| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 3 | 3 | 1 |
| Bud | 0 | 2 | 3 | 0 | 2 | 5 | 5 | 14 | 5 | 5 | 11 | 7 | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 3 | 3 |
| Bud Neck | 0 | 0 | 6 | 2 | 2 | 7 | 8 | 9 | 1 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 3 | 2 | 2 | 4 | 3 | 5 | 1 | 1 | 2 | 4 | 3 | 3 | 1 | 0 | 1 | 9 | 6 | 10 | 0 | 0 | 6 | 0 | 0 | 0 |
| Cytoplasm | 177 | 259 | 441 | 384 | 437 | 536 | 602 | 594 | 401 | 375 | 308 | 314 | 210 | 305 | 320 | 234 | 161 | 195 | 171 | 239 | 335 | 184 | 228 | 247 |
| Endoplasmic Reticulum | 2 | 0 | 1 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 5 | 0 | 11 | 7 | 12 | 0 | 1 | 1 | 1 | 1 | 4 |
| Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 0 | 0 | 1 | 2 | 0 | 2 | 1 |
| Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
| Mitochondria | 0 | 10 | 3 | 14 | 20 | 72 | 105 | 149 | 138 | 126 | 183 | 146 | 1 | 1 | 8 | 7 | 1 | 4 | 3 | 13 | 24 | 3 | 6 | 9 |
| Nucleus | 1 | 1 | 0 | 1 | 1 | 3 | 2 | 2 | 3 | 3 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
| SpindlePole | 0 | 0 | 1 | 0 | 0 | 2 | 6 | 5 | 3 | 6 | 3 | 6 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 3 | 1 | 0 | 0 |
| Vac/Vac Membrane | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 2 | 1 | 1 | 2 | 0 | 1 | 0 | 5 | 6 | 2 | 0 | 3 | 2 | 1 | 1 | 5 |
| Unique Cell Count | 182 | 265 | 446 | 390 | 448 | 557 | 640 | 666 | 442 | 434 | 402 | 381 | 211 | 311 | 323 | 248 | 173 | 202 | 183 | 265 | 399 | 204 | 255 | 282 |
| Labelled Cell Count | 187 | 275 | 458 | 410 | 466 | 632 | 733 | 781 | 555 | 521 | 517 | 484 | 214 | 314 | 335 | 274 | 188 | 227 | 183 | 265 | 399 | 204 | 255 | 282 |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 4.8 | 3.6 | 3.2 | 3.1 | 3.4 | 3.0 | 2.9 | 3.1 | 2.6 | 2.7 | 2.6 | 2.5 | 4.2 | 4.4 | 4.0 | 6.1 | 7.1 | 6.6 | 4.6 | 4.6 | 5.1 |
| Std Deviation (1e-4) | 0.5 | 0.5 | 0.7 | 0.9 | 1.3 | 1.1 | 1.2 | 1.7 | 1.1 | 2.3 | 1.0 | 1.2 | 1.3 | 1.2 | 1.1 | 1.3 | 2.5 | 2.2 | 1.5 | 1.6 | 1.6 |
| Intensity Change (Log2) | – | – | – | -0.05 | 0.11 | -0.07 | -0.12 | -0.04 | -0.32 | -0.22 | -0.3 | -0.35 | 0.39 | 0.46 | 0.34 | 0.94 | 1.15 | 1.05 | 0.53 | 0.53 | 0.68 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Actin | 0.039 | 0.0004 | 0.0134 | 0.0495 | 0.0438 | 0.0117 | – | – | – | – | – | – | 0.0109 | 0.0002 | 0.0042 | 0.0027 | 0.01 | 0.0007 |
| Bud | 0.0017 | 0.0008 | 0.0009 | 0.0081 | 0.0006 | 0.0002 | – | – | – | – | – | – | 0.0052 | 0.0001 | 0.008 | 0.0001 | 0.0143 | 0.0017 |
| Bud Neck | 0.0027 | 0.0003 | 0.0032 | 0.001 | 0.0041 | 0.0012 | – | – | – | – | – | – | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.001 |
| Bud Periphery | 0.005 | 0.0007 | 0.0012 | 0.0089 | 0.0018 | 0.0003 | – | – | – | – | – | – | 0.0065 | 0.0001 | 0.0135 | 0.0001 | 0.0157 | 0.0007 |
| Bud Site | 0.0079 | 0.0124 | 0.0109 | 0.0045 | 0.0012 | 0.0012 | – | – | – | – | – | – | 0.0034 | 0.0012 | 0.0103 | 0.0001 | 0.0008 | 0.0001 |
| Cell Periphery | 0.001 | 0.0006 | 0.0006 | 0.0006 | 0.0004 | 0.0001 | – | – | – | – | – | – | 0.0004 | 0.0001 | 0.0009 | 0.0001 | 0.0003 | 0.0001 |
