Standard name
Human Ortholog
Description Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism; mutation is functionally complemented by human DHFR
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
Cytoplasm 0.96 0.89 0.94 0.94 0.77 0.67 0.52 – 0.36 0.37 0.22 0.33 0.98 0.97 0.99 0.98 1.0 0.95 0.9 0.77 0.77 0.79 0.75 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0.07 – 0.06 0.07 0.05 0.05 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0.05
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.22 0.06 – 0.36 0.3 0.5 0.35 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0.05 0.31 0.21 0.44 – 0.34 0.37 0.3 0.25 0 0 0 0 0 0 0 0.09 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 1 2 – 3 2 2 3 0 0 0 0 0 0 0 1 1 0 3 2
Bud 0 0 0 0 1 3 1 – 4 6 6 7 1 0 0 0 0 0 0 0 0 1 2 6
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 4 1 3 2 4 6 – 3 5 3 6 3 2 5 3 0 10 0 0 0 0 0 0
Cytoplasm 326 134 61 140 181 116 158 – 91 89 48 69 183 235 313 90 35 107 313 138 71 74 154 203
Endoplasmic Reticulum 8 5 2 1 0 1 0 – 7 3 2 6 2 2 3 2 1 3 3 0 2 2 5 7
Endosome 3 0 0 1 8 4 20 – 16 17 11 11 0 1 0 0 0 0 11 6 5 6 12 13
Golgi 0 0 0 1 0 0 0 – 1 2 0 2 0 0 0 0 0 0 2 2 1 0 2 3
Mitochondria 1 6 0 3 1 39 17 – 90 71 109 73 0 1 0 1 0 1 1 3 4 1 3 3
Nucleus 1 2 0 0 2 3 6 – 4 4 11 8 0 0 0 0 0 1 1 4 0 0 0 2
Nuclear Periphery 0 1 0 0 0 0 1 – 0 3 0 4 0 0 0 0 0 0 3 1 0 0 1 3
Nucleolus 0 1 0 0 1 0 2 – 0 0 4 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 2 0 0 0 0 0 0 1 0 1 1 3 3
Vac/Vac Membrane 6 1 3 8 72 37 134 – 86 89 64 52 1 3 4 2 0 4 3 15 1 3 9 10
Unique Cell Count 340 151 65 149 234 174 302 251 240 216 208 186 242 317 92 35 113 348 180 94 94 206 274
Labelled Cell Count 349 154 67 157 268 208 347 305 291 260 243 190 245 325 98 36 126 348 180 94 94 206 274


