Standard name
Human Ortholog
Description eIF3b subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes; eIF3 is also involved in programmed stop codon readthrough
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0.08 0.08 0.09 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0.05 – 0.08 0.08 0.08 0.14 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.94 0.94 0.85 0.81 – 0.71 0.62 0.66 0.62 0.95 0.96 0.98 0.98 0.98 0.94 0.96 0.95 0.93 0.83 0.83 0.78
Endoplasmic Reticulum 0 0 0 0 0.05 0.12 0.15 – 0.17 0.19 0.18 0.18 0 0 0 0 0 0 0 0 0 0.11 0.12 0.15
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.06 0.1 0.11 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 2 9 – 18 17 18 18 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 1 0 1 0 1 2 6
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 8 10 10 9 16 – 18 17 16 30 3 2 2 4 3 8 0 0 0 1 0 0
Cytoplasm 113 282 265 200 211 171 245 – 168 136 135 138 274 378 400 246 223 147 109 276 268 176 294 297
Endoplasmic Reticulum 0 2 7 6 11 24 46 – 40 41 37 40 12 17 14 5 8 5 0 2 5 24 41 58
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Golgi 0 0 0 0 0 2 6 – 4 9 5 19 0 0 0 0 0 1 0 1 0 1 0 1
Mitochondria 0 0 0 1 0 2 3 – 14 21 23 22 1 0 0 2 2 1 0 1 1 1 2 3
Nucleus 2 0 0 0 0 0 1 – 0 2 1 2 0 0 0 0 0 0 0 3 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 3 0 2 0 0 – 3 6 3 2 1 0 0 0 1 2 1 4 6 4 5 7
Unique Cell Count 114 284 273 213 224 201 303 235 219 205 222 288 393 410 250 227 156 115 293 288 213 354 380
Labelled Cell Count 115 287 284 217 234 210 326 266 249 239 271 291 398 416 257 237 166 115 293 288 213 354 380


