Standard name | |
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Human Ortholog | |
Description | Mitochondrial aldehyde dehydrogenase; required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed; can substitute for cytosolic NADP-dependent aldehyde dehydrogenase when directed to the cytosol; human homolog ALDH2 can complement yeast ald4 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 3 | 2 | 1 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 8 | 7 | 6 | 3 | 2 | 0 | 13 | 19 | 27 | 13 | 13 | 19 |
Bud | 5 | 0 | 2 | 5 | 1 | 0 | 0 | 3 | 1 | 1 | 3 | 4 | 7 | 5 | 3 | 0 | 0 | 0 | 2 | 5 | 0 | 1 | 2 | 4 |
Bud Neck | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 2 | 0 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 9 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 4 | 1 | 1 | 2 | 6 | 5 | 7 | 3 | 2 | 1 | 0 | 5 | 2 | 8 | 11 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 7 | 5 | 2 | 0 | 2 | 0 | 0 | 0 | 1 |
Endosome | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 2 | 2 | 0 | 5 | 6 | 1 | 2 | 5 | 7 |
Golgi | 1 | 8 | 4 | 4 | 5 | 1 | 1 | 0 | 2 | 0 | 5 | 4 | 13 | 15 | 18 | 34 | 30 | 6 | 14 | 32 | 12 | 15 | 20 | 19 |
Mitochondria | 259 | 252 | 153 | 143 | 110 | 184 | 266 | 282 | 209 | 292 | 212 | 251 | 178 | 221 | 186 | 49 | 21 | 3 | 222 | 278 | 132 | 86 | 131 | 119 |
Nucleus | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 4 | 1 | 0 | 1 | 2 | 0 | 2 | 1 | 0 | 1 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 0 |
Nucleolus | 27 | 31 | 14 | 15 | 11 | 38 | 61 | 73 | 57 | 66 | 43 | 60 | 8 | 14 | 16 | 84 | 29 | 16 | 2 | 6 | 1 | 3 | 8 | 2 |
Peroxisomes | 12 | 12 | 14 | 6 | 3 | 2 | 1 | 3 | 0 | 0 | 1 | 0 | 11 | 23 | 29 | 14 | 4 | 2 | 22 | 17 | 29 | 19 | 23 | 18 |
SpindlePole | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 7 | 1 | 0 | 4 | 4 | 1 | 1 | 4 | 5 |
Vac/Vac Membrane | 13 | 34 | 2 | 0 | 5 | 11 | 16 | 14 | 6 | 4 | 8 | 7 | 11 | 9 | 11 | 17 | 15 | 5 | 3 | 11 | 0 | 2 | 5 | 5 |
Unique Cell Count | 282 | 312 | 173 | 152 | 123 | 199 | 287 | 315 | 220 | 298 | 225 | 263 | 218 | 262 | 237 | 195 | 98 | 36 | 299 | 392 | 212 | 152 | 225 | 209 |
Labelled Cell Count | 330 | 358 | 198 | 180 | 140 | 247 | 353 | 385 | 279 | 370 | 275 | 330 | 244 | 304 | 279 | 235 | 118 | 39 | 299 | 392 | 212 | 152 | 225 | 209 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 19.6 | 25.3 | 31.2 | 31.7 | 37.9 | 41.7 | 43.2 | 46.1 | 50.8 | 50.2 | 50.8 | 51.0 | 30.4 | 31.1 | 30.7 | 56.8 | 62.5 | 57.4 | 17.0 | 20.6 | 20.0 |
Std Deviation (1e-4) | 8.2 | 12.7 | 14.2 | 15.4 | 15.3 | 18.0 | 18.4 | 22.5 | 22.4 | 24.2 | 23.4 | 24.1 | 11.3 | 11.4 | 13.1 | 29.9 | 29.3 | 32.6 | 6.8 | 11.3 | 7.6 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.28 | 0.42 | 0.47 | 0.56 | 0.7 | 0.68 | 0.7 | 0.71 | -0.04 | -0.01 | -0.02 | 0.86 | 1.0 | 0.88 | -0.88 | -0.6 | -0.65 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 1.7 | 2.3 | 4.8 | 6.8 | 0 |
Mitochondria | 1.8 | 0.3 | 1.3 | 1.5 | 0.4 | 2.4 | 4.4 | 2.1 | 2.7 | -1.8 | -1.2 | -2.6 | -12.2 | -11.1 | -10.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.6 | 0.3 | 3.1 | 3.7 | 4.2 | 4.6 | 3.9 | 3.1 | 4.0 | -1.9 | -1.1 | -0.5 | 7.6 | 4.7 | 5.7 |
