Standard name
Human Ortholog
Description Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.29 0.34 0.39 0.35 0.35 0.45 0.5 0.41 – 0.46 0.32 0.39 0.19 0.14 0.08 0 0.07 0.08 0.06 0.05 0 0 0 0
Bud 0.11 0.08 0 0.07 0.07 0.05 0 0.05 – 0.05 0.12 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.6 0.78 0.86 0.77 0.79 0.83 0.76 0.67 – 0.72 0.7 0.75 0.93 0.91 0.9 0.19 0.14 0.26 0.71 0.81 0.88 0.91 0.88 0.83
Cytoplasm 0.33 0.19 0.08 0.17 0.07 0.11 0.11 0.08 – 0.17 0.22 0.18 0.08 0.07 0.07 0.79 0.78 0.72 0.06 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0.08 0.07 0.05 0 0.08 – 0.09 0.09 0.11 0 0 0.05 0 0.09 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.09 0.26 0.12 0.07 0.13 0.18 0.2 – 0.11 0.17 0.1 0 0 0 0.08 0.07 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 39 125 131 79 106 131 166 160 – 129 72 139 53 61 33 5 25 26 9 17 11 5 19 25
Bud 14 29 11 16 20 14 11 21 – 14 26 15 3 5 1 5 9 13 3 12 5 4 14 20
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 3 0 7 12 4 1 8 22
Bud Site 0 4 1 0 1 0 0 0 – 0 0 0 0 0 0 0 1 0 – – – – – –
Cell Periphery 80 283 292 174 244 242 250 261 – 202 156 268 257 406 349 38 53 82 108 320 349 274 508 557
Cytoplasm 44 69 27 39 23 33 37 31 – 49 49 65 23 33 26 161 289 227 8 13 3 1 2 3
Endoplasmic Reticulum 2 7 5 17 21 16 14 30 – 24 21 40 6 17 20 8 35 8 5 7 11 2 6 12
Endosome 1 0 1 0 0 1 0 0 – 0 0 0 1 0 0 0 0 4 1 1 0 0 0 2
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 0 3 7 0 0 0 0 0 0 1 0 2
Mitochondria 21 31 87 28 23 37 59 79 – 32 38 34 0 0 2 16 25 8 6 4 7 2 6 10
Nucleus 0 2 1 0 2 0 0 2 – 0 1 1 0 1 0 6 3 1 0 0 0 0 0 1
Nuclear Periphery 2 1 0 0 0 0 6 14 – 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 2 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 4 0 3 1 3 0 1 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 3 8 2 1 2 1 1 1 – 0 0 0 0 0 0 1 8 4 0 0 0 0 0 0
Vac/Vac Membrane 1 0 3 0 1 0 1 3 – 0 0 0 0 1 2 1 17 4 1 5 4 3 10 11
Unique Cell Count 133 363 338 225 307 292 331 389 282 224 356 277 444 389 203 372 316 152 398 399 302 578 674
Labelled Cell Count 212 559 564 355 446 477 546 602 452 363 563 343 527 441 242 469 377 152 398 399 302 578 674


