Standard name
Human Ortholog
Description RNA polymerase I largest subunit A190
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.09 0 0.06 0 0 0 0 0 – 0 0 0.11 0.07 0.08 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.5 0.7 0.35 0.35 0.3 0.28 0.21 0.13 0.2 – 0.15 0.16 0.46 0.39 0.46 0.08 0.07 0.05 0 0 0 0 0 0
Nucleus 0.06 0.09 0.07 0.1 0.06 0.07 0.06 0.05 0.05 – 0 0 0.07 0.1 0 0.13 0.05 0.08 0.05 0.05 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.96 0.84 0.94 0.79 0.87 0.9 0.9 0.89 0.92 – 0.9 0.9 0.84 0.82 0.88 0.91 0.87 0.86 0.87 0.85 0.83 0.87 0.81 0.72
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0.07 0.07 0.12 0.11 – 0.16 0.16 0 0 0 0 0 0 0 0 0 0 0 0.06
Vac/Vac Membrane 0 0 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 1 1 0 0 1
Bud 0 0 0 0 1 1 0 2 3 – 0 0 0 0 0 0 0 0 0 0 1 0 1 4
Bud Neck 1 0 0 1 0 4 2 11 2 – 2 4 0 0 0 0 0 0 1 1 3 0 0 2
Bud Site 0 0 1 0 1 2 9 8 18 – 7 15 0 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 0 25 5 19 14 6 8 16 4 – 4 6 10 14 18 3 5 4 0 2 3 0 2 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 2 0 0 0 0 0 0
Endosome 0 0 2 3 2 4 3 8 0 – 0 1 0 4 5 0 2 0 0 0 3 0 1 3
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 1 0 1 4
Mitochondria 66 186 136 112 112 128 124 95 89 – 55 64 41 79 104 9 8 5 1 0 5 0 4 8
Nucleus 8 23 29 34 24 31 35 33 23 – 11 12 6 21 5 14 6 8 6 14 16 3 7 3
Nuclear Periphery 0 1 0 0 0 1 0 1 0 – 1 0 1 0 0 0 0 0 0 2 1 0 0 1
Nucleolus 126 222 371 255 324 404 531 635 413 – 322 366 76 168 200 96 103 88 119 225 343 71 157 167
Peroxisomes 0 1 5 3 3 15 14 32 7 – 12 21 1 2 5 0 2 1 0 0 2 0 1 1
SpindlePole 4 0 5 9 9 32 40 82 49 – 56 65 0 4 9 3 3 2 5 3 17 1 4 14
Vac/Vac Membrane 0 3 4 21 4 2 3 1 3 – 0 2 0 2 1 0 3 1 0 6 8 2 9 13
Unique Cell Count 131 264 394 324 371 450 592 713 449 358 406 90 204 228 106 118 102 137 265 415 83 196 232
Labelled Cell Count 205 461 558 457 494 630 770 924 611 470 556 135 296 349 125 134 111 137 265 415 83 196 232


