Standard name
Human Ortholog
Description Gamma subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 0.06 0.06 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0.05 0.05 0.06 0.11 0.12 0.11 0.1 0.14 0 0 0 0.06 0.05 0.09 0 0 0 0 0 0
Cytoplasm 0.97 0.94 0.84 0.89 0.74 0.63 0.73 0.7 0.62 0.61 0.58 0.57 0.77 0.82 0.85 0.93 0.92 0.92 0.94 0.93 0.79 0.69 0.74 0.77
Endoplasmic Reticulum 0 0.06 0.12 0.08 0.07 0.17 0.18 0.09 0.12 0.14 0.12 0.11 0.19 0.12 0.1 0.08 0 0.07 0 0 0.13 0.18 0.16 0.15
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.07 0.23 0.05 0 0.18 0.15 0.31 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.11 0 0.06 0.11 0.09 0.07 0 0.1 0.06 0.07 0 0 0 0 0 0 0 0.06 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 5 13 7 11 25 20 47 77 71 80 96 2 0 1 0 1 0 0 4 4 1 0 1
Bud 0 6 2 0 0 0 0 2 7 3 18 3 0 0 0 0 0 0 1 3 4 1 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 24 40 46 50 64 65 108 160 123 134 155 2 3 13 6 4 7 0 0 11 0 0 0
Cytoplasm 164 942 1098 786 773 789 783 718 814 685 751 626 168 305 274 101 70 70 163 956 925 204 369 303
Endoplasmic Reticulum 4 59 157 67 70 214 191 95 161 160 156 120 41 43 33 9 2 5 1 27 156 52 78 57
Endosome 0 0 1 0 3 0 0 0 3 2 3 0 1 1 0 0 0 0 0 1 4 1 1 0
Golgi 0 2 48 9 33 4 23 52 34 30 12 32 5 7 5 1 1 1 1 2 22 4 7 5
Mitochondria 1 2 8 9 77 289 51 26 242 169 402 247 0 0 1 1 0 1 0 2 12 7 4 5
Nucleus 1 0 1 0 3 3 7 6 1 2 3 2 0 1 2 0 1 0 1 2 0 0 0 0
Nuclear Periphery 0 0 0 1 2 16 1 1 1 12 3 14 0 0 0 0 0 0 0 1 4 0 2 0
Nucleolus 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0
Vac/Vac Membrane 0 4 30 24 114 38 59 111 117 78 56 116 13 26 13 4 2 2 3 16 13 18 24 14
Unique Cell Count 169 1003 1304 887 1043 1258 1068 1028 1317 1119 1303 1107 218 370 324 109 76 76 173 1028 1172 297 500 396
Labelled Cell Count 173 1044 1398 949 1137 1443 1201 1166 1618 1336 1619 1411 232 386 342 122 81 86 173 1028 1172 297 500 396


