Standard name | |
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Human Ortholog | |
Description | Mitochondrial isoleucyl-tRNA synthetase; null mutant is deficient in respiratory growth; human homolog IARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 2 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 4 | 2 | 8 | 4 | 0 | 4 | 7 |
Bud | 2 | 12 | 23 | 44 | 28 | 28 | 34 | 53 | 21 | 19 | 26 | 35 | 1 | 5 | 3 | 2 | 3 | 6 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Neck | 1 | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Bud Site | 0 | 2 | 5 | 5 | 1 | 9 | 11 | 18 | 3 | 6 | 8 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 25 | 8 | 9 | 2 | 4 | 3 | 4 | 4 | 1 | 4 | 5 | 5 | 4 | 3 | 11 | 8 | 8 | 0 | 1 | 0 | 0 | 0 | 1 |
Cytoplasm | 4 | 18 | 22 | 22 | 21 | 24 | 35 | 50 | 18 | 19 | 25 | 23 | 4 | 4 | 9 | 16 | 19 | 15 | 1 | 2 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 3 | 6 | 6 | 1 | 1 | 3 | 4 | 4 | 3 | 0 | 7 | 0 | 2 | 5 | 3 | 9 | 9 | 0 | 0 | 2 | 0 | 1 | 1 | 0 |
Endosome | 43 | 18 | 0 | 1 | 9 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 9 | 16 | 4 | 16 | 22 | 17 | 30 | 36 | 35 | 5 | 8 | 7 |
Golgi | 24 | 8 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 9 | 6 | 39 | 50 | 30 | 13 | 29 | 4 | 4 | 15 | 16 |
Mitochondria | 242 | 492 | 389 | 426 | 407 | 465 | 690 | 737 | 379 | 420 | 345 | 417 | 118 | 90 | 129 | 15 | 17 | 13 | 286 | 441 | 328 | 64 | 129 | 97 |
Nucleus | 6 | 1 | 2 | 3 | 4 | 7 | 14 | 16 | 3 | 3 | 3 | 3 | 0 | 1 | 0 | 0 | 4 | 3 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 17 | 14 | 2 | 0 | 2 | 13 | 19 | 4 | 8 | 7 | 7 | 2 | 2 | 2 | 2 | 2 | 1 | 2 | 2 | 4 | 4 | 1 | 2 | 2 |
Nucleolus | 6 | 4 | 1 | 1 | 3 | 6 | 4 | 4 | 1 | 2 | 3 | 4 | 1 | 0 | 2 | 3 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 |
Peroxisomes | 11 | 23 | 7 | 4 | 13 | 1 | 3 | 7 | 4 | 2 | 2 | 4 | 4 | 10 | 1 | 16 | 1 | 9 | 23 | 18 | 21 | 3 | 16 | 12 |
SpindlePole | 15 | 9 | 16 | 19 | 26 | 20 | 35 | 50 | 11 | 11 | 14 | 24 | 0 | 4 | 0 | 11 | 21 | 4 | 0 | 7 | 3 | 0 | 1 | 0 |
Vac/Vac Membrane | 51 | 100 | 19 | 6 | 28 | 18 | 28 | 20 | 13 | 9 | 11 | 13 | 25 | 17 | 26 | 29 | 28 | 16 | 5 | 26 | 4 | 3 | 3 | 4 |
Unique Cell Count | 311 | 559 | 411 | 449 | 446 | 499 | 736 | 795 | 396 | 435 | 371 | 441 | 135 | 126 | 152 | 149 | 148 | 99 | 372 | 589 | 418 | 87 | 187 | 158 |
Labelled Cell Count | 431 | 734 | 505 | 544 | 547 | 600 | 882 | 968 | 468 | 499 | 456 | 537 | 175 | 168 | 189 | 169 | 187 | 128 | 372 | 589 | 418 | 87 | 187 | 158 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 5.1 | 3.8 | 4.2 | 3.9 | 3.7 | 3.4 | 3.4 | 3.0 | 3.0 | 3.1 | 3.0 | 5.8 | 5.8 | 5.0 | 7.6 | 7.9 | 7.6 | 5.2 | 6.6 | 7.2 |
