Standard name | |
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Human Ortholog | |
Description | Gamma-glutamyl phosphate reductase; catalyzes the second step in proline biosynthesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 4 | 1 | 1 | 2 | 4 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 3 | 3 | 10 | 10 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 4 | 8 | 3 | 8 | 9 | 20 | 25 | 40 | 32 | 34 | 24 | 2 | 4 | 2 | 5 | 12 | 8 | 0 | 0 | 2 | 0 | 0 | 0 |
Cytoplasm | 263 | 432 | 595 | 207 | 290 | 413 | 422 | 455 | 486 | 510 | 438 | 442 | 365 | 545 | 574 | 134 | 306 | 250 | 252 | 413 | 586 | 353 | 584 | 618 |
Endoplasmic Reticulum | 1 | 4 | 4 | 3 | 3 | 11 | 19 | 18 | 34 | 27 | 12 | 14 | 2 | 0 | 5 | 4 | 9 | 3 | 0 | 2 | 5 | 0 | 2 | 5 |
Endosome | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 3 | 1 | 5 | 0 | 1 | 1 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 2 | 2 | 0 | 1 |
Mitochondria | 2 | 2 | 2 | 3 | 6 | 54 | 75 | 57 | 72 | 175 | 174 | 155 | 0 | 0 | 0 | 2 | 2 | 1 | 2 | 1 | 6 | 1 | 1 | 0 |
Nucleus | 1 | 1 | 4 | 0 | 0 | 3 | 10 | 7 | 8 | 12 | 6 | 3 | 2 | 0 | 4 | 2 | 1 | 5 | 0 | 4 | 2 | 4 | 5 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 3 | 2 | 22 | 11 | 25 | 50 | 79 | 86 | 118 | 91 | 103 | 1 | 25 | 7 | 2 | 13 | 9 | 0 | 2 | 3 | 2 | 8 | 3 |
Unique Cell Count | 264 | 441 | 607 | 232 | 310 | 492 | 563 | 601 | 669 | 787 | 668 | 669 | 368 | 556 | 583 | 138 | 320 | 260 | 264 | 436 | 626 | 369 | 610 | 643 |
Labelled Cell Count | 269 | 449 | 617 | 238 | 319 | 523 | 600 | 645 | 731 | 888 | 774 | 756 | 372 | 574 | 592 | 150 | 347 | 276 | 264 | 436 | 626 | 369 | 610 | 643 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.4 | 14.9 | 14.0 | 13.3 | 12.5 | 10.9 | 9.9 | 10.0 | 9.7 | 8.8 | 8.3 | 8.5 | 17.3 | 16.9 | 15.0 | 18.9 | 17.1 | 18.1 | 18.4 | 18.2 | 18.4 |
Std Deviation (1e-4) | 2.4 | 2.8 | 2.8 | 2.5 | 2.3 | 2.2 | 2.0 | 2.0 | 1.9 | 1.8 | 2.0 | 2.0 | 3.4 | 3.2 | 3.6 | 5.7 | 4.4 | 5.5 | 3.2 | 3.6 | 3.5 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.16 | -0.36 | -0.5 | -0.49 | -0.53 | -0.67 | -0.75 | -0.73 | 0.31 | 0.26 | 0.1 | 0.43 | 0.28 | 0.36 | 0.39 | 0.37 | 0.39 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 1.4 | 0.7 | 2.5 | 3.0 | 4.4 | 3.0 | 3.8 | 2.6 | 0 | -1.0 | 0 | 0 | 2.4 | 0 |
Cytoplasm | -5.6 | -3.5 | -8.4 | -11.7 | -11.5 | -12.6 | -15.2 | -14.8 | -14.6 | 1.4 | 0 | 0.6 | -0.7 | -2.1 | -1.6 |
Endoplasmic Reticulum | 0 | 0 | 2.2 | 3.3 | 3.0 | 4.6 | 3.5 | 1.8 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 8.0 | 9.0 | 7.4 | 8.0 | 12.2 | 13.3 | 12.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 1.8 | 0.9 | 1.0 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 7.1 | 0 | 5.1 | 7.1 | 8.9 | 8.8 | 9.7 | 9.1 | 9.8 | 0 | 4.7 | 0 | 0 | 4.3 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 18.4898 | 23.0338 | 20.5319 | 18.9139 | 17.8319 | 20.5073 | 20.2566 | 27.2047 | 23.6405 | 23.0681 | 18.5266 | 23.2372 | 17.6667 | 23.4823 | 20.6031 | 18.476 | 19.0328 | 20.161 |
Actin | 0.0048 | 0.0003 | 0.0023 | 0.0009 | 0.001 | 0.0005 | 0.0146 | 0.0002 | 0.0012 | 0.0008 | 0.0119 | 0.0005 | 0.021 | 0.0005 | 0.0065 | 0.0007 | 0.0004 | 0.0001 |
Bud | 0.0004 | 0.0001 | 0.0005 | 0.0002 | 0.0019 | 0.0002 | 0.0009 | 0 | 0.0001 | 0.0002 | 0.0014 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0001 |
