Standard name
Human Ortholog
Description Outer kinetochore protein, needed for accurate chromosome segregation; component of kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as platform for kinetochore assembly; required for spindle assembly checkpoint; minimizes potentially deleterious centromere-proximal crossovers by preventing meiotic DNA break formation proximal to centromere; homolog of human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.07 0.06 0.08 0.07 0.07 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.11 0 0.08 0.05 0 0.09 0.05 0.07 0.05 0.09 0.06 0.05 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0.08 0.09 0.09 0.08 0.12 0.09 0.06 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0.12 0.12 0.1 0.08 0.06 0.07 0.05 0.06 0.06 0.05 0 0.22 0.25 0.1 0.09 0.08 0 0 0 0 0 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.07 0.28 0 0.11 0 0.07 0 0 0 0 0 0 0 0.06 0.21 0.35 0.44 0.53 0.1 0.12 0.09 0.08 0.06 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.36 0.12 0.1 0 0 0.15 0.2 0.13 0.3 0.26 0.4 0.28 0.14 0 0 0.07 0 0.05 0 0 0 0 0 0
Nucleus 0.06 0.09 0.07 0.07 0.05 0.06 0.05 0.06 0.05 0.07 0.07 0.06 0.06 0.05 0.08 0.09 0.07 0.05 0 0 0 0.06 0.06 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.5 0.56 0.21 0.25 0.24 0.22 0.3 0.3 0.31 0.27 0.33 0.26 0.37 0.17 0.18 0.39 0.42 0.38 0.05 0.08 0 0 0.06 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.59 0.51 0.75 0.74 0.81 0.74 0.7 0.76 0.64 0.65 0.62 0.67 0.57 0.64 0.53 0.66 0.56 0.47 0.74 0.66 0.73 0.7 0.69 0.6
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0.06 0.09 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 0 0 0 2 2 0 4 2 1 0 0 0 1 0 0 0 0 0 2
Bud 2 2 4 1 11 12 34 27 35 28 26 34 2 0 1 1 2 1 1 3 2 1 4 3
Bud Neck 1 0 21 7 29 17 21 42 21 27 17 44 2 5 4 0 0 0 0 1 0 0 1 3
Bud Site 7 1 7 5 14 19 39 41 37 33 43 42 2 1 0 2 5 6 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 7 23 30 36 30 31 31 21 23 20 23 1 24 33 26 28 19 0 2 8 1 4 12
Endoplasmic Reticulum 1 0 0 1 1 2 1 0 1 0 0 0 0 0 0 6 5 3 0 1 1 0 0 1
Endosome 11 57 5 28 14 24 15 3 7 2 2 8 1 7 28 90 142 132 17 29 22 6 13 16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 2 1 0 0 0 0
Mitochondria 58 25 19 6 9 55 104 60 125 105 142 133 5 1 1 18 13 12 1 5 2 0 5 4
Nucleus 9 17 14 16 19 22 27 29 22 29 25 28 2 5 10 23 22 12 4 9 6 5 12 4
Nuclear Periphery 0 4 0 0 0 3 1 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0
Nucleolus 82 112 39 61 88 77 156 140 128 111 119 125 13 18 24 100 137 96 8 18 6 1 12 5
Peroxisomes 3 1 0 1 1 4 9 4 3 3 3 2 0 0 2 4 4 4 1 1 2 0 0 1
SpindlePole 96 101 141 181 293 266 362 357 266 263 220 321 20 70 71 169 181 118 129 157 175 59 150 103
Vac/Vac Membrane 3 7 0 2 0 1 2 3 0 1 1 2 3 1 2 15 29 31 5 3 6 2 5 6
Unique Cell Count 163 200 189 245 361 358 514 471 417 405 356 479 35 109 133 258 323 251 176 240 241 85 217 173
Labelled Cell Count 281 334 273 339 517 532 802 737 668 627 619 766 53 133 176 454 572 438 176 240 241 85 217 173


