Standard name | |
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Human Ortholog | |
Description | Mitochondrial integral inner membrane protein; involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 26 | 40 | 13 | – | 0 | – | 0 | 1 | 2 | 1 | 16 | 33 |
Bud | 4 | 10 | 6 | 4 | 4 | 5 | 11 | 24 | 19 | 16 | 27 | 26 | 9 | 6 | 5 | – | 1 | – | 1 | 1 | 0 | 0 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | – | 0 | – | 0 | 0 | 0 | 1 | 5 | 8 |
Bud Site | 0 | 0 | 1 | 0 | 1 | 5 | 7 | 12 | 9 | 8 | 8 | 10 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 9 | 10 | 2 | 2 | 4 | 5 | 3 | 3 | 5 | 8 | 8 | 10 | 14 | – | 7 | – | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 3 | 4 | 1 | 5 | 9 | 11 | 4 | 17 | 13 | 10 | 4 | 4 | 6 | 13 | 25 | – | 2 | – | 0 | 0 | 0 | 0 | 1 | 1 |
Endoplasmic Reticulum | 0 | 5 | 2 | 0 | 1 | 1 | 2 | 1 | 3 | 1 | 2 | 3 | 24 | 27 | 12 | – | 1 | – | 0 | 0 | 1 | 2 | 6 | 1 |
Endosome | 6 | 0 | 41 | 38 | 37 | 35 | 26 | 36 | 5 | 7 | 1 | 3 | 13 | 20 | 17 | – | 0 | – | 13 | 6 | 24 | 22 | 55 | 47 |
Golgi | 4 | 2 | 2 | 2 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 15 | 49 | 36 | – | 1 | – | 2 | 5 | 16 | 7 | 42 | 49 |
Mitochondria | 138 | 151 | 302 | 262 | 360 | 412 | 537 | 805 | 510 | 639 | 668 | 752 | 163 | 127 | 80 | – | 2 | – | 132 | 117 | 269 | 176 | 546 | 532 |
Nucleus | 1 | 1 | 1 | 0 | 3 | 16 | 13 | 21 | 10 | 9 | 7 | 5 | 2 | 1 | 4 | – | 2 | – | 0 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 4 | 1 | 9 | 3 | 8 | 19 | 60 | 32 | 36 | 32 | 38 | 26 | 0 | 3 | 0 | – | 0 | – | 4 | 0 | 2 | 2 | 3 | 9 |
Nucleolus | 4 | 1 | 3 | 5 | 10 | 38 | 51 | 75 | 42 | 44 | 35 | 23 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 5 | 2 |
Peroxisomes | 2 | 0 | 36 | 31 | 17 | 16 | 13 | 46 | 5 | 10 | 7 | 7 | 3 | 7 | 4 | – | 5 | – | 4 | 3 | 38 | 20 | 63 | 56 |
SpindlePole | 2 | 1 | 15 | 12 | 13 | 18 | 20 | 35 | 20 | 21 | 17 | 25 | 19 | 15 | 10 | – | 1 | – | 2 | 0 | 1 | 1 | 4 | 4 |
Vac/Vac Membrane | 10 | 17 | 22 | 14 | 47 | 48 | 34 | 46 | 21 | 15 | 26 | 38 | 36 | 35 | 54 | – | 2 | – | 3 | 10 | 0 | 8 | 29 | 23 |
Unique Cell Count | 152 | 153 | 347 | 292 | 417 | 492 | 617 | 927 | 565 | 681 | 692 | 775 | 246 | 272 | 216 | 22 | 169 | 152 | 360 | 254 | 792 | 778 | ||
Labelled Cell Count | 181 | 196 | 450 | 386 | 515 | 627 | 782 | 1156 | 696 | 815 | 846 | 931 | 324 | 354 | 276 | 24 | 169 | 152 | 360 | 254 | 792 | 778 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 4.4 | 5.3 | 5.4 | 5.4 | 4.8 | 4.5 | 4.7 | 4.4 | 4.1 | 4.1 | 4.3 | 6.2 | 6.5 | 6.5 | – | 5.7 | – | 6.5 | 6.7 | 6.9 |
