Standard name
Human Ortholog
Description AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.27 0.18 0.25 0.35 0.21 0.25 0.24 – 0.19 0.24 0.17 0.22 0.21 0.15 0.11 0.11 0.16 0.12 0.13 0.11 0.12 0.1 0.2 0.17
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.07
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.09 0.11 0.15 0.17 0.08 0.1 0.06 – 0.06 0.07 0.05 0.09 0.3 0.22 0.22 0.21 0.18 0.26 0.11 0.06 0.12 0.06 0.05 0.07
Cytoplasm 0.38 0.36 0.3 0.38 0.15 0.31 0.25 – 0.26 0.22 0.28 0.2 0.47 0.44 0.6 0.36 0.44 0.36 0.23 0.39 0.18 0.37 0.28 0.29
Endoplasmic Reticulum 0.28 0.31 0.22 0.29 0.34 0.29 0.32 – 0.22 0.28 0.19 0.29 0.23 0.26 0.23 0 0.06 0.07 0.26 0.19 0.17 0.14 0.16 0.21
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.21 0.24 0.28 0.15 0.5 0.34 0.35 – 0.29 0.34 0.13 0.28 0.12 0.17 0.13 0.45 0.43 0.42 0.09 0.09 0.12 0.05 0.08 0.06
Mitochondria 0.06 0 0 0 0 0.07 0.13 – 0.26 0.21 0.56 0.37 0 0 0 0 0.06 0 0.09 0.05 0.14 0.08 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 45 18 26 54 59 50 86 – 45 63 39 50 27 27 25 18 27 15 26 16 16 9 46 42
Bud 1 1 1 3 3 3 5 – 8 4 5 2 1 2 0 0 0 1 5 3 3 6 22 17
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 5 1 4 0 7 4
Bud Site 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 15 11 16 26 22 19 23 – 14 18 11 21 38 40 48 33 31 32 21 8 15 5 10 17
Cytoplasm 63 36 31 60 43 61 89 – 63 58 65 45 60 81 134 57 74 44 46 58 23 33 65 73
Endoplasmic Reticulum 47 31 23 45 95 59 117 – 53 75 43 67 29 47 52 5 10 9 53 28 21 12 36 53
Endosome 0 0 0 0 0 0 3 – 0 2 0 2 1 0 0 0 0 0 1 3 2 1 3 6
Golgi 35 24 29 24 139 68 126 – 69 90 29 65 15 31 29 72 73 52 18 13 15 4 18 15
Mitochondria 10 0 0 0 1 14 48 – 61 57 129 84 1 1 0 4 11 1 17 7 18 6 7 10
Nucleus 0 0 0 0 0 2 1 – 0 1 0 0 0 0 1 0 0 0 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 1 0 0 0 1 – 0 0 1 0 0 2 0 0 0 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 3 4 0 10 5 12 – 12 17 5 10 0 6 1 7 3 3 5 5 5 5 9 7
Unique Cell Count 165 101 104 156 279 200 361 238 267 231 230 127 183 222 160 170 123 206 150 130 89 232 254
Labelled Cell Count 216 124 131 212 372 281 511 325 386 328 346 173 237 290 196 230 159 206 150 130 89 232 254


