Standard name
Human Ortholog
Description Gamma-glutamyl phosphate reductase; catalyzes the second step in proline biosynthesis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.98 0.98 0.89 0.94 0.84 0.75 0.76 0.73 0.65 0.66 0.66 0.99 0.98 0.98 0.97 0.96 0.96 0.96 0.95 0.94 0.96 0.96 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.13 0.09 0.11 0.22 0.26 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.09 0 0.05 0.09 0.13 0.13 0.15 0.14 0.15 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 4 1 1 2 4 4 3 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 1 0 0 1 1 1 3 3 10 10 10 0 0 0 0 0 0 0 1 1 1 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 4 8 3 8 9 20 25 40 32 34 24 2 4 2 5 12 8 0 0 2 0 0 0
Cytoplasm 263 432 595 207 290 413 422 455 486 510 438 442 365 545 574 134 306 250 252 413 586 353 584 618
Endoplasmic Reticulum 1 4 4 3 3 11 19 18 34 27 12 14 2 0 5 4 9 3 0 2 5 0 2 5
Endosome 0 0 2 0 0 0 0 0 0 0 0 1 0 0 0 1 2 0 3 1 5 0 1 1
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 2 2 0 1
Mitochondria 2 2 2 3 6 54 75 57 72 175 174 155 0 0 0 2 2 1 2 1 6 1 1 0
Nucleus 1 1 4 0 0 3 10 7 8 12 6 3 2 0 4 2 1 5 0 4 2 4 5 1
Nuclear Periphery 0 0 0 0 0 2 0 0 0 0 4 0 0 0 0 0 0 0 0 0 2 0 0 1
Nucleolus 0 0 0 0 0 1 2 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Vac/Vac Membrane 0 3 2 22 11 25 50 79 86 118 91 103 1 25 7 2 13 9 0 2 3 2 8 3
Unique Cell Count 264 441 607 232 310 492 563 601 669 787 668 669 368 556 583 138 320 260 264 436 626 369 610 643
Labelled Cell Count 269 449 617 238 319 523 600 645 731 888 774 756 372 574 592 150 347 276 264 436 626 369 610 643