Bud Neck | 0.0004 | 0.0004 | 0.0008 | 0.0006 | 0.0005 | 0.0009 | 0.0015 | 0.0001 | 0.0004 | 0.0004 | 0.0013 | 0.0008 | 0.0004 | 0.0001 | 0.0004 | 0.0005 | 0.0006 | 0.0004 |
Bud Periphery | 0.0002 | 0 | 0.0005 | 0.0001 | 0.0013 | 0.0001 | 0.001 | 0 | 0.0001 | 0.0001 | 0.0018 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 |
Bud Site | 0.0005 | 0.0003 | 0.0007 | 0.0001 | 0.0062 | 0 | 0.0021 | 0 | 0.001 | 0.0001 | 0.0012 | 0 | 0.0007 | 0.0001 | 0.0002 | 0.0011 | 0.0005 | 0 |
Cell Periphery | 0.0001 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.9133 | 0.9886 | 0.9432 | 0.955 | 0.8271 | 0.9623 | 0.8503 | 0.9733 | 0.9236 | 0.912 | 0.7133 | 0.936 | 0.8678 | 0.9837 | 0.9612 | 0.9181 | 0.9068 | 0.967 |
Cytoplasmic Foci | 0.003 | 0.0004 | 0.0034 | 0.0009 | 0.0098 | 0.0018 | 0.0091 | 0.0003 | 0.0018 | 0.0071 | 0.0162 | 0.0009 | 0.013 | 0.0005 | 0.0016 | 0.0083 | 0.003 | 0.0006 |
Eisosomes | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0037 | 0.0005 | 0.0016 | 0.002 | 0.0077 | 0.0032 | 0.0061 | 0.0033 | 0.0047 | 0.0011 | 0.0126 | 0.0011 | 0.0039 | 0.0004 | 0.0011 | 0.0035 | 0.0034 | 0.0007 |
Endosome | 0.0108 | 0.0002 | 0.0022 | 0.0005 | 0.0309 | 0.002 | 0.0173 | 0.0001 | 0.0048 | 0.0067 | 0.0404 | 0.002 | 0.0079 | 0.0002 | 0.0009 | 0.0051 | 0.0102 | 0.0002 |
Golgi | 0.0008 | 0 | 0.0005 | 0 | 0.0021 | 0.0001 | 0.0046 | 0 | 0.0011 | 0.0024 | 0.0059 | 0.001 | 0.0026 | 0 | 0.0003 | 0.0008 | 0.0004 | 0 |
Lipid Particles | 0.0003 | 0 | 0.0021 | 0 | 0.0082 | 0 | 0.006 | 0 | 0.0044 | 0.0003 | 0.0084 | 0.0003 | 0.0042 | 0 | 0.0003 | 0.0018 | 0.0003 | 0 |
Mitochondria | 0.0041 | 0.0001 | 0.0007 | 0.0002 | 0.0058 | 0.0006 | 0.0062 | 0 | 0.0048 | 0.0006 | 0.0243 | 0.0013 | 0.0012 | 0.0001 | 0.0006 | 0.0007 | 0.0084 | 0.0001 |
None | 0.0017 | 0.0002 | 0.0014 | 0.0018 | 0.0221 | 0.0005 | 0.0022 | 0.0003 | 0.0007 | 0.0005 | 0.0053 | 0.0004 | 0.0052 | 0.0004 | 0.0005 | 0.0026 | 0.0018 | 0.0003 |
Nuclear Periphery | 0.0113 | 0.0012 | 0.0036 | 0.006 | 0.0134 | 0.0045 | 0.0104 | 0.0014 | 0.0061 | 0.0048 | 0.0425 | 0.0037 | 0.0113 | 0.0012 | 0.0064 | 0.0065 | 0.0051 | 0.0022 |
Nucleolus | 0.0002 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0013 | 0 | 0.0003 | 0 | 0.0001 | 0.0029 | 0.0005 | 0 |
Nucleus | 0.0352 | 0.0073 | 0.0319 | 0.0297 | 0.0406 | 0.0218 | 0.0552 | 0.0204 | 0.0414 | 0.0608 | 0.0855 | 0.0505 | 0.0423 | 0.0122 | 0.0181 | 0.0288 | 0.0427 | 0.0277 |
Peroxisomes | 0.0003 | 0 | 0.0002 | 0 | 0.0007 | 0 | 0.0018 | 0 | 0.0004 | 0.0004 | 0.0012 | 0.0001 | 0.0051 | 0 | 0.0002 | 0.0005 | 0.0002 | 0 |
Punctate Nuclear | 0.0019 | 0.0001 | 0.0007 | 0.0012 | 0.0019 | 0.0004 | 0.0032 | 0.0001 | 0.0012 | 0.0005 | 0.0095 | 0.0005 | 0.0112 | 0.0003 | 0.0009 | 0.016 | 0.0004 | 0.0003 |
Vacuole | 0.0053 | 0.0004 | 0.0029 | 0.0005 | 0.0131 | 0.0008 | 0.0047 | 0.0003 | 0.0012 | 0.001 | 0.0093 | 0.0005 | 0.0009 | 0.0001 | 0.0002 | 0.0014 | 0.0102 | 0.0002 |
Vacuole Periphery | 0.0017 | 0.0001 | 0.0005 | 0.0002 | 0.0048 | 0.0003 | 0.0014 | 0 | 0.0007 | 0.0002 | 0.0062 | 0.0002 | 0.0005 | 0 | 0.0002 | 0.0003 | 0.0041 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 135.2093 | 132.3802 | 140.4074 | 148.1906 | 141.3916 | 119.8514 | 130.9397 | 127.8989 | 148.7734 | 138.7618 |
Translational Efficiency | 2.2565 | 2.2238 | 1.8871 | 2.1609 | 2.0824 | 2.5802 | 2.2201 | 1.9056 | 1.7548 | 1.8958 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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