Standard name | |
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Human Ortholog | |
Description | Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 6 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 1 | 6 | 5 | 2 | 2 | 3 |
Bud | 0 | 4 | 2 | 2 | 5 | 3 | 14 | 24 | – | 17 | 18 | 10 | 1 | 1 | 0 | – | – | – | 1 | 1 | 2 | 1 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 3 | 4 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 3 | 1 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 1 | 1 | 2 | 2 | 1 | – | 3 | 6 | 4 | 3 | 3 | 6 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 214 | 201 | 207 | 133 | 199 | 332 | 375 | 399 | – | 345 | 327 | 281 | 104 | 104 | 109 | – | – | – | 30 | 81 | 27 | 22 | 13 | 23 |
Endoplasmic Reticulum | 52 | 164 | 75 | 43 | 17 | 29 | 7 | 5 | – | 20 | 28 | 38 | 102 | 109 | 91 | – | – | – | 362 | 272 | 421 | 140 | 173 | 190 |
Endosome | 34 | 2 | 66 | 30 | 28 | 14 | 12 | 27 | – | 6 | 3 | 5 | 3 | 5 | 1 | – | – | – | 9 | 15 | 16 | 5 | 12 | 17 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 2 | 2 | 4 | 1 | 4 | 2 |
Mitochondria | 1 | 8 | 11 | 66 | 36 | 244 | 405 | 347 | – | 432 | 465 | 464 | 0 | 0 | 0 | – | – | – | 8 | 17 | 22 | 26 | 29 | 19 |
Nucleus | 0 | 1 | 1 | 1 | 4 | 2 | 5 | 9 | – | 9 | 12 | 12 | 1 | 1 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 2 |
Nuclear Periphery | 0 | 0 | 1 | 11 | 0 | 3 | 4 | 4 | – | 11 | 6 | 14 | 1 | 1 | 0 | – | – | – | 35 | 23 | 29 | 21 | 17 | 17 |
Nucleolus | 1 | 0 | 2 | 1 | 1 | 0 | 1 | 2 | – | 3 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 1 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 0 | 2 |
Vac/Vac Membrane | 193 | 63 | 133 | 89 | 114 | 97 | 155 | 94 | – | 91 | 69 | 74 | 9 | 10 | 20 | – | – | – | 48 | 78 | 35 | 26 | 59 | 102 |
Unique Cell Count | 430 | 410 | 444 | 326 | 357 | 611 | 802 | 764 | 756 | 778 | 740 | 206 | 209 | 206 | 507 | 510 | 578 | 254 | 324 | 394 | ||||
Labelled Cell Count | 496 | 451 | 501 | 378 | 405 | 726 | 981 | 913 | 939 | 939 | 904 | 224 | 234 | 228 | 507 | 510 | 578 | 254 | 324 | 394 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 6.3 | 5.8 | 5.2 | 5.1 | 4.2 | 3.8 | 4.1 | – | 3.6 | 3.7 | 3.8 | 8.2 | 8.2 | 8.5 | – | – | – | 7.6 | 8.3 | 8.6 |
Std Deviation (1e-4) | 1.1 | 0.9 | 1.1 | 1.1 | 1.3 | 1.2 | 1.0 | 1.0 | – | 0.7 | 0.7 | 0.8 | 0.9 | 0.9 | 1.2 | – | – | – | 1.4 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.2 | -0.47 | -0.61 | -0.5 | – | -0.71 | -0.67 | -0.62 | 0.48 | 0.48 | 0.54 | – | – | – | 0.37 | 0.5 | 0.55 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | -1.6 | 2.6 | 2.5 | 0 | 1.9 | – | -0.3 | -1.6 | -2.9 | 0.9 | 0.7 | 1.5 | – | – | – |
Endoplasmic Reticulum | -1.4 | -5.4 | 0 | 0 | 0 | – | 0 | 0 | 0 | 8.7 | 9.3 | 7.4 | – | – | – |
Endosome | -2.4 | -3.1 | 0 | 0 | 0 | – | 0 | 0 | 0 | -5.2 | -4.8 | -5.6 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 8.1 | 4.6 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 3.5 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | -0.8 | 0.6 | -5.5 | -4.3 | -7.6 | – | -7.7 | -9.5 | -8.8 | -7.3 | -7.3 | -5.7 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.5185 | 4.4388 | 4.4189 | 4.6558 | 3.8484 | 4.4045 | 5.1914 | 5.4604 | 5.4961 | 5.0653 | 4.2051 | 5.2322 | 5.7289 | 5.7284 | 5.411 | 5.7702 | 5.2029 | 5.3446 |
Actin | 0.0289 | 0.0029 | 0.0088 | 0.0004 | 0.0011 | 0.0027 | 0.022 | 0.0002 | 0.0158 | 0.0188 | 0.0158 | 0.0065 | 0.0369 | 0.0005 | 0.0106 | 0.0256 | 0.0047 | 0.0032 |
Bud | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0012 | 0.0002 | 0.0005 | 0.0013 | 0.0015 | 0.0007 | 0.0006 | 0.0007 | 0.0009 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0002 |