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.1 27.0 21.0 18.9 20.3 16.5 16.0 – 13.1 13.2 11.4 11.9 21.5 20.9 21.6 35.5 35.5 33.2 20.8 21.2 22.6
Std Deviation (1e-4) 3.7 4.6 4.3 3.7 3.3 3.0 2.7 – 2.4 2.6 2.3 2.3 4.3 5.9 4.1 11.0 9.8 9.1 4.6 4.9 5.0
Intensity Change (Log2) – – – -0.15 -0.05 -0.35 -0.4 – -0.69 -0.67 -0.89 -0.82 0.03 -0.01 0.04 0.75 0.75 0.66 -0.01 0.01 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.6 -0.8 0 -0.2 – -1.3 -0.3 -1.7 -0.7 -0.7 -2.2 -3.2 -2.9 -1.9 -2.5
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.3 -2.2 -1.5 -2.9 – -2.9 -2.6 -3.3 -1.7 2.6 1.3 1.3 1.1 0.6 2.0
Cytoplasm 1.4 -3.2 0.1 -1.1 – -0.6 -1.6 -0.3 -2.1 2.7 2.4 5.1 1.0 2.3 1.0
Endoplasmic Reticulum 1.2 2.2 1.4 2.0 – 0 1.2 -0.7 1.3 0.1 0.7 0.3 -4.9 -4.0 -3.2
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi -2.5 3.8 1.1 1.3 – 0.2 1.1 -3.4 0.1 -3.1 -2.2 -3.3 2.8 2.5 2.3
Mitochondria 0 0 0 3.9 – 5.7 5.1 9.7 7.1 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0.9 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.7378 15.9461 14.6157 12.5225 12.1916 14.9943 17.3912 18.8739 18.4033 17.5849 16.4952 17.5167 17.8801 18.4791 18.987 17.2406 16.4952 17.5949
Actin 0.2589 0.2172 0.1893 0.1495 0.1452 0.1403 0.1802 0.1349 0.1233 0.1393 0.1226 0.1265 0.1806 0.142 0.1382 0.1662 0.1295 0.1734
Bud 0.0088 0.0205 0.0154 0.0071 0.0048 0.0063 0.0111 0.0143 0.0098 0.0107 0.0051 0.0067 0.0127 0.0141 0.014 0.0079 0.0052 0.007
Bud Neck 0.0082 0.0048 0.0244 0.033 0.0228 0.0573 0.0059 0.0051 0.0281 0.0162 0.0277 0.053 0.0055 0.0051 0.0258 0.032 0.0139 0.0538
Bud Periphery 0.0125 0.0141 0.0269 0.025 0.0111 0.0197 0.0224 0.0142 0.0251 0.0347 0.0193 0.0221 0.0155 0.0135 0.0167 0.0357 0.0153 0.0184
Bud Site 0.0264 0.1483 0.033 0.0041 0.0032 0.0037 0.0356 0.1456 0.0384 0.0078 0.0038 0.0037 0.0279 0.146 0.0394 0.0047 0.0021 0.0027
Cell Periphery 0.0169 0.0136 0.0261 0.042 0.0143 0.0435 0.0433 0.0169 0.0382 0.0573 0.0287 0.0395 0.0163 0.0208 0.0176 0.0223 0.01 0.0167
Cytoplasm 0.1528 0.1971 0.1755 0.1069 0.1986 0.1185 0.2099 0.2537 0.1885 0.1721 0.3015 0.1691 0.1999 0.2929 0.2323 0.1542 0.1888 0.1906
Cytoplasmic Foci 0.0453 0.067 0.0273 0.0167 0.0545 0.025 0.0321 0.0662 0.0367 0.0261 0.0325 0.0323 0.0373 0.0499 0.0352 0.0332 0.0646 0.0355
Eisosomes 0.008 0.0024 0.0046 0.0066 0.0012 0.0087 0.0114 0.0042 0.0103 0.0265 0.0067 0.0101 0.0113 0.0047 0.0056 0.0026 0.0031 0.008
Endoplasmic Reticulum 0.0403 0.0115 0.0549 0.1335 0.0307 0.0654 0.0703 0.0181 0.0697 0.0784 0.1078 0.075 0.0681 0.0389 0.0503 0.1279 0.0769 0.0542
Endosome 0.0881 0.0723 0.0936 0.0931 0.1155 0.09 0.0777 0.0868 0.0959 0.0639 0.065 0.0781 0.1037 0.0685 0.1026 0.0877 0.0872 0.0878
Golgi 0.1553 0.1409 0.1621 0.1309 0.2461 0.2631 0.1752 0.1562 0.192 0.1562 0.1822 0.2527 0.2111 0.1334 0.2018 0.2257 0.2504 0.2527
Lipid Particles 0.0215 0.0031 0.0054 0.0185 0.0148 0.0012 0.0046 0.0026 0.0033 0.0035 0.0025 0.0019 0.0039 0.0022 0.0032 0.0027 0.003 0.0013
Mitochondria 0.0995 0.0672 0.117 0.1814 0.0975 0.1438 0.084 0.0654 0.1062 0.1772 0.081 0.1137 0.0726 0.0524 0.088 0.0751 0.14 0.081
None 0.0038 0.0006 0.0011 0.0014 0.0025 0.001 0.004 0.0011 0.0013 0.003 0.0027 0.0015 0.0018 0.0008 0.0009 0.0017 0.001 0.0011
Nuclear Periphery 0.013 0.0007 0.0043 0.0054 0.0014 0.0011 0.0034 0.0008 0.0022 0.0027 0.0024 0.0012 0.0082 0.0014 0.0024 0.0037 0.0011 0.0013
Nucleolus 0.0022 0.0004 0.0005 0.0002 0.0002 0.0001 0.0004 0.0002 0.0003 0.0003 0.0001 0.0002 0.0007 0.0002 0.0004 0.0001 0.0001 0.0002
Nucleus 0.0057 0.001 0.0016 0.0015 0.0008 0.0006 0.0014 0.0011 0.0013 0.0016 0.0008 0.0007 0.0035 0.0009 0.0015 0.001 0.0004 0.0009
Peroxisomes 0.0098 0.0034 0.0095 0.0033 0.0207 0.0007 0.0011 0.0024 0.0014 0.0017 0.0008 0.0009 0.0013 0.0019 0.0012 0.001 0.0017 0.001
Punctate Nuclear 0.006 0.0003 0.0003 0.0002 0.0017 0.0001 0.0004 0.0003 0.0003 0.0002 0.0002 0.0001 0.0007 0.0003 0.0002 0.0002 0.0002 0.0002
Vacuole 0.0107 0.0107 0.0179 0.0265 0.0082 0.0056 0.0199 0.0078 0.0197 0.0137 0.0045 0.0069 0.0121 0.0078 0.0159 0.0091 0.0032 0.0077
Vacuole Periphery 0.0062 0.0029 0.0094 0.0132 0.0042 0.0042 0.0056 0.0021 0.008 0.0068 0.002 0.004 0.0053 0.0023 0.0068 0.0051 0.0024 0.0046

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 78.0628 80.4992 70.5946 54.7664 57.9978 96.4287 78.75 76.3376 64.892 50.704
Translational Efficiency 3.0836 2.9952 2.3258 2.701 2.4214 2.1257 2.0583 2.1078 1.8305 2.1775

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift
Localization
Cell Percentages cytoplasm (21%), ER (7%), mixed (48%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Abp140

Abp140


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Abp140-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available