Standard name
Human Ortholog
Description Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0.05 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.11 0 0.09 0.05 0.06 – 0.05 0.05 0 0.09 – – – 0.08 0 0.05 0 0 0 0 0 0.06
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.06 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.06 0 0
Mitochondria 0.08 0 0 0 0 0.05 0.07 – 0.21 0.13 0.3 0.2 – – – 0 0.06 0 0 0 0 0 0 0
Nucleus 0.96 0.97 0.77 0.95 0.87 0.86 0.8 – 0.7 0.75 0.68 0.7 – – – 0.75 0.82 0.77 0.91 0.95 0.7 0.69 0.71 0.69
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.06 0 0.11 0.17 – 0.25 0.23 0.4 0.23 – – – 0 0 0.05 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.06 0 0 0 – 0 0.05 0 0.05 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.11 0 0 0 0 – 0 0 0 0 – – – 0.11 0.11 0.11 0 0 0.09 0 0.11 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 2 0 0 0 0 1 0 0 0
Bud 0 0 0 0 0 0 0 – 1 1 2 0 – – – 1 0 1 1 1 0 0 1 0
Bud Neck 0 0 0 0 1 0 0 – 1 0 1 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 2 4 – 4 3 1 13 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 7 4 4 7 18 13 19 – 11 12 6 22 – – – 17 11 10 2 0 2 0 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – – – 6 5 5 0 1 0 0 0 0
Endosome 0 1 0 0 1 4 2 – 0 0 0 1 – – – 3 5 7 1 1 2 0 0 0
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 – – – 0 0 1 1 0 0 0 0 0
Mitochondria 19 0 0 6 1 13 21 – 44 31 45 50 – – – 10 16 3 1 1 0 0 0 0
Nucleus 220 218 27 149 166 214 236 – 145 183 103 180 – – – 169 230 141 214 216 42 11 26 30
Nuclear Periphery 0 0 0 0 0 0 0 – 0 4 1 1 – – – 1 0 0 0 0 0 0 0 0
Nucleolus 9 1 0 9 7 28 50 – 53 57 61 58 – – – 6 5 9 1 1 1 0 1 2
Peroxisomes 0 0 0 0 1 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 10 3 5 8 – 9 11 0 14 – – – 4 3 3 0 1 0 0 0 1
Vac/Vac Membrane 0 5 4 1 0 2 3 – 1 3 2 2 – – – 24 30 20 6 2 5 0 4 2
Unique Cell Count 230 225 35 157 191 248 296 208 244 151 256 224 281 184 236 229 61 17 37 45
Labelled Cell Count 255 230 35 182 198 281 343 269 305 222 341 243 305 200 236 229 61 17 37 45


