Standard name
Human Ortholog
Description Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.5 0.49 0.47 0.41 0.56 0.54 0.47 0.52 – 0.46 0.42 0.38 0.5 0.5 0.53 – – – 0.06 0.16 0.05 0.09 0 0.06
Endoplasmic Reticulum 0.12 0.4 0.17 0.13 0.05 0.05 0 0 – 0 0 0.05 0.5 0.52 0.44 – – – 0.71 0.53 0.73 0.55 0.54 0.48
Endosome 0.08 0 0.15 0.09 0.08 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0.2 0.1 0.4 0.5 0.45 – 0.57 0.6 0.63 0 0 0 – – – 0 0 0 0.1 0.09 0.05
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0.07 0.05 0.05 0.08 0.06 0.05
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.45 0.15 0.3 0.27 0.32 0.16 0.19 0.12 – 0.12 0.09 0.1 0 0.05 0.1 – – – 0.1 0.15 0.06 0.11 0.18 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 6 1 1 0 0 0 0 – 0 1 0 0 0 0 – – – 1 6 5 2 2 3
Bud 0 4 2 2 5 3 14 24 – 17 18 10 1 1 0 – – – 1 1 2 1 3 4
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 3 4 0 1 1
Bud Site 0 0 0 0 0 0 0 0 – 1 3 1 0 0 0 – – – – – – – – –
Cell Periphery 0 0 1 1 1 2 2 1 – 3 6 4 3 3 6 – – – 0 0 0 0 0 0
Cytoplasm 214 201 207 133 199 332 375 399 – 345 327 281 104 104 109 – – – 30 81 27 22 13 23
Endoplasmic Reticulum 52 164 75 43 17 29 7 5 – 20 28 38 102 109 91 – – – 362 272 421 140 173 190
Endosome 34 2 66 30 28 14 12 27 – 6 3 5 3 5 1 – – – 9 15 16 5 12 17
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 2 2 4 1 4 2
Mitochondria 1 8 11 66 36 244 405 347 – 432 465 464 0 0 0 – – – 8 17 22 26 29 19
Nucleus 0 1 1 1 4 2 5 9 – 9 12 12 1 1 1 – – – 0 0 0 0 0 2
Nuclear Periphery 0 0 1 11 0 3 4 4 – 11 6 14 1 1 0 – – – 35 23 29 21 17 17
Nucleolus 1 0 2 1 1 0 1 2 – 3 0 0 0 0 0 – – – 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 1 1 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 2 1 0 0 0 0 0 – 1 1 1 0 0 0 – – – 0 1 0 0 0 2
Vac/Vac Membrane 193 63 133 89 114 97 155 94 – 91 69 74 9 10 20 – – – 48 78 35 26 59 102
Unique Cell Count 430 410 444 326 357 611 802 764 756 778 740 206 209 206 507 510 578 254 324 394
Labelled Cell Count 496 451 501 378 405 726 981 913 939 939 904 224 234 228 507 510 578 254 324 394