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 64.7 74.0 50.0 48.9 41.7 34.5 31.4 – 27.2 25.6 24.9 24.8 60.8 56.3 53.2 65.4 65.9 51.2 55.5 56.2 57.5
Std Deviation (1e-4) 15.5 14.8 12.9 11.3 7.9 7.4 6.9 – 6.5 6.3 5.3 5.3 12.4 11.1 11.1 19.5 20.3 19.1 11.6 12.5 13.7
Intensity Change (Log2) – – – -0.03 -0.26 -0.53 -0.67 – -0.88 -0.97 -1.01 -1.01 0.28 0.17 0.09 0.39 0.4 0.03 0.15 0.17 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 4.3 4.3 4.7 4.5 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.0 0.9 0.9 1.4 – 2.4 2.4 2.4 4.4 -1.6 0 0 -1.0 0 1.2
Cytoplasm -1.7 -1.6 -4.8 -6.1 – -8.1 -9.9 -9.1 -9.9 -1.2 -0.6 0.4 1.0 0.8 -1.4
Endoplasmic Reticulum 0.2 1.4 4.1 5.2 – 5.6 6.0 5.8 5.8 1.0 1.2 0.6 -0.4 0.6 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 4.9 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 4.1 5.2 5.7 5.3 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 41.9178 63.6185 55.9795 50.6025 38.3457 56.654 50.0956 74.1437 69.0416 67.3302 59.1364 62.314 47.5397 72.567 67.1211 62.3479 42.0469 65.8748
Actin 0.0043 0.0002 0.0014 0.0011 0.0015 0.0011 0.0041 0.0002 0.0078 0.0004 0.0019 0.0021 0.0294 0.0021 0.014 0.0005 0.0215 0.0044
Bud 0.0014 0.0003 0.0015 0.0019 0.0017 0.0005 0.0018 0.0002 0.0021 0.0022 0.0019 0.0017 0.0014 0.0005 0.0007 0.0012 0.0044 0.0018
Bud Neck 0.0005 0.0004 0.0006 0.0006 0.0009 0.0014 0.0009 0.0002 0.0006 0.0006 0.0004 0.0014 0.0078 0.0003 0.002 0.0007 0.0124 0.0057
Bud Periphery 0.0017 0.0003 0.0015 0.0018 0.0037 0.0005 0.0026 0.0001 0.0024 0.0017 0.0016 0.002 0.0022 0.0005 0.0009 0.0003 0.0097 0.002
Bud Site 0.002 0.0003 0.0103 0.0065 0.0004 0.0001 0.0023 0.0002 0.0009 0.0004 0.0006 0.0002 0.0101 0.0049 0.0028 0.0003 0.002 0.0028
Cell Periphery 0.0049 0.0061 0.011 0.0124 0.0027 0.0005 0.0053 0.003 0.0058 0.0018 0.0032 0.0009 0.0082 0.0067 0.0029 0.0012 0.0034 0.0066
Cytoplasm 0.6208 0.9055 0.751 0.6799 0.5976 0.7964 0.7075 0.9545 0.865 0.8156 0.7848 0.8573 0.676 0.9522 0.8992 0.8132 0.602 0.8505
Cytoplasmic Foci 0.015 0.0009 0.0074 0.0223 0.0236 0.0056 0.0209 0.001 0.0078 0.0146 0.0216 0.0128 0.0259 0.0016 0.0028 0.0064 0.0181 0.0087
Eisosomes 0.0002 0 0.0001 0.0001 0.0003 0 0.0002 0 0.0001 0.0001 0.0008 0 0.0007 0.0001 0.0002 0.0005 0.0002 0
Endoplasmic Reticulum 0.0667 0.0469 0.0575 0.0104 0.0305 0.0755 0.0229 0.0114 0.0155 0.0081 0.0118 0.0236 0.0553 0.0053 0.0064 0.0037 0.0159 0.0326
Endosome 0.0196 0.0021 0.0131 0.0265 0.0398 0.0168 0.0254 0.0013 0.0071 0.0076 0.0256 0.0198 0.0331 0.0022 0.0106 0.0148 0.035 0.0311
Golgi 0.002 0.0003 0.005 0.0043 0.0054 0.009 0.0032 0.0002 0.0021 0.0004 0.002 0.0031 0.0068 0.0012 0.0036 0.0007 0.0058 0.0071
Lipid Particles 0.0093 0.0006 0.0043 0.003 0.0157 0.0168 0.0122 0.0008 0.0015 0.0023 0.015 0.0017 0.0125 0.0008 0.0012 0.0029 0.0024 0.0013
Mitochondria 0.0159 0.0011 0.0158 0.0356 0.0606 0.0446 0.0219 0.0001 0.0127 0.003 0.0316 0.007 0.0094 0.0007 0.0134 0.0157 0.0172 0.0084
None 0.0054 0.0002 0.0043 0.0029 0.0049 0.0003 0.0097 0.0002 0.0012 0.0092 0.0367 0.0051 0.0091 0.0006 0.0004 0.0057 0.0314 0.0005
Nuclear Periphery 0.0343 0.004 0.0115 0.0178 0.0131 0.0065 0.0241 0.0017 0.0044 0.0043 0.0063 0.008 0.0267 0.0027 0.003 0.0059 0.0579 0.0064
Nucleolus 0.015 0.0001 0.002 0.0005 0.0021 0.0001 0.0046 0.0001 0.0006 0.0011 0.0035 0.0009 0.0023 0.0001 0.0003 0.0103 0.0036 0.0002
Nucleus 0.0289 0.0016 0.0063 0.0038 0.0054 0.0019 0.0347 0.0011 0.0022 0.0024 0.0038 0.0042 0.009 0.0018 0.002 0.0053 0.0826 0.0027
Peroxisomes 0.0008 0 0.0005 0.0005 0.0015 0.0004 0.0069 0 0.0005 0.0005 0.0016 0.0001 0.0027 0.0001 0.0011 0.0008 0.0014 0.0008
Punctate Nuclear 0.011 0 0.004 0.0002 0.0008 0.0001 0.0072 0 0.0001 0.0004 0.0011 0.0014 0.0113 0.0006 0.0004 0.001 0.0313 0.0007
Vacuole 0.1065 0.0264 0.0784 0.1369 0.1282 0.0169 0.0689 0.0228 0.0518 0.1051 0.0307 0.0323 0.0463 0.0124 0.0288 0.0858 0.0291 0.0192
Vacuole Periphery 0.0338 0.0027 0.0124 0.0312 0.0598 0.0048 0.0129 0.0011 0.0077 0.0182 0.0133 0.0143 0.0137 0.0025 0.0033 0.023 0.0125 0.0068

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 185.345 194.0906 180.4287 146.4261 177.2661 178.9874 155.5599 130.3962 129.9995 160.0677
Translational Efficiency 1.8497 1.9241 1.5536 1.9197 1.6717 1.5706 1.6989 1.6191 1.5487 1.6044

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description eIF3b subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes; eIF3 is also involved in programmed stop codon readthrough
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Prt1

Prt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available