Standard name
Human Ortholog
Description Mitochondrial isoleucyl-tRNA synthetase; null mutant is deficient in respiratory growth; human homolog IARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0.06 0.1 0.06 0.06 0.05 0.07 0.05 0 0.07 0.08 0 0 0 0 0 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.08 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.05 0.05 0.05 0.05 0.06 0.05 0 0.07 0.05 0 0 0.06 0.11 0.13 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Endosome 0.14 0 0 0 0 0 0 0 0 0 0 0 0.07 0.13 0 0.11 0.15 0.17 0.08 0.06 0.08 0.06 0 0.05
Golgi 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.26 0.34 0.3 0 0.05 0 0.05 0.09 0.11
Mitochondria 0.78 0.88 0.95 0.95 0.91 0.93 0.94 0.93 0.96 0.97 0.93 0.95 0.87 0.71 0.85 0.1 0.11 0.13 0.77 0.75 0.79 0.74 0.69 0.62
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.11 0 0.09 0.06 0 0.05 0 0.09 0.08
SpindlePole 0.05 0 0 0 0.06 0 0.05 0.06 0 0 0 0.05 0 0 0 0.07 0.14 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.18 0.05 0 0.06 0 0 0 0 0 0 0 0.19 0.13 0.17 0.19 0.19 0.16 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 2 2 3 1 0 0 0 0 0 0 0 0 1 1 0 1 4 2 8 4 0 4 7
Bud 2 12 23 44 28 28 34 53 21 19 26 35 1 5 3 2 3 6 0 1 0 0 0 0
Bud Neck 1 0 2 0 1 2 0 0 0 0 1 1 0 0 0 0 2 0 0 0 2 0 0 0
Bud Site 0 2 5 5 1 9 11 18 3 6 8 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 25 8 9 2 4 3 4 4 1 4 5 5 4 3 11 8 8 0 1 0 0 0 1
Cytoplasm 4 18 22 22 21 24 35 50 18 19 25 23 4 4 9 16 19 15 1 2 0 0 0 0
Endoplasmic Reticulum 3 6 6 1 1 3 4 4 3 0 7 0 2 5 3 9 9 0 0 2 0 1 1 0
Endosome 43 18 0 1 9 0 2 1 0 0 0 0 9 16 4 16 22 17 30 36 35 5 8 7
Golgi 24 8 1 0 0 0 0 0 0 0 0 0 4 9 6 39 50 30 13 29 4 4 15 16
Mitochondria 242 492 389 426 407 465 690 737 379 420 345 417 118 90 129 15 17 13 286 441 328 64 129 97
Nucleus 6 1 2 3 4 7 14 16 3 3 3 3 0 1 0 0 4 3 0 1 0 0 0 0
Nuclear Periphery 17 14 2 0 2 13 19 4 8 7 7 2 2 2 2 2 1 2 2 4 4 1 2 2
Nucleolus 6 4 1 1 3 6 4 4 1 2 3 4 1 0 2 3 1 1 1 1 0 0 0 1
Peroxisomes 11 23 7 4 13 1 3 7 4 2 2 4 4 10 1 16 1 9 23 18 21 3 16 12
SpindlePole 15 9 16 19 26 20 35 50 11 11 14 24 0 4 0 11 21 4 0 7 3 0 1 0
Vac/Vac Membrane 51 100 19 6 28 18 28 20 13 9 11 13 25 17 26 29 28 16 5 26 4 3 3 4
Unique Cell Count 311 559 411 449 446 499 736 795 396 435 371 441 135 126 152 149 148 99 372 589 418 87 187 158
Labelled Cell Count 431 734 505 544 547 600 882 968 468 499 456 537 175 168 189 169 187 128 372 589 418 87 187 158


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 5.1 3.8 4.2 3.9 3.7 3.4 3.4 3.0 3.0 3.1 3.0 5.8 5.8 5.0 7.6 7.9 7.6 5.2 6.6 7.2
Std Deviation (1e-4) 0.8 1.1 0.7 3.4 1.0 1.1 0.8 0.9 0.8 1.0 1.3 3.4 1.0 1.0 0.8 1.9 1.9 2.2 0.9 2.0 1.8
Intensity Change (Log2) – – – 0.16 0.05 -0.04 -0.14 -0.15 -0.33 -0.33 -0.28 -0.31 0.62 0.63 0.42 1.01 1.07 1.02 0.47 0.81 0.93


