0003 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0007 | 0.0001 |
Bud Neck | 0.0014 | 0.0002 | 0.0001 | 0.0001 | 0.0006 | 0.0008 | 0.0017 | 0.0001 | 0.0001 | 0 | 0.0006 | 0.0008 | 0.0016 | 0.0001 | 0.0004 | 0.0007 | 0.001 | 0.0004 |
Bud Periphery | 0.0007 | 0 | 0 | 0 | 0.0001 | 0.0005 | 0.0014 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0006 | 0.0003 | 0.0015 | 0.0006 |
Bud Site | 0.0011 | 0.0015 | 0.0005 | 0 | 0 | 0.0002 | 0.0009 | 0.0001 | 0.0006 | 0 | 0.0004 | 0.0002 | 0.0018 | 0.0001 | 0.006 | 0.0003 | 0.0034 | 0.0003 |
Cell Periphery | 0.0004 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0006 | 0.0001 |
Cytoplasm | 0.0088 | 0.0015 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0246 | 0.0014 | 0.0005 | 0 | 0.0006 | 0.0037 | 0.0014 | 0.0026 | 0.0052 | 0.0001 | 0.0004 | 0.0004 |
Cytoplasmic Foci | 0.0031 | 0 | 0.0004 | 0 | 0 | 0.0008 | 0.0078 | 0 | 0.0065 | 0 | 0.0001 | 0.0061 | 0.0075 | 0 | 0.0111 | 0.0008 | 0.0119 | 0.0031 |
Eisosomes | 0.0009 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.003 | 0 | 0.0015 | 0 | 0.0001 | 0.0002 | 0.0096 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0031 | 0.0009 | 0 | 0.0006 | 0.0002 | 0.0003 | 0.0008 |
Endosome | 0.0153 | 0 | 0.0026 | 0 | 0.0002 | 0.0018 | 0.0059 | 0.0002 | 0.0008 | 0 | 0.0002 | 0.0128 | 0.0134 | 0 | 0.0049 | 0.0009 | 0.011 | 0.0032 |
Golgi | 0.0075 | 0 | 0.0006 | 0 | 0 | 0.0013 | 0.0007 | 0 | 0.0009 | 0 | 0 | 0.0042 | 0.0043 | 0 | 0.0032 | 0.0016 | 0.0119 | 0.0011 |
Lipid Particles | 0.0087 | 0 | 0.0012 | 0 | 0 | 0.0025 | 0.0069 | 0 | 0.0046 | 0 | 0 | 0.0021 | 0.0094 | 0 | 0.0045 | 0.0007 | 0.0306 | 0.0023 |
Mitochondria | 0.0155 | 0.0001 | 0.0079 | 0.0001 | 0.0005 | 0.0016 | 0.0018 | 0.0002 | 0.0251 | 0 | 0.0005 | 0.0013 | 0.0156 | 0.0001 | 0.0096 | 0.0204 | 0.0121 | 0.0086 |
None | 0.0023 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.049 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0013 | 0 | 0.0021 | 0.0001 | 0.0003 | 0.0004 |
Nuclear Periphery | 0.0153 | 0.0044 | 0.0025 | 0.0037 | 0.0099 | 0.0035 | 0.0095 | 0.0107 | 0.0051 | 0.0004 | 0.0015 | 0.002 | 0.0176 | 0.0025 | 0.0116 | 0.0029 | 0.0008 | 0.0141 |
Nucleolus | 0.0138 | 0.0101 | 0.0059 | 0.0143 | 0.052 | 0.0436 | 0.0126 | 0.0057 | 0.0038 | 0.0018 | 0.0531 | 0.0216 | 0.0128 | 0.0055 | 0.0064 | 0.0042 | 0.033 | 0.0199 |
Nucleus | 0.8766 | 0.9805 | 0.9707 | 0.9813 | 0.9337 | 0.9277 | 0.8549 | 0.9796 | 0.9201 | 0.9976 | 0.9397 | 0.926 | 0.861 | 0.985 | 0.9015 | 0.9405 | 0.8335 | 0.9325 |
Peroxisomes | 0.0012 | 0 | 0.0001 | 0 | 0 | 0.0016 | 0.001 | 0 | 0.0105 | 0 | 0 | 0.0068 | 0.004 | 0 | 0.0114 | 0.0006 | 0.0311 | 0.0029 |
Punctate Nuclear | 0.0066 | 0.0012 | 0.0025 | 0.0003 | 0.0014 | 0.0066 | 0.006 | 0.0006 | 0.0027 | 0.0001 | 0.0017 | 0.001 | 0.0297 | 0.0036 | 0.0028 | 0.0001 | 0.0016 | 0.0029 |
Vacuole | 0.0041 | 0.0002 | 0.001 | 0 | 0.0004 | 0.001 | 0.0022 | 0.0003 | 0.0001 | 0 | 0.0005 | 0.0002 | 0.0052 | 0 | 0.0009 | 0.0005 | 0.0044 | 0.0011 |
Vacuole Periphery | 0.0024 | 0.0001 | 0.0001 | 0 | 0.0007 | 0.0007 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0016 | 0 | 0.0007 | 0.0018 | 0.0009 | 0.002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.4661 | 18.2662 | 31.8005 | 29.7605 | 13.2464 | 25.9204 | 24.1517 | 28.7661 | 32.1585 | 17.674 |
Translational Efficiency | 0.7118 | 0.4977 | 0.6811 | 0.6537 | 0.5248 | 0.3011 | 0.557 | 0.6255 | 0.5554 | 0.5022 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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