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Basic protein informationORFYPL050C
Common nameMNN9
Alias
DescriptionSubunit of Golgi mannosyltransferase complex also containing Anp1p, Mnn10p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; forms a separate complex with Van1p that is also involved in backbone elongation
Micrographs
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Wild type 1 (WT1)
Wild type 2 (WT2)
Wild type 3 (WT3)

ORF-GFP <-> cytosolic RFP <-> ORF-GFP/cytosolic RFP overlay        
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Sub-cellular localization
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ensLOCDeepLoc
LocalizationWT1WT2WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700HU80HU120HU160rpd3Δ_1rpd3Δ_2rpd3Δ_3WT1WT2WT3AF100AF140AF180
Cort. Patches.050000000-000---0000.050000
Bud00.050.05.07.05.08-0.08.07---000000000
Budneck00000000-000---000000000
Budsite00000000-000---000000000
Cell Periphery00000000-000---000000000
Cytoplasm.21.12.07.17.06.050.05-000---.06.09.05000000
Endosome.16.36.28.42.47.17.23.34-.27.08.10---.34.46.40.42.35.30.07.420
ER00000000-000---000000000
Golgi0.11000000-.05.06.08---.31.17.30.29.34.40.42.061.00
Mito.55.45.58.31.45.74.63.48-.57.84.78---.32.16.17.10.14.09000
Nuc Periphery00000000-000---000000000
Nucleus00000000-000---000000000
Nucleolus.080000000-000---000000000
Peroxisome00.18.18.28.15.26.32-.28.15.17---.05.13.150.05.13.08.380
SpindlePole0.05.08.08.11.07.11.16-.12.07.07---.14.17.150000.140
Vac/Vac Memb.08.05000000-000---000000000
TotalCells38212439245478816959923-814710889---47345844583323516211

Cell count <-> LOC-score        Download localization matrix
YGFP assignment in Huh et al. (2003) GOLGI
Protein Abundance
HELP on interpreting this table
ScreenWT1WT2WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700HU80HU120HU160rpd3Δ_1rpd3Δ_2rpd3Δ_3AF100AF140AF180
Mean GFP Intensity Ig(x 1e-4)5.76.65.76.15.75.25.25.8-5.85.65.8---6.96.87.1---
Std Deviation (x 1e-4)1.61.41.61.81.71.31.71.8-1.41.31.5---0.81.10.9---
Abundance Change δPL (Log2)nanana0.10.0-0.1-0.10.0-0.0-0.00.0---0.30.30.3---

Plot time-course intensity of:   rapamycin
Sub-cellular localization change
HELP on interpreting this table
Localization/ScreenRAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700HU80HU120HU160rpd3Δ_1rpd3Δ_2rpd3Δ_3
Cort. Patches0000000000000.800
Bud-2.7-0.21.401.40-0.91.40.9000-2.7-2.4-2.9
Budneck000000000000000
Budsite000000000000000
Cell Periphery000000000000000
Cytoplasm4.0-1.0-1.6-2.4-1.50-2.9-4.1-3.1000-0.70.8-1.2
Endosome3.55.7-4.9-2.12.00-0.5-9.2-8.60001.85.43.5
ER0000000000002.802.7
Golgi2.3-1.1-1.0-0.90.503.23.84.800011.87.811.5
Mito-6.7-4.05.91.8-3.30-0.19.77.5000-7.9-13.0-12.6
Nuc Periphery000000000000000
Nucleus000000000000000
Nucleolus001.11.70.300.50.20.8000000
Peroxisome-0.23.6-1.43.15.203.8-1.3-0.6000-6.0-2.1-1.3
SpindlePole0.11.9-0.42.24.502.3-0.6-0.40003.34.43.4
Vac/Vac Memb00.400.91.101.300.30001.62.21.7

Download z-score matrix



Copyright © Moffat Lab and Andrews lab at CCBR, University of Toronto, 2014