Peroxisomes | -1.6 | -2.1 | -3.4 | -4.5 | -4.1 | -4.3 | -5.0 | -4.0 | -4.7 | -1.2 | 0.3 | 1.4 | -0.3 | -1.3 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 2.3 | 2.4 | 2.0 | 0 | 0 | 0 | 0 | 2.1 | 0 | 2.0 | 3.3 | 4.6 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0011 | 0.0124 | 0.0069 | 0.0034 | 0.0001 | 0.0004 | 0.0029 | 0.0008 | 0.0038 | 0.003 | 0.007 | 0 | – | – | – | – | – | – |
Bud | 0.0001 | 0.0001 | 0.0014 | 0 | 0 | 0 | 0.0003 | 0.0004 | 0.0002 | 0 | 0.0001 | 0 | – | – | – | – | – | – |
Bud Neck | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | – | – | – | – | – | – |
Bud Periphery | 0.0001 | 0.0001 | 0.001 | 0 | 0 | 0 | 0.0005 | 0.0002 | 0.0004 | 0 | 0.0002 | 0 | – | – | – | – | – | – |
Bud Site | 0.0007 | 0.0004 | 0.0022 | 0 | 0 | 0 | 0.0004 | 0.0004 | 0.0002 | 0 | 0.0004 | 0 | – | – | – | – | – | – |
Cell Periphery | 0.0001 | 0.0003 | 0.0017 | 0.0001 | 0 | 0 | 0.0026 | 0.0005 | 0.0003 | 0 | 0 | 0.0001 | – | – | – | – | – | – |
Cytoplasm | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0248 | 0.0039 | 0.0292 | 0.0003 | 0.0001 | 0 | 0.0025 | 0.0194 | 0.0035 | 0.0006 | 0.0027 | 0.0138 | – | – | – | – | – | – |
Eisosomes | 0.0006 | 0.0112 | 0.0012 | 0.0013 | 0.0001 | 0.0003 | 0.0012 | 0.0023 | 0.0034 | 0.0004 | 0.0005 | 0.0001 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Endosome | 0.0001 | 0 | 0.0015 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0.0005 | 0 | – | – | – | – | – | – |
Golgi | 0.0002 | 0.0001 | 0.0026 | 0.0004 | 0 | 0.0001 | 0.0007 | 0.0001 | 0.0033 | 0.0003 | 0.0021 | 0.0002 | – | – | – | – | – | – |
Lipid Particles | 0.008 | 0.0009 | 0.0132 | 0.0025 | 0.0001 | 0.0016 | 0.0204 | 0.0029 | 0.0196 | 0.0126 | 0.0053 | 0.0073 | – | – | – | – | – | – |
Mitochondria | 0.8808 | 0.9561 | 0.8734 | 0.9811 | 0.9801 | 0.9962 | 0.9123 | 0.9418 | 0.9247 | 0.9556 | 0.9293 | 0.9665 | – | – | – | – | – | – |
None | 0.001 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0 | – | – | – | – | – | – |
Nuclear Periphery | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | – | – | – | – | – | – |
Nucleolus | 0.0385 | 0.0046 | 0.0227 | 0.0001 | 0 | 0 | 0.0267 | 0.0198 | 0.0049 | 0.0001 | 0 | 0.0004 | – | – | – | – | – | – |
Nucleus | 0.0023 | 0.0001 | 0.0014 | 0 | 0 | 0 | 0.0009 | 0.0014 | 0.0006 | 0 | 0 | 0 | – | – | – | – | – | – |
Peroxisomes | 0.0358 | 0.008 | 0.0322 | 0.01 | 0.0195 | 0.0006 | 0.0149 | 0.0051 | 0.0195 | 0.0272 | 0.0506 | 0.0016 | – | – | – | – | – | – |
Punctate Nuclear | 0.0038 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Vacuole | 0.0004 | 0.0004 | 0.0037 | 0.0001 | 0 | 0 | 0.0052 | 0.001 | 0.0016 | 0 | 0.0001 | 0.0005 | – | – | – | – | – | – |
Vacuole Periphery | 0.0015 | 0.0011 | 0.0045 | 0.0006 | 0 | 0.0006 | 0.0079 | 0.0038 | 0.013 | 0.0001 | 0.001 | 0.0093 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 102.9137 | 34.7761 | 39.4118 | 81.0524 | 39.4335 | 153.4014 | 101.97 | 117.5814 | 164.9846 | 69.0036 |
Translational Efficiency | 1.6167 | 1.8261 | 1.58 | 1.7008 | 1.4138 | 1.8362 | 1.4757 | 1.6986 | 1.6306 | 1.5671 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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