Standard name
Human Ortholog
Description Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.09 0.28 0.1 0.14 0 0 0 0 – 0 0 0 0 0 0.11 – 0 0 0.05 0 0.06 0.07 0.06
Bud 0 0 0.07 0.07 0.05 0 0.06 0.05 0.05 – 0.08 0.06 0.05 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 – 0.05 0 0 0 0 0 0
Cytoplasm 0.2 0.29 0.37 0.22 0.29 0.16 0.14 0.17 0.15 – 0.1 0.14 0.12 0.13 0.22 0.47 – 0.5 0 0.09 0 0 0 0
Endoplasmic Reticulum 0.07 0.11 0.14 0 0.05 0.06 0 0 0 – 0 0 0.08 0 0.07 0.11 – 0.05 0 0 0 0 0 0
Endosome 0.15 0 0.09 0.22 0.24 0.14 0.14 0.16 0 – 0 0.13 0.16 0.39 0.19 0.21 – 0.2 0.35 0.25 0.25 0.26 0.23 0.2
Golgi 0.07 0.05 0 0 0 0 0 0 0 – 0 0 0.09 0.09 0.09 0 – 0.05 0.22 0.19 0.11 0.17 0.29 0.39
Mitochondria 0.6 0.6 0.08 0.4 0.23 0.64 0.62 0.49 0.76 – 0.89 0.66 0.63 0.37 0.42 0 – 0.2 0.21 0.28 0.42 0.32 0.25 0.19
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0.08 0 0 – 0 0.05 0 0 0.07 0.05 0.07
SpindlePole 0 0 0.1 0.11 0.16 0.07 0.13 0.19 0.05 – 0 0.09 0 0 0 0.11 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0.05 0.05 0.08 0.05 – 0.05 0.07 0 0.09 0.07 0.16 – 0.15 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 6 29 16 32 5 18 12 5 – 1 3 7 1 3 2 – 0 7 5 9 10 25 21
Bud 6 1 7 12 11 12 28 19 20 – 28 26 11 12 6 0 – 0 0 0 1 2 4 1
Bud Neck 0 0 3 1 0 0 2 4 1 – 0 0 0 0 0 0 – 0 0 1 1 0 0 1
Bud Site 0 0 0 0 0 0 8 0 4 – 5 7 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 1 0 2 1 2 0 1 – 0 2 0 1 0 1 – 1 0 0 0 0 0 0
Cytoplasm 35 19 38 35 65 50 64 67 53 – 37 58 28 35 69 9 – 10 6 9 8 3 4 2
Endoplasmic Reticulum 12 7 14 5 12 17 11 2 11 – 3 1 18 3 22 2 – 1 4 0 0 1 8 6
Endosome 27 0 9 35 55 43 63 62 13 – 12 52 36 106 59 4 – 4 74 24 55 45 83 73
Golgi 12 3 1 7 3 0 0 1 1 – 0 0 21 24 28 0 – 1 46 18 24 29 103 140
Mitochondria 107 39 8 64 52 198 281 193 277 – 321 269 141 100 129 0 – 4 45 27 94 55 87 70
Nucleus 0 0 1 2 0 2 4 6 4 – 3 2 0 2 0 0 – 0 0 1 0 0 1 0
Nuclear Periphery 1 1 0 0 0 0 0 1 0 – 0 0 1 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 3 0 1 1 3 3 – 2 0 1 1 3 0 – 0 0 0 0 0 0 1
Peroxisomes 2 0 0 4 2 6 7 4 1 – 2 12 6 22 4 0 – 0 11 1 8 11 18 25
SpindlePole 1 0 10 18 36 22 59 75 19 – 13 37 1 8 6 2 – 0 8 1 7 3 5 4
Vac/Vac Membrane 8 1 4 6 9 16 22 31 20 – 18 29 9 24 23 3 – 3 4 5 6 6 7 8
Unique Cell Count 177 65 103 161 228 308 453 392 364 362 406 224 272 308 19 20 212 98 224 175 355 364
Labelled Cell Count 217 77 125 208 279 373 570 480 433 445 498 280 339 352 23 24 212 98 224 175 355 364


