Standard name
Human Ortholog
Description Eisosome core component; eisosomes are large immobile patch structures at the cell cortex associated with endocytosis; phosphorylated on Thr233 upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.34 0.53 0.23 0.22 0.15 0.18 0.14 0.31 0.27 0.36 0.37 0.39 0.08 0 0.08 0.19 0.2 0.26 0.39 0.34 0.22 0.21 0.1 0.05
Bud 0.1 0.12 0 0 0.05 0 0.06 0.1 0.07 0.1 0.06 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.23 0.57 0.32 0.33 0.26 0.24 0.22 0.2 0.23 0.31 0.29 0.29 0.19 0.29 0.42 0.62 0.62 0.57 0.37 0.52 0.49 0.34 0.15 0.09
Cytoplasm 0.45 0.19 0.61 0.65 0.7 0.55 0.44 0.31 0.22 0.19 0.14 0.08 0.86 0.92 0.85 0.4 0.4 0.43 0 0 0 0.25 0.6 0.73
Endoplasmic Reticulum 0 0.07 0 0 0 0.07 0 0.07 0.16 0.09 0.08 0.14 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0.12 0 0.05 0.07 0.3 0.43 0.39 0.56 0.61 0.66 0.56 0 0 0 0 0.06 0 0.05 0 0.1 0.1 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 58 80 31 25 27 44 38 87 61 84 65 71 14 10 26 51 35 60 86 60 37 35 30 15
Bud 17 18 6 2 9 10 17 27 15 23 11 15 4 0 3 3 3 3 0 0 0 0 1 2
Bud Neck 0 0 0 1 0 0 0 0 0 0 0 0 0 2 1 0 0 0 8 4 5 5 9 11
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 40 86 43 38 47 58 59 58 54 72 51 53 34 90 129 168 110 134 82 92 83 59 46 26
Cytoplasm 77 29 82 74 125 132 119 87 51 45 25 15 153 284 265 108 70 102 9 1 6 43 182 219
Endoplasmic Reticulum 6 11 6 0 4 17 9 20 37 22 14 25 6 2 1 10 9 11 5 3 2 3 6 7
Endosome 0 0 0 0 1 2 1 2 1 2 0 0 0 0 0 2 1 0 6 3 3 3 2 1
Golgi 0 0 1 0 0 0 0 0 0 2 0 0 0 0 0 1 0 2 1 1 0 0 0 1
Mitochondria 25 18 2 6 13 71 117 112 129 142 116 101 1 0 0 4 10 6 10 6 17 17 12 9
Nucleus 0 0 2 0 1 2 3 3 1 0 1 0 0 0 0 3 0 0 0 0 0 0 0 0
Nuclear Periphery 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 1 1 0 1 9 6 11 3 5 2 1 0 1 5 0 4 3 3 1 6 0 0 0
SpindlePole 3 0 7 4 2 3 1 3 0 0 0 0 0 4 9 3 3 7 2 0 0 0 2 0
Vac/Vac Membrane 0 0 0 0 0 1 4 2 0 3 0 1 0 0 1 3 4 5 1 1 2 1 1 3
Unique Cell Count 171 150 134 114 178 239 269 284 230 234 177 180 178 310 310 271 176 235 223 178 170 173 302 303
Labelled Cell Count 228 243 182 150 230 349 374 412 352 400 285 283 212 393 440 356 249 333 223 178 170 173 302 303


