Standard name
Human Ortholog
Description Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.43 0.55 0.85 0.78 0.77 0.7 0.63 0.59 0.51 0.5 0.35 0.44 0.53 0.55 0.7 0.54 0.47 0.45 0.22 0.39 0.42 0.31 0.26 0.35
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.29 0 0.13 0 0.29 0.34 0.24 0.54 0.48 0.66 0.5 0.13 0 0 0 0 0 0 0.08 0.05 0.09 0.13 0.11
Nucleus 0.14 0.1 0 0.06 0.1 0.07 0.13 0.16 0.13 0.14 0.15 0.17 0.13 0.06 0 0.1 0.24 0.15 0.34 0.16 0.1 0.12 0.07 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.36 0.11 0.11 0.05 0.12 0.06 0 0.07 0.05 0.06 0 0.07 0.31 0.45 0.35 0.39 0.31 0.44 0.29 0.28 0.32 0.38 0.38 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 2 0 0 0 1 0 0 0 0 0 0 0 1 0 3 6 8
Bud 0 1 0 0 0 1 2 5 4 2 11 3 0 0 1 1 0 0 4 1 4 0 15 8
Bud Neck 0 2 6 7 11 4 3 23 7 3 2 7 0 0 0 1 0 0 0 1 1 1 4 6
Bud Site 0 0 0 0 0 1 2 0 5 3 8 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 1 0 0 1 1 0 1 0 0 0 0 0 0 1 0 0 0 0 1 0
Cytoplasm 88 80 204 189 215 180 251 249 198 180 106 131 120 160 254 126 104 111 57 82 132 65 108 127
Endoplasmic Reticulum 4 6 0 0 1 0 0 1 0 0 0 0 0 0 0 16 11 13 1 0 1 1 3 8
Endosome 1 0 0 1 0 0 0 1 1 0 0 0 2 11 7 9 4 14 6 0 1 2 5 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 0 0 0 4 1
Mitochondria 39 42 9 31 10 74 134 102 210 172 200 150 29 13 12 6 5 5 11 16 17 19 52 39
Nucleus 29 14 5 14 27 19 53 69 50 51 46 50 29 18 10 24 52 36 87 33 30 24 28 13
Nuclear Periphery 2 0 0 0 0 0 0 0 1 0 2 0 0 0 1 0 1 0 1 0 1 0 0 0
Nucleolus 1 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
SpindlePole 0 0 1 4 3 2 11 8 4 2 6 7 0 0 0 2 0 0 7 5 8 3 9 7
Vac/Vac Membrane 73 16 26 11 33 15 13 30 18 20 9 21 71 130 128 91 68 108 74 59 102 81 158 118
Unique Cell Count 203 145 240 243 278 258 397 419 390 362 304 299 228 291 363 234 219 246 261 213 316 214 413 363
Labelled Cell Count 237 162 252 259 301 296 469 491 499 434 391 380 251 332 413 277 246 291 261 213 316 214 413 363


