Standard name
Human Ortholog
Description ARF-like small GTPase of the RAS superfamily; required for recruitment of Arl1p, a GTPase that regulates membrane traffic, to the Golgi apparatus; NatC-catalyzed N-terminal acetylation regulates Golgi membrane association mediated by interaction with membrane receptor, Sys1p; similar to ADP-ribosylation factor and orthologous to mammalian ARFRP1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.08 0.16 0 0 0 0 – 0 0 0 0 0.18 0.06 0.05 0.07 0.06 0.17 0 0.05 0 0.05 0.05 0.08
Bud 0 0 0 0.07 0 0.05 0 – 0.05 0.08 0.07 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.26 0.29 0.19 0.24 0.29 0.22 – 0.26 0.24 0.2 0.24 0.23 0.35 0.26 0.58 0.64 0.5 0 0.05 0 0.08 0 0.06
Endoplasmic Reticulum 0 0.11 0.1 0 0 0 0 – 0 0 0 0 0.11 0.09 0 0.05 0.06 0 0 0 0 0 0 0
Endosome 0.43 0.21 0.28 0.4 0.39 0.06 0.32 – 0.1 0.1 0.12 0.06 0.34 0.42 0.47 0.24 0.12 0.22 0.36 0.27 0.28 0.29 0.36 0.32
Golgi 0.05 0.06 0.08 0 0 0 0 – 0 0 0 0 0.11 0.05 0 0 0 0.12 0.35 0.23 0.23 0.3 0.3 0.29
Mitochondria 0.38 0.36 0.05 0.26 0.2 0.67 0.29 – 0.65 0.58 0.65 0.66 0.16 0.07 0.14 0.08 0 0 0.11 0.27 0.28 0.16 0.13 0.13
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0 0 0.08 0.14 0 0.15 – 0 0 0.05 0 0 0 0.06 0 0 0 0.07 0 0.06 0 0 0
SpindlePole 0.09 0 0.21 0.15 0.13 0.08 0.22 – 0.1 0.14 0.11 0.1 0 0 0.05 0.08 0.06 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.09 0 0 0.1 0 0.08 – 0 0.05 0 0 0 0 0.06 0 0.11 0.08 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 16 11 18 7 5 2 0 – 1 5 3 9 21 10 11 7 6 13 9 9 5 7 16 20
Bud 6 1 4 13 10 11 15 – 13 27 18 25 1 4 6 0 0 1 0 1 0 0 1 1
Bud Neck 0 0 0 2 0 1 2 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 2 – 4 4 4 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 2 1 0 1 – 1 4 0 1 0 0 3 1 0 1 0 0 0 0 0 0
Cytoplasm 33 35 33 34 54 61 75 – 65 78 48 68 26 62 54 59 67 38 4 8 3 11 11 16
Endoplasmic Reticulum 9 15 11 1 0 5 0 – 0 1 0 12 12 16 8 5 6 1 3 3 1 0 5 4
Endosome 119 29 31 72 89 12 110 – 25 33 28 18 39 74 99 24 12 17 129 50 48 44 108 79
Golgi 15 8 9 3 0 0 0 – 0 0 0 0 13 9 8 4 2 9 128 42 38 44 88 72
Mitochondria 105 49 6 46 45 139 99 – 162 190 156 186 18 13 29 8 1 2 40 49 48 23 40 31
Nucleus 2 0 1 0 0 1 2 – 3 2 1 2 1 1 3 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 0 – 0 0 0 0 0 1 0 0 0 0 1 0 0 1 0 1
Nucleolus 0 0 0 0 0 1 0 – 2 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 14 1 1 14 33 5 53 – 6 14 11 3 3 3 12 0 0 0 24 3 10 6 12 8
SpindlePole 24 3 24 26 29 16 77 – 24 47 26 27 5 5 11 8 6 3 9 2 4 4 6 2
Vac/Vac Membrane 20 12 3 6 22 5 29 – 9 18 7 5 2 2 12 4 11 6 6 10 3 2 3 3