Bud Neck | 0.0007 | 0.0004 | 0.0003 | 0.0005 | 0.0006 | 0.0028 | 0.0014 | 0.0001 | 0.0006 | 0.001 | 0.0002 | 0.0008 | 0.0022 | 0.0004 | 0.0008 | 0.001 | 0.001 | 0.0005 |
Bud Periphery | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0007 | 0.0004 | 0.0003 | 0.0007 | 0.0007 | 0.0003 | 0.0012 | 0.0001 | 0.0002 | 0.0007 | 0.0005 | 0.0002 |
Bud Site | 0.0003 | 0.0029 | 0.0004 | 0.0003 | 0.0009 | 0.0002 | 0.0031 | 0.0042 | 0.004 | 0.005 | 0.0007 | 0.0003 | 0.0099 | 0.0084 | 0.0024 | 0.0009 | 0.0002 | 0.0001 |
Cell Periphery | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0006 | 0.0004 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0006 | 0.0006 | 0.0004 | 0.0004 | 0.0003 | 0.0001 |
Cytoplasm | 0.1225 | 0.3184 | 0.2407 | 0.1851 | 0.2974 | 0.2585 | 0.1231 | 0.2146 | 0.2001 | 0.1112 | 0.2579 | 0.1895 | 0.1067 | 0.3686 | 0.1666 | 0.263 | 0.3641 | 0.1719 |
Cytoplasmic Foci | 0.0172 | 0.0092 | 0.0048 | 0.0024 | 0.0242 | 0.0062 | 0.04 | 0.0044 | 0.0134 | 0.0241 | 0.0095 | 0.009 | 0.0271 | 0.0098 | 0.0158 | 0.0098 | 0.0077 | 0.0075 |
Eisosomes | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0011 | 0 | 0 | 0.0005 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.5554 | 0.4508 | 0.5875 | 0.6676 | 0.3965 | 0.5377 | 0.4331 | 0.6215 | 0.5349 | 0.5858 | 0.5349 | 0.6096 | 0.5202 | 0.4843 | 0.6314 | 0.4555 | 0.4063 | 0.6164 |
Endosome | 0.073 | 0.0491 | 0.0266 | 0.029 | 0.0738 | 0.0337 | 0.0895 | 0.0239 | 0.0316 | 0.0367 | 0.0328 | 0.0318 | 0.0613 | 0.0239 | 0.0606 | 0.0402 | 0.0417 | 0.0399 |
Golgi | 0.0038 | 0.0013 | 0.0009 | 0.0004 | 0.0011 | 0.0029 | 0.0204 | 0.0004 | 0.0085 | 0.0238 | 0.0059 | 0.0034 | 0.0201 | 0.0005 | 0.0087 | 0.0224 | 0.0357 | 0.0087 |
Lipid Particles | 0.0069 | 0.0041 | 0.0024 | 0.0011 | 0.0035 | 0.0045 | 0.0535 | 0.0032 | 0.0091 | 0.0134 | 0.0041 | 0.0091 | 0.0377 | 0.004 | 0.0073 | 0.0132 | 0.0093 | 0.0105 |
Mitochondria | 0.0015 | 0.0005 | 0.0004 | 0.0006 | 0.0006 | 0.0011 | 0.01 | 0.0002 | 0.0092 | 0.0034 | 0.0018 | 0.001 | 0.0083 | 0.0003 | 0.0018 | 0.0445 | 0.048 | 0.0104 |
None | 0.0097 | 0.021 | 0.0256 | 0.0026 | 0.0185 | 0.0189 | 0.0417 | 0.0451 | 0.0754 | 0.0586 | 0.0387 | 0.0256 | 0.0087 | 0.0179 | 0.0139 | 0.0723 | 0.0187 | 0.035 |
Nuclear Periphery | 0.1316 | 0.0873 | 0.0746 | 0.0645 | 0.107 | 0.0756 | 0.0736 | 0.0409 | 0.0555 | 0.0466 | 0.0648 | 0.0691 | 0.09 | 0.0526 | 0.0536 | 0.0295 | 0.0318 | 0.0608 |
Nucleolus | 0.0003 | 0.0008 | 0.0001 | 0.0003 | 0.0009 | 0.0002 | 0.0042 | 0.0008 | 0.0004 | 0.0015 | 0.0001 | 0.0007 | 0.0006 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Nucleus | 0.0186 | 0.0197 | 0.011 | 0.0234 | 0.0259 | 0.0345 | 0.0275 | 0.0094 | 0.0148 | 0.0055 | 0.0089 | 0.0187 | 0.0161 | 0.0135 | 0.0059 | 0.0049 | 0.0116 | 0.0131 |
Peroxisomes | 0.0022 | 0.0001 | 0.0006 | 0 | 0.0002 | 0.0006 | 0.0166 | 0 | 0.0099 | 0.0084 | 0.0044 | 0.0014 | 0.0208 | 0.0001 | 0.0008 | 0.0042 | 0.0009 | 0.0038 |
Punctate Nuclear | 0.0097 | 0.0025 | 0.0011 | 0.0021 | 0.0154 | 0.0026 | 0.0158 | 0.0039 | 0.0026 | 0.0397 | 0.0014 | 0.0051 | 0.0034 | 0.0018 | 0.0008 | 0.0007 | 0.0012 | 0.001 |
Vacuole | 0.0124 | 0.0255 | 0.0122 | 0.0166 | 0.0255 | 0.0142 | 0.0176 | 0.0237 | 0.0101 | 0.0128 | 0.0142 | 0.0147 | 0.0212 | 0.0112 | 0.016 | 0.0076 | 0.013 | 0.0139 |
Vacuole Periphery | 0.0039 | 0.0027 | 0.0018 | 0.0026 | 0.0049 | 0.0027 | 0.0046 | 0.0016 | 0.0018 | 0.0019 | 0.0023 | 0.0024 | 0.005 | 0.0012 | 0.0019 | 0.0027 | 0.0028 | 0.0026 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 20.7247 | 11.3838 | 16.3977 | 22.2423 | 16.3489 | 15.4726 | 17.8859 | 23.0773 | 21.7467 | 23.2977 |
Translational Efficiency | 0.3384 | 0.462 | 0.4105 | 0.3204 | 0.3567 | 0.4751 | 0.3982 | 0.2887 | 0.3142 | 0.3117 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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