Ambiguous, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 5.4 4.7 5.1 5.0 4.4 4.1 4.4 3.8 3.9 3.6 3.9 4.6 5.5 5.9 7.4 7.5 7.1 4.8 5.3 5.9
Std Deviation (1e-4) 0.7 0.9 1.2 1.8 1.5 1.7 1.6 1.4 1.3 1.1 1.3 1.0 0.6 1.2 2.0 1.7 2.0 1.7 1.4 1.4 1.8
Intensity Change (Log2) – – – 0.12 0.09 -0.11 -0.21 -0.1 -0.32 -0.26 -0.39 -0.28 -0.03 0.21 0.33 0.65 0.67 0.59 0.03 0.17 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud 0 0.6 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck -3.5 -1.2 0 0 0 0 0 0 0 – -1.9 -2.7 -5.5 -6.1 -5.4
Bud Site -1.0 0.1 0 0 0 0 0 0 0 – 0 0 0 0 -0.8
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome 3.4 0.8 0 0 0 0 0 0 0 – 0 5.4 8.2 10.0 11.2
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria -3.4 -3.8 0 0 0 0 0 0 0 – -3.0 -3.4 -1.2 -2.7 -2.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus 1.0 1.0 0 0 0 0 0 0 0 – -0.9 -0.6 4.1 5.0 4.0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole -0.2 1.8 0 0 0 0 0 0 0 – -1.9 -4.0 -2.1 -4.2 -5.8
Vac – – – – – – – – – – – – – – –
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cytoplasm 0 -0.8 -1.4 -2.7 -2.4 -3.1 -2.8 -2.7 -3.4 – 2.2 2.9 -0.7 -1.3 -1.6
Nucleus -0.4 -1.0 -0.6 -1.1 -0.6 -1.0 -0.1 -0.2 -0.7 – -1.0 0 0.6 -0.3 -1.2
Vacuole 0 0 0 0 0 0 0 0 0 – 0 0 3.4 4.2 5.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2513 0.4787 0.5068 0.1689 0.0105 0.4165 0.2976 0.4008 0.35 0.1184 0.1983 0.4637 0.0538 -0.1094 -0.123 0.1884 -0.5183 -0.2661
Actin 0.0014 0 0.0001 0.0003 0.0001 0.0008 0.0053 0.0001 0.0004 0.0004 0.006 0.0014 0.0148 0.0003 0.0127 0.0026 0.0001 0.0006
Bud 0 0 0.0001 0 0 0 0.0025 0.0003 0.0001 0 0.0001 0 0.0003 0.0001 0.0002 0.0001 0 0.0001
Bud Neck 0.0005 0.0006 0.0003 0.0003 0.0003 0.0009 0.0012 0.0009 0.0036 0.001 0.0006 0.001 0.0023 0.001 0.0012 0.0036 0.0011 0.0052
Bud Periphery 0 0 0.0001 0 0 0 0.0014 0.0001 0.0001 0.0001 0.0002 0.0001 0.0004 0.0001 0.0003 0.0002 0.0001 0.0002
Bud Site 0.0003 0.0005 0.0007 0 0.0001 0.0001 0.0028 0.001 0.0005 0.0004 0.0003 0.0001 0.001 0.0013 0.0035 0.0004 0.0003 0.0004
Cell Periphery 0 0 0 0 0 0 0.0006 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0
Cytoplasm 0.0019 0.0026 0.0032 0.0012 0.0003 0.0016 0.0055 0.0162 0.0058 0.0009 0.0008 0.0023 0.0022 0.017 0.004 0.0007 0.0009 0.0011
Cytoplasmic Foci 0.0262 0.0222 0.0191 0.0593 0.0797 0.0173 0.0253 0.0208 0.014 0.039 0.0713 0.0099 0.0234 0.021 0.0112 0.0226 0.1117 0.0071
Eisosomes 0 0 0 0 0 0.0001 0.0002 0 0.0001 0 0.0001 0.0001 0.0004 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0001 0 0.0001 0.0001 0 0.0002 0.0012 0.0002 0.0003 0.0004 0.0005 0.0003 0.0007 0.0003 0.0005 0.0002 0 0.0002
Endosome 0.0024 0.0018 0.0039 0.0042 0.0044 0.0031 0.0159 0.0021 0.0084 0.0253 0.0129 0.0024 0.0109 0.0042 0.0028 0.0103 0.0064 0.0032
Golgi 0.001 0.0004 0.0007 0.0016 0.0021 0.0008 0.0029 0.0005 0.0012 0.0028 0.0057 0.0005 0.0036 0.0008 0.0025 0.0088 0.0039 0.0009
Lipid Particles 0.008 0.0039 0.0049 0.0193 0.0497 0.0078 0.0243 0.0052 0.0084 0.0585 0.0881 0.0033 0.0196 0.0093 0.0042 0.0169 0.1003 0.0048
Mitochondria 0.0001 0 0.0002 0.0001 0 0.0002 0.0046 0.0001 0.0007 0.0004 0.0013 0.0003 0.0075 0.0003 0.0126 0.005 0.0002 0.0016
None 0.0086 0.0017 0.0114 0.0102 0.0003 0.0116 0.0151 0.0385 0.0105 0.0007 0.002 0.0161 0.0131 0.0541 0.0108 0.0028 0.0081 0.0093
Nuclear Periphery 0.0034 0.0011 0.0008 0.0022 0.0006 0.0036 0.0087 0.0017 0.0041 0.0045 0.0132 0.0061 0.007 0.0076 0.0081 0.0032 0.0016 0.0053
Nucleolus 0.0122 0.0278 0.0278 0.006 0.005 0.0184 0.0278 0.022 0.0338 0.0522 0.008 0.0323 0.0356 0.0558 0.0253 0.0288 0.0304 0.0369
Nucleus 0.0195 0.0485 0.0134 0.0166 0.001 0.0137 0.0351 0.0765 0.0291 0.0328 0.0027 0.0399 0.0397 0.1523 0.0687 0.0461 0.0045 0.043
Peroxisomes 0.0078 0.0009 0.002 0.0128 0.0148 0.002 0.0113 0.0057 0.0031 0.0059 0.0294 0.0023 0.0057 0.008 0.0119 0.0092 0.0225 0.0011
Punctate Nuclear 0.9065 0.8878 0.9109 0.8656 0.8416 0.9172 0.8054 0.8075 0.8707 0.7738 0.7557 0.879 0.8063 0.6655 0.8178 0.8378 0.7076 0.8779
Vacuole 0.0001 0.0002 0.0004 0 0.0001 0.0002 0.0018 0.0004 0.0043 0.0007 0.0006 0.0019 0.0039 0.0008 0.0007 0.0004 0.0002 0.0008
Vacuole Periphery 0 0 0.0001 0 0 0.0002 0.0012 0.0001 0.0007 0.0003 0.0006 0.0006 0.0016 0.0002 0.0007 0.0002 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.9584 7.9976 13.8474 13.2175 9.0219 11.9221 13.4774 12.1284 16.6187 15.9076
Translational Efficiency 1.8387 1.1967 1.0674 1.1194 1.0998 1.2929 1.2183 1.4308 1.214 1.0514

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Outer kinetochore protein, needed for accurate chromosome segregation; component of kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as platform for kinetochore assembly; required for spindle assembly checkpoint; minimizes potentially deleterious centromere-proximal crossovers by preventing meiotic DNA break formation proximal to centromere; homolog of human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2
Localization
Cell Percentages spindle pole (47%), nucleolus (8%), mixed (33%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ctf19

Ctf19


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ctf19-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available