Unique Cell Count 276 135 112 179 228 209 346 249 330 240 283 114 177 211 102 104 76 364 185 172 150 299 251
Labelled Cell Count 364 165 141 227 288 260 465 315 424 302 363 141 200 256 120 111 91 364 185 172 150 299 251


Early Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.4 6.8 6.4 6.7 4.6 5.8 – 5.2 5.3 5.1 5.0 7.2 7.3 7.5 9.0 9.0 9.5 6.0 6.4 6.5
Std Deviation (1e-4) 0.8 1.0 1.1 1.5 1.3 1.8 1.3 – 2.0 1.3 1.6 1.0 1.5 1.5 1.6 1.7 1.6 2.0 1.2 1.6 1.6
Intensity Change (Log2) – – – -0.09 -0.02 -0.55 -0.22 – -0.4 -0.36 -0.41 -0.44 0.08 0.11 0.15 0.41 0.42 0.49 -0.18 -0.07 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -3.6 -4.8 0 0 – -6.2 -6.0 -5.5 -4.6 0.5 -2.9 -3.2 -2.1 -2.4 0.2
Bud 1.3 0 0 0 – 0.7 1.7 1.4 1.8 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.1 -1.1 -0.1 -1.7 – -0.7 -1.2 -2.0 -1.1 -1.1 1.0 -0.7 4.2 5.1 2.9
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 -2.1 0.2 -0.2 -2.2 -1.4 -1.1 0
Endosome 2.2 2.1 0 0.8 – -4.3 -4.6 -3.7 -5.8 1.1 2.4 3.4 -0.7 -3.0 -0.8
Golgi 0 0 0 0 – 0 0 0 0 0.9 -1.0 -1.6 -1.3 -2.0 0.9
Mitochondria 4.4 3.5 0 5.1 – 10.5 9.6 10.5 10.8 2.5 0.7 2.3 0.7 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 2.6 3.9 0 4.1 – 0 0 0 0 0 0 0 0 0 0
SpindlePole -1.5 -2.1 0 0.2 – -3.1 -1.8 -2.7 -3.2 -3.8 -5.1 -4.5 -2.8 -3.3 -3.4
Vacuole 0 2.3 0 2.1 – 0 1.2 0 0 0 0 1.2 0 2.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4448 1.2485 1.4559 0.7654 0.4574 1.3297 2.612 2.9749 2.7999 2.8486 3.0087 3.1387 4.5948 5.1159 4.9109 4.6735 5.2209 4.9312
Actin 0.0719 0.0153 0.0687 0.0032 0.0087 0.0099 0.0795 0.0145 0.0232 0.0016 0.0519 0.0602 0.0843 0.0406 0.0471 0.064 0.1041 0.078
Bud 0.0013 0.0009 0.0019 0.0006 0.0003 0.0003 0.0011 0.0003 0.0049 0.0066 0.0013 0.001 0.0004 0.0005 0.0003 0.0004 0.0002 0.0003
Bud Neck 0.003 0.0005 0.0027 0.0011 0.0008 0.0016 0.0051 0.0006 0.001 0.0022 0.0007 0.0031 0.0019 0.0012 0.0013 0.001 0.0006 0.0024
Bud Periphery 0.0016 0.0007 0.0014 0.0005 0.0003 0.0002 0.0016 0.0002 0.0036 0.0086 0.0015 0.0015 0.0005 0.0002 0.0003 0.0003 0.0001 0.0002
Bud Site 0.0184 0.0023 0.0105 0.0013 0.0005 0.0004 0.0123 0.0032 0.0086 0.0015 0.005 0.0013 0.0029 0.0087 0.0017 0.0012 0.0005 0.0006
Cell Periphery 0.0008 0.0004 0.0004 0.0001 0.0001 0.0001 0.0008 0.0001 0.0003 0.0006 0.0001 0.0003 0.0004 0.0003 0.0003 0.0002 0.0001 0.0001
Cytoplasm 0.1413 0.1726 0.1363 0.0683 0.0723 0.1602 0.1158 0.2429 0.1822 0.2316 0.061 0.1521 0.0812 0.1485 0.0796 0.0555 0.0196 0.0945
Cytoplasmic Foci 0.2339 0.2602 0.1932 0.2856 0.2551 0.2595 0.2493 0.2553 0.2432 0.1473 0.1716 0.2688 0.3203 0.3133 0.2379 0.1916 0.1311 0.2474
Eisosomes 0.0005 0.0003 0.0004 0.0001 0.0001 0.0001 0.0004 0 0.0001 0 0.0003 0.0001 0.0017 0.0001 0.0006 0.0002 0.0003 0.0002
