Standard name
Human Ortholog
Description Plasma membrane ATP-binding cassette (ABC) transporter; weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.22 0.31 0.33 0.34 0.23 0.15 0.08 0 – 0 0 0 0.18 0.22 0.13 – – – 0.09 0.09 0 0.09 0.08 0.09
Bud 0.05 0.1 0.06 0.11 0.09 0.15 0.11 0.07 – 0.08 0.06 0.06 0 0.05 0 – – – 0 0 0 0.05 0.08 0.07
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0.05 0 0 0 0.05 0.07
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0.58 0.66 0.66 0.65 0.38 0.24 0.17 0.09 – 0.05 0.05 0 0.64 0.7 0.73 – – – 0.59 0.71 0.76 0.58 0.57 0.53
Cytoplasm 0.36 0.28 0.24 0.3 0.5 0.53 0.6 0.67 – 0.66 0.64 0.6 0.35 0.28 0.29 – – – 0 0 0 0.06 0 0.05
Endoplasmic Reticulum 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0.11 0.06 0.05 0.09 0.09 0.1
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.08 0.08 0.08 0.08 0.27 0.3 0.28 – 0.41 0.49 0.48 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0.06 0 0.06 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 120 140 91 63 62 39 27 16 – 8 5 6 44 47 37 – – – 54 47 12 39 29 35
Bud 29 44 16 21 25 39 37 29 – 30 18 20 6 11 5 – – – 20 14 3 23 28 28
Bud Neck 1 0 0 0 0 0 0 1 – 0 0 1 1 0 1 – – – 29 17 4 20 17 29
Bud Site 2 4 1 0 1 7 2 6 – 2 4 4 0 3 0 – – – – – – – – –
Cell Periphery 309 300 179 122 101 63 58 40 – 19 15 11 156 151 206 – – – 366 371 230 269 213 218
Cytoplasm 192 128 65 57 133 141 206 296 – 239 189 198 84 61 81 – – – 18 13 6 27 15 19
Endoplasmic Reticulum 22 16 13 3 5 1 3 1 – 0 0 1 5 6 11 – – – 66 30 16 40 34 41
Endosome 3 0 1 0 1 1 0 2 – 0 0 0 0 0 0 – – – 3 4 0 0 1 1
Golgi 2 1 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 2 1 0 1 1 2
Mitochondria 17 38 23 15 20 72 102 125 – 148 144 159 4 5 5 – – – 13 7 4 16 12 9
Nucleus 3 3 1 0 1 1 2 3 – 2 0 2 5 1 2 – – – 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 1 2 3 – 2 1 0 0 0 0 – – – 1 0 0 0 0 0
Nucleolus 1 0 0 0 1 0 0 3 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 3 0 4 9 6 3 3 3 – 0 1 2 0 2 0 – – – 0 0 0 0 0 1
SpindlePole 13 10 10 5 2 1 5 10 – 7 5 17 9 8 5 – – – 0 0 0 0 1 2
Vac/Vac Membrane 10 1 3 3 7 3 7 8 – 3 4 1 0 0 3 – – – 38 12 19 17 16 13
Unique Cell Count 537 456 273 188 264 265 341 440 361 295 331 242 216 283 620 524 301 461 377 410
Labelled Cell Count 728 685 407 298 365 372 454 546 460 386 422 314 295 356 620 524 301 461 377 410


