Standard name | |
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Human Ortholog | |
Description | Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 4 |
Bud | 0 | 0 | 7 | 0 | 0 | 0 | 4 | 4 | 2 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 2 | 4 | 0 | 0 | 6 |
Bud Neck | 0 | 0 | 7 | 0 | 4 | 6 | 1 | 8 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 3 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 89 | 132 | 199 | 185 | 281 | 325 | 339 | 400 | 150 | 129 | 172 | 228 | 86 | 283 | 313 | 234 | 106 | 164 | 66 | 107 | 120 | 46 | 142 | 117 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 17 | 3 | 18 | 1 | 0 | 0 | 0 | 0 | 1 |
Endosome | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 9 | 4 | 3 | 2 | 2 | 0 | 0 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 1 | 3 | 1 | 1 | 1 | 0 | 0 | 0 |
Mitochondria | 1 | 28 | 56 | 42 | 12 | 43 | 78 | 92 | 50 | 36 | 94 | 104 | 9 | 1 | 11 | 13 | 2 | 7 | 11 | 10 | 29 | 3 | 11 | 7 |
Nucleus | 30 | 20 | 5 | 2 | 1 | 0 | 2 | 6 | 0 | 1 | 3 | 0 | 3 | 1 | 0 | 19 | 8 | 14 | 36 | 43 | 25 | 9 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 1 | 3 | 3 | 1 | 4 | 6 | 1 | 3 | 4 | 0 | 0 | 0 | 1 | 1 | 1 | 4 | 10 | 11 | 0 | 2 | 3 |
Vac/Vac Membrane | 24 | 48 | 5 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 10 | 9 | 3 | 67 | 45 | 49 | 31 | 49 | 46 | 11 | 11 | 7 |
Unique Cell Count | 136 | 203 | 251 | 207 | 289 | 341 | 366 | 443 | 169 | 144 | 227 | 285 | 99 | 291 | 320 | 324 | 152 | 231 | 170 | 237 | 267 | 79 | 179 | 162 |
Labelled Cell Count | 146 | 231 | 281 | 230 | 302 | 379 | 430 | 522 | 210 | 169 | 273 | 343 | 108 | 297 | 329 | 377 | 177 | 261 | 170 | 237 | 267 | 79 | 179 | 162 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 3.7 | 3.0 | 2.7 | 3.1 | 2.8 | 2.7 | 2.6 | 2.4 | 2.2 | 2.4 | 2.3 | 4.0 | 4.2 | 3.6 | 5.5 | 6.2 | 5.9 | 3.7 | 4.3 | 5.4 |
Std Deviation (1e-4) | 1.0 | 0.8 | 0.8 | 0.7 | 1.3 | 1.3 | 1.2 | 1.1 | 1.7 | 0.9 | 1.2 | 1.4 | 1.4 | 1.3 | 1.1 | 1.5 | 2.4 | 2.1 | 0.6 | 1.2 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.15 | 0.05 | -0.1 | -0.16 | -0.2 | -0.32 | -0.42 | -0.3 | -0.4 | 0.42 | 0.49 | 0.26 | 0.89 | 1.06 | 0.99 | 0.3 | 0.51 | 0.85 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 6.7 | 8.1 | 6.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.233 | 0.2572 | 0.2723 | 0.2398 | -0.1186 | 0.186 | 2.4678 | 2.1688 | 2.2067 | 1.8513 | 1.8588 | 2.2397 | 2.0064 | 2.577 | 2.6599 | 2.6242 | 2.7665 | 2.6734 |
Actin | 0.029 | 0.0079 | 0.0126 | 0.0216 | 0.0021 | 0.002 | 0.031 | 0.0002 | 0.0203 | 0.0001 | 0.0029 | 0.0008 | 0.0013 | 0.0003 | 0.0132 | 0.0245 | 0.007 | 0.0015 |
Bud | 0.001 | 0.0014 | 0.0003 | 0.0005 | 0.0007 | 0.003 | 0.0008 | 0.0001 | 0.0015 | 0.0001 | 0.0014 | 0.0003 | 0.0003 | 0.0002 | 0.0005 | 0.0017 | 0.0003 | 0.0003 |
Bud Neck | 0.0148 | 0.0004 | 0.0014 | 0.0008 | 0.0028 | 0.0063 | 0.0013 | 0.0003 | 0.001 | 0.0003 | 0.0006 | 0.0076 | 0.0004 | 0.0004 | 0.0008 | 0.0018 | 0.0009 | 0.0043 |
Bud Periphery | 0.0019 | 0.0042 | 0.0006 | 0.0029 | 0.0008 | 0.0031 | 0.0015 | 0.0001 | 0.0048 | 0 | 0.003 | 0.0003 | 0.0004 | 0.0002 | 0.0009 | 0.0051 | 0.0002 | 0.0004 |
Bud Site | 0.009 | 0.0025 | 0.003 | 0.0006 | 0.0035 | 0.0069 | 0.0054 | 0.0016 | 0.0067 | 0.0001 | 0.0016 | 0.0004 | 0.0007 | 0.0058 | 0.0021 | 0.0057 | 0.0003 | 0.0004 |