Endoplasmic Reticulum 0.0111 0.008 0.0082 0.002 0.0042 0.0054 0.0109 0.0055 0.0032 0.0038 0.0039 0.0116 0.0247 0.0057 0.0114 0.0124 0.0098 0.012
Endosome 0.2501 0.2678 0.2842 0.3814 0.4808 0.2719 0.2407 0.2835 0.3007 0.4292 0.3465 0.2245 0.1631 0.2266 0.2449 0.3202 0.2704 0.2359
Golgi 0.1003 0.0825 0.1012 0.1062 0.1231 0.1472 0.1151 0.1365 0.146 0.0894 0.09 0.1007 0.1457 0.1764 0.1877 0.2573 0.2746 0.2066
Lipid Particles 0.0359 0.012 0.0168 0.0521 0.0183 0.0192 0.0435 0.0131 0.0117 0.0048 0.0368 0.0208 0.0445 0.0192 0.0233 0.0071 0.0061 0.0151
Mitochondria 0.0158 0.011 0.0135 0.0051 0.0096 0.0198 0.014 0.0038 0.0058 0.0088 0.0096 0.0062 0.0046 0.0053 0.0184 0.006 0.0041 0.0052
None 0.0603 0.135 0.114 0.0446 0.0023 0.0488 0.0228 0.017 0.0268 0.0051 0.0062 0.0283 0.0507 0.0036 0.0986 0.0239 0.1149 0.0258
Nuclear Periphery 0.0081 0.0027 0.0059 0.0011 0.0017 0.0043 0.0165 0.001 0.0025 0.0017 0.0651 0.0227 0.0058 0.0012 0.002 0.0014 0.0026 0.0019
Nucleolus 0.0007 0.0001 0.0004 0.0002 0.0001 0.0006 0.0016 0.0001 0.0003 0.0006 0.0017 0.0106 0.0013 0.0001 0.0007 0 0 0.0003
Nucleus 0.0017 0.0005 0.0025 0.0006 0.0003 0.007 0.0118 0.0004 0.0046 0.0217 0.0312 0.0277 0.0018 0.0004 0.0021 0.0003 0.0002 0.0006
Peroxisomes 0.032 0.0201 0.0269 0.0181 0.0107 0.0349 0.0432 0.0137 0.0236 0.0039 0.0897 0.0476 0.0363 0.0438 0.0352 0.0482 0.0521 0.0639
Punctate Nuclear 0.0032 0.0015 0.0042 0.0041 0.0011 0.0023 0.0067 0.0009 0.002 0.0004 0.0152 0.0042 0.0257 0.0017 0.0029 0.0009 0.0004 0.0067
Vacuole 0.0058 0.0042 0.0046 0.0142 0.0068 0.0044 0.0045 0.005 0.004 0.0237 0.0065 0.0042 0.0013 0.0017 0.0024 0.0044 0.0047 0.0014
Vacuole Periphery 0.0025 0.0014 0.0018 0.0095 0.0028 0.002 0.0026 0.0025 0.0017 0.007 0.004 0.0025 0.0008 0.0008 0.0013 0.0036 0.0035 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.7996 14.87 14.7122 21.1712 19.1707 24.0139 22.9702 35.5167 37.0789 22.2072
Translational Efficiency 0.7319 0.8888 1.1257 0.8815 0.8168 0.7598 0.8077 0.4818 0.492 0.7584

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description ARF-like small GTPase of the RAS superfamily; required for recruitment of Arl1p, a GTPase that regulates membrane traffic, to the Golgi apparatus; NatC-catalyzed N-terminal acetylation regulates Golgi membrane association mediated by interaction with membrane receptor, Sys1p; similar to ADP-ribosylation factor and orthologous to mammalian ARFRP1
Localization
Cell Percentages mitochondrion (12%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Arl3

Arl3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arl3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available