Standard name
Human Ortholog
Description NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0.26 0.1 0.19 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.99 1.0 0.98 0.98 0.91 0.92 0.92 0.95 0.91 0.93 1.0 1.0 1.0 0.89 0.97 0.91 0.97 0.96 0.95 0.96 0.96 0.97
Endoplasmic Reticulum 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0 0 0.1 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 2 1 2 1 2 2 3 5 1 2 4 1 0 0 0 1 0 0 0 1 2 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 8 0 3 7 14 11 19 11 25 14 1 6 5 50 31 61 0 0 0 0 0 0
Cytoplasm 385 393 489 295 270 382 447 357 361 367 351 338 459 387 477 168 304 286 366 364 449 360 482 512
Endoplasmic Reticulum 2 0 0 0 2 5 33 24 16 12 16 13 0 0 1 5 32 20 0 0 6 2 1 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleus 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 1 0 1 1 3 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 2 2 1 3 4
Unique Cell Count 387 394 492 296 275 391 489 386 391 388 385 362 459 388 479 189 313 313 376 379 475 376 505 530
Labelled Cell Count 388 396 499 298 277 397 497 396 401 391 394 371 461 394 483 224 369 367 376 379 475 376 505 530


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 181.5 273.3 246.4 242.1 212.6 208.0 220.6 228.2 258.2 245.4 254.5 260.2 248.2 251.8 233.3 485.1 440.2 480.4 253.8 281.8 295.1
Std Deviation (1e-4) 37.4 52.5 58.2 48.4 62.9 54.4 58.0 64.6 72.5 72.6 76.8 83.8 54.5 56.9 59.1 132.6 118.6 122.7 66.1 76.4 78.0
Intensity Change (Log2) – – – -0.03 -0.21 -0.24 -0.16 -0.11 0.07 -0.01 0.05 0.08 0.01 0.03 -0.08 0.98 0.84 0.96 0.04 0.19 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.2 1.3 1.2 2.8 1.2 3.8 2.0 0 -0.1 -0.8 10.4 5.3 8.8
Cytoplasm 0.5 -1.6 -2.2 -6.0 -5.4 -5.5 -4.3 -6.0 -5.0 1.7 0.8 0.4 -6.7 -2.6 -5.9
Endoplasmic Reticulum 0 0 0 5.9 5.6 4.5 3.9 4.6 4.2 0 0 0 0 7.2 5.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 321.0419 372.806 317.3872 354.1447 310.0826 414.1222 337.8972 410.6541 380.5093 332.9919 355.3608 382.7136 361.4084 427.5338 343.3769 284.7338 325.4174 279.0001
Actin 0.003 0.0013 0.004 0.0022 0.0001 0.0001 0.0009 0.0002 0.0004 0.0002 0.0002 0.0003 0.0003 0.0003 0.0114 0.0101 0.0034 0.0013
Bud 0.0036 0.0003 0.0052 0.0002 0.0001 0.0001 0.0008 0.0005 0.0004 0.0003 0.0008 0.0005 0.0006 0.0005 0.0008 0.0007 0.0009 0.0005
Bud Neck 0.0002 0.0002 0.0003 0.0002 0.0003 0.0002 0.0002 0.0001 0.0002 0.0002 0.0004 0.0008 0.0001 0.0001 0.0003 0.0002 0.0002 0.0004
Bud Periphery 0.0011 0.0001 0.0085 0.0001 0 0 0.0002 0.0001 0.0002 0.0001 0.0003 0.0003 0.0002 0.0001 0.0003 0.0004 0.0006 0.0001
