Standard name | |
---|---|
Human Ortholog | |
Description | DNA photolyase involved in photoreactivation; repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 0 | 1 | 3 | – | 5 | 4 | 5 | 2 | – | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 1 |
Bud Neck | 1 | 0 | 6 | 5 | 4 | 2 | 2 | – | 4 | 2 | 3 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 6 | 4 | 19 | 7 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 2 | 1 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 176 | 32 | 174 | 262 | 245 | 241 | 226 | – | 114 | 86 | 58 | 80 | – | 53 | 86 | 34 | 44 | 43 | 150 | 29 | 128 | 15 | 12 | 27 |
Endoplasmic Reticulum | 1 | 0 | 2 | 2 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 5 | 5 | 5 | 1 | 0 | 0 | 0 | 1 | 0 |
Endosome | 0 | 1 | 0 | 0 | 2 | 3 | 0 | – | 2 | 0 | 0 | 2 | – | 3 | 1 | 2 | 5 | 7 | 3 | 1 | 1 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 2 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 18 | 2 | 0 | 2 | 6 | 51 | 53 | – | 128 | 91 | 187 | 77 | – | 0 | 0 | 5 | 0 | 3 | 15 | 2 | 22 | 0 | 1 | 0 |
Nucleus | 25 | 32 | 30 | 25 | 91 | 194 | 309 | – | 263 | 338 | 343 | 382 | – | 30 | 50 | 76 | 92 | 43 | 37 | 54 | 29 | 1 | 11 | 11 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 2 | 4 | 0 | 3 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 2 | 3 | – | 9 | 14 | 23 | 19 | – | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 2 | 2 | 7 | – | 4 | 2 | 1 | 5 | – | 2 | 0 | 0 | 1 | 0 | 3 | 0 | 5 | 1 | 2 | 0 |
Vac/Vac Membrane | 12 | 12 | 5 | 4 | 25 | 35 | 16 | – | 13 | 13 | 11 | 9 | – | 9 | 7 | 44 | 55 | 48 | 28 | 14 | 37 | 3 | 8 | 13 |
Unique Cell Count | 222 | 71 | 210 | 295 | 359 | 489 | 575 | 462 | 493 | 489 | 522 | 90 | 142 | 147 | 179 | 136 | 260 | 109 | 239 | 24 | 43 | 61 | ||
Labelled Cell Count | 233 | 79 | 218 | 303 | 376 | 534 | 620 | 550 | 558 | 650 | 588 | 98 | 146 | 167 | 204 | 152 | 260 | 109 | 239 | 24 | 43 | 61 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.6 | 5.1 | 5.0 | 4.7 | 5.5 | 4.6 | 5.0 | – | 4.6 | 5.1 | 4.7 | 5.3 | – | 6.1 | 6.0 | 7.0 | 6.8 | 6.9 | – | – | 6.0 |
Std Deviation (1e-4) | 0.8 | 1.3 | 0.7 | 1.2 | 1.9 | 1.3 | 1.5 | – | 1.3 | 1.4 | 1.3 | 1.6 | – | 1.8 | 1.8 | 1.5 | 1.4 | 2.0 | – | – | 1.1 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.14 | -0.13 | -0.02 | – | -0.14 | 0.03 | -0.11 | 0.08 | – | 0.27 | 0.24 | 0.47 | 0.42 | 0.46 | – | – | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.9 | -3.8 | -8.3 | -10.8 | – | -14.1 | -16.4 | -18.3 | -17.4 | – | -4.4 | -4.7 | -11.3 | -11.5 | -9.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 4.6 | – | 0 | 6.7 | 0 | 5.9 | – | 0 | 0 | 0 | 0 | 0 |
Nucleus | -2.1 | 3.1 | 6.6 | 9.9 | – | 10.3 | 13.2 | 13.6 | 14.5 | – | 3.8 | 4.6 | 7.6 | 7.9 | 3.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.8 | – | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.4 | 2.5 | 0.3 | – | 0.3 | 0.2 | 0 | 0 | – | 0 | 0 | 7.4 | 7.7 | 8.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.1543 | -0.4411 | -0.6467 | -0.8893 | -1.07 | -0.5008 | -0.0778 | 0.1286 | 0.1877 | 0.1637 | 0.2591 | -0.0638 | -0.4317 | -0.7469 | -0.9916 | -0.9422 | -1.3226 | -0.573 |
Actin | 0.0521 | 0.0014 | 0.0025 | 0.0004 | 0.0042 | 0.0199 | 0.0144 | 0.0002 | 0.006 | 0.0357 | 0.0051 | 0.008 | 0.0343 | 0.0002 | 0.035 | 0.0003 | 0.0003 | 0.0142 |
Bud | 0.0018 | 0.0034 | 0.0009 | 0.0019 | 0.0001 | 0.0006 | 0.0005 | 0.0022 | 0.0004 | 0.0017 | 0.0008 | 0.001 | 0.0007 | 0.0001 | 0.0001 | 0.0013 | 0.0001 | 0.0009 |