Cytoplasm, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 9.8 9.5 9.1 10.1 6.3 8.8 – 7.4 7.8 6.5 7.6 11.5 9.4 11.1 16.5 16.1 16.9 10.3 11.2 11.5
Std Deviation (1e-4) 1.6 1.9 1.6 1.1 1.4 1.5 1.7 – 1.5 1.1 1.2 1.4 1.4 1.3 1.5 2.9 2.6 3.2 1.8 1.9 2.0
Intensity Change (Log2) – – – -0.05 0.1 -0.59 -0.11 – -0.36 -0.29 -0.53 -0.32 0.29 -0.01 0.23 0.8 0.76 0.84 0.12 0.25 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -3.0 -4.3 -6.2 – -8.3 -8.1 -10.4 -8.5 1.9 1.3 2.5 1.3 1.5 0.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.2 0 – 5.7 5.0 7.3 5.6 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 4.3 3.1 6.0 – 4.7 5.1 4.1 3.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.8726 6.0521 5.5894 4.8825 4.3703 5.4218 7.4823 8.9105 8.1584 7.3089 6.9728 8.1176 9.5541 11.986 10.6899 10.0456 9.1305 10.1628
Actin 0.0241 0.0075 0.015 0.0106 0.0101 0.0121 0.0306 0.0135 0.0346 0.0305 0.0278 0.0113 0.0282 0.0061 0.0244 0.0336 0.0497 0.012
Bud 0.0024 0.0014 0.0045 0.008 0.0023 0.0021 0.0031 0.0015 0.0033 0.0052 0.0072 0.0033 0.002 0.0013 0.0018 0.0194 0.0042 0.0011
Bud Neck 0.0026 0.0007 0.0012 0.0027 0.0014 0.0037 0.0023 0.0014 0.0066 0.0019 0.0027 0.0074 0.0015 0.0009 0.0013 0.0014 0.0015 0.0045
Bud Periphery 0.0013 0.0004 0.0016 0.0033 0.0011 0.0013 0.003 0.0005 0.0024 0.004 0.0043 0.0022 0.0012 0.0003 0.0006 0.0161 0.0016 0.0005
Bud Site 0.0059 0.0021 0.0052 0.0036 0.0013 0.001 0.0124 0.0147 0.0117 0.0366 0.0032 0.0009 0.003 0.0056 0.0045 0.0055 0.0022 0.0006
Cell Periphery 0.0007 0.0005 0.0003 0.0004 0.0001 0.0001 0.0009 0.0004 0.0003 0.0002 0.0004 0.0001 0.0003 0.0001 0.0003 0.0004 0.0001 0.0002
Cytoplasm 0.6053 0.7894 0.7734 0.5427 0.6294 0.7014 0.5464 0.802 0.7608 0.7051 0.4869 0.7648 0.6256 0.8726 0.8577 0.7167 0.6246 0.7752
Cytoplasmic Foci 0.0774 0.0611 0.0575 0.1279 0.1189 0.0676 0.0853 0.0536 0.0602 0.0539 0.1143 0.0424 0.0877 0.0473 0.0313 0.0686 0.0747 0.0634
Eisosomes 0.0002 0 0.0001 0.0001 0.0003 0.0001 0.0004 0 0.0001 0.0002 0.0001 0 0.0002 0 0.0001 0 0.0002 0.0001
Endoplasmic Reticulum 0.0192 0.0132 0.0125 0.0351 0.006 0.0097 0.0456 0.0058 0.0064 0.002 0.0057 0.0169 0.0351 0.0037 0.012 0.0048 0.0058 0.0195
Endosome 0.1402 0.0701 0.0673 0.1455 0.1195 0.1197 0.114 0.0506 0.0549 0.0689 0.133 0.0777 0.1138 0.0229 0.0336 0.0533 0.1313 0.0587
Golgi 0.036 0.0156 0.0255 0.05 0.0265 0.0235 0.0238 0.0178 0.0248 0.0229 0.1047 0.015 0.0276 0.0217 0.0157 0.0276 0.0257 0.0143
Lipid Particles 0.0053 0.0002 0.002 0.0125 0.0048 0.0004 0.0082 0.0004 0.0052 0.0006 0.0048 0.0025 0.0028 0.0002 0.0003 0.0001 0.0182 0.0022
Mitochondria 0.0251 0.0171 0.0122 0.0176 0.0414 0.0221 0.0152 0.004 0.0106 0.0119 0.062 0.0295 0.0103 0.0054 0.0059 0.0379 0.0122 0.018
None 0.008 0.0014 0.003 0.0032 0.0089 0.0028 0.0075 0.0019 0.0015 0.0009 0.0014 0.0031 0.0101 0.0044 0.0031 0.003 0.0033 0.0046
Nuclear Periphery 0.0103 0.0065 0.0028 0.0048 0.0033 0.0045 0.0345 0.0022 0.0024 0.0009 0.0108 0.0039 0.0115 0.0006 0.0012 0.0015 0.0025 0.0023
Nucleolus 0.0026 0 0.0001 0.0003 0.0002 0.0002 0.0006 0.0001 0.0001 0.0001 0.0003 0.0002 0.0002 0 0 0 0.0001 0.0001
Nucleus 0.0066 0.0029 0.0023 0.0039 0.0033 0.0058 0.0139 0.0126 0.002 0.0455 0.0031 0.0059 0.0149 0.0017 0.0017 0.0028 0.002 0.0034
Peroxisomes 0.0105 0.0049 0.0071 0.0123 0.0099 0.0086 0.0191 0.0109 0.0061 0.0035 0.0143 0.0027 0.0132 0.0037 0.0021 0.0046 0.0267 0.0138
Punctate Nuclear 0.0027 0.0004 0.0006 0.0022 0.0024 0.0011 0.0053 0.0006 0.0008 0.0008 0.0008 0.0034 0.0018 0.0003 0.0003 0.0004 0.005 0.0018
Vacuole 0.0096 0.0032 0.004 0.0084 0.005 0.0076 0.0214 0.0044 0.0036 0.0029 0.0053 0.0036 0.0063 0.0008 0.0013 0.0013 0.0045 0.0021
Vacuole Periphery 0.004 0.0015 0.0017 0.0049 0.0038 0.0046 0.0064 0.0011 0.0017 0.0014 0.0069 0.0031 0.0027 0.0003 0.0008 0.001 0.0038 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.0237 23.2636 25.3185 28.3677 39.3643 31.2111 31.3624 31.7512 29.0043 42.7978
Translational Efficiency 1.7168 1.6253 1.2052 0.9913 0.9472 1.2084 1.194 1.0016 1.076 1.078

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism; mutation is functionally complemented by human DHFR
Localization
Cell Percentages cytoplasm (82%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Dfr1

Dfr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dfr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available