Bud Site 0.0015 0.0005 0.0018 0.0001 0 0 0.0004 0.0001 0.0001 0 0.0002 0.0001 0.0003 0.0001 0.0003 0.0002 0.0003 0.0003
Cell Periphery 0.0007 0.0005 0.0013 0.0001 0.0001 0 0.0013 0.0005 0.0004 0.0005 0.0015 0.0003 0.0005 0.0001 0.0005 0.0002 0.0009 0.0004
Cytoplasm 0.8325 0.9467 0.8314 0.8075 0.9652 0.9371 0.8505 0.9638 0.9425 0.9063 0.9037 0.9215 0.8876 0.9708 0.8575 0.6941 0.707 0.7255
Cytoplasmic Foci 0.0087 0.0003 0.0039 0.0002 0.0001 0.0001 0.0047 0.0004 0.0008 0.0015 0.0022 0.0016 0.0121 0.0002 0.0098 0.0008 0.0199 0.0122
Eisosomes 0.0004 0 0.0007 0 0 0 0.0014 0 0.0001 0.0001 0.0001 0.0001 0.0005 0 0.0007 0 0.0008 0.0001
Endoplasmic Reticulum 0.001 0.0003 0.0008 0.0011 0.0001 0 0.0006 0.0002 0.0005 0.0004 0.0005 0.0008 0.0002 0.0001 0.0027 0.0036 0.0005 0.0038
Endosome 0.0006 0 0.0008 0.0009 0.0001 0 0.0002 0 0.0002 0.0001 0.0002 0.0001 0.0001 0 0.0044 0.005 0.0019 0.0054
Golgi 0.0004 0 0.0003 0.0001 0 0 0.0001 0 0 0 0 0 0.0001 0 0.0011 0.0008 0.0003 0.0003
Lipid Particles 0.0037 0 0.001 0 0 0 0.0011 0 0.0003 0.0004 0.0007 0.0002 0.0012 0 0.0019 0.0002 0.005 0.0005
Mitochondria 0.0082 0.0019 0.0261 0.003 0.0004 0.0005 0.0046 0.0012 0.001 0.0003 0.0014 0.0036 0.0159 0.0006 0.0163 0.0073 0.0424 0.014
None 0.0071 0.0015 0.0075 0.0008 0.0001 0.0003 0.0232 0.0015 0.0036 0.0184 0.0124 0.0025 0.0161 0.0011 0.0082 0.0015 0.0227 0.0332
Nuclear Periphery 0.0323 0.0048 0.0131 0.0489 0.0056 0.0024 0.0182 0.003 0.0109 0.0087 0.015 0.0087 0.0058 0.0017 0.0096 0.1451 0.0317 0.0935
Nucleolus 0.0051 0.0128 0.014 0.0006 0.0001 0 0.0157 0.0042 0.0012 0.004 0.0049 0.0136 0.0149 0.0002 0.02 0.0015 0.0688 0.0237
Nucleus 0.0813 0.0274 0.0577 0.1312 0.0257 0.0588 0.069 0.0212 0.0347 0.0551 0.0415 0.0423 0.039 0.0234 0.0451 0.1167 0.0688 0.0531
Peroxisomes 0.0008 0 0.0009 0 0 0 0.0002 0 0 0 0.0001 0.0001 0.0007 0 0.0007 0.0001 0.0012 0.0004
Punctate Nuclear 0.0033 0.0003 0.0087 0.0008 0 0.0001 0.0018 0.0003 0.0004 0.0009 0.0008 0.0003 0.001 0.0001 0.0013 0.0022 0.0014 0.0206
Vacuole 0.0029 0.0005 0.0055 0.0006 0.001 0.0001 0.0034 0.0019 0.0014 0.002 0.0093 0.0015 0.0014 0.0003 0.0027 0.0022 0.0056 0.004
Vacuole Periphery 0.0016 0.0004 0.0066 0.0014 0.0011 0.0001 0.0014 0.0007 0.0007 0.0005 0.0039 0.0008 0.0015 0.0002 0.0045 0.0074 0.0155 0.0066

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 357.697 387.3243 419.8565 430.2582 575.389 300.5416 281.2965 278.127 356.7722 388.0319
Translational Efficiency 1.5471 1.3422 1.3016 1.4801 1.3931 1.3266 1.5087 1.3689 1.3107 1.4233

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Gdh1

Gdh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gdh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available