Std Deviation (1e-4) | 0.7 | 0.5 | 1.0 | 1.0 | 1.7 | 1.2 | 1.1 | 1.2 | 1.2 | 1.1 | 0.9 | 0.9 | 0.7 | 0.8 | 0.8 | – | 1.7 | – | 1.1 | 1.5 | 1.1 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.04 | -0.14 | -0.24 | -0.17 | -0.25 | -0.37 | -0.36 | -0.29 | 0.23 | 0.3 | 0.3 | – | 0.11 | – | 0.32 | 0.35 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3506 | 2.168 | 1.7457 | 1.7592 | 1.9665 | 2.2198 | 1.192 | 2.091 | 1.7492 | 1.381 | 1.895 | 1.8297 | 1.088 | 1.6189 | 1.7691 | 2.0847 | 1.6761 | 1.557 |
Actin | 0.0371 | 0.0064 | 0.011 | 0.0033 | 0.0114 | 0.0112 | 0.0287 | 0.0144 | 0.0126 | 0.0003 | 0.0027 | 0.0097 | 0.0037 | 0.0118 | 0.0129 | 0.0003 | 0.0024 | 0.0044 |
Bud | 0.0008 | 0.002 | 0.0008 | 0.0027 | 0.0006 | 0.0004 | 0.0004 | 0.0007 | 0.0007 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0.0007 | 0.0005 | 0.0002 | 0.0009 | 0.0004 |
Bud Neck | 0.0008 | 0.001 | 0.0063 | 0.0003 | 0.0015 | 0.001 | 0.0004 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0011 | 0.0001 | 0.0001 | 0.0007 |
Bud Periphery | 0.0013 | 0.005 | 0.0018 | 0.009 | 0.0011 | 0.001 | 0.0011 | 0.0012 | 0.0019 | 0.0005 | 0.0002 | 0.0012 | 0.0013 | 0.0007 | 0.0009 | 0.0005 | 0.0044 | 0.0008 |
Bud Site | 0.0024 | 0.0184 | 0.0102 | 0.0023 | 0.0021 | 0.0006 | 0.0013 | 0.0021 | 0.0019 | 0.0002 | 0.0002 | 0.0045 | 0.001 | 0.0023 | 0.0014 | 0.0008 | 0.0007 | 0.0005 |
Cell Periphery | 0.0004 | 0.001 | 0.0005 | 0.0012 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0006 | 0.0002 | 0.0001 | 0.0002 | 0.0029 | 0.0002 | 0.0005 | 0.0003 | 0.0008 | 0.0004 |
Cytoplasm | 0.0064 | 0.0058 | 0.0018 | 0.0086 | 0.0064 | 0.0028 | 0.0008 | 0.0017 | 0.0036 | 0.0006 | 0.0004 | 0.0011 | 0.0006 | 0.0464 | 0.0007 | 0.001 | 0.0004 | 0.0025 |
Cytoplasmic Foci | 0.0228 | 0.0288 | 0.0279 | 0.0357 | 0.03 | 0.0318 | 0.0309 | 0.0258 | 0.0159 | 0.0093 | 0.0151 | 0.013 | 0.0173 | 0.0158 | 0.0185 | 0.0013 | 0.0061 | 0.0149 |
Eisosomes | 0.0005 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0006 | 0.0003 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0003 | 0.0015 | 0 | 0.0004 | 0.0004 |
Endoplasmic Reticulum | 0.0037 | 0.0004 | 0.0005 | 0.0008 | 0.0035 | 0.002 | 0.001 | 0.0006 | 0.0017 | 0.0002 | 0.0001 | 0.0006 | 0.0015 | 0.0013 | 0.0009 | 0.0019 | 0.0002 | 0.0003 |
