Standard name
Human Ortholog
Description Subunit of Golgi mannosyltransferase complex; this complex mediates elongation of the polysaccharide mannan backbone; forms a separate complex with Van1p that is also involved in backbone elongation; this complex also contains Anp1p, Mnn10p, Mnn11p, and Hoc1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0.05 0 0 0 0
Bud 0 0 0.05 0 0.05 0.07 0.05 0.08 – 0 0.08 0.07 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.21 0.12 0.07 0.17 0.06 0.05 0 0.05 – 0 0 0 – – – 0.06 0.09 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Endosome 0.16 0.36 0.28 0.42 0.47 0.17 0.23 0.34 – 0.27 0.08 0.1 – – – 0.34 0.46 0.4 0.42 0.35 0.3 0.07 0.42 0
Golgi 0 0.11 0 0 0 0 0 0 – 0.05 0.06 0.08 – – – 0.31 0.17 0.3 0.29 0.34 0.4 0.42 0.06 1.0
Mitochondria 0.55 0.45 0.58 0.31 0.45 0.74 0.63 0.48 – 0.57 0.84 0.78 – – – 0.32 0.16 0.17 0.1 0.14 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.18 0.18 0.28 0.15 0.26 0.32 – 0.28 0.15 0.17 – – – 0.05 0.13 0.15 0 0.05 0.13 0.08 0.38 0
SpindlePole 0 0.05 0.08 0.08 0.11 0.07 0.11 0.16 – 0.12 0.07 0.07 – – – 0.14 0.17 0.15 0 0 0 0 0.14 0
Vac/Vac Membrane 0.08 0.05 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 3 4 0 1 7 0 0 – 8 6 10 – – – 7 6 3 2 15 10 0 0 0
Bud 0 7 24 3 25 61 52 70 – 35 54 60 – – – 10 11 8 0 1 1 0 0 0
Bud Neck 0 0 0 0 0 0 1 1 – 0 0 0 – – – 0 3 0 0 1 1 0 0 0
Bud Site 0 0 0 0 5 7 6 5 – 6 13 10 – – – 0 0 0 – – – – – –
Cell Periphery 0 1 0 3 3 4 2 3 – 2 3 3 – – – 1 1 2 0 0 0 0 0 0
Cytoplasm 8 25 32 42 27 41 41 48 – 29 16 31 – – – 29 40 24 2 1 6 0 0 0
Endoplasmic Reticulum 1 2 1 2 0 6 2 2 – 2 1 11 – – – 11 8 10 0 1 3 0 0 0
Endosome 6 77 125 102 223 137 224 313 – 220 57 89 – – – 161 210 177 34 112 154 0 0 0
Golgi 1 23 7 11 4 8 10 18 – 43 45 73 – – – 146 77 133 24 109 208 0 0 0
Mitochondria 21 95 253 76 213 604 602 445 – 466 594 689 – – – 149 73 74 8 44 46 0 0 0
Nucleus 1 0 3 1 0 3 5 4 – 1 0 2 – – – 1 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 1 1 0 0 0 0 – 1 1 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 3 0 5 1 5 16 24 12 – 12 9 15 – – – 1 4 3 0 0 0 0 0 0
Peroxisomes 0 9 80 43 135 124 247 291 – 227 109 151 – – – 26 60 67 1 14 66 0 0 0
SpindlePole 1 10 33 19 53 56 108 152 – 95 47 61 – – – 68 79 65 2 4 6 0 0 0
Vac/Vac Membrane 3 11 5 1 7 7 17 18 – 18 8 12 – – – 12 15 12 2 8 2 0 0 0
Unique Cell Count 38 212 439 245 478 816 959 923 814 710 889 473 458 445 83 323 516 2 1 1
Labelled Cell Count 47 263 573 305 701 1081 1341 1382 1165 963 1217 622 588 579 83 323 516 2 1 1


Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 6.6 5.7 6.1 5.7 5.2 5.2 5.8 – 5.8 5.6 5.8 – – – 6.9 6.8 7.1 – – –
Std Deviation (1e-4) 1.6 1.4 1.6 1.8 1.7 1.3 1.7 1.8 – 1.4 1.3 1.5 – – – 0.8 1.1 0.9 – – –
Intensity Change (Log2) – – – 0.1 0.01 -0.11 -0.12 0.03 – 0.03 -0.01 0.04 – – – 0.28 0.26 0.33 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 – – – 0.8 0 0
Bud -2.7 -0.2 1.4 0 1.4 – -0.9 1.4 0.9 – – – -2.7 -2.4 -2.9
Bud Neck 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Cytoplasm 4.0 -1.0 -1.6 -2.4 -1.5 – -2.9 -4.1 -3.1 – – – -0.7 0.8 -1.2
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 – – – 2.8 0 2.7
Endosome 3.5 5.7 -4.9 -2.1 2.0 – -0.5 -9.2 -8.6 – – – 1.8 5.4 3.5
Golgi 2.3 -1.1 -1.0 -0.9 0.5 – 3.2 3.8 4.8 – – – 11.8 7.8 11.5
Mitochondria -6.7 -4.0 5.9 1.8 -3.3 – -0.1 9.7 7.5 – – – -7.9 -13.0 -12.6
Nucleus 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Nucleolus 0 0 1.1 1.7 0.3 – 0.5 0.2 0.8 – – – 0 0 0
Peroxisomes -0.2 3.6 -1.4 3.1 5.2 – 3.8 -1.3 -0.6 – – – -6.0 -2.1 -1.3
SpindlePole 0.1 1.9 -0.4 2.2 4.5 – 2.3 -0.6 -0.4 – – – 3.3 4.4 3.4
Vacuole 0 0.4 0 0.9 1.1 – 1.3 0 0.3 – – – 1.6 2.2 1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – – – – –
Bud Periphery – – – – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – – – – –
Cytoplasmic Foci – – – – – – – – – – – – – – – – – –
Eisosomes – – – – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – – – – –
Lipid Particles – – – – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – – – – –
None – – – – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – – – – –
Punctate Nuclear – – – – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – – – – –
Vacuole Periphery – – – – – – – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 99.3807 54.7987 76.3971 72.1371 89.7143 86.3298 69.4685 77.6249 61.223 90.3639
Translational Efficiency 1.0299 1.2556 0.8091 0.8669 0.732 0.97 0.9247 0.6684 0.8978 0.7037

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization –
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of Golgi mannosyltransferase complex; this complex mediates elongation of the polysaccharide mannan backbone; forms a separate complex with Van1p that is also involved in backbone elongation; this complex also contains Anp1p, Mnn10p, Mnn11p, and Hoc1p
Localization
Cell Percentages Golgi (25%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mnn9

Mnn9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mnn9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available