Standard name | |
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Human Ortholog | |
Description | Pseudouridine synthase; catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); also pseudouridylates some mRNAs; relocates from nucleus to cytoplasm during heat shock and differentially modifies some mRNAs during heat shock; conserved in archaea, vertebrates, and some bacteria |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 2 | 2 | 0 | 1 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 3 | 8 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 6 | 2 | 2 | 15 | 23 | 12 | 17 | 15 | 12 | 8 | 7 | 11 | 25 | 17 | – | – | – | 0 | 0 | 0 | 1 | 0 | 3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | – | – | – | 0 | 1 | 0 | 0 | 0 | 1 |
Mitochondria | 2 | 2 | 1 | 1 | 0 | 3 | 6 | 8 | 7 | 6 | 33 | 17 | 0 | 0 | 0 | – | – | – | 1 | 1 | 0 | 0 | 2 | 1 |
Nucleus | 191 | 243 | 39 | 39 | 158 | 155 | 226 | 242 | 106 | 220 | 169 | 231 | 140 | 163 | 138 | – | – | – | 189 | 242 | 64 | 96 | 145 | 143 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 2 | 2 | 0 | 1 | 0 | 1 | 0 | 2 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 2 | 0 | 2 | 2 | 1 | 5 | 18 | 12 | 18 | 34 | 53 | 49 | 2 | 1 | 13 | – | – | – | 0 | 0 | 0 | 0 | 5 | 12 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 3 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 2 | 0 | 0 | 3 | 1 | 0 | 1 | 1 | 3 | 0 | 6 | 4 | 0 | 1 | – | – | – | 8 | 7 | 0 | 6 | 29 | 42 |
Unique Cell Count | 200 | 253 | 41 | 41 | 175 | 186 | 254 | 276 | 136 | 256 | 209 | 271 | 154 | 194 | 169 | 205 | 260 | 69 | 109 | 191 | 213 | |||
Labelled Cell Count | 204 | 253 | 44 | 44 | 178 | 188 | 269 | 284 | 153 | 280 | 268 | 321 | 160 | 196 | 177 | 205 | 260 | 69 | 109 | 191 | 213 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.6 | 11.2 | 8.5 | 8.5 | 10.4 | 8.5 | 8.0 | 8.3 | 7.6 | 7.9 | 6.6 | 7.1 | 13.3 | 11.6 | 10.9 | – | – | – | 9.7 | 11.1 | 11.0 |
Std Deviation (1e-4) | 1.8 | 2.4 | 1.7 | 1.7 | 2.2 | 2.3 | 1.9 | 2.0 | 1.9 | 2.0 | 1.7 | 1.7 | 13.6 | 3.4 | 2.8 | – | – | – | 2.2 | 2.5 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.28 | -0.0 | -0.08 | -0.04 | -0.17 | -0.1 | -0.36 | -0.26 | 0.64 | 0.45 | 0.36 | – | – | – | 0.2 | 0.39 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 2.1 | 0 | 0 | 0 | – | – | – |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleus | – | -1.0 | -1.9 | -1.2 | -1.4 | -2.5 | -1.6 | -2.2 | -1.7 | -0.9 | -1.9 | -2.1 | – | – | – |
Vacuole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.4855 | 10.1061 | 8.8058 | 8.4679 | 8.5563 | 8.0816 | 9.3725 | 9.2793 | 8.5831 | 8.8132 | 7.9535 | 8.2395 | 11.4294 | 10.617 | 10.1324 | 9.8483 | 10.1115 | 9.8775 |
Actin | 0.0069 | 0 | 0.0015 | 0.0011 | 0 | 0.0013 | 0.0044 | 0 | 0.0038 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0082 |
Bud | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 |
Bud Neck | 0.0003 | 0 | 0 | 0 | 0.0002 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0003 | 0 | 0.0002 |
Bud Periphery | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0006 | 0 | 0.0001 |
Bud Site | 0.0009 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0004 | 0 | 0 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0 |
Cytoplasm | 0.0098 | 0 | 0.0012 | 0 | 0.0001 | 0.0008 | 0.001 | 0 | 0.0004 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 |
Cytoplasmic Foci | 0.0084 | 0 | 0.0042 | 0.0039 | 0 | 0.0016 | 0.004 | 0 | 0.0008 | 0 | 0 | 0 | 0.0008 | 0 | 0 | 0 | 0 | 0.0004 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0022 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.001 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 |
Endosome | 0.0131 | 0 | 0.0024 | 0.0079 | 0.0002 | 0.0144 | 0.0027 | 0 | 0.0006 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0009 |
Golgi | 0.0018 | 0 | 0.0004 | 0.0098 | 0 | 0.0041 | 0.0009 | 0 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0009 |
Lipid Particles | 0.0038 | 0 | 0.0018 | 0.0229 | 0.0001 | 0.004 | 0.0023 | 0 | 0.0019 | 0 | 0 | 0 | 0.0009 | 0 | 0 | 0 | 0 | 0.0012 |
Mitochondria | 0.0061 | 0.0003 | 0.0011 | 0.0048 | 0.0008 | 0.0014 | 0.0015 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0.0001 | 0.0007 | 0.0002 | 0.0009 | 0.0007 | 0.0001 | 0.0004 |
None | 0.0024 | 0 | 0.0007 | 0 | 0 | 0.0004 | 0.004 | 0 | 0.0004 | 0 | 0 | 0 | 0.0006 | 0 | 0 | 0 | 0 | 0.0001 |
Nuclear Periphery | 0.0218 | 0.0021 | 0.0027 | 0.0006 | 0.0031 | 0.0141 | 0.0018 | 0.0005 | 0.0018 | 0.001 | 0.0013 | 0.0002 | 0.0004 | 0.0005 | 0.001 | 0.0003 | 0.0002 | 0.0007 |
Nucleolus | 0.0339 | 0.0069 | 0.0072 | 0.0043 | 0.1181 | 0.0731 | 0.0325 | 0.022 | 0.0065 | 0.0043 | 0.1648 | 0.0393 | 0.0103 | 0.0098 | 0.006 | 0.0164 | 0.0725 | 0.0267 |
Nucleus | 0.8663 | 0.9904 | 0.9657 | 0.9398 | 0.8698 | 0.8761 | 0.9307 | 0.9769 | 0.9791 | 0.9945 | 0.8306 | 0.9599 | 0.984 | 0.9891 | 0.9913 | 0.9787 | 0.9268 | 0.9587 |
Peroxisomes | 0.0024 | 0 | 0.0037 | 0.0035 | 0 | 0.0004 | 0.0097 | 0 | 0.0012 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0005 |
Punctate Nuclear | 0.0144 | 0.0002 | 0.0044 | 0.0004 | 0.0003 | 0.0029 | 0.0024 | 0.0001 | 0.0009 | 0 | 0.0022 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0006 |
Vacuole | 0.0025 | 0 | 0.001 | 0.0002 | 0.0034 | 0.0012 | 0.0003 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0011 | 0 | 0.0001 |
Vacuole Periphery | 0.0021 | 0.0001 | 0.001 | 0.0003 | 0.0032 | 0.001 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 76.6027 | 77.22 | 59.8232 | 58.156 | 53.8857 | 43.9814 | 54.2611 | 51.5368 | 43.6829 | 61.2042 |
Translational Efficiency | 1.3669 | 1.2721 | 1.3758 | 1.2 | 1.4322 | 1.679 | 1.2125 | 0.8635 | 1.0034 | 1.0776 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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