Standard name | |
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Human Ortholog | |
Description | Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | 1 | 0 | 0 | 0 | – | 2 | 1 | 2 | 2 | 35 | 23 | 19 | 1 | 3 | 0 | 1 | 1 | 5 | 2 | 6 | 9 |
Bud | 0 | 3 | 1 | 1 | 0 | 1 | 0 | – | 7 | 9 | 8 | 7 | 29 | 7 | 7 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 3 | 7 |
Bud Neck | 0 | 0 | 4 | 0 | 4 | 3 | 2 | – | 5 | 0 | 3 | 6 | 0 | 2 | 0 | 1 | 1 | 0 | 1 | 1 | 4 | 5 | 7 | 8 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 6 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 6 | 9 | 18 | 11 | 8 | 14 | – | 5 | 4 | 10 | 12 | 16 | 11 | 29 | 11 | 18 | 8 | 0 | 2 | 10 | 6 | 8 | 3 |
Cytoplasm | 46 | 194 | 260 | 180 | 253 | 229 | 346 | – | 207 | 207 | 228 | 268 | 234 | 161 | 330 | 184 | 164 | 90 | 65 | 191 | 227 | 155 | 261 | 269 |
Endoplasmic Reticulum | 1 | 1 | 2 | 0 | 0 | 0 | 3 | – | 2 | 2 | 3 | 1 | 2 | 0 | 0 | 17 | 7 | 5 | 0 | 0 | 1 | 2 | 7 | 4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 2 | 0 | 3 | 0 | 2 | 0 | 1 | 3 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 1 | 0 | 0 | 2 | 2 | 14 | 43 | – | 62 | 73 | 129 | 90 | 3 | 3 | 0 | 4 | 0 | 2 | 0 | 2 | 7 | 6 | 12 | 6 |
Nucleus | 0 | 0 | 2 | 0 | 1 | 0 | 2 | – | 1 | 4 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 11 | 23 | 19 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 3 | 0 | 1 | 1 | 1 | – | 4 | 1 | 6 | 5 | 9 | 21 | 10 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 0 | 0 | 1 | – | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 3 | 2 | 4 | 2 | 0 | 1 | 3 | 8 | 5 |
Unique Cell Count | 47 | 197 | 266 | 181 | 255 | 237 | 366 | 252 | 254 | 306 | 337 | 296 | 217 | 373 | 193 | 172 | 105 | 74 | 207 | 270 | 188 | 330 | 325 | |
Labelled Cell Count | 48 | 204 | 285 | 202 | 272 | 256 | 413 | 296 | 303 | 393 | 393 | 348 | 254 | 416 | 224 | 201 | 117 | 74 | 207 | 270 | 188 | 330 | 325 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.1 | 5.6 | 5.6 | 5.7 | 4.6 | 4.4 | – | 3.8 | 3.6 | 3.4 | 4.0 | 5.0 | 5.0 | 5.3 | 6.4 | 7.1 | 7.5 | 4.9 | 4.9 | 5.4 |
Std Deviation (1e-4) | 0.5 | 0.8 | 0.8 | 2.0 | 1.5 | 1.8 | 1.3 | – | 1.3 | 1.2 | 0.5 | 1.0 | 0.9 | 0.7 | 1.2 | 1.0 | 1.2 | 1.6 | 0.8 | 1.1 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.03 | -0.26 | -0.35 | – | -0.55 | -0.63 | -0.72 | -0.47 | -0.15 | -0.15 | -0.08 | 0.2 | 0.35 | 0.42 | -0.19 | -0.18 | -0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 5.3 | 4.9 | 3.0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 5.0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.9 | 0.6 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1.2 | 0.9 | 2.3 | 1.2 | 3.0 | 0 |
Cytoplasm | 1.4 | 1.4 | -0.8 | -2.0 | – | -6.0 | -6.1 | -7.8 | -6.7 | -6.8 | -7.7 | -4.3 | -1.4 | -1.4 | -4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.3 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3.2 | 5.4 | 3.7 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1.6 | 4.3 | 1.4 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.4709 | 0.9364 | 0.8649 | 0.9218 | 0.7454 | 1.0118 | 0.7062 | 1.239 | 1.0884 | 1.0614 | 0.5501 | 0.8868 | 1.0675 | 1.4217 | 1.1577 | 1.6305 | 1.5725 | 1.3997 |
Actin | 0.0732 | 0.0056 | 0.0232 | 0.0033 | 0.0659 | 0.0152 | 0.0241 | 0.0034 | 0.0302 | 0.0088 | 0.0123 | 0.0164 | 0.0548 | 0.0091 | 0.0139 | 0.0149 | 0.0957 | 0.0076 |
Bud | 0.0013 | 0.0022 | 0.0023 | 0.0114 | 0.0014 | 0.0002 | 0.0021 | 0.0004 | 0.0027 | 0.0055 | 0.0002 | 0.0008 | 0.0014 | 0.0008 | 0.0025 | 0.0052 | 0.0003 | 0.0003 |
Bud Neck | 0.0141 | 0.0016 | 0.009 | 0.0106 | 0.0019 | 0.0046 | 0.0056 | 0.0005 | 0.002 | 0.0097 | 0.0082 | 0.0067 | 0.0012 | 0.0004 | 0.0004 | 0.0025 | 0.0016 | 0.0018 |