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 7.3 8.6 6.3 7.3 6.2 6.3 – 5.4 5.7 4.8 5.3 – – – 8.9 8.1 8.2 – – –
Std Deviation (1e-4) 0.8 1.1 3.4 1.3 2.3 1.7 1.7 – 1.5 1.6 0.9 1.6 – – – 1.8 1.5 1.6 – – –
Intensity Change (Log2) – – – -0.45 -0.24 -0.48 -0.46 – -0.68 -0.59 -0.84 -0.69 – – – 0.05 -0.09 -0.08 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 – 3.0 0 0 2.9 – – – 0 0 0
Nucleus 3.4 1.5 1.4 0.4 – -0.9 -0.3 -1.0 -0.8 – – – -0.2 0.7 -0.1
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 2.6 – 3.4 3.2 0 3.1 – – – 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7121 1.1558 1.2946 1.0158 1.1405 1.213 0.8577 0.7232 0.9085 0.6979 0.4107 0.8981 0.389 0.2848 0.1726 0.2694 -0.0399 0.232
Actin 0.014 0 0.0017 0 0.0109 0.0018 0.027 0.0018 0.0216 0.0377 0 0.0004 0.0125 0 0.0009 0.0374 0.0002 0.0006
Bud 0.0007 0.0011 0.0002 0 0.0009 0.0005 0.0003 0.0001 0.0003 0.0008 0 0.0001 0.0004 0 0.0002 0.0014 0.0001 0.0009
Bud Neck 0.0067 0.0001 0.0005 0.0001 0.0153 0.0008 0.0006 0.0002 0.0003 0.001 0.0006 0.0007 0.0034 0.0005 0.0004 0.0014 0.0071 0.0007
Bud Periphery 0.0012 0.0002 0.0004 0 0.0045 0.0002 0.0004 0.0002 0.0003 0.0034 0 0.0001 0.0005 0 0.0003 0.0019 0.0001 0.0021
Bud Site 0.007 0.0009 0.0005 0 0.002 0.0001 0.0029 0.0015 0.0076 0.0024 0.0001 0.0001 0.0056 0.0002 0.0019 0.0126 0.0002 0.0025
Cell Periphery 0.0004 0.0001 0.0001 0 0.0007 0 0.0002 0.0002 0.0001 0.0009 0 0 0.0002 0 0.0001 0.0005 0 0
Cytoplasm 0.0032 0.0037 0.0005 0 0.0006 0.0022 0.004 0.0023 0.004 0.0013 0.0001 0.0001 0.0024 0.0004 0.0066 0.0039 0.0004 0.0001
Cytoplasmic Foci 0.0077 0.0006 0.0031 0 0.0049 0.0015 0.0167 0.0002 0.0068 0.0059 0 0.0017 0.0099 0 0.0014 0.0261 0 0.0016
Eisosomes 0.0002 0 0.0001 0 0.0002 0.0001 0.0003 0.0011 0.0002 0.0016 0 0 0.0002 0 0.0001 0.0006 0 0
Endoplasmic Reticulum 0.0022 0.0001 0.0005 0 0.0013 0.0002 0.0017 0.0003 0.0029 0.0012 0 0.0001 0.0019 0 0.0008 0.0066 0.0002 0
Endosome 0.0146 0.0001 0.0035 0 0.0142 0.0041 0.0226 0.0004 0.0057 0.0045 0 0.0061 0.017 0.0002 0.0056 0.0316 0.0002 0.0007
Golgi 0.0066 0 0.0021 0 0.0089 0.0044 0.0051 0.0001 0.0066 0.0075 0 0.0179 0.0024 0 0.0003 0.0168 0 0.0005
Lipid Particles 0.0151 0 0.0049 0 0.0133 0.0033 0.029 0.0003 0.01 0.008 0 0.0038 0.0106 0 0.0004 0.041 0 0.0019
Mitochondria 0.0116 0.0003 0.0065 0.0001 0.0129 0.0074 0.0071 0.0037 0.0086 0.0179 0.0003 0.0039 0.004 0.0002 0.0006 0.0282 0.0003 0.0031
None 0.0019 0.0009 0.0008 0 0.0005 0.0002 0.0175 0.0102 0.0016 0.0016 0 0.0001 0.004 0.0001 0.0036 0.0035 0.0001 0.0002
Nuclear Periphery 0.0223 0.0027 0.0037 0.0002 0.0095 0.0019 0.0181 0.0055 0.0026 0.001 0.0021 0.0006 0.0064 0.0018 0.0033 0.0041 0.005 0.0002
Nucleolus 0.0255 0.0172 0.0184 0.0143 0.0632 0.0403 0.0124 0.0181 0.0206 0.0261 0.0722 0.0218 0.0216 0.0203 0.0268 0.0046 0.0669 0.0437
Nucleus 0.8174 0.9501 0.9369 0.9592 0.7956 0.9065 0.7973 0.9424 0.891 0.861 0.9142 0.9389 0.8658 0.9744 0.9236 0.7463 0.9022 0.9359
Peroxisomes 0.0046 0 0.005 0 0.0045 0.0026 0.0193 0.0003 0.0067 0.0112 0 0.0011 0.0034 0 0.0003 0.0246 0 0.0035
Punctate Nuclear 0.031 0.0212 0.009 0.026 0.0299 0.0214 0.011 0.0109 0.0014 0.0009 0.01 0.0017 0.0258 0.0014 0.0163 0.0038 0.0166 0.0012
Vacuole 0.0036 0.0004 0.0011 0 0.0039 0.0002 0.0054 0.0002 0.0007 0.003 0.0001 0.0005 0.0014 0.0003 0.0046 0.0021 0.0002 0.0003
Vacuole Periphery 0.0026 0.0002 0.0005 0 0.0024 0.0002 0.0011 0.0001 0.0006 0.0013 0 0.0004 0.0006 0.0001 0.002 0.0011 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.8646 11.1644 5.3849 10.6593 12.954 17.4749 9.1718 11.8092 9.6442 15.2051
Translational Efficiency 0.7359 0.5774 0.7723 0.4682 0.4354 0.5454 0.6131 0.4212 0.5115 0.5358

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Tfc8

Tfc8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tfc8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available