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.3 4.7 4.2 4.9 3.7 3.9 4.0 3.5 3.4 3.0 3.6 4.1 4.1 4.3 6.0 6.0 5.8 3.9 4.2 4.4
Std Deviation (1e-4) 1.0 0.5 1.2 1.3 1.5 1.4 1.1 1.2 2.0 1.3 0.7 0.6 1.3 1.0 1.3 1.8 1.3 1.2 1.2 2.2 1.8
Intensity Change (Log2) – – – -0.15 0.07 -0.34 -0.27 -0.21 -0.44 -0.47 -0.66 -0.38 -0.18 -0.19 -0.12 0.36 0.35 0.3 -0.26 -0.17 -0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 7.1 5.4 8.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6048 1.8065 2.1197 0.6866 1.0019 1.7788 -0.94 -0.7744 -0.8169 -0.775 -0.9968 -0.8968 0.315 -0.696 -0.7216 -0.2905 -1.2024 -0.9106
Actin 0.0313 0.0147 0.0107 0.0008 0.0146 0.0014 0.0302 0.0002 0.0024 0.0106 0.0036 0.0021 0.0498 0.0032 0.0124 0.0682 0.0001 0.0278
Bud 0.0002 0.0001 0.0008 0.0001 0.0003 0.0001 0.0018 0.0045 0.0001 0.0002 0.0022 0.0005 0.0021 0.0017 0.0014 0.0004 0.0001 0.0003
Bud Neck 0.0138 0.0004 0.0013 0.0011 0.001 0.0042 0.0027 0.0003 0.0011 0.0006 0.0016 0.0087 0.0132 0.0004 0.0029 0.0015 0.001 0.0023
Bud Periphery 0.0004 0.0001 0.0024 0.0002 0.0007 0.0002 0.0015 0.0005 0.0001 0.0002 0.0033 0.0013 0.0032 0.0035 0.0014 0.0005 0.0001 0.0004
Bud Site 0.0053 0.0017 0.0054 0.0002 0.0034 0.0002 0.0085 0.0014 0.0015 0.0004 0.0044 0.0007 0.0138 0.0088 0.0063 0.0011 0.0002 0.0003
Cell Periphery 0.0002 0.0004 0.0033 0.0001 0.0004 0.0003 0.0005 0.0001 0.0001 0.0001 0.0001 0.0001 0.0009 0.0003 0.0003 0.0001 0 0.0001
Cytoplasm 0.1684 0.1607 0.1196 0.2282 0.2293 0.2198 0.1912 0.2656 0.1859 0.1594 0.2304 0.2306 0.1994 0.2764 0.2724 0.1362 0.2628 0.2667
Cytoplasmic Foci 0.0118 0.0057 0.0261 0.003 0.0113 0.0118 0.032 0.005 0.0126 0.01 0.0149 0.0168 0.0495 0.0067 0.0107 0.021 0.0049 0.0247
Eisosomes 0.0009 0.0002 0.0007 0.0001 0.0016 0.0002 0.0004 0.0001 0.0001 0.0001 0.0001 0 0.0007 0.0002 0.0002 0.0002 0 0.0001
Endoplasmic Reticulum 0.0051 0.0062 0.005 0.0037 0.0033 0.0032 0.0055 0.0041 0.0044 0.0022 0.0034 0.0027 0.0125 0.0098 0.0062 0.0126 0.0022 0.0074
Endosome 0.006 0.0021 0.0044 0.0028 0.0241 0.0043 0.0351 0.0038 0.0148 0.0273 0.0231 0.0072 0.05 0.0053 0.0185 0.028 0.0022 0.0126
Golgi 0.0027 0.0002 0.0026 0.0001 0.0118 0.0006 0.0059 0.0001 0.0014 0.0148 0.0139 0.0026 0.0197 0.0004 0.005 0.0172 0 0.0048
Lipid Particles 0.0136 0.0025 0.011 0.0009 0.0493 0.0086 0.0166 0.0005 0.006 0.0093 0.0051 0.0044 0.0341 0.0013 0.0025 0.0105 0.0005 0.0051
Mitochondria 0.0067 0.0005 0.0185 0.0019 0.0633 0.0012 0.0174 0.0007 0.002 0.0053 0.0062 0.002 0.0185 0.0046 0.0058 0.0046 0.0003 0.0025
None 0.5481 0.6278 0.628 0.5651 0.3404 0.4846 0.3325 0.4695 0.3904 0.3768 0.3768 0.2463 0.3669 0.4489 0.3811 0.3761 0.3879 0.2744
Nuclear Periphery 0.0153 0.0063 0.0186 0.0198 0.0046 0.0056 0.0153 0.0142 0.0277 0.0118 0.0173 0.0089 0.023 0.0254 0.0191 0.0289 0.0064 0.0184
Nucleolus 0.0015 0.0013 0.0022 0.0022 0.0015 0.003 0.0049 0.0021 0.0086 0.0034 0.004 0.0049 0.0022 0.002 0.0023 0.0012 0.0017 0.0056
Nucleus 0.1441 0.1627 0.1097 0.1536 0.1852 0.2174 0.2157 0.2134 0.3036 0.3388 0.2468 0.3934 0.0872 0.1769 0.2147 0.2745 0.3038 0.281
Peroxisomes 0.0165 0.0008 0.021 0.0004 0.0428 0.0063 0.0309 0.0001 0.0042 0.0044 0.0039 0.015 0.0216 0.0008 0.0012 0.0031 0.0001 0.0011
Punctate Nuclear 0.0062 0.0037 0.0054 0.0125 0.0055 0.025 0.039 0.0112 0.029 0.0193 0.0328 0.0498 0.0195 0.0158 0.0295 0.011 0.0244 0.0612
Vacuole 0.0016 0.0017 0.0024 0.0021 0.004 0.0018 0.0084 0.0021 0.0032 0.0034 0.0047 0.0017 0.0095 0.0065 0.0042 0.0018 0.001 0.0021
Vacuole Periphery 0.0006 0.0002 0.0008 0.0008 0.0015 0.0003 0.0039 0.0004 0.0009 0.0016 0.0014 0.0004 0.0027 0.0015 0.0018 0.0013 0.0002 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.2272 8.7729 7.359 11.136 12.1707 8.529 8.581 8.0692 9.9187 12.9302
Translational Efficiency 0.3489 0.3726 0.4224 0.3628 0.2657 0.5911 0.4796 0.3802 0.2438 0.3064

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome
Localization
Cell Percentages nucleus (16%), cytoplasm (6%), mixed (56%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.00082)
Subcompartmental Group N/A

Chl1

Chl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Chl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available