Endosome | 0.0701 | 0.0363 | 0.0404 | 0.0351 | 0.0944 | 0.0837 | 0.0416 | 0.0371 | 0.0382 | 0.0821 | 0.0419 | 0.0362 | 0.0346 | 0.0417 | 0.0476 | 0.0527 | 0.0245 | 0.0296 |
Golgi | 0.0594 | 0.0507 | 0.0413 | 0.0217 | 0.0468 | 0.0779 | 0.055 | 0.0565 | 0.0368 | 0.0191 | 0.068 | 0.0458 | 0.0362 | 0.104 | 0.0594 | 0.0043 | 0.0648 | 0.0449 |
Lipid Particles | 0.0139 | 0.009 | 0.0108 | 0.0274 | 0.0195 | 0.0147 | 0.0474 | 0.0093 | 0.02 | 0.004 | 0.0389 | 0.0178 | 0.1211 | 0.0124 | 0.0339 | 0.0033 | 0.0253 | 0.0073 |
Mitochondria | 0.7192 | 0.792 | 0.8029 | 0.7751 | 0.6967 | 0.7321 | 0.6923 | 0.8143 | 0.7779 | 0.8424 | 0.7905 | 0.8367 | 0.6697 | 0.7369 | 0.7535 | 0.8793 | 0.8572 | 0.8695 |
None | 0.0076 | 0.002 | 0.0014 | 0.0098 | 0.0038 | 0.0012 | 0.0037 | 0.0005 | 0.0097 | 0.0001 | 0.0001 | 0.0013 | 0.0015 | 0.0004 | 0.0013 | 0.0005 | 0.0002 | 0.0032 |
Nuclear Periphery | 0.0156 | 0.0001 | 0.0009 | 0.0004 | 0.0273 | 0.0005 | 0.0014 | 0.0001 | 0.0076 | 0.0001 | 0 | 0.0002 | 0.0015 | 0.0003 | 0.0006 | 0.0065 | 0.0001 | 0.0001 |
Nucleolus | 0.0004 | 0.0005 | 0.001 | 0.0007 | 0.0012 | 0.0002 | 0.0032 | 0.0001 | 0.0008 | 0.0002 | 0 | 0.0001 | 0.0024 | 0.0002 | 0.0014 | 0.0008 | 0.0001 | 0.0001 |
Nucleus | 0.0038 | 0.0005 | 0.001 | 0.0006 | 0.0031 | 0.0003 | 0.0007 | 0.0001 | 0.0013 | 0.0002 | 0 | 0.0002 | 0.0011 | 0.0002 | 0.0006 | 0.0028 | 0.0001 | 0.0001 |
Peroxisomes | 0.019 | 0.0339 | 0.0282 | 0.0527 | 0.0294 | 0.0284 | 0.0811 | 0.0306 | 0.0498 | 0.017 | 0.0392 | 0.0266 | 0.0468 | 0.0192 | 0.0545 | 0.0006 | 0.0067 | 0.0167 |
Punctate Nuclear | 0.0065 | 0.0003 | 0.0009 | 0.0013 | 0.0149 | 0.0004 | 0.0024 | 0.0001 | 0.0007 | 0 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 |
Vacuole | 0.0034 | 0.0036 | 0.0067 | 0.0075 | 0.0026 | 0.0047 | 0.0026 | 0.0022 | 0.0111 | 0.0145 | 0.0006 | 0.0016 | 0.0374 | 0.0024 | 0.0047 | 0.0285 | 0.0016 | 0.0016 |
Vacuole Periphery | 0.0048 | 0.002 | 0.0033 | 0.0037 | 0.0032 | 0.0046 | 0.003 | 0.0016 | 0.0062 | 0.0086 | 0.0015 | 0.0021 | 0.0177 | 0.0023 | 0.0033 | 0.014 | 0.0027 | 0.0016 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.0118 | 5.4282 | 5.7542 | 8.8065 | 9.5599 | 8.6698 | 6.8605 | 7.4086 | 6.7677 | 11.7602 |
Translational Efficiency | 0.5561 | 0.8177 | 0.9804 | 0.575 | 0.6085 | 0.8997 | 0.8466 | 0.8378 | 0.9684 | 0.7168 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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