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.5 20.8 18.7 16.3 13.8 11.4 12.9 15.5 12.0 12.3 11.0 11.5 19.0 19.8 19.5 20.8 19.9 20.2 17.4 17.9 19.1
Std Deviation (1e-4) 3.0 3.4 3.3 2.6 2.6 2.6 2.9 2.5 2.6 2.4 2.3 2.3 3.6 3.2 3.7 6.3 6.2 6.3 3.3 3.5 3.8
Intensity Change (Log2) – – – -0.2 -0.45 -0.72 -0.54 -0.28 -0.64 -0.61 -0.76 -0.7 0.02 0.08 0.06 0.15 0.09 0.11 -0.11 -0.06 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.5 0.1 2.1 1.8 5.4 6.8 7.2 7.1 9.0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 3.6 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.5 2.2 2.6 3.6 7.3 8.8 7.8 7.4 9.8 -1.8 -2.4 0.9 0 0 0
Cytoplasm 2.9 -6.0 -12.3 -6.5 -8.3 -12.9 -12.8 -14.9 -15.0 -2.6 -0.8 0.2 2.4 1.9 1.9
Endoplasmic Reticulum -3.4 -4.3 3.6 4.0 -2.2 0.1 1.6 -0.1 -0.9 2.7 -0.2 -0.9 -1.2 -2.5 -1.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -3.8 -0.7 -6.0 -2.2 1.6 -1.6 -1.4 -4.7 -1.1 -1.0 -1.7 -1.9 0 0 0
Mitochondria 1.1 8.7 17.7 6.5 3.8 15.5 13.7 21.2 17.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 4.1 0 0 0 3.7 0 4.1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.6 8.7 1.1 4.1 8.5 7.3 5.6 2.9 8.4 3.0 4.5 1.7 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.3575 17.1217 14.5364 13.5065 14.6242 14.6142 14.9668 19.5213 17.9246 16.1415 16.5365 17.6181 10.9471 17.2819 15.7923 13.1162 14.031 13.9975
Actin 0.0042 0.0023 0.0023 0.0019 0.0045 0.0027 0.0048 0.0014 0.0022 0.0031 0.0021 0.0014 0.0201 0.0018 0.0139 0.0962 0.0183 0.0013
Bud 0.0013 0.001 0.0019 0.0017 0.001 0.0022 0.0014 0.0011 0.0015 0.0022 0.0007 0.0009 0.0019 0.0008 0.0013 0.0026 0.0012 0.0004
Bud Neck 0.0006 0.0005 0.0006 0.0006 0.0006 0.0018 0.0007 0.0006 0.0008 0.001 0.0009 0.0013 0.0009 0.0003 0.0006 0.0009 0.0003 0.0008
Bud Periphery 0.0011 0.0004 0.0008 0.0005 0.0003 0.0007 0.001 0.0007 0.0008 0.0012 0.0002 0.0005 0.0016 0.0001 0.0007 0.0008 0.0014 0.0001
Bud Site 0.0011 0.0015 0.0013 0.0007 0.0003 0.0003 0.0013 0.001 0.001 0.0013 0.0003 0.0002 0.0056 0.0018 0.001 0.002 0.0004 0.0001
Cell Periphery 0.0012 0.0005 0.0004 0.0001 0.0001 0.0001 0.0009 0.0005 0.0004 0.0003 0.0002 0.0004 0.0005 0.0002 0.0004 0.0002 0.0001 0
Cytoplasm 0.6268 0.8193 0.7409 0.6658 0.5703 0.6443 0.6597 0.7984 0.6683 0.5814 0.5527 0.6616 0.5813 0.8573 0.7143 0.4627 0.5716 0.6418
Cytoplasmic Foci 0.0293 0.0254 0.0317 0.0361 0.0467 0.0379 0.0375 0.0297 0.0396 0.0491 0.0537 0.0476 0.0407 0.0235 0.0346 0.0452 0.0266 0.0298
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 0.0003 0 0.0001 0.0003 0.0003 0
Endoplasmic Reticulum 0.0623 0.0161 0.0154 0.0197 0.0056 0.0348 0.0264 0.0231 0.0151 0.0129 0.0198 0.0338 0.0644 0.0103 0.0163 0.0036 0.0047 0.0509
Endosome 0.1557 0.0914 0.142 0.1883 0.2791 0.1974 0.1646 0.092 0.1684 0.2492 0.2623 0.1667 0.154 0.0635 0.1363 0.2764 0.2094 0.1867
Golgi 0.0321 0.0176 0.0171 0.0138 0.065 0.0419 0.0549 0.0263 0.056 0.0388 0.078 0.0555 0.0428 0.0195 0.0269 0.0359 0.0262 0.0572
Lipid Particles 0.0013 0.0006 0.0002 0.0178 0.0001 0.0001 0.0006 0.0004 0.0004 0.0003 0.0005 0.0002 0.0021 0.0001 0.002 0.0005 0.0005 0.0003
Mitochondria 0.016 0.0041 0.0059 0.0022 0.0077 0.011 0.0101 0.0071 0.0063 0.0074 0.0057 0.0128 0.0276 0.0017 0.0116 0.0038 0.0951 0.0043
None 0.004 0.0004 0.0006 0.0005 0.0003 0.0005 0.0008 0.0004 0.0005 0.0005 0.0004 0.0005 0.0239 0.0004 0.0008 0.0004 0.0003 0.0005
Nuclear Periphery 0.0095 0.0015 0.0023 0.0044 0.0009 0.0024 0.0022 0.0009 0.0014 0.0017 0.0013 0.001 0.007 0.0033 0.0035 0.0043 0.0025 0.0027
Nucleolus 0.0004 0 0.0001 0.0001 0 0 0 0 0 0 0 0 0.0002 0 0 0.0001 0 0
Nucleus 0.0027 0.001 0.0013 0.0019 0.0005 0.0013 0.0011 0.0007 0.0009 0.0011 0.0008 0.0009 0.0019 0.0009 0.0013 0.002 0.0007 0.001
Peroxisomes 0.0003 0.0002 0.0002 0.0045 0.0003 0.0003 0.0002 0.0002 0.0002 0.0003 0.0004 0.0002 0.0014 0.0001 0.0009 0.0007 0.0002 0.0002
Punctate Nuclear 0.0025 0.0001 0.0002 0.0022 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0009 0.0001 0.0002 0.0002 0.0001 0.0001
Vacuole 0.038 0.0124 0.0262 0.0245 0.0097 0.0126 0.0242 0.0122 0.0273 0.0356 0.0124 0.0094 0.0145 0.01 0.0234 0.0434 0.0242 0.0127
Vacuole Periphery 0.0097 0.0038 0.0089 0.0126 0.0071 0.0075 0.0072 0.003 0.0089 0.0126 0.0076 0.0048 0.0064 0.0043 0.0098 0.0179 0.0157 0.0092

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 84.4864 60.7612 80.0618 84.407 84.4201 62.8535 68.0385 80.9922 71.5072 81.9654
Translational Efficiency 0.8312 0.9127 0.7785 0.676 0.6174 0.8924 0.7621 0.5615 0.6704 0.6768

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Gamma subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
Localization
Cell Percentages cytoplasm (91%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Gcd1

Gcd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gcd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available