Cell Periphery | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0012 | 0 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0008 | 0 | 0.0001 |
Cytoplasm | 0.1807 | 0.2244 | 0.1859 | 0.2223 | 0.313 | 0.2225 | 0.094 | 0.154 | 0.1428 | 0.0999 | 0.1341 | 0.1732 | 0.2023 | 0.2022 | 0.1695 | 0.1032 | 0.164 | 0.1964 |
Cytoplasmic Foci | 0.026 | 0.0079 | 0.0099 | 0.0064 | 0.0343 | 0.0132 | 0.0196 | 0.0056 | 0.0132 | 0.0053 | 0.0266 | 0.008 | 0.0097 | 0.0072 | 0.008 | 0.0019 | 0.0129 | 0.014 |
Eisosomes | 0.0006 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0094 | 0.0049 | 0.0036 | 0.0022 | 0.0059 | 0.0043 | 0.0088 | 0.0022 | 0.0033 | 0.0007 | 0.0028 | 0.0015 | 0.0059 | 0.0049 | 0.0046 | 0.0014 | 0.0026 | 0.0017 |
Endosome | 0.0215 | 0.0038 | 0.0058 | 0.0041 | 0.0106 | 0.007 | 0.022 | 0.0027 | 0.0152 | 0.0007 | 0.0089 | 0.0022 | 0.0074 | 0.0044 | 0.0063 | 0.005 | 0.0122 | 0.0065 |
Golgi | 0.0069 | 0.0014 | 0.003 | 0.0016 | 0.0018 | 0.0007 | 0.0051 | 0.0001 | 0.0088 | 0 | 0.001 | 0.0002 | 0.0009 | 0.0004 | 0.0015 | 0.0043 | 0.011 | 0.0004 |
Lipid Particles | 0.0176 | 0.0025 | 0.0019 | 0.0036 | 0.0066 | 0.0015 | 0.0136 | 0.0004 | 0.0048 | 0.0001 | 0.0098 | 0.0003 | 0.0046 | 0.0003 | 0.0034 | 0.0012 | 0.0035 | 0.0008 |
Mitochondria | 0.0056 | 0.0016 | 0.0149 | 0.0496 | 0.0036 | 0.0091 | 0.0044 | 0.0006 | 0.0072 | 0.0004 | 0.0043 | 0.0006 | 0.001 | 0.0011 | 0.0037 | 0.0043 | 0.0039 | 0.0008 |
None | 0.4906 | 0.5156 | 0.5349 | 0.3523 | 0.3046 | 0.3673 | 0.6967 | 0.7005 | 0.6363 | 0.664 | 0.5692 | 0.591 | 0.6927 | 0.5238 | 0.5677 | 0.4803 | 0.5813 | 0.5815 |
Nuclear Periphery | 0.0302 | 0.0067 | 0.0131 | 0.0081 | 0.0126 | 0.0077 | 0.0175 | 0.0037 | 0.0066 | 0.0017 | 0.0184 | 0.0073 | 0.0083 | 0.0064 | 0.0151 | 0.0035 | 0.0057 | 0.0032 |
Nucleolus | 0.0057 | 0.0022 | 0.0028 | 0.0054 | 0.0033 | 0.004 | 0.0032 | 0.0027 | 0.0018 | 0.0008 | 0.0035 | 0.0024 | 0.0008 | 0.0018 | 0.0018 | 0.0012 | 0.0016 | 0.0026 |
Nucleus | 0.1137 | 0.1988 | 0.1752 | 0.2469 | 0.2636 | 0.3121 | 0.0442 | 0.113 | 0.0966 | 0.2206 | 0.1723 | 0.1659 | 0.0479 | 0.2303 | 0.1708 | 0.3468 | 0.1508 | 0.1644 |
Peroxisomes | 0.0115 | 0.0003 | 0.0021 | 0.0515 | 0.0069 | 0.0009 | 0.0086 | 0.0002 | 0.0066 | 0.0002 | 0.0011 | 0.0009 | 0.0032 | 0.0002 | 0.0031 | 0.0005 | 0.0062 | 0.0008 |
Punctate Nuclear | 0.0165 | 0.0097 | 0.026 | 0.0163 | 0.0185 | 0.0255 | 0.0163 | 0.0097 | 0.0176 | 0.0044 | 0.0338 | 0.0353 | 0.0068 | 0.0072 | 0.0242 | 0.0045 | 0.0319 | 0.0181 |
Vacuole | 0.0057 | 0.0028 | 0.0022 | 0.0017 | 0.004 | 0.0025 | 0.0036 | 0.002 | 0.0023 | 0.0004 | 0.0028 | 0.0011 | 0.0045 | 0.0025 | 0.0017 | 0.0016 | 0.0031 | 0.0016 |
Vacuole Periphery | 0.0015 | 0.0003 | 0.0005 | 0.001 | 0.0006 | 0.0004 | 0.0015 | 0.0002 | 0.0008 | 0.0001 | 0.0018 | 0.0007 | 0.0008 | 0.0003 | 0.0006 | 0.0005 | 0.0004 | 0.0002 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.8693 | 13.005 | 15.094 | 17.5272 | 10.6401 | 11.5573 | 17.5324 | 16.4982 | 13.6603 | 13.1088 |
Translational Efficiency | 0.3339 | 0.5339 | 0.4642 | 0.3511 | 0.3145 | 0.2974 | 0.3659 | 0.3247 | 0.4055 | 0.2848 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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