Standard name | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 2 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 6 | 0 | – | 2 | 4 | 4 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 4 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 6 | 1 | 3 | 1 | 5 | – | 3 | 7 | 4 | 2 | 1 | 1 | 3 | 5 | 2 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 316 | 157 | 268 | 285 | 347 | 286 | 394 | – | 288 | 268 | 189 | 183 | 260 | 449 | 492 | 204 | 158 | 134 | 308 | 155 | 256 | 183 | 431 | 295 |
Endoplasmic Reticulum | 2 | 1 | 1 | 0 | 0 | 1 | 1 | – | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 7 | 5 | 6 | 0 | 0 | 2 | 2 | 3 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 2 | – | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 1 | 0 |
Mitochondria | 2 | 1 | 0 | 1 | 2 | 42 | 15 | – | 70 | 54 | 70 | 66 | 1 | 0 | 1 | 1 | 1 | 2 | 1 | 0 | 2 | 0 | 3 | 3 |
Nucleus | 0 | 2 | 0 | 0 | 1 | 2 | 11 | – | 5 | 8 | 9 | 5 | 0 | 1 | 0 | 5 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 4 | 5 | 6 | 71 | – | 44 | 41 | 31 | 34 | 2 | 5 | 3 | 6 | 4 | 3 | 0 | 5 | 0 | 0 | 1 | 0 |
Unique Cell Count | 321 | 159 | 270 | 288 | 350 | 311 | 455 | 358 | 332 | 265 | 253 | 263 | 457 | 494 | 212 | 161 | 138 | 325 | 169 | 271 | 191 | 459 | 317 | |
Labelled Cell Count | 322 | 161 | 276 | 291 | 358 | 346 | 500 | 413 | 387 | 311 | 294 | 267 | 459 | 500 | 229 | 172 | 155 | 325 | 169 | 271 | 191 | 459 | 317 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.7 | 6.3 | 6.9 | 6.3 | 7.0 | 4.9 | 6.3 | – | 5.7 | 6.0 | 5.6 | 5.9 | 6.6 | 7.2 | 7.6 | 8.0 | 8.1 | 8.5 | 5.9 | 6.4 | 6.9 |
Std Deviation (1e-4) | 0.8 | 1.2 | 1.2 | 1.4 | 1.6 | 1.2 | 1.5 | – | 1.2 | 1.1 | 1.1 | 1.1 | 1.1 | 1.5 | 1.8 | 1.4 | 1.5 | 2.8 | 1.2 | 1.6 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.03 | -0.5 | -0.12 | – | -0.26 | -0.2 | -0.3 | -0.22 | -0.06 | 0.06 | 0.15 | 0.21 | 0.22 | 0.3 | -0.22 | -0.1 | -0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | -0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 |
Cytoplasm | -0.4 | -0.2 | -4.2 | -5.9 | – | -7.3 | -7.2 | -9.2 | -8.9 | -0.5 | -1.1 | 0.6 | -2.3 | -1.1 | -1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 6.3 | 3.0 | – | 7.7 | 6.9 | 9.1 | 9.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 6.6 | – | 5.7 | 5.7 | 5.5 | 6.0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.3471 | 0.0359 | -0.1314 | -0.2634 | 0.2478 | 0.0611 | 1.296 | 1.594 | 1.265 | 1.1553 | 1.2563 | 1.092 | 3.4371 | 4.0643 | 3.6806 | 3.5426 | 3.7923 | 3.5782 |
Actin | 0.0535 | 0.0017 | 0.016 | 0.0229 | 0.01 | 0.0049 | 0.0494 | 0.0014 | 0.0098 | 0.0048 | 0.0446 | 0.0041 | 0.0689 | 0.0001 | 0.0003 | 0.0004 | 0.0078 | 0.0168 |
Bud | 0.0015 | 0.0032 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0047 | 0.0026 | 0.0009 | 0.0019 | 0.0001 | 0.0013 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0005 |
Bud Neck | 0.0021 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0005 | 0.004 | 0.0003 | 0.0002 | 0.0035 | 0.0008 | 0.0004 | 0.0006 | 0 | 0.0001 | 0.074 | 0.0001 | 0.0007 |
Bud Periphery | 0.0016 | 0.0028 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.003 | 0.0022 | 0.002 | 0.0018 | 0.0001 | 0.0014 | 0 | 0 | 0.0001 | 0.0005 | 0.0007 |