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 46.3 53.2 29.1 29.0 24.2 18.3 16.3 15.1 12.8 – 12.3 11.6 34.8 30.0 25.8 36.7 48.9 46.9 35.9 37.5 38.3
Std Deviation (1e-4) 16.5 32.0 10.2 11.0 10.9 6.5 5.3 5.4 4.7 – 4.5 4.3 20.4 15.3 12.7 17.6 25.1 21.7 15.0 15.1 17.8
Intensity Change (Log2) – – – -0.01 -0.26 -0.67 -0.84 -0.94 -1.18 – -1.24 -1.32 0.26 0.04 -0.17 0.33 0.75 0.69 0.3 0.37 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.7 – 0 3.5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 3.4 2.2 0.1 0.1 1.1 0 – 0 0.3 0 3.7 4.2 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 -1.3 -1.9 -4.7 -8.3 -4.8 – -6.0 -6.1 2.0 1.0 2.7 -5.2 -5.9 -5.9
Nucleus 1.5 -0.5 -0.3 -0.9 -1.9 -1.3 – -2.6 -2.8 -0.2 1.2 -2.7 1.9 -0.9 0.2
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus -6.2 -3.3 -2.3 -2.5 -2.8 -1.2 – -2.1 -2.1 -3.1 -4.6 -2.8 -1.3 -2.5 -2.7
Peroxisomes 0 0 2.0 1.2 2.9 0.4 – 1.9 3.1 0 0 0 0 0 0
SpindlePole 1.5 1.2 4.1 4.0 6.1 5.7 – 7.2 7.4 0 0 2.2 0 0 0
Vacuole 4.0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 28.7588 42.2405 38.0797 37.1282 29.5623 37.4897 34.7008 45.8436 40.7704 41.7061 38.9748 38.5807 38.0384 50.5911 35.6595 31.4843 27.7868 34.4908
Actin 0.041 0.0002 0.0028 0.012 0.0447 0.0026 0.0002 0.0002 0 0 0 0 0.0695 0 0 0 0.1237 0
Bud 0.0004 0 0.0001 0.0002 0.0007 0.0001 0.0001 0 0 0 0 0 0.0001 0 0 0 0.0006 0
Bud Neck 0.0012 0 0.0002 0.0008 0.0019 0.0001 0.0001 0.0003 0 0 0 0.0001 0.0001 0 0 0 0.0006 0
Bud Periphery 0.0005 0.0001 0.0001 0.0004 0.0014 0.0001 0.0001 0 0 0 0 0 0.0002 0 0 0 0.0011 0
Bud Site 0.0019 0.0001 0.0009 0.0016 0.0027 0.0003 0.0005 0.0001 0 0 0 0.0001 0.0002 0 0 0 0.003 0
Cell Periphery 0.0004 0.0001 0.0002 0.0003 0.0009 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0.0002 0.0001
Cytoplasm 0.0048 0 0.0001 0.0002 0.0012 0.0002 0 0 0 0 0 0 0.0001 0 0 0 0.0005 0
Cytoplasmic Foci 0.0294 0.0001 0.0028 0.0143 0.01 0.0075 0.0097 0.0022 0.0015 0.0001 0.0001 0.0002 0.0037 0 0.0008 0.0027 0.0455 0.0001
Eisosomes 0.0029 0.0002 0.0001 0.0003 0.0006 0.0001 0.0001 0 0 0 0 0 0.0002 0 0 0 0.0007 0
Endoplasmic Reticulum 0.003 0 0.0001 0.0003 0.0048 0.001 0 0 0 0 0 0 0.0001 0 0 0 0.0016 0
Endosome 0.0195 0 0.0025 0.0038 0.0155 0.0037 0.0001 0.0006 0 0 0 0 0.0009 0 0 0 0.0117 0
Golgi 0.0064 0 0.004 0.0032 0.006 0.0017 0.0004 0.0011 0 0 0 0 0.0033 0 0 0 0.0259 0
Lipid Particles 0.0141 0.0001 0.0039 0.0043 0.0221 0.0051 0.0045 0.0023 0.0009 0.0001 0.0003 0.0003 0.0082 0.0001 0.0029 0.0015 0.0485 0.0002
Mitochondria 0.0132 0.0106 0.0105 0.0076 0.1402 0.0046 0.0044 0.0035 0.0003 0.0003 0.0131 0.0003 0.0007 0.0005 0.001 0.0143 0.0095 0.0003
None 0.0134 0 0.0002 0.001 0.001 0.0002 0.0002 0 0 0 0 0 0.0006 0 0 0 0.0014 0
Nuclear Periphery 0.0297 0.0001 0.0008 0.0149 0.0054 0.0016 0.0002 0 0.0001 0 0 0 0.0003 0 0 0 0.0057 0
Nucleolus 0.7504 0.8729 0.9168 0.8604 0.676 0.917 0.8879 0.9034 0.9176 0.9036 0.9318 0.9401 0.8416 0.9683 0.9532 0.9634 0.6454 0.95
Nucleus 0.039 0.1139 0.0512 0.0691 0.0382 0.0504 0.0488 0.0819 0.077 0.0957 0.0536 0.0566 0.0471 0.0309 0.0414 0.0167 0.021 0.0491
Peroxisomes 0.007 0 0.0013 0.0012 0.0075 0.0014 0.0019 0.0006 0 0 0.0003 0.0001 0.0216 0 0.0001 0.0005 0.04 0
Punctate Nuclear 0.0185 0.0012 0.0003 0.0012 0.0007 0.0013 0.04 0.003 0.0023 0 0.0001 0.0019 0.0013 0 0.0001 0.0001 0.0122 0.0001
Vacuole 0.0011 0.0001 0.0003 0.0008 0.0106 0.0004 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0.0002 0.0003 0
Vacuole Periphery 0.0022 0.0002 0.0009 0.002 0.0079 0.0004 0.0004 0.0003 0.0001 0 0.0005 0.0001 0.0001 0 0.0003 0.0004 0.0009 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 236.6963 209.716 198.6991 184.4197 179.0055 147.2599 161.6783 146.0125 108.5402 176.3633
Translational Efficiency 0.6318 0.743 0.6467 0.5464 0.5779 0.7199 0.6682 0.4771 0.5488 0.5153

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description RNA polymerase I largest subunit A190
Localization
Cell Percentages nucleolus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Rpa190

Rpa190


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpa190-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available