Std Deviation (1e-4) | 0.8 | 1.1 | 0.7 | 3.4 | 1.0 | 1.1 | 0.8 | 0.9 | 0.8 | 1.0 | 1.3 | 3.4 | 1.0 | 1.0 | 0.8 | 1.9 | 1.9 | 2.2 | 0.9 | 2.0 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.16 | 0.05 | -0.04 | -0.14 | -0.15 | -0.33 | -0.33 | -0.28 | -0.31 | 0.62 | 0.63 | 0.42 | 1.01 | 1.07 | 1.02 | 0.47 | 0.81 | 0.93 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.4 | -0.7 | -1.8 | -2.2 | -1.8 | 0.2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 |
Cytoplasm | -0.3 | -0.4 | -0.4 | -0.4 | 0.7 | -0.5 | -0.7 | 0.8 | -0.1 | -1.1 | -1.0 | 0.3 | 2.2 | 3.0 | 3.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8.0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10.5 | 12.2 | 11.2 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.8 | -7.3 | -3.8 | -19.7 | -19.5 | -17.8 |
Nucleus | 0 | 0 | 0 | 2.0 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.8 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 4.3 | 0 |
Vacuole | -2.9 | 1.1 | -0.8 | -0.7 | -2.0 | -1.0 | -2.1 | -1.2 | -1.3 | 5.1 | 3.5 | 4.8 | 5.5 | 5.4 | 4.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3663 | 2.408 | 2.6216 | 2.2797 | 2.2563 | 2.6328 | 4.1682 | 4.4495 | 4.4082 | 4.6338 | 5.136 | 4.3383 | 4.5758 | 4.4453 | 4.8244 | 5.1958 | 4.9478 | 4.8361 |
Actin | 0.0172 | 0.016 | 0.0159 | 0.0137 | 0.0033 | 0.0092 | 0.0146 | 0.0134 | 0.0126 | 0.0077 | 0.0339 | 0.0042 | 0.028 | 0.0256 | 0.0194 | 0.0018 | 0.0099 | 0.0099 |
Bud | 0.0005 | 0.0012 | 0.0019 | 0.0007 | 0.0014 | 0.0008 | 0.001 | 0.0024 | 0.0013 | 0.0017 | 0.0049 | 0.0005 | 0.0006 | 0.0019 | 0.0009 | 0.0033 | 0.0006 | 0.0006 |
Bud Neck | 0.0009 | 0.0007 | 0.0018 | 0.0022 | 0.0003 | 0.0016 | 0.0052 | 0.001 | 0.0011 | 0.0007 | 0.0097 | 0.0008 | 0.0008 | 0.0005 | 0.0011 | 0.0007 | 0.0003 | 0.0005 |
Bud Periphery | 0.0012 | 0.0024 | 0.006 | 0.0015 | 0.0046 | 0.0035 | 0.0019 | 0.0045 | 0.0026 | 0.0134 | 0.0099 | 0.002 | 0.0014 | 0.0022 | 0.0023 | 0.0094 | 0.003 | 0.0017 |
Bud Site | 0.0018 | 0.0102 | 0.0066 | 0.002 | 0.0017 | 0.001 | 0.0049 | 0.0214 | 0.0138 | 0.0006 | 0.007 | 0.0006 | 0.0056 | 0.0096 | 0.0074 | 0.0017 | 0.0008 | 0.0008 |
Cell Periphery | 0.0005 | 0.0026 | 0.0033 | 0.0003 | 0.0008 | 0.0005 | 0.0012 | 0.0009 | 0.0009 | 0.0006 | 0.0012 | 0.0007 | 0.0007 | 0.0004 | 0.0064 | 0.0013 | 0.003 | 0.0006 |
Cytoplasm | 0.003 | 0.0027 | 0.0042 | 0.0003 | 0.0095 | 0.0015 | 0.0123 | 0.012 | 0.0065 | 0.0018 | 0.0153 | 0.0043 | 0.0029 | 0.0027 | 0.0054 | 0.0226 | 0.004 | 0.0034 |
Cytoplasmic Foci | 0.0182 | 0.0315 | 0.0177 | 0.0117 | 0.0156 | 0.0172 | 0.0302 | 0.025 | 0.0242 | 0.0171 | 0.0279 | 0.0239 | 0.0234 | 0.0473 | 0.0333 | 0.0203 | 0.0232 | 0.0233 |
Eisosomes | 0.0004 | 0.0007 | 0.0009 | 0.0002 | 0.0002 | 0.0003 | 0.0005 | 0.0003 | 0.0011 | 0.0003 | 0.0008 | 0.0002 | 0.0012 | 0.0005 | 0.0016 | 0.0002 | 0.0015 | 0.0004 |