Bud Site | 0.0345 | 0.0029 | 0.0007 | 0.0003 | 0.0001 | 0.0003 | 0.0014 | 0.016 | 0.0022 | 0.0067 | 0.0019 | 0.0001 | 0.0047 | 0.0001 | 0.0009 | 0.001 | 0.0001 | 0.0007 |
Cell Periphery | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.1899 | 0.4274 | 0.4602 | 0.2307 | 0.5887 | 0.5856 | 0.51 | 0.643 | 0.6104 | 0.5481 | 0.5245 | 0.7365 | 0.3723 | 0.546 | 0.5122 | 0.639 | 0.5283 | 0.6169 |
Cytoplasmic Foci | 0.0384 | 0.0069 | 0.007 | 0.0198 | 0.012 | 0.0092 | 0.0074 | 0.0047 | 0.0107 | 0.0355 | 0.0498 | 0.0043 | 0.0097 | 0.0016 | 0.0055 | 0.0085 | 0.0073 | 0.0094 |
Eisosomes | 0.0005 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0075 | 0.002 | 0.0031 | 0.0103 | 0.0026 | 0.0026 | 0.0036 | 0.0014 | 0.0028 | 0.0043 | 0.0019 | 0.0012 | 0.0027 | 0.0015 | 0.0017 | 0.002 | 0.0178 | 0.0017 |
Endosome | 0.0712 | 0.0043 | 0.0058 | 0.0125 | 0.0117 | 0.0096 | 0.0113 | 0.0006 | 0.0075 | 0.008 | 0.0055 | 0.0039 | 0.0062 | 0.0003 | 0.0035 | 0.0037 | 0.0353 | 0.0034 |
Golgi | 0.0178 | 0.0003 | 0.005 | 0.0491 | 0.0214 | 0.0076 | 0.0046 | 0.0001 | 0.0016 | 0.0034 | 0.0088 | 0.0043 | 0.0027 | 0.0001 | 0.0016 | 0.0003 | 0.007 | 0.0031 |
Lipid Particles | 0.0332 | 0.006 | 0.0012 | 0.0373 | 0.0609 | 0.0022 | 0.0165 | 0.0002 | 0.0003 | 0.0035 | 0.0268 | 0.0012 | 0.0015 | 0.0001 | 0.0033 | 0 | 0.001 | 0.0021 |
Mitochondria | 0.007 | 0.0002 | 0.0008 | 0.0096 | 0.0037 | 0.0184 | 0.0056 | 0.0002 | 0.0026 | 0.0017 | 0.0045 | 0.0016 | 0.0016 | 0.0001 | 0.0018 | 0.0004 | 0.0093 | 0.0014 |
None | 0.4766 | 0.5384 | 0.4908 | 0.5613 | 0.2757 | 0.3511 | 0.3411 | 0.3208 | 0.3329 | 0.3616 | 0.3057 | 0.2398 | 0.5045 | 0.4487 | 0.4644 | 0.2656 | 0.3574 | 0.3384 |
Nuclear Periphery | 0.0198 | 0.0007 | 0.0015 | 0.0029 | 0.0025 | 0.0019 | 0.0208 | 0.0005 | 0.0054 | 0.0015 | 0.0011 | 0.0003 | 0.0017 | 0.0003 | 0.0004 | 0.0008 | 0.0188 | 0.0005 |
Nucleolus | 0.0013 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0.0004 | 0.0005 | 0.0017 | 0 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
Nucleus | 0.0078 | 0.0009 | 0.0019 | 0.0008 | 0.001 | 0.0013 | 0.0124 | 0.0011 | 0.0045 | 0.0029 | 0.0018 | 0.0012 | 0.0012 | 0.0005 | 0.0012 | 0.002 | 0.0023 | 0.0012 |
Peroxisomes | 0.0148 | 0.0002 | 0.0006 | 0.0386 | 0.0054 | 0.0004 | 0.0037 | 0.0001 | 0.0003 | 0.0035 | 0.0091 | 0.0001 | 0.0158 | 0 | 0.0017 | 0.0001 | 0.0001 | 0.0011 |
Punctate Nuclear | 0.0083 | 0.0011 | 0.0031 | 0.0014 | 0.0023 | 0.0011 | 0.0032 | 0.0008 | 0.0025 | 0.0015 | 0.0065 | 0.0003 | 0.0016 | 0.0002 | 0.0003 | 0.0007 | 0.0008 | 0.0008 |
Vacuole | 0.0089 | 0.0006 | 0.0005 | 0.0011 | 0.0006 | 0.001 | 0.0016 | 0.0004 | 0.0006 | 0.0043 | 0.0007 | 0.0003 | 0.0007 | 0.0002 | 0.0005 | 0.0006 | 0.002 | 0.0003 |
Vacuole Periphery | 0.0032 | 0.0001 | 0.0001 | 0.0004 | 0.0005 | 0.0013 | 0.0015 | 0.0001 | 0.0003 | 0.0009 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0033 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.7101 | 11.5655 | 12.2602 | 14.6953 | 19.1707 | 11.0834 | 20.0467 | 15.2215 | 20.0108 | 24.7621 |
Translational Efficiency | 1.1351 | 2.2461 | 1.1638 | 1.6729 | 1.7801 | 2.709 | 1.2296 | 2.3255 | 1.5158 | 1.4013 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|