| Cytoplasm | 0.1771 | 0.3278 | 0.2384 | 0.2671 | 0.2663 | 0.3566 | – | – | – | – | – | – | 0.0969 | 0.2531 | 0.1755 | 0.1711 | 0.1759 | 0.2112 |
| Cytoplasmic Foci | 0.0128 | 0.0073 | 0.0151 | 0.0104 | 0.0107 | 0.0194 | – | – | – | – | – | – | 0.0077 | 0.0045 | 0.0057 | 0.0078 | 0.02 | 0.014 |
| Eisosomes | 0.0012 | 0.0001 | 0.0004 | 0.0027 | 0.0013 | 0.0002 | – | – | – | – | – | – | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0 |
| Endoplasmic Reticulum | 0.005 | 0.0025 | 0.003 | 0.0021 | 0.0038 | 0.0017 | – | – | – | – | – | – | 0.001 | 0.0012 | 0.0015 | 0.0003 | 0.0008 | 0.0005 |
| Endosome | 0.0101 | 0.0025 | 0.009 | 0.0061 | 0.0059 | 0.0031 | – | – | – | – | – | – | 0.0013 | 0.0014 | 0.0025 | 0.0007 | 0.0011 | 0.0013 |
| Golgi | 0.0039 | 0.0003 | 0.0039 | 0.007 | 0.0072 | 0.0016 | – | – | – | – | – | – | 0.0007 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0002 |
| Lipid Particles | 0.004 | 0.0017 | 0.0077 | 0.0027 | 0.0138 | 0.0026 | – | – | – | – | – | – | 0.0038 | 0.0002 | 0.001 | 0.0003 | 0.0005 | 0.0002 |
| Mitochondria | 0.0031 | 0.0005 | 0.0102 | 0.0972 | 0.0348 | 0.0017 | – | – | – | – | – | – | 0.0009 | 0.0002 | 0.0014 | 0.0001 | 0.0016 | 0.0008 |
| None | 0.7013 | 0.6364 | 0.6593 | 0.5134 | 0.5908 | 0.5887 | – | – | – | – | – | – | 0.8484 | 0.7337 | 0.7604 | 0.8144 | 0.738 | 0.7603 |
| Nuclear Periphery | 0.0037 | 0.0005 | 0.0039 | 0.0012 | 0.0013 | 0.0005 | – | – | – | – | – | – | 0.0004 | 0.0005 | 0.0018 | 0.0001 | 0.0012 | 0.0003 |
| Nucleolus | 0.0005 | 0.0002 | 0.0009 | 0.0007 | 0.0004 | 0.0003 | – | – | – | – | – | – | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0013 | 0.0003 |
| Nucleus | 0.0025 | 0.0019 | 0.002 | 0.0032 | 0.0011 | 0.0008 | – | – | – | – | – | – | 0.0008 | 0.0013 | 0.0024 | 0.0003 | 0.0035 | 0.0014 |
| Peroxisomes | 0.0024 | 0.0002 | 0.0101 | 0.0041 | 0.0071 | 0.0038 | – | – | – | – | – | – | 0.0089 | 0.0001 | 0.0032 | 0.0005 | 0.0073 | 0.0015 |
| Punctate Nuclear | 0.0088 | 0.0011 | 0.0036 | 0.0053 | 0.0018 | 0.0038 | – | – | – | – | – | – | 0.0011 | 0.0008 | 0.0041 | 0.0003 | 0.0055 | 0.0029 |
| Vacuole | 0.0046 | 0.0017 | 0.0017 | 0.0025 | 0.0011 | 0.0004 | – | – | – | – | – | – | 0.0008 | 0.0007 | 0.0014 | 0.0003 | 0.0011 | 0.0006 |
| Vacuole Periphery | 0.0016 | 0.0001 | 0.0007 | 0.0014 | 0.0004 | 0.0001 | – | – | – | – | – | – | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0004 | 0.0002 |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 3.986 | 2.5915 | 2.1634 | 4.981 | 3.7586 | 2.8973 | 4.9948 | 4.4212 | 5.5388 | 4.7585 |
| Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | – |
| Protein Localization | ✘ |
| Gene Expression | ✘ |
| Translational Efficiency | – |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl |
| Localization | |
| Cell Percentages | cytoplasm (22%) |
| Cell Cycle Regulation | No |
| Subcompartmental Group | cytoplasm-9 |
Cin1
Cin1
Nearest neighbours ( genes)
correlation threshold
0.80
Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Cin1-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