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 6.3 5.8 5.2 5.1 4.2 3.8 4.1 – 3.6 3.7 3.8 8.2 8.2 8.5 – – – 7.6 8.3 8.6
Std Deviation (1e-4) 1.1 0.9 1.1 1.1 1.3 1.2 1.0 1.0 – 0.7 0.7 0.8 0.9 0.9 1.2 – – – 1.4 1.6 1.5
Intensity Change (Log2) – – – -0.17 -0.2 -0.47 -0.61 -0.5 – -0.71 -0.67 -0.62 0.48 0.48 0.54 – – – 0.37 0.5 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Cytoplasm -1.6 2.6 2.5 0 1.9 – -0.3 -1.6 -2.9 0.9 0.7 1.5 – – –
Endoplasmic Reticulum -1.4 -5.4 0 0 0 – 0 0 0 8.7 9.3 7.4 – – –
Endosome -2.4 -3.1 0 0 0 – 0 0 0 -5.2 -4.8 -5.6 – – –
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Mitochondria 8.1 4.6 0 0 0 – 0 0 0 0 0 0 – – –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nuclear Periphery 3.5 0 0 0 0 – 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Vacuole -0.8 0.6 -5.5 -4.3 -7.6 – -7.7 -9.5 -8.8 -7.3 -7.3 -5.7 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5185 4.4388 4.4189 4.6558 3.8484 4.4045 5.1914 5.4604 5.4961 5.0653 4.2051 5.2322 5.7289 5.7284 5.411 5.7702 5.2029 5.3446
Actin 0.0289 0.0029 0.0088 0.0004 0.0011 0.0027 0.022 0.0002 0.0158 0.0188 0.0158 0.0065 0.0369 0.0005 0.0106 0.0256 0.0047 0.0032
Bud 0.0003 0.0002 0.0001 0.0001 0.0012 0.0002 0.0005 0.0013 0.0015 0.0007 0.0006 0.0007 0.0009 0.0001 0.0002 0.0003 0.0002 0.0002
Bud Neck 0.0007 0.0004 0.0003 0.0005 0.0006 0.0028 0.0014 0.0001 0.0006 0.001 0.0002 0.0008 0.0022 0.0004 0.0008 0.001 0.001 0.0005
Bud Periphery 0.0007 0.0001 0.0001 0.0001 0.0004 0.0002 0.0007 0.0004 0.0003 0.0007 0.0007 0.0003 0.0012 0.0001 0.0002 0.0007 0.0005 0.0002
Bud Site 0.0003 0.0029 0.0004 0.0003 0.0009 0.0002 0.0031 0.0042 0.004 0.005 0.0007 0.0003 0.0099 0.0084 0.0024 0.0009 0.0002 0.0001
Cell Periphery 0.0002 0.0004 0.0002 0.0002 0.0002 0.0001 0.0006 0.0004 0.0004 0.0004 0.0002 0.0002 0.0006 0.0006 0.0004 0.0004 0.0003 0.0001
Cytoplasm 0.1225 0.3184 0.2407 0.1851 0.2974 0.2585 0.1231 0.2146 0.2001 0.1112 0.2579 0.1895 0.1067 0.3686 0.1666 0.263 0.3641 0.1719
Cytoplasmic Foci 0.0172 0.0092 0.0048 0.0024 0.0242 0.0062 0.04 0.0044 0.0134 0.0241 0.0095 0.009 0.0271 0.0098 0.0158 0.0098 0.0077 0.0075
Eisosomes 0.0002 0.0001 0 0 0 0 0.0005 0 0.0002 0.0001 0.0001 0 0.0011 0 0 0.0005 0.0001 0.0001
Endoplasmic Reticulum 0.5554 0.4508 0.5875 0.6676 0.3965 0.5377 0.4331 0.6215 0.5349 0.5858 0.5349 0.6096 0.5202 0.4843 0.6314 0.4555 0.4063 0.6164
Endosome 0.073 0.0491 0.0266 0.029 0.0738 0.0337 0.0895 0.0239 0.0316 0.0367 0.0328 0.0318 0.0613 0.0239 0.0606 0.0402 0.0417 0.0399
Golgi 0.0038 0.0013 0.0009 0.0004 0.0011 0.0029 0.0204 0.0004 0.0085 0.0238 0.0059 0.0034 0.0201 0.0005 0.0087 0.0224 0.0357 0.0087
Lipid Particles 0.0069 0.0041 0.0024 0.0011 0.0035 0.0045 0.0535 0.0032 0.0091 0.0134 0.0041 0.0091 0.0377 0.004 0.0073 0.0132 0.0093 0.0105
Mitochondria 0.0015 0.0005 0.0004 0.0006 0.0006 0.0011 0.01 0.0002 0.0092 0.0034 0.0018 0.001 0.0083 0.0003 0.0018 0.0445 0.048 0.0104
None 0.0097 0.021 0.0256 0.0026 0.0185 0.0189 0.0417 0.0451 0.0754 0.0586 0.0387 0.0256 0.0087 0.0179 0.0139 0.0723 0.0187 0.035
Nuclear Periphery 0.1316 0.0873 0.0746 0.0645 0.107 0.0756 0.0736 0.0409 0.0555 0.0466 0.0648 0.0691 0.09 0.0526 0.0536 0.0295 0.0318 0.0608
Nucleolus 0.0003 0.0008 0.0001 0.0003 0.0009 0.0002 0.0042 0.0008 0.0004 0.0015 0.0001 0.0007 0.0006 0.0002 0.0001 0.0002 0.0001 0.0001
Nucleus 0.0186 0.0197 0.011 0.0234 0.0259 0.0345 0.0275 0.0094 0.0148 0.0055 0.0089 0.0187 0.0161 0.0135 0.0059 0.0049 0.0116 0.0131
Peroxisomes 0.0022 0.0001 0.0006 0 0.0002 0.0006 0.0166 0 0.0099 0.0084 0.0044 0.0014 0.0208 0.0001 0.0008 0.0042 0.0009 0.0038
Punctate Nuclear 0.0097 0.0025 0.0011 0.0021 0.0154 0.0026 0.0158 0.0039 0.0026 0.0397 0.0014 0.0051 0.0034 0.0018 0.0008 0.0007 0.0012 0.001
Vacuole 0.0124 0.0255 0.0122 0.0166 0.0255 0.0142 0.0176 0.0237 0.0101 0.0128 0.0142 0.0147 0.0212 0.0112 0.016 0.0076 0.013 0.0139
Vacuole Periphery 0.0039 0.0027 0.0018 0.0026 0.0049 0.0027 0.0046 0.0016 0.0018 0.0019 0.0023 0.0024 0.005 0.0012 0.0019 0.0027 0.0028 0.0026

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.7247 11.3838 16.3977 22.2423 16.3489 15.4726 17.8859 23.0773 21.7467 23.2977
Translational Efficiency 0.3384 0.462 0.4105 0.3204 0.3567 0.4751 0.3982 0.2887 0.3142 0.3117

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
Localization
Cell Percentages ER (61%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Ypk9

Ypk9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypk9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available