Bud Neck | 0.0105 | 0.0003 | 0.0003 | 0.0004 | 0.0005 | 0.0021 | 0.0019 | 0.0004 | 0.0005 | 0.0029 | 0.0018 | 0.004 | 0.0033 | 0.0002 | 0.0006 | 0.0007 | 0.0015 | 0.0075 |
Bud Periphery | 0.0039 | 0.0029 | 0.0014 | 0.0034 | 0.0001 | 0.0013 | 0.0009 | 0.0013 | 0.0007 | 0.0081 | 0.002 | 0.0023 | 0.0008 | 0.0001 | 0.0002 | 0.0013 | 0.0001 | 0.0063 |
Bud Site | 0.0215 | 0.0057 | 0.0005 | 0.0003 | 0.0003 | 0.0004 | 0.0065 | 0.0036 | 0.0013 | 0.0017 | 0.0159 | 0.0004 | 0.0079 | 0.0018 | 0.0007 | 0.0009 | 0.0003 | 0.0004 |
Cell Periphery | 0.0012 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0008 |
Cytoplasm | 0.0512 | 0.1097 | 0.0719 | 0.0554 | 0.1484 | 0.1089 | 0.044 | 0.0651 | 0.0383 | 0.0101 | 0.0406 | 0.0729 | 0.0787 | 0.1085 | 0.1073 | 0.0702 | 0.1066 | 0.0788 |
Cytoplasmic Foci | 0.0288 | 0.0037 | 0.0062 | 0.0071 | 0.0079 | 0.0196 | 0.0247 | 0.0017 | 0.0046 | 0.0146 | 0.0184 | 0.0057 | 0.0383 | 0.0035 | 0.0074 | 0.0045 | 0.0024 | 0.0091 |
Eisosomes | 0.0009 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0092 | 0.0014 | 0.0027 | 0.0049 | 0.0064 | 0.0025 | 0.0036 | 0.0013 | 0.0021 | 0.0012 | 0.0037 | 0.0024 | 0.003 | 0.003 | 0.0032 | 0.0024 | 0.001 | 0.0149 |
Endosome | 0.0316 | 0.001 | 0.0063 | 0.003 | 0.0266 | 0.0048 | 0.0234 | 0.0008 | 0.0117 | 0.0256 | 0.0547 | 0.0107 | 0.0188 | 0.0021 | 0.0023 | 0.0009 | 0.0015 | 0.0035 |
Golgi | 0.0084 | 0.0001 | 0.0018 | 0.0002 | 0.003 | 0.003 | 0.0098 | 0 | 0.0049 | 0.0174 | 0.0079 | 0.0051 | 0.0085 | 0.0001 | 0.0031 | 0.0001 | 0.0001 | 0.0005 |
Lipid Particles | 0.0129 | 0.0003 | 0.0025 | 0.0019 | 0.0198 | 0.0016 | 0.0258 | 0.0003 | 0.0013 | 0.0086 | 0.0216 | 0.0027 | 0.0361 | 0.0007 | 0.0014 | 0.0012 | 0.0001 | 0.0005 |
Mitochondria | 0.0142 | 0.0004 | 0.0018 | 0.0013 | 0.0029 | 0.002 | 0.0101 | 0.0007 | 0.0101 | 0.0239 | 0.0087 | 0.0088 | 0.0036 | 0.0007 | 0.0024 | 0.0012 | 0.0004 | 0.0008 |
None | 0.3335 | 0.5826 | 0.4768 | 0.41 | 0.489 | 0.4037 | 0.4168 | 0.5424 | 0.4984 | 0.2905 | 0.4069 | 0.3375 | 0.4539 | 0.5581 | 0.5635 | 0.6563 | 0.5757 | 0.5278 |
Nuclear Periphery | 0.0484 | 0.0043 | 0.0262 | 0.0141 | 0.0463 | 0.0061 | 0.0131 | 0.0036 | 0.0083 | 0.0048 | 0.0449 | 0.0079 | 0.0116 | 0.0133 | 0.015 | 0.0026 | 0.0027 | 0.0076 |
Nucleolus | 0.0088 | 0.0029 | 0.0038 | 0.0064 | 0.0025 | 0.006 | 0.004 | 0.0057 | 0.0054 | 0.0043 | 0.0242 | 0.008 | 0.002 | 0.0034 | 0.0012 | 0.003 | 0.0015 | 0.0055 |
Nucleus | 0.3184 | 0.2712 | 0.3705 | 0.4717 | 0.2134 | 0.3869 | 0.3624 | 0.3582 | 0.3809 | 0.5108 | 0.3009 | 0.4862 | 0.2483 | 0.2892 | 0.242 | 0.2449 | 0.2925 | 0.3049 |
Peroxisomes | 0.0052 | 0.0003 | 0.0021 | 0.0004 | 0.0024 | 0.0014 | 0.0094 | 0.0001 | 0.0008 | 0.0073 | 0.006 | 0.0013 | 0.0334 | 0.0001 | 0.0029 | 0.0002 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0254 | 0.0066 | 0.018 | 0.0114 | 0.0197 | 0.0265 | 0.0231 | 0.0108 | 0.0179 | 0.0254 | 0.0221 | 0.0322 | 0.0135 | 0.0125 | 0.0096 | 0.0058 | 0.0127 | 0.0128 |
Vacuole | 0.0083 | 0.0011 | 0.0024 | 0.0044 | 0.004 | 0.0018 | 0.0037 | 0.0011 | 0.0047 | 0.0024 | 0.0086 | 0.0019 | 0.002 | 0.0018 | 0.0012 | 0.0018 | 0.0005 | 0.0024 |
Vacuole Periphery | 0.0037 | 0.0001 | 0.001 | 0.0013 | 0.0023 | 0.0005 | 0.0012 | 0.0001 | 0.0012 | 0.0025 | 0.0049 | 0.0008 | 0.001 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2.5993 | 0.5809 | 3.0174 | 5.1375 | 5.8977 | 9.742 | 4.552 | 7.9285 | 8.4841 | 5.9768 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|