Standard name | |
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Human Ortholog | |
Description | Single-stranded DNA endonuclease (with Rad10p); cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 2 | 3 | 2 | 3 | 6 | 3 | 12 | 4 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 3 | 5 | 9 | 17 | 23 | 14 | 14 | 9 | 23 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 4 | 12 | 19 | 21 | 26 | 27 | 28 | 20 | 18 | 20 | 21 | 9 | 17 | 18 | 21 | 21 | 12 | 1 | 0 | 2 | 1 | 0 | 2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 7 | 4 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 2 | 3 | 1 | 2 | 0 | 0 | 0 | 3 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 46 | 145 | 149 | 158 | 259 | 341 | 377 | 251 | 303 | 245 | 314 | 9 | 3 | 26 | 11 | 6 | 11 | 0 | 6 | 2 | 0 | 0 | 4 |
Nucleus | 146 | 211 | 207 | 164 | 222 | 234 | 332 | 357 | 197 | 272 | 198 | 245 | 62 | 97 | 94 | 156 | 147 | 105 | 148 | 218 | 219 | 61 | 120 | 102 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 2 | 2 | 1 | 4 | 0 | 4 | 6 | 7 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 2 | 0 | 1 | 4 | 1 | 3 | 9 | 4 | 3 | 3 | 8 | 1 | 1 | 2 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 3 | 9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 1 | 2 | 1 | 5 | 9 | 1 | 4 | 0 | 3 | 0 | 1 | 0 | 3 | 1 | 1 | 2 | 1 | 6 | 1 | 0 | 0 |
Vac/Vac Membrane | 7 | 13 | 4 | 1 | 8 | 3 | 2 | 3 | 2 | 0 | 3 | 2 | 1 | 5 | 11 | 25 | 25 | 18 | 6 | 23 | 44 | 5 | 20 | 53 |
Unique Cell Count | 158 | 240 | 268 | 223 | 311 | 347 | 476 | 527 | 311 | 405 | 310 | 376 | 75 | 120 | 131 | 205 | 191 | 136 | 169 | 261 | 279 | 70 | 148 | 182 |
Labelled Cell Count | 161 | 277 | 371 | 342 | 426 | 539 | 732 | 817 | 492 | 631 | 488 | 635 | 82 | 126 | 152 | 235 | 213 | 156 | 169 | 261 | 279 | 70 | 148 | 182 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.9 | 4.7 | 3.5 | 3.5 | 3.6 | 3.5 | 3.2 | 3.3 | 3.0 | 3.0 | 3.1 | 2.8 | 4.9 | 5.1 | 4.3 | 6.4 | 6.8 | 7.3 | 4.8 | 5.5 | 6.0 |
Std Deviation (1e-4) | 1.0 | 0.9 | 0.6 | 1.1 | 1.0 | 1.6 | 0.9 | 0.9 | 1.2 | 1.0 | 1.2 | 1.0 | 1.0 | 0.9 | 0.9 | 1.5 | 1.8 | 2.7 | 0.7 | 1.6 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.03 | -0.01 | -0.15 | -0.11 | -0.25 | -0.26 | -0.21 | -0.35 | 0.47 | 0.53 | 0.28 | 0.85 | 0.94 | 1.05 | 0.43 | 0.64 | 0.77 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 4.8 | 5.0 | 4.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0533 | 1.5909 | 1.4033 | 1.0977 | 1.1303 | 1.6071 | 1.5369 | 2.7322 | 2.2105 | 1.2903 | 1.5582 | 2.1541 | 2.9531 | 3.3826 | 3.2057 | 3.5345 | 2.4563 | 3.1055 |
Actin | 0.0397 | 0 | 0.0069 | 0.0037 | 0.0123 | 0.0098 | 0.0262 | 0.0003 | 0.0054 | 0 | 0.0024 | 0.0001 | 0.0095 | 0 | 0.0009 | 0.021 | 0.0005 | 0.0045 |
Bud | 0.0006 | 0 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0003 | 0 | 0.0003 |
Bud Neck | 0.0139 | 0.0002 | 0.0004 | 0.003 | 0.0013 | 0.0017 | 0.0008 | 0.0019 | 0.0004 | 0.0002 | 0.0006 | 0.0018 | 0.0013 | 0.0001 | 0.001 | 0.0019 | 0.0005 | 0.002 |
Bud Periphery | 0.0013 | 0 | 0.0001 | 0.0025 | 0.0003 | 0.0004 | 0.0004 | 0.0005 | 0.0001 | 0 | 0.0009 | 0.0001 | 0.0013 | 0 | 0.0003 | 0.0005 | 0 | 0.0007 |
