Standard name
Human Ortholog
Description Cytoplasmic NAD(+)-dependent protein deacetylase; deacetylation targets are primarily cytoplasmic proteins; member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.11 0.1 0.11 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.96 0.96 0.94 0.88 0.76 0.76 0.75 0.77 0.6 0.72 0.99 0.99 0.99 0.91 0.94 0.95 0.95 0.95 0.91 0.97 0.9 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.14 0.28 0.26 0.33 0.26 0.5 0.32 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 3 7 4 1 2 0 0 0 0 0 0 1 0 0 0 0 1
Bud 1 1 2 0 0 0 4 7 11 11 12 8 0 0 0 0 0 0 0 0 0 0 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 5 5 4 9 19 9 6 8 11 12 12 0 8 5 24 33 27 0 0 0 0 0 0
Cytoplasm 386 393 230 204 322 300 336 270 254 337 210 207 183 198 239 201 304 232 380 385 227 127 244 205
Endoplasmic Reticulum 2 9 5 3 6 4 5 5 10 5 3 5 0 1 1 5 9 9 5 7 6 1 7 4
Endosome 0 0 0 1 0 0 2 4 2 1 0 1 0 0 0 0 2 1 3 0 1 0 3 6
Golgi 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 3 2 3 0 2 1 0 1 1
Mitochondria 1 1 2 1 17 49 124 91 111 113 176 91 2 0 0 2 3 2 0 2 6 0 3 0
Nucleus 0 1 2 0 1 3 6 3 3 3 4 0 0 0 1 0 2 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 0 6 12 10 20 13 11 11 13 11 0 0 0 12 18 5 0 3 5 0 3 0
Unique Cell Count 389 404 239 213 344 339 441 355 337 439 350 288 184 200 241 221 322 243 399 406 251 131 270 226
Labelled Cell Count 391 412 247 219 367 385 506 404 417 498 431 338 185 207 246 248 374 279 399 406 251 131 270 226