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 9.9 8.4 7.6 6.1 4.9 4.3 4.4 – 3.2 3.3 3.1 8.8 9.5 9.8 – – – 8.7 8.8 8.3
Std Deviation (1e-4) 2.4 3.2 2.5 2.4 2.2 2.4 1.7 1.8 – 1.0 1.2 1.2 3.3 2.7 2.8 – – – 6.7 5.5 4.3
Intensity Change (Log2) – – – -0.13 -0.46 -0.76 -0.96 -0.91 – -1.41 -1.36 -1.43 0.08 0.18 0.23 – – – 0.05 0.07 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -2.5 -5.0 0 0 – 0 0 0 -3.9 -2.8 -5.7 – – –
Bud 2.1 1.6 3.4 0 0 – 0 0 0 -1.9 -0.4 -2.5 – – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Cell Periphery -0.1 -6.3 -9.7 0 0 – 0 0 0 -0.3 1.0 1.8 – – –
Cytoplasm 1.6 6.4 7.0 9.1 11.3 – 10.6 9.6 8.9 2.7 1.1 1.3 – – –
Endoplasmic Reticulum -1.8 -1.8 -3.2 0 0 – 0 0 0 -1.7 -1.1 -0.5 – – –
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Mitochondria -0.2 -0.4 5.7 0 0 – 0 0 0 -3.4 -2.9 -3.6 – – –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Peroxisomes 2.1 0 0 0 0 – 0 0 0 0 0 0 – – –
SpindlePole -0.6 -2.3 -2.7 0 0 – 0 0 0 0 0 -1.4 – – –
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0759 0.0042 0.0176 0.0284 0.0001 0.0125 0.0529 0.0208 0.0104 0.0504 0.0204 0.0046 – – – – – –
Bud 0.0034 0.0018 0.0042 0.0038 0.0006 0.0014 0.015 0.0006 0.0022 0.0071 0.0033 0.0021 – – – – – –
Bud Neck 0.0047 0.0021 0.0275 0.0432 0.0024 0.0241 0.0032 0.0037 0.0227 0.0042 0.0068 0.0123 – – – – – –
Bud Periphery 0.0336 0.0073 0.0215 0.0454 0.014 0.0248 0.0237 0.0042 0.0143 0.0332 0.0469 0.0303 – – – – – –
Bud Site 0.0258 0.0821 0.0541 0.0444 0.0011 0.0058 0.0169 0.151 0.0817 0.0575 0.0108 0.0037 – – – – – –
Cell Periphery 0.6536 0.6273 0.6418 0.6276 0.9193 0.7668 0.5076 0.6066 0.5352 0.4859 0.671 0.6194 – – – – – –
Cytoplasm 0.0386 0.2227 0.1563 0.169 0.0336 0.0941 0.1314 0.1675 0.2434 0.1549 0.1151 0.2246 – – – – – –
Cytoplasmic Foci 0.002 0.0069 0.0086 0.0012 0 0.0004 0.0271 0.0004 0.0055 0.0293 0.0373 0.0105 – – – – – –
Eisosomes 0.0094 0.0011 0.0013 0.0027 0.0002 0.0009 0.0022 0.0006 0.0035 0.0008 0.0006 0.0007 – – – – – –
Endoplasmic Reticulum 0.0368 0.0134 0.01 0.0101 0.0125 0.0189 0.0142 0.0083 0.0123 0.0071 0.0077 0.0037 – – – – – –
Endosome 0.0021 0.0006 0.0043 0.0003 0 0.0108 0.014 0 0.0006 0.0033 0.0055 0.0017 – – – – – –
Golgi 0.0032 0.0006 0.0039 0.001 0.0001 0.0009 0.0094 0.0001 0.0006 0.0059 0.0034 0.0017 – – – – – –
Lipid Particles 0.0052 0.0042 0.0031 0.0016 0.0012 0.0056 0.0333 0.0015 0.0029 0.0114 0.0116 0.0018 – – – – – –
Mitochondria 0.0034 0.0006 0.0023 0.0002 0 0.0001 0.0064 0 0.0046 0.002 0.0005 0.0044 – – – – – –
None 0.037 0.0081 0.0039 0.0005 0.0001 0.0003 0.0725 0.0278 0.0423 0.0891 0.005 0.0574 – – – – – –
Nuclear Periphery 0.0117 0.0012 0.0014 0.0009 0.0008 0.0009 0.0057 0.0004 0.0008 0.0005 0.0014 0.0003 – – – – – –
Nucleolus 0.0007 0.0002 0.0004 0.0002 0 0.0001 0.0015 0.0002 0.0013 0.0018 0.0004 0.0039 – – – – – –
Nucleus 0.0277 0.0005 0.001 0.0007 0.0002 0.0003 0.0062 0.0003 0.0034 0.0017 0.0006 0.002 – – – – – –
Peroxisomes 0.0002 0.0004 0.0149 0.0001 0 0 0.0244 0 0.0001 0.0176 0.0239 0.0004 – – – – – –
Punctate Nuclear 0.0003 0.0002 0.0006 0.0001 0 0 0.0075 0 0.0008 0.0037 0.0191 0.0003 – – – – – –
Vacuole 0.0235 0.0145 0.0211 0.0182 0.0135 0.0307 0.0232 0.0057 0.0111 0.0322 0.0086 0.0135 – – – – – –
Vacuole Periphery 0.001 0.0001 0.0004 0.0004 0.0001 0.0004 0.0018 0 0.0003 0.0003 0.0002 0.0007 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 55.827 92.2009 28.7223 24.2255 19.5542 57.3764 84.2097 45.8545 25.7173 16.7478
Translational Efficiency 0.5538 0.6705 0.5419 0.5232 0.4295 0.693 0.6052 0.395 0.3238 0.2165

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Plasma membrane ATP-binding cassette (ABC) transporter; weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity
Localization
Cell Percentages cell periphery (54%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-3

Pdr12

Pdr12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pdr12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available