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 6.8 5.7 6.0 6.2 5.6 5.7 6.1 6.2 6.8 7.1 7.9 6.1 6.7 6.7 8.7 8.3 8.3 5.8 6.4 6.5
Std Deviation (1e-4) 0.5 1.0 0.9 1.5 1.4 1.0 1.2 1.0 1.2 1.2 1.5 1.7 0.8 1.1 1.0 1.3 1.5 1.6 1.0 1.6 1.4
Intensity Change (Log2) – – – 0.08 0.12 -0.02 -0.01 0.1 0.12 0.25 0.32 0.46 0.09 0.23 0.23 0.61 0.55 0.54 0.02 0.16 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.2 -1.8 -1.1 -2.3 1.6 0.7 2.5 2.6 3.1 -3.8 -6.7 -4.3 -1.0 -0.7 0.5
Bud 0 0.2 -0.1 0.8 1.8 0.8 1.8 0.7 1.4 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.2 -1.1 -1.6 -2.2 -2.6 -1.8 -0.3 -0.6 -0.5 -2.6 -0.6 1.9 5.7 5.3 4.6
Cytoplasm 0.6 1.7 -1.1 -3.2 -5.9 -7.5 -8.1 -8.7 -10.0 5.0 7.7 5.7 -4.0 -3.7 -3.3
Endoplasmic Reticulum 0 0 1.0 0 1.0 3.3 1.7 1.2 2.8 -0.5 0 0 -0.4 0.3 0.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.4 6.6 8.7 8.1 10.5 11.2 11.5 10.2 0 0 0 0 1.9 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -0.7 0 0 0 0 0 0 0 0 0 0 0 0 0 -1.1
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.4639 9.8806 8.649 7.5654 7.9228 8.4962 7.1694 8.6867 8.403 8.7392 7.2384 8.1693 11.0785 14.1186 12.0177 11.911 11.1554 11.3963
Actin 0.0771 0.0973 0.0643 0.0674 0.1074 0.0244 0.1134 0.164 0.1522 0.1354 0.1488 0.0575 0.063 0.0582 0.0364 0.1059 0.0368 0.0277
Bud 0.0019 0.0002 0.0002 0.0062 0.0002 0.0003 0.0002 0.0008 0.0004 0.0018 0.0003 0.0002 0.0002 0.0002 0.0001 0.0009 0.0001 0.0004
Bud Neck 0.0012 0.0025 0.004 0.0017 0.0039 0.0015 0.0044 0.005 0.0092 0.015 0.0036 0.0022 0.0014 0.0026 0.0037 0.0083 0.0022 0.0017
Bud Periphery 0.0041 0.0012 0.0018 0.0048 0.0021 0.005 0.0015 0.0028 0.0023 0.0067 0.0036 0.0034 0.0026 0.0008 0.001 0.0041 0.0016 0.0016
Bud Site 0.0052 0.0184 0.0071 0.0034 0.0022 0.0008 0.0073 0.0176 0.0168 0.0159 0.0021 0.0009 0.0051 0.0186 0.005 0.008 0.0023 0.0005
Cell Periphery 0.0866 0.0678 0.1244 0.0906 0.1735 0.1328 0.1079 0.0857 0.1049 0.096 0.2222 0.1795 0.0639 0.0619 0.0829 0.1086 0.0979 0.0929
Cytoplasm 0.0091 0.0183 0.0153 0.0263 0.0278 0.0116 0.0095 0.0161 0.03 0.0948 0.0204 0.0077 0.006 0.0038 0.0056 0.0162 0.0054 0.0059
Cytoplasmic Foci 0.0052 0.0021 0.0005 0.0006 0.0022 0.0006 0.0016 0.001 0.0007 0.0013 0.0011 0.0003 0.0008 0.0003 0.0002 0.0009 0.0002 0.0008
Eisosomes 0.7792 0.7355 0.7619 0.7603 0.6324 0.7939 0.7387 0.6806 0.6714 0.6114 0.5828 0.7381 0.8449 0.8435 0.8595 0.7273 0.8417 0.8598
Endoplasmic Reticulum 0.0047 0.0059 0.0052 0.0098 0.015 0.0058 0.0017 0.0025 0.0037 0.0033 0.0031 0.0044 0.0029 0.0027 0.0021 0.0093 0.0057 0.0031
Endosome 0.001 0.0011 0.0001 0.0003 0.0009 0.0005 0.001 0.0003 0.0001 0.0004 0.0002 0.0001 0.0008 0.0001 0 0.0005 0 0.0001
Golgi 0.0112 0.045 0.0124 0.0222 0.0263 0.0175 0.0029 0.0198 0.0063 0.015 0.003 0.0026 0.0024 0.0022 0.0018 0.0049 0.0047 0.003
Lipid Particles 0.0017 0.0009 0.0003 0.0007 0.0023 0.0004 0.0015 0.0003 0.0004 0.0008 0.001 0.0003 0.0008 0.0001 0.0002 0.0004 0.0003 0.0004
Mitochondria 0.0046 0.0023 0.0008 0.0023 0.0011 0.003 0.0008 0.0023 0.0005 0.0004 0.0029 0.0011 0.002 0.0042 0.0005 0.0032 0.0001 0.0005
None 0.0042 0.0009 0.0007 0.0014 0.0015 0.0011 0.0043 0.0006 0.0008 0.0005 0.0015 0.0012 0.0017 0.0005 0.0005 0.0005 0.0005 0.0011
Nuclear Periphery 0.0004 0.0002 0.0002 0.0004 0.0004 0.0002 0.0007 0.0002 0.0002 0.0003 0.0002 0.0002 0.0003 0.0002 0.0002 0.0003 0.0003 0.0002
Nucleolus 0.0003 0 0 0.0001 0.0001 0 0.0002 0 0 0 0 0 0.0001 0 0 0 0 0
Nucleus 0.0005 0 0 0.0002 0.0001 0 0.0002 0 0 0.0001 0 0 0.0001 0 0 0.0001 0 0
Peroxisomes 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0008 0.0002 0.0001 0.0001 0.0027 0.0001 0.0004 0.0001 0.0001 0.0001 0 0
Punctate Nuclear 0.0006 0 0 0 0 0 0.0012 0 0 0.0001 0.0001 0 0.0003 0 0 0 0 0
Vacuole 0.0007 0.0001 0.0003 0.0011 0.0005 0.0003 0.0002 0.0001 0.0001 0.0005 0.0001 0.0002 0.0002 0.0001 0.0001 0.0004 0.0001 0.0003
Vacuole Periphery 0.0001 0 0 0.0001 0.0001 0 0.0001 0 0 0 0 0 0 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 175.2972 137.4774 234.7039 237.3447 137.3443 231.0922 185.6617 228.3119 332.1886 153.6888
Translational Efficiency 1.8132 1.6878 1.5719 2.8551 2.077 1.8417 1.8188 2.4403 1.8824 2.0106

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Eisosome core component; eisosomes are large immobile patch structures at the cell cortex associated with endocytosis; phosphorylated on Thr233 upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family
Localization
Cell Percentages cell periphery (14%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-3

Lsp1

Lsp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lsp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available