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 10.9 8.4 8.5 7.8 6.6 6.2 6.6 5.9 6.3 5.4 6.0 9.2 9.7 9.4 14.6 15.2 15.4 9.1 9.4 10.0
Std Deviation (1e-4) 1.0 1.5 1.1 1.7 1.3 1.2 1.2 1.2 1.2 1.1 1.0 0.9 1.6 1.3 1.3 2.2 2.1 2.2 1.4 1.6 1.7
Intensity Change (Log2) – – – 0.02 -0.1 -0.34 -0.44 -0.35 -0.51 -0.42 -0.62 -0.48 0.13 0.21 0.17 0.8 0.86 0.88 0.11 0.17 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1.9 0 2.0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.4 2.1 0 0 0.2 0.3 1.0 1.3 0 1.2 0 3.9 3.8 4.0
Cytoplasm -0.2 -1.4 -3.3 -6.7 -6.6 -6.7 -6.5 -9.9 -7.4 2.2 1.8 2.1 -2.3 -1.0 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0.6 0 0 0 0 0 0 0 0.5 1.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.7 5.7 8.7 8.2 9.6 8.3 12.8 9.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.3 3.0 0 0 3.0 0 0 0 0 3.7 3.7 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.7078 7.6124 7.035 6.9873 5.9979 7.3069 5.3884 7.0437 6.4214 6.2024 6.3673 6.4094 4.9996 6.3129 5.9185 5.4103 5.3801 6.1636
Actin 0.0193 0.0001 0.0128 0.0028 0.0014 0.0004 0.0033 0.0012 0.0006 0.0002 0.0023 0.0009 0.0208 0.0002 0.0068 0.0043 0.0423 0.0008
Bud 0.0005 0.0002 0.0006 0.0004 0.0005 0.0001 0.0008 0.0016 0.0023 0.0016 0.0007 0.0013 0.0006 0.0002 0.0003 0.0004 0.0016 0.0002
Bud Neck 0.0044 0.0002 0.002 0.0006 0.0006 0.0008 0.001 0.0003 0.0006 0.001 0.0006 0.003 0.0005 0.0003 0.0003 0.0004 0.0037 0.0009
Bud Periphery 0.0007 0 0.0006 0.0003 0.0005 0.0001 0.0006 0.0004 0.0015 0.001 0.0009 0.001 0.0008 0.0001 0.0006 0.0002 0.0012 0.0001
Bud Site 0.0075 0.0015 0.0017 0.0007 0.0007 0.0001 0.0096 0.0085 0.0074 0.0003 0.0001 0.0011 0.0031 0.004 0.0012 0.0005 0.0054 0.0001
Cell Periphery 0.0007 0.0001 0.0003 0.0001 0.0001 0.0001 0.0003 0.0003 0.0002 0.0001 0 0.0002 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.7901 0.9697 0.8993 0.8496 0.7996 0.9487 0.8813 0.9402 0.9266 0.931 0.9128 0.9259 0.7213 0.9595 0.8925 0.7589 0.7372 0.9025
Cytoplasmic Foci 0.0302 0.0084 0.0155 0.0163 0.0397 0.0112 0.0142 0.0132 0.0149 0.0137 0.0097 0.0149 0.0409 0.0059 0.0169 0.0577 0.0415 0.0079
Eisosomes 0.0004 0 0.0002 0.0001 0 0 0 0 0 0 0 0 0.0003 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0303 0.0051 0.0098 0.0106 0.005 0.0135 0.0257 0.0054 0.0062 0.0083 0.004 0.0134 0.0488 0.0031 0.0125 0.008 0.009 0.0436
Endosome 0.0527 0.0082 0.0261 0.0356 0.0582 0.0152 0.035 0.0137 0.0195 0.0164 0.0129 0.0208 0.0459 0.0132 0.0261 0.0664 0.083 0.0243
Golgi 0.0084 0.0007 0.0051 0.0038 0.0236 0.004 0.0024 0.0009 0.0009 0.0005 0.0059 0.0014 0.0104 0.001 0.0049 0.0604 0.0243 0.003
Lipid Particles 0.0092 0.0001 0.0039 0.001 0.0016 0.0002 0.001 0.0001 0.0002 0 0.0001 0.0002 0.0115 0.0001 0.0007 0.0047 0.0103 0.0058
Mitochondria 0.0081 0.0004 0.0035 0.039 0.0293 0.0011 0.0014 0.0005 0.0006 0.0003 0.0166 0.0021 0.0211 0.0002 0.0122 0.0051 0.0044 0.0023
None 0.0156 0.0017 0.0064 0.008 0.0255 0.0012 0.0108 0.0045 0.0068 0.0165 0.0021 0.005 0.0191 0.0068 0.0056 0.0014 0.0023 0.001
Nuclear Periphery 0.0029 0.0004 0.0018 0.0032 0.0013 0.0005 0.0024 0.0005 0.0012 0.0009 0.0189 0.0011 0.018 0.0006 0.0032 0.0015 0.0083 0.0012
Nucleolus 0.0005 0 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0002 0 0.0002 0.0001 0.0018 0 0.0001 0 0.0001 0
Nucleus 0.0011 0.0007 0.001 0.0014 0.0021 0.0007 0.0018 0.0018 0.0022 0.0017 0.0079 0.0023 0.007 0.0007 0.001 0.001 0.0034 0.0009
Peroxisomes 0.0086 0.0001 0.0026 0.0008 0.0022 0.0002 0.0003 0.0001 0.0002 0.0001 0.0006 0.0002 0.0095 0 0.0013 0.0139 0.0107 0.0006
Punctate Nuclear 0.0009 0.0001 0.0009 0.0003 0.0006 0.0001 0.0007 0.0018 0.0012 0.0002 0.0003 0.0006 0.0085 0.0001 0.0002 0.0014 0.004 0.0001
Vacuole 0.006 0.0018 0.0047 0.0222 0.0057 0.0016 0.0063 0.0046 0.0057 0.0052 0.0023 0.0037 0.0069 0.0035 0.0108 0.0113 0.0052 0.0031
Vacuole Periphery 0.0018 0.0003 0.001 0.003 0.0017 0.0004 0.0009 0.0005 0.0009 0.0011 0.0011 0.0008 0.0028 0.0004 0.0023 0.0025 0.002 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.9647 38.5734 70.1948 53.3961 59.2758 57.5013 46.4304 55.7805 67.5579 52.7088
Translational Efficiency 0.4444 0.4278 0.3795 0.5466 0.2909 0.4685 0.496 0.6048 0.4573 0.4684

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytoplasmic NAD(+)-dependent protein deacetylase; deacetylation targets are primarily cytoplasmic proteins; member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Hst2

Hst2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hst2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available