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.4 14.9 14.0 13.3 12.5 10.9 9.9 10.0 9.7 8.8 8.3 8.5 17.3 16.9 15.0 18.9 17.1 18.1 18.4 18.2 18.4
Std Deviation (1e-4) 2.4 2.8 2.8 2.5 2.3 2.2 2.0 2.0 1.9 1.8 2.0 2.0 3.4 3.2 3.6 5.7 4.4 5.5 3.2 3.6 3.5
Intensity Change (Log2) -0.08 -0.16 -0.36 -0.5 -0.49 -0.53 -0.67 -0.75 -0.73 0.31 0.26 0.1 0.43 0.28 0.36 0.39 0.37 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.4 0.7 2.5 3.0 4.4 3.0 3.8 2.6 0 -1.0 0 0 2.4 0
Cytoplasm -5.6 -3.5 -8.4 -11.7 -11.5 -12.6 -15.2 -14.8 -14.6 1.4 0 0.6 -0.7 -2.1 -1.6
Endoplasmic Reticulum 0 0 2.2 3.3 3.0 4.6 3.5 1.8 2.2 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 8.0 9.0 7.4 8.0 12.2 13.3 12.4 0 0 0 0 0 0
Nucleus 0 0 0 1.8 0.9 1.0 1.5 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 7.1 0 5.1 7.1 8.9 8.8 9.7 9.1 9.8 0 4.7 0 0 4.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.4898 23.0338 20.5319 18.9139 17.8319 20.5073 20.2566 27.2047 23.6405 23.0681 18.5266 23.2372 17.6667 23.4823 20.6031 18.476 19.0328 20.161
Actin 0.0048 0.0003 0.0023 0.0009 0.001 0.0005 0.0146 0.0002 0.0012 0.0008 0.0119 0.0005 0.021 0.0005 0.0065 0.0007 0.0004 0.0001
Bud 0.0004 0.0001 0.0005 0.0002 0.0019 0.0002 0.0009 0 0.0001 0.0002 0.0014 0.0001 0.0002 0.0001 0.0001 0.0002 0.0005 0.0001
Bud Neck 0.0004 0.0004 0.0008 0.0006 0.0005 0.0009 0.0015 0.0001 0.0004 0.0004 0.0013 0.0008 0.0004 0.0001 0.0004 0.0005 0.0006 0.0004
Bud Periphery 0.0002 0 0.0005 0.0001 0.0013 0.0001 0.001 0 0.0001 0.0001 0.0018 0 0.0001 0 0.0001 0.0001 0.0004 0
Bud Site 0.0005 0.0003 0.0007 0.0001 0.0062 0 0.0021 0 0.001 0.0001 0.0012 0 0.0007 0.0001 0.0002 0.0011 0.0005 0
Cell Periphery 0.0001 0 0.0002 0 0.0003 0 0.0002 0 0.0001 0 0.0003 0 0.0001 0 0 0.0001 0.0001 0
Cytoplasm 0.9133 0.9886 0.9432 0.955 0.8271 0.9623 0.8503 0.9733 0.9236 0.912 0.7133 0.936 0.8678 0.9837 0.9612 0.9181 0.9068 0.967
Cytoplasmic Foci 0.003 0.0004 0.0034 0.0009 0.0098 0.0018 0.0091 0.0003 0.0018 0.0071 0.0162 0.0009 0.013 0.0005 0.0016 0.0083 0.003 0.0006
Eisosomes 0 0 0 0 0.0001 0 0.0002 0 0 0 0.0001 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0037 0.0005 0.0016 0.002 0.0077 0.0032 0.0061 0.0033 0.0047 0.0011 0.0126 0.0011 0.0039 0.0004 0.0011 0.0035 0.0034 0.0007
Endosome 0.0108 0.0002 0.0022 0.0005 0.0309 0.002 0.0173 0.0001 0.0048 0.0067 0.0404 0.002 0.0079 0.0002 0.0009 0.0051 0.0102 0.0002
Golgi 0.0008 0 0.0005 0 0.0021 0.0001 0.0046 0 0.0011 0.0024 0.0059 0.001 0.0026 0 0.0003 0.0008 0.0004 0
Lipid Particles 0.0003 0 0.0021 0 0.0082 0 0.006 0 0.0044 0.0003 0.0084 0.0003 0.0042 0 0.0003 0.0018 0.0003 0
Mitochondria 0.0041 0.0001 0.0007 0.0002 0.0058 0.0006 0.0062 0 0.0048 0.0006 0.0243 0.0013 0.0012 0.0001 0.0006 0.0007 0.0084 0.0001
None 0.0017 0.0002 0.0014 0.0018 0.0221 0.0005 0.0022 0.0003 0.0007 0.0005 0.0053 0.0004 0.0052 0.0004 0.0005 0.0026 0.0018 0.0003
Nuclear Periphery 0.0113 0.0012 0.0036 0.006 0.0134 0.0045 0.0104 0.0014 0.0061 0.0048 0.0425 0.0037 0.0113 0.0012 0.0064 0.0065 0.0051 0.0022
Nucleolus 0.0002 0 0.0001 0 0.0005 0 0.0008 0 0.0001 0 0.0013 0 0.0003 0 0.0001 0.0029 0.0005 0
Nucleus 0.0352 0.0073 0.0319 0.0297 0.0406 0.0218 0.0552 0.0204 0.0414 0.0608 0.0855 0.0505 0.0423 0.0122 0.0181 0.0288 0.0427 0.0277
Peroxisomes 0.0003 0 0.0002 0 0.0007 0 0.0018 0 0.0004 0.0004 0.0012 0.0001 0.0051 0 0.0002 0.0005 0.0002 0
Punctate Nuclear 0.0019 0.0001 0.0007 0.0012 0.0019 0.0004 0.0032 0.0001 0.0012 0.0005 0.0095 0.0005 0.0112 0.0003 0.0009 0.016 0.0004 0.0003
Vacuole 0.0053 0.0004 0.0029 0.0005 0.0131 0.0008 0.0047 0.0003 0.0012 0.001 0.0093 0.0005 0.0009 0.0001 0.0002 0.0014 0.0102 0.0002
Vacuole Periphery 0.0017 0.0001 0.0005 0.0002 0.0048 0.0003 0.0014 0 0.0007 0.0002 0.0062 0.0002 0.0005 0 0.0002 0.0003 0.0041 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 135.2093 132.3802 140.4074 148.1906 141.3916 119.8514 130.9397 127.8989 148.7734 138.7618
Translational Efficiency 2.2565 2.2238 1.8871 2.1609 2.0824 2.5802 2.2201 1.9056 1.7548 1.8958

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Gamma-glutamyl phosphate reductase; catalyzes the second step in proline biosynthesis
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Pro2

Pro2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pro2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available