Endoplasmic Reticulum | 0.0037 | 0.0006 | 0.0021 | 0.0006 | 0.0022 | 0.001 | 0.003 | 0.001 | 0.0011 | 0.001 | 0.0028 | 0.0014 | 0.0006 | 0.0003 | 0.0011 | 0.0012 | 0.0004 | 0.0024 |
Endosome | 0.0643 | 0.0576 | 0.0524 | 0.0222 | 0.0569 | 0.0474 | 0.0854 | 0.0755 | 0.0464 | 0.0417 | 0.1085 | 0.0556 | 0.0507 | 0.0489 | 0.0467 | 0.083 | 0.0258 | 0.0429 |
Golgi | 0.0457 | 0.0732 | 0.0479 | 0.024 | 0.0597 | 0.0385 | 0.0519 | 0.067 | 0.0337 | 0.0461 | 0.0809 | 0.0538 | 0.0813 | 0.1056 | 0.0417 | 0.0336 | 0.0669 | 0.0719 |
Lipid Particles | 0.0379 | 0.0354 | 0.0174 | 0.0167 | 0.0034 | 0.0166 | 0.0317 | 0.0121 | 0.0057 | 0.0039 | 0.004 | 0.0111 | 0.0204 | 0.0128 | 0.0067 | 0.0022 | 0.0068 | 0.0141 |
Mitochondria | 0.7458 | 0.7183 | 0.7832 | 0.8809 | 0.7923 | 0.8161 | 0.6882 | 0.7231 | 0.7984 | 0.8362 | 0.6492 | 0.8056 | 0.7241 | 0.6616 | 0.7891 | 0.7842 | 0.7886 | 0.785 |
None | 0.0025 | 0.0014 | 0.001 | 0.0003 | 0.0011 | 0.0011 | 0.0227 | 0.0012 | 0.0136 | 0.002 | 0.0029 | 0.003 | 0.0063 | 0.0006 | 0.0045 | 0.0015 | 0.0032 | 0.0023 |
Nuclear Periphery | 0.0064 | 0.0002 | 0.0018 | 0.0008 | 0.0025 | 0.0057 | 0.0067 | 0.0003 | 0.0003 | 0.0028 | 0.0084 | 0.0018 | 0.0001 | 0.0001 | 0.0006 | 0.0004 | 0.0001 | 0.0004 |
Nucleolus | 0.0005 | 0.0003 | 0.0006 | 0.0005 | 0.0003 | 0.0007 | 0.0008 | 0.0008 | 0.0007 | 0.0001 | 0.0014 | 0.0003 | 0.0004 | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.0001 |
Nucleus | 0.0015 | 0.0002 | 0.0008 | 0.0003 | 0.0032 | 0.0008 | 0.0015 | 0.0006 | 0.0007 | 0.0004 | 0.005 | 0.0018 | 0.0002 | 0.0001 | 0.0004 | 0.0004 | 0.0001 | 0.0001 |
Peroxisomes | 0.0327 | 0.0387 | 0.0247 | 0.0162 | 0.033 | 0.0282 | 0.0215 | 0.0191 | 0.0261 | 0.0143 | 0.0064 | 0.0199 | 0.0469 | 0.0761 | 0.0259 | 0.0094 | 0.0592 | 0.0362 |
Punctate Nuclear | 0.0009 | 0.0002 | 0.0007 | 0.001 | 0.0006 | 0.0007 | 0.0016 | 0.0002 | 0.0002 | 0.0001 | 0.0027 | 0.0013 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Vacuole | 0.0075 | 0.0029 | 0.005 | 0.0021 | 0.0037 | 0.0033 | 0.007 | 0.0123 | 0.0062 | 0.0028 | 0.0102 | 0.0038 | 0.0021 | 0.0014 | 0.0027 | 0.0127 | 0.0013 | 0.0014 |
Vacuole Periphery | 0.0068 | 0.003 | 0.0042 | 0.0019 | 0.0037 | 0.0044 | 0.0061 | 0.0059 | 0.0028 | 0.0047 | 0.0071 | 0.0035 | 0.002 | 0.0016 | 0.002 | 0.0096 | 0.0011 | 0.0019 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.6006 | 3.9337 | 8.0272 | 9.5375 | 8.3203 | 9.8955 | 9.4462 | 12.863 | 10.0424 | 10.7616 |
Translational Efficiency | 0.537 | 0.8306 | 0.3474 | 0.3126 | 0.3335 | 0.3446 | 0.4433 | 0.3469 | 0.403 | 0.3589 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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