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.2 5.9 5.6 6.1 5.0 5.0 5.3 4.5 – 4.1 4.7 6.1 6.4 6.5 8.1 – 7.1 5.3 5.6 6.1
Std Deviation (1e-4) 0.6 0.6 0.7 0.9 1.0 1.2 0.8 1.0 1.0 – 0.7 0.9 0.9 0.9 1.1 1.5 – 1.4 0.6 1.1 1.3
Intensity Change (Log2) – – – -0.09 0.04 -0.26 -0.26 -0.16 -0.42 – -0.53 -0.33 0.04 0.11 0.12 0.45 – 0.25 -0.17 -0.09 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -3.8 -3.1 -8.5 -8.0 -8.2 0 – 0 0 -6.7 -8.9 -8.9 0 – 0
Bud 0.2 -0.7 0 -0.2 -0.8 0 – 0 0 -0.7 -0.9 0 0 – 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0
Cytoplasm -2.7 -1.5 -4.4 -5.4 -4.4 -5.1 – -6.5 -5.2 -5.1 -5.2 -2.9 0.9 – 1.1
Endoplasmic Reticulum -3.2 -2.6 -2.7 0 0 0 – 0 0 -1.6 0 -2.0 0 – 0
Endosome 2.8 3.3 1.4 1.4 1.8 0 – 0 0 1.8 5.7 2.5 0 – 0
Golgi 0 0 0 0 0 0 – 0 0 2.8 2.7 2.8 0 – 0
Mitochondria 5.7 3.3 9.9 9.9 7.6 0 – 0 0 9.3 5.5 6.4 0 – 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 3.0 0 0 – 0
SpindlePole 0.4 1.5 -0.8 0.9 2.3 0 – 0 0 0 0 0 0 – 0
Vacuole 0 0 0.5 0 1.4 0.7 – 0 1.2 0 1.6 1.3 0 – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7935 1.5546 0.9437 0.9854 0.6973 1.4198 2.0032 2.4473 2.2382 1.9971 2.2596 2.1701 2.5498 2.8894 2.3414 1.9868 2.1538 2.5319
Actin 0.0255 0.0125 0.0116 0.0085 0.0039 0.0199 0.0432 0.0088 0.0264 0.0329 0.0274 0.0079 0.023 0.0082 0.0261 0.0018 0.0479 0.0081
Bud 0.0007 0.0008 0.0004 0.0003 0.0003 0.0003 0.0007 0.0004 0.0004 0.0012 0.0009 0.0002 0.0006 0.0004 0.0004 0.0002 0.0002 0.0002
Bud Neck 0.0082 0.0007 0.0018 0.0008 0.0024 0.0009 0.0024 0.0005 0.0008 0.0008 0.0008 0.0011 0.0006 0.0006 0.0008 0.0004 0.0005 0.0028
Bud Periphery 0.0008 0.0004 0.0002 0.0003 0.0004 0.0005 0.0007 0.0002 0.0004 0.002 0.0006 0.0004 0.0008 0.0002 0.0002 0.0001 0.0002 0.0001
Bud Site 0.0111 0.0054 0.0014 0.0011 0.0059 0.0004 0.0129 0.0093 0.0025 0.001 0.0013 0.0003 0.0029 0.0139 0.0029 0.0005 0.0007 0.0003
Cell Periphery 0.0007 0.0001 0.0001 0.0001 0.0005 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0 0 0.0001
Cytoplasm 0.01 0.0412 0.031 0.008 0.0066 0.0142 0.0098 0.0205 0.0153 0.0084 0.0102 0.0074 0.0355 0.0358 0.0196 0.0132 0.0083 0.0285
Cytoplasmic Foci 0.1836 0.2368 0.2069 0.1611 0.2014 0.1385 0.2302 0.2707 0.2171 0.2088 0.2336 0.1855 0.2108 0.2441 0.208 0.2273 0.2282 0.1787
Eisosomes 0.0007 0.0001 0.0002 0 0.0003 0.0002 0.0003 0 0.0002 0 0.0001 0 0.0002 0.0001 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0028 0.0021 0.001 0.0018 0.0011 0.004 0.0032 0.0009 0.0013 0.0005 0.0005 0.0019 0.0051 0.0011 0.0026 0.0012 0.0009 0.0019
Endosome 0.3439 0.3797 0.338 0.5444 0.3114 0.352 0.3033 0.3326 0.2986 0.3667 0.2874 0.2855 0.341 0.3263 0.3549 0.5398 0.2901 0.3428
Golgi 0.2324 0.2365 0.2929 0.2308 0.3345 0.3612 0.2668 0.3072 0.3503 0.3158 0.3697 0.4258 0.2713 0.2999 0.3096 0.1679 0.3575 0.3873
Lipid Particles 0.0611 0.0132 0.0401 0.0092 0.0594 0.0151 0.0314 0.0044 0.008 0.0133 0.0139 0.011 0.0357 0.0042 0.0109 0.0057 0.0122 0.0058
Mitochondria 0.0213 0.0087 0.0119 0.0067 0.0093 0.0235 0.0169 0.0066 0.0206 0.0073 0.0102 0.0189 0.0068 0.0178 0.008 0.0021 0.004 0.0184
None 0.0244 0.018 0.0089 0.0007 0.0062 0.0008 0.0023 0.0046 0.0005 0.0005 0.0002 0.0002 0.0126 0.0146 0.0009 0.0002 0.0005 0.0012
Nuclear Periphery 0.006 0.0004 0.0007 0.0007 0.0021 0.0034 0.006 0.0002 0.0004 0.0002 0.0002 0.0015 0.0077 0.0004 0.0006 0.0005 0.0006 0.0007
Nucleolus 0.0028 0.0001 0.0001 0.0001 0.0116 0.0002 0.0004 0.0001 0.0001 0.0001 0 0 0.0005 0.0001 0.0001 0.0001 0 0
Nucleus 0.0049 0.0003 0.0002 0.0002 0.0049 0.0004 0.001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0018 0.0003 0.0002 0.0001 0.0001 0.0002
Peroxisomes 0.0407 0.0362 0.0437 0.0195 0.0212 0.0571 0.0615 0.0296 0.0533 0.0333 0.0406 0.0485 0.0338 0.0275 0.049 0.032 0.0444 0.0174
Punctate Nuclear 0.0098 0.0011 0.0007 0.0007 0.0105 0.0014 0.0031 0.0004 0.0003 0.0006 0.0004 0.0003 0.0043 0.0005 0.0019 0.0004 0.0012 0.0004
Vacuole 0.0057 0.0039 0.0058 0.0029 0.0044 0.0029 0.0019 0.0019 0.0019 0.004 0.0008 0.0014 0.0025 0.0028 0.002 0.0047 0.0012 0.0031
Vacuole Periphery 0.0026 0.0017 0.0023 0.0021 0.0017 0.0031 0.0016 0.001 0.0014 0.0023 0.0011 0.0019 0.0023 0.001 0.0014 0.0019 0.0011 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.233 14.0848 13.391 21.8743 20.4282 16.7314 22.8081 27.1834 23.6753 24.266
Translational Efficiency 0.5337 0.7247 0.7929 0.4868 0.4474 0.5771 0.4142 0.4556 0.5121 0.4847

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (20%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Bud7

Bud7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available