Bud Site | 0.015 | 0.0001 | 0.0002 | 0.0038 | 0.0003 | 0.0002 | 0.0015 | 0.0069 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0011 | 0.0001 | 0.0069 | 0.0021 | 0.0001 | 0.0002 |
Cell Periphery | 0.0009 | 0 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0004 | 0.0003 | 0 | 0.0001 |
Cytoplasm | 0.0059 | 0.0037 | 0.0004 | 0.0004 | 0.0009 | 0.002 | 0.0134 | 0.0037 | 0.0196 | 0.0018 | 0.0037 | 0.0059 | 0.003 | 0.0023 | 0.006 | 0.0019 | 0.0071 | 0.0009 |
Cytoplasmic Foci | 0.009 | 0.0001 | 0.0008 | 0.003 | 0.0022 | 0.0025 | 0.0035 | 0.0003 | 0.0021 | 0 | 0.0039 | 0.0002 | 0.0016 | 0 | 0.0008 | 0.0059 | 0.0001 | 0.0002 |
Eisosomes | 0.0008 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0011 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0049 | 0.0004 | 0.0003 | 0.0001 | 0.0023 | 0.0007 | 0.003 | 0.0002 | 0.0022 | 0 | 0.0021 | 0.0012 | 0.0026 | 0.0004 | 0.0008 | 0.0014 | 0.0004 | 0.0002 |
Endosome | 0.0233 | 0.0013 | 0.0044 | 0.0039 | 0.0115 | 0.0024 | 0.0045 | 0.0005 | 0.0044 | 0 | 0.0371 | 0.0017 | 0.009 | 0.0003 | 0.0013 | 0.0476 | 0.0006 | 0.0025 |
Golgi | 0.0068 | 0 | 0.0018 | 0.0035 | 0.0077 | 0.0022 | 0.0028 | 0.0002 | 0.0009 | 0 | 0.0098 | 0 | 0.0034 | 0 | 0.0002 | 0.0119 | 0 | 0.0013 |
Lipid Particles | 0.0268 | 0 | 0.0024 | 0.0031 | 0.0149 | 0.0005 | 0.0025 | 0.0002 | 0.0008 | 0 | 0.004 | 0 | 0.0025 | 0 | 0.0003 | 0.0122 | 0 | 0.0016 |
Mitochondria | 0.0076 | 0.0002 | 0.0014 | 0.0101 | 0.0394 | 0.0026 | 0.0026 | 0.0006 | 0.0006 | 0.0002 | 0.0073 | 0.0008 | 0.0059 | 0.0001 | 0.0005 | 0.0055 | 0.0003 | 0.0063 |
None | 0.0109 | 0.0003 | 0.0001 | 0.0001 | 0.0018 | 0.0004 | 0.0609 | 0.0152 | 0.0408 | 0.1651 | 0.0007 | 0.0179 | 0.0144 | 0.0002 | 0.0046 | 0.0019 | 0.0098 | 0.0191 |
Nuclear Periphery | 0.0467 | 0.0214 | 0.0024 | 0.0009 | 0.0191 | 0.0042 | 0.0272 | 0.0081 | 0.0156 | 0.0028 | 0.0145 | 0.0033 | 0.0108 | 0.0084 | 0.0098 | 0.0028 | 0.0141 | 0.001 |
Nucleolus | 0.008 | 0.0059 | 0.0049 | 0.0094 | 0.0077 | 0.0122 | 0.0066 | 0.0055 | 0.0049 | 0.005 | 0.0027 | 0.0134 | 0.0093 | 0.0022 | 0.0072 | 0.0094 | 0.0062 | 0.0091 |
Nucleus | 0.7262 | 0.9539 | 0.9709 | 0.9394 | 0.8694 | 0.9522 | 0.8138 | 0.9521 | 0.8969 | 0.7934 | 0.8994 | 0.9352 | 0.9092 | 0.9832 | 0.9523 | 0.8411 | 0.9505 | 0.9448 |
Peroxisomes | 0.0095 | 0 | 0.0007 | 0.0058 | 0.0022 | 0.0001 | 0.0061 | 0.0002 | 0.0002 | 0 | 0.0013 | 0 | 0.0006 | 0 | 0.0004 | 0.0213 | 0 | 0.0001 |
Punctate Nuclear | 0.0346 | 0.0109 | 0.0013 | 0.0026 | 0.0042 | 0.0043 | 0.0221 | 0.0019 | 0.0039 | 0.0313 | 0.0051 | 0.0159 | 0.0112 | 0.0018 | 0.0034 | 0.003 | 0.0092 | 0.0043 |
Vacuole | 0.004 | 0.0011 | 0.0003 | 0.0028 | 0.0008 | 0.0008 | 0.0006 | 0.0008 | 0.0006 | 0.0001 | 0.0018 | 0.0017 | 0.0016 | 0.0006 | 0.0025 | 0.0051 | 0.0003 | 0.0004 |
Vacuole Periphery | 0.0038 | 0.0003 | 0.0002 | 0.0006 | 0.0011 | 0.0005 | 0.0007 | 0.0001 | 0.0004 | 0 | 0.0014 | 0.0005 | 0.001 | 0.0001 | 0.0002 | 0.002 | 0.0001 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.6987 | 11.9452 | 18.8357 | 18.8478 | 18.8409 | 15.0244 | 18.4941 | 18.8508 | 21.0628 | 23.0174 |
Translational Efficiency | 0.3666 | 0.39 | 0.2835 | 0.3045 | 0.2671 | 0.3672 | 0.2483 | 0.252 | 0.2353 | 0.1985 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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