Bud, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 10.3 12.0 12.1 16.5 15.2 15.3 16.3 – 16.5 16.1 17.3 21.8 28.9 31.8 5.6 6.0 7.4 15.6 14.1 13.4
Std Deviation (1e-4) 2.5 3.5 3.8 4.0 4.5 3.7 3.7 3.8 – 3.9 3.9 4.0 5.9 8.5 10.7 1.9 2.3 2.5 4.3 4.5 5.0
Intensity Change (Log2) – – – 0.02 0.46 0.34 0.35 0.44 – 0.46 0.42 0.53 0.86 1.27 1.41 -1.11 -1.01 -0.7 0.38 0.23 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.9 -1.1 1.6 3.0 0.7 – 1.8 -1.6 0.1 -5.3 -8.1 -9.7 -9.4 -10.3 -9.1
Bud 2.1 1.9 1.0 0 1.4 – 1.1 3.9 0.7 -1.8 -2.1 -3.2 -0.5 -0.7 0.6
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery -2.8 -2.3 -1.2 -3.6 -6.1 – -4.5 -4.8 -3.7 2.5 2.3 1.4 -15.6 -19.2 -15.6
Cytoplasm 3.4 -0.2 1.4 1.4 0 – 3.5 4.7 4.0 0.1 -0.3 -0.7 16.9 18.7 16.7
Endoplasmic Reticulum 3.6 3.5 2.8 2.1 3.9 – 4.1 4.4 5.2 0 2.0 2.7 0 4.6 1.0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria -3.8 -6.2 -4.1 -2.5 -1.7 – -4.5 -2.4 -5.6 -9.1 -11.3 -10.3 -5.1 -6.9 -8.4
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 3.5 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 3.0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.9094 27.4023 24.267 21.9347 23.337 25.6494 8.9118 14.7301 14.7912 16.0731 12.5265 16.0829 15.3634 18.9166 18.0087 18.5716 18.2617 18.7255
Actin 0.018 0.0004 0.0007 0.0023 0.0016 0.0015 0.1133 0.0001 0.0221 0.0002 0.0228 0 0.001 0.0002 0.002 0.0003 0 0.0019
Bud 0.0018 0.0004 0.0004 0.0004 0.0003 0.0002 0.0024 0.0001 0.001 0.0003 0.0016 0.0002 0.0002 0.0001 0.0002 0.0005 0.0001 0.0002
Bud Neck 0.0015 0.0006 0.0006 0.0011 0.0005 0.0003 0.0045 0.0011 0.0023 0.0029 0.0012 0.0003 0.0005 0.0003 0.0009 0.0015 0.0004 0.0014
Bud Periphery 0.0154 0.0013 0.0045 0.0029 0.0039 0.0028 0.0059 0.0008 0.0058 0.006 0.0058 0.0054 0.0019 0.0009 0.0022 0.0156 0.0027 0.0045
Bud Site 0.0087 0.0053 0.0032 0.003 0.0008 0.0003 0.0402 0.0631 0.0062 0.0006 0.0045 0.0001 0.0032 0.003 0.0063 0.0011 0.0002 0.0003
Cell Periphery 0.8315 0.9189 0.917 0.867 0.8825 0.9286 0.578 0.8527 0.8237 0.8714 0.8613 0.9486 0.8802 0.9064 0.9279 0.8962 0.9595 0.9425
Cytoplasm 0.0076 0.0283 0.012 0.0116 0.0035 0.0053 0.0296 0.048 0.0182 0.0061 0.0086 0.0062 0.0391 0.0439 0.02 0.0245 0.0038 0.0055
Cytoplasmic Foci 0.0019 0.0008 0.0003 0.0119 0.0001 0.0005 0.0138 0 0.014 0 0.0122 0 0.0078 0 0.0001 0 0 0.0001
Eisosomes 0.0038 0.0004 0.0011 0.0012 0.0125 0.0005 0.0023 0.0009 0.0037 0.0013 0.0003 0.0002 0.0013 0.0006 0.0008 0.001 0.0001 0.0008
Endoplasmic Reticulum 0.0227 0.0117 0.0187 0.0437 0.0251 0.0246 0.0308 0.0246 0.0134 0.0891 0.0195 0.0174 0.0217 0.021 0.0153 0.0284 0.015 0.0219
Endosome 0.008 0.0028 0.0025 0.0014 0.0003 0.0002 0.0493 0 0.0055 0 0.0086 0 0.0008 0 0 0 0 0.0001
Golgi 0.0016 0.0012 0.0005 0.0005 0.0004 0.0001 0.0137 0 0.0023 0.0001 0.0123 0 0.0002 0.0001 0.0001 0 0 0.0003
Lipid Particles 0.0018 0.0016 0.0024 0.004 0.0009 0.0006 0.0202 0.0006 0.0061 0.0011 0.0037 0.0005 0.0011 0.0012 0.0004 0.0007 0.0002 0.0016
Mitochondria 0.0125 0.0017 0.0009 0.0053 0.0243 0.0017 0.0107 0 0.0126 0.0001 0.0098 0 0.0001 0 0 0.0001 0 0
None 0.0029 0.0001 0.0003 0.0068 0.0031 0.0009 0.0248 0.0001 0.012 0.0001 0.0002 0 0.0029 0.0001 0.0001 0.0001 0 0.0001
Nuclear Periphery 0.0078 0.0029 0.0052 0.0036 0.0044 0.0026 0.0223 0.0012 0.0108 0.0039 0.0026 0.0012 0.0091 0.0035 0.0028 0.0031 0.0014 0.0015
Nucleolus 0.0017 0.0003 0.0004 0.0014 0.001 0.0003 0.0015 0 0.0005 0.0001 0.0001 0 0.0001 0 0 0.0001 0 0.0001
Nucleus 0.002 0.0004 0.0006 0.0014 0.0011 0.0007 0.0042 0.0001 0.0016 0.0002 0.0003 0.0001 0.0032 0.0001 0.0002 0.0003 0.0001 0.0001
Peroxisomes 0.0006 0.0006 0.0001 0.0005 0.0002 0 0.0038 0 0.0047 0 0.0053 0 0.0002 0 0 0 0 0
Punctate Nuclear 0.0025 0.0002 0.0007 0.0076 0 0.0013 0.008 0 0.005 0 0.0004 0 0.0024 0 0 0 0 0
Vacuole 0.0438 0.0197 0.0275 0.0218 0.0326 0.0268 0.017 0.0063 0.0272 0.0164 0.0184 0.0194 0.023 0.0185 0.0206 0.0263 0.0163 0.0169
Vacuole Periphery 0.002 0.0003 0.0005 0.0006 0.0009 0.0002 0.0038 0 0.0014 0.0002 0.0004 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.2446 6.9744 11.4596 21.2281 7.2253 20.6078 14.6073 19.7178 24.4892 8.2661
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane
Localization
Cell Percentages cell periphery (67%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-3

Tpo4

Tpo4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tpo4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available