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 -2.4 -0.7 -1.8 -2.2 -1.8 0.2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 3.1 0 0
Cytoplasm -0.3 -0.4 -0.4 -0.4 0.7 -0.5 -0.7 0.8 -0.1 -1.1 -1.0 0.3 2.2 3.0 3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 8.0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 10.5 12.2 11.2
Mitochondria 0 0 0 0 0 0 0 0 0 -2.8 -7.3 -3.8 -19.7 -19.5 -17.8
Nucleus 0 0 0 2.0 2.1 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 4.8 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1.7 4.3 0
Vacuole -2.9 1.1 -0.8 -0.7 -2.0 -1.0 -2.1 -1.2 -1.3 5.1 3.5 4.8 5.5 5.4 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3663 2.408 2.6216 2.2797 2.2563 2.6328 4.1682 4.4495 4.4082 4.6338 5.136 4.3383 4.5758 4.4453 4.8244 5.1958 4.9478 4.8361
Actin 0.0172 0.016 0.0159 0.0137 0.0033 0.0092 0.0146 0.0134 0.0126 0.0077 0.0339 0.0042 0.028 0.0256 0.0194 0.0018 0.0099 0.0099
Bud 0.0005 0.0012 0.0019 0.0007 0.0014 0.0008 0.001 0.0024 0.0013 0.0017 0.0049 0.0005 0.0006 0.0019 0.0009 0.0033 0.0006 0.0006
Bud Neck 0.0009 0.0007 0.0018 0.0022 0.0003 0.0016 0.0052 0.001 0.0011 0.0007 0.0097 0.0008 0.0008 0.0005 0.0011 0.0007 0.0003 0.0005
Bud Periphery 0.0012 0.0024 0.006 0.0015 0.0046 0.0035 0.0019 0.0045 0.0026 0.0134 0.0099 0.002 0.0014 0.0022 0.0023 0.0094 0.003 0.0017
Bud Site 0.0018 0.0102 0.0066 0.002 0.0017 0.001 0.0049 0.0214 0.0138 0.0006 0.007 0.0006 0.0056 0.0096 0.0074 0.0017 0.0008 0.0008
Cell Periphery 0.0005 0.0026 0.0033 0.0003 0.0008 0.0005 0.0012 0.0009 0.0009 0.0006 0.0012 0.0007 0.0007 0.0004 0.0064 0.0013 0.003 0.0006
Cytoplasm 0.003 0.0027 0.0042 0.0003 0.0095 0.0015 0.0123 0.012 0.0065 0.0018 0.0153 0.0043 0.0029 0.0027 0.0054 0.0226 0.004 0.0034
Cytoplasmic Foci 0.0182 0.0315 0.0177 0.0117 0.0156 0.0172 0.0302 0.025 0.0242 0.0171 0.0279 0.0239 0.0234 0.0473 0.0333 0.0203 0.0232 0.0233
Eisosomes 0.0004 0.0007 0.0009 0.0002 0.0002 0.0003 0.0005 0.0003 0.0011 0.0003 0.0008 0.0002 0.0012 0.0005 0.0016 0.0002 0.0015 0.0004
Endoplasmic Reticulum 0.0037 0.0006 0.0021 0.0006 0.0022 0.001 0.003 0.001 0.0011 0.001 0.0028 0.0014 0.0006 0.0003 0.0011 0.0012 0.0004 0.0024
Endosome 0.0643 0.0576 0.0524 0.0222 0.0569 0.0474 0.0854 0.0755 0.0464 0.0417 0.1085 0.0556 0.0507 0.0489 0.0467 0.083 0.0258 0.0429
Golgi 0.0457 0.0732 0.0479 0.024 0.0597 0.0385 0.0519 0.067 0.0337 0.0461 0.0809 0.0538 0.0813 0.1056 0.0417 0.0336 0.0669 0.0719
Lipid Particles 0.0379 0.0354 0.0174 0.0167 0.0034 0.0166 0.0317 0.0121 0.0057 0.0039 0.004 0.0111 0.0204 0.0128 0.0067 0.0022 0.0068 0.0141
Mitochondria 0.7458 0.7183 0.7832 0.8809 0.7923 0.8161 0.6882 0.7231 0.7984 0.8362 0.6492 0.8056 0.7241 0.6616 0.7891 0.7842 0.7886 0.785
None 0.0025 0.0014 0.001 0.0003 0.0011 0.0011 0.0227 0.0012 0.0136 0.002 0.0029 0.003 0.0063 0.0006 0.0045 0.0015 0.0032 0.0023
Nuclear Periphery 0.0064 0.0002 0.0018 0.0008 0.0025 0.0057 0.0067 0.0003 0.0003 0.0028 0.0084 0.0018 0.0001 0.0001 0.0006 0.0004 0.0001 0.0004
Nucleolus 0.0005 0.0003 0.0006 0.0005 0.0003 0.0007 0.0008 0.0008 0.0007 0.0001 0.0014 0.0003 0.0004 0.0002 0.0005 0.0003 0.0001 0.0001
Nucleus 0.0015 0.0002 0.0008 0.0003 0.0032 0.0008 0.0015 0.0006 0.0007 0.0004 0.005 0.0018 0.0002 0.0001 0.0004 0.0004 0.0001 0.0001
Peroxisomes 0.0327 0.0387 0.0247 0.0162 0.033 0.0282 0.0215 0.0191 0.0261 0.0143 0.0064 0.0199 0.0469 0.0761 0.0259 0.0094 0.0592 0.0362
Punctate Nuclear 0.0009 0.0002 0.0007 0.001 0.0006 0.0007 0.0016 0.0002 0.0002 0.0001 0.0027 0.0013 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001
Vacuole 0.0075 0.0029 0.005 0.0021 0.0037 0.0033 0.007 0.0123 0.0062 0.0028 0.0102 0.0038 0.0021 0.0014 0.0027 0.0127 0.0013 0.0014
Vacuole Periphery 0.0068 0.003 0.0042 0.0019 0.0037 0.0044 0.0061 0.0059 0.0028 0.0047 0.0071 0.0035 0.002 0.0016 0.002 0.0096 0.0011 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.6006 3.9337 8.0272 9.5375 8.3203 9.8955 9.4462 12.863 10.0424 10.7616
Translational Efficiency 0.537 0.8306 0.3474 0.3126 0.3335 0.3446 0.4433 0.3469 0.403 0.3589

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial isoleucyl-tRNA synthetase; null mutant is deficient in respiratory growth; human homolog IARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant
Localization
Cell Percentages mitochondrion (93%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Ism1

Ism1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ism1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available