Bud Periphery | 0.0038 | 0.0048 | 0.004 | 0.0159 | 0.0031 | 0.0003 | 0.0036 | 0.0005 | 0.0045 | 0.009 | 0.0002 | 0.003 | 0.0026 | 0.0007 | 0.0027 | 0.0099 | 0.0002 | 0.0007 |
Bud Site | 0.0086 | 0.0257 | 0.0251 | 0.0181 | 0.0029 | 0.0009 | 0.0045 | 0.0046 | 0.0187 | 0.0026 | 0.001 | 0.0004 | 0.0063 | 0.0215 | 0.0068 | 0.0567 | 0.0007 | 0 |
Cell Periphery | 0.0023 | 0.0026 | 0.0037 | 0.0015 | 0.0007 | 0.0005 | 0.0018 | 0.0017 | 0.0019 | 0.0026 | 0.0002 | 0.0005 | 0.0011 | 0.0019 | 0.0015 | 0.0027 | 0.0002 | 0.0002 |
Cytoplasm | 0.3422 | 0.6014 | 0.5725 | 0.4228 | 0.4135 | 0.6825 | 0.5922 | 0.8336 | 0.7067 | 0.6814 | 0.7422 | 0.8245 | 0.5579 | 0.7266 | 0.6839 | 0.5246 | 0.6698 | 0.7546 |
Cytoplasmic Foci | 0.0339 | 0.0174 | 0.0351 | 0.1251 | 0.0046 | 0.011 | 0.0264 | 0.0054 | 0.0201 | 0.0393 | 0.0566 | 0.007 | 0.051 | 0.006 | 0.014 | 0.0232 | 0.0187 | 0.0036 |
Eisosomes | 0.0011 | 0.0004 | 0.0014 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0099 | 0.0041 | 0.004 | 0.0032 | 0.0051 | 0.0028 | 0.008 | 0.0042 | 0.0038 | 0.0038 | 0.0011 | 0.0017 | 0.0047 | 0.0027 | 0.0042 | 0.0101 | 0.0036 | 0.0027 |
Endosome | 0.03 | 0.0014 | 0.0122 | 0.0132 | 0.0373 | 0.008 | 0.0468 | 0.0032 | 0.013 | 0.0328 | 0.0059 | 0.0061 | 0.0342 | 0.003 | 0.0129 | 0.0773 | 0.0761 | 0.0018 |
Golgi | 0.0103 | 0.0005 | 0.0044 | 0.0035 | 0.0151 | 0.0189 | 0.0142 | 0.0035 | 0.0075 | 0.0138 | 0.0118 | 0.0038 | 0.016 | 0.0037 | 0.008 | 0.0284 | 0.0097 | 0.0005 |
Lipid Particles | 0.0222 | 0.0009 | 0.0067 | 0.0086 | 0.0019 | 0.003 | 0.0325 | 0.0015 | 0.002 | 0.003 | 0.0095 | 0.0022 | 0.0223 | 0.0003 | 0.0017 | 0.0205 | 0.0017 | 0.0001 |
Mitochondria | 0.0204 | 0.0002 | 0.0012 | 0.0014 | 0.0525 | 0.0171 | 0.015 | 0.001 | 0.022 | 0.021 | 0.0399 | 0.0067 | 0.0109 | 0.0006 | 0.0138 | 0.0162 | 0.0013 | 0.0001 |
None | 0.3128 | 0.3266 | 0.277 | 0.3369 | 0.3159 | 0.231 | 0.1856 | 0.1325 | 0.156 | 0.1204 | 0.104 | 0.1147 | 0.1984 | 0.22 | 0.2251 | 0.1851 | 0.1094 | 0.2076 |
Nuclear Periphery | 0.0324 | 0.0009 | 0.0029 | 0.0012 | 0.0238 | 0.0008 | 0.0092 | 0.0004 | 0.0008 | 0.002 | 0.0002 | 0.0007 | 0.0032 | 0.0003 | 0.0007 | 0.0039 | 0.0006 | 0.0003 |
Nucleolus | 0.0053 | 0.0002 | 0.0003 | 0.0027 | 0.0003 | 0.0001 | 0.001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0007 | 0 | 0 |
Nucleus | 0.0261 | 0.0009 | 0.0015 | 0.0027 | 0.0411 | 0.0004 | 0.003 | 0.0006 | 0.001 | 0.0013 | 0.0006 | 0.0007 | 0.0008 | 0.0005 | 0.0007 | 0.0022 | 0.0004 | 0.0003 |
Peroxisomes | 0.0114 | 0.0006 | 0.007 | 0.0048 | 0.0007 | 0.0007 | 0.0134 | 0.0015 | 0.003 | 0.0116 | 0.0042 | 0.0025 | 0.0205 | 0.0003 | 0.004 | 0.0031 | 0.0059 | 0.0001 |
Punctate Nuclear | 0.0303 | 0.0006 | 0.0049 | 0.0093 | 0.0005 | 0.0006 | 0.002 | 0.0002 | 0.0011 | 0.0005 | 0.001 | 0.0006 | 0.0078 | 0.0003 | 0.0007 | 0.0015 | 0.0003 | 0.0001 |
Vacuole | 0.0048 | 0.0013 | 0.0014 | 0.0032 | 0.0078 | 0.0005 | 0.0059 | 0.001 | 0.0019 | 0.0219 | 0.0005 | 0.0006 | 0.0026 | 0.0011 | 0.0017 | 0.0079 | 0.0024 | 0.0163 |
Vacuole Periphery | 0.0039 | 0.0001 | 0.0003 | 0.0004 | 0.0038 | 0.0007 | 0.0027 | 0.0001 | 0.0006 | 0.0087 | 0.0003 | 0.0002 | 0.0012 | 0.0001 | 0.0004 | 0.0028 | 0.001 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.0637 | 11.3226 | 14.2186 | 10.7884 | 7.8952 | 10.7115 | 11.5584 | 13.3734 | 13.4186 | 11.0591 |
Translational Efficiency | 0.9535 | 0.8305 | 0.6319 | 0.9041 | 0.7842 | 0.6286 | 0.7039 | 0.756 | 0.6979 | 0.6982 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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