Standard name
Human Ortholog
Description Protein involved in the synthesis of GlcNAc-PI; GlcNAc-PI is the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein; GlcNAc-PI stands for N-acetylglucosaminyl phosphatidylinositol
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.07 0.11 0.3 0.09 0 0 0 0 0 0
Cytoplasm 0.37 0.49 0.53 0.53 0.68 0.54 0.6 0.54 0.48 0.58 0.59 0.48 0.72 0.62 0.76 0.78 0.65 0.64 0.08 0.1 0.11 0.19 0.19 0.16
Endoplasmic Reticulum 0.23 0.45 0.28 0.18 0.07 0.13 0 0 0 0 0 0.05 0.23 0.34 0.21 0.06 0.1 0 0.61 0.73 0.64 0.51 0.44 0.43
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.52 0 0.28 0.3 0.31 0.45 0.55 0.62 0.66 0.56 0.61 0.66 0 0 0 0 0.05 0.14 0.13 0.06 0.12 0.16 0.17 0.18
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.2 0.12 0 0.05 0 0.07 0.1 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 1 4 0 1 0 0 0 0 0 0 1 2 1 2 0 4 3 2 4 2 2 3
Bud 1 1 1 2 2 4 12 7 14 18 22 18 1 0 1 0 0 1 0 0 0 0 1 3
Bud Neck 0 0 0 0 0 1 1 0 1 1 0 0 0 1 0 0 0 0 1 2 2 2 1 1
Bud Site 0 0 0 0 0 0 0 2 3 3 3 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 1 5 3 3 4 3 8 5 4 10 3 20 18 2 6 5 1 0 5 1 2 1
Cytoplasm 52 74 105 103 153 170 305 208 248 307 271 272 56 131 205 14 13 37 13 18 28 26 31 27
Endoplasmic Reticulum 32 69 55 35 16 40 6 2 22 1 1 26 18 72 56 1 2 0 102 140 162 70 70 72
Endosome 0 4 0 2 0 0 2 0 0 2 0 1 0 0 0 0 0 0 4 1 2 0 2 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Mitochondria 73 2 56 59 69 140 281 239 342 300 280 375 0 0 1 0 1 8 21 12 30 21 27 31
Nucleus 5 0 0 0 1 2 5 1 4 2 5 4 0 0 1 0 0 3 1 0 0 0 0 0
Nuclear Periphery 2 0 0 0 1 1 3 1 10 2 1 3 0 0 0 0 0 1 4 0 1 0 0 0
Nucleolus 0 0 0 0 1 0 2 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 0 1 3 2 0 0 0 0 2 0 0 0 0 1 0 0 1
Vac/Vac Membrane 10 11 4 6 9 9 21 11 14 20 13 11 3 5 11 1 4 7 3 9 10 9 16 22
Unique Cell Count 140 152 199 194 225 312 510 385 519 532 462 569 78 210 268 18 20 58 166 192 255 138 160 168
Labelled Cell Count 178 164 223 216 255 371 642 474 667 665 604 720 82 231 294 22 26 66 166 192 255 138 160 168


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 6.0 4.0 4.3 3.9 3.6 3.3 3.4 3.0 3.1 3.2 3.2 6.1 6.1 6.2 6.6 6.9 6.8 6.5 6.7 6.8
Std Deviation (1e-4) 1.3 0.8 0.6 1.2 1.0 0.9 1.2 1.1 0.5 0.8 0.7 0.6 0.7 0.7 1.0 1.2 0.9 1.0 0.9 1.1 1.3
Intensity Change (Log2) – – – 0.11 -0.03 -0.12 -0.28 -0.22 -0.4 -0.34 -0.33 -0.31 0.63 0.63 0.65 0.73 0.8 0.78 0.72 0.76 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 4.1 3.4 0 0 0
Cytoplasm 0.1 3.2 0.4 1.7 0.3 -1.2 1.2 1.4 -1.2 2.9 2.0 5.4 2.0 1.0 1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 -0.8 1.5 -1.7 0 -1.7 -4.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -5.2 -8.3 -9.1 0 -2.2 -2.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.2 0.6 1.4 0.6 0 1.2 0.6 0 0 0 1.3 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8532 0.5372 0.6055 0.5267 0.5753 0.4909 2.2477 2.5107 2.2622 2.1207 2.2845 2.6037 0.0952 0.0293 -0.0795 0.1298 -0.388 -0.2882
Actin 0.0106 0.0029 0.0036 0.0177 0.0199 0.0023 0.0138 0.0009 0.001 0.0385 0.0078 0.0037 0.0343 0.0015 0.0061 0.0034 0.0045 0.0012
Bud 0.0005 0.0006 0.0002 0.0005 0.0034 0.0001 0.0001 0.0002 0.0001 0.0002 0.0007 0.0004 0.0002 0.0001 0.0002 0.0004 0.0045 0
Bud Neck 0.002 0.0004 0.0039 0.0022 0.0018 0.0014 0.0006 0.0005 0.0038 0.0008 0.0024 0.0031 0.0065 0.0002 0.0015 0.0004 0.0051 0.0044
Bud Periphery 0.0014 0.0012 0.0003 0.002 0.0133 0.0003 0.0002 0.0003 0.0002 0.0003 0.0012 0.0017 0.0005 0.0001 0.0005 0.001 0.0118 0.0002
Bud Site 0.0044 0.0139 0.0029 0.002 0.0084 0.0002 0.0011 0.0202 0.0019 0.0002 0.0112 0.0002 0.0022 0.0011 0.0023 0.0009 0.0029 0.0001
Cell Periphery 0.0007 0.001 0.001 0.0007 0.0006 0.0003 0.0015 0.0014 0.0015 0.0004 0.0006 0.0005 0.0016 0.0008 0.0011 0.0006 0.0009 0.0006
Cytoplasm 0.2389 0.3094 0.3093 0.3302 0.3615 0.3623 0.2762 0.2748 0.3048 0.2206 0.278 0.3076 0.2573 0.3939 0.338 0.2601 0.4209 0.4075
Cytoplasmic Foci 0.0233 0.0183 0.0225 0.0173 0.0372 0.018 0.0116 0.01 0.0116 0.0119 0.0077 0.0112 0.0175 0.01 0.0111 0.0256 0.0152 0.0115
Eisosomes 0.0005 0.0003 0.0003 0.0007 0.0005 0.0001 0.0008 0.0004 0.0003 0.0003 0.0004 0.0001 0.0009 0.0001 0.0004 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.237 0.1781 0.1678 0.1134 0.1169 0.1536 0.2957 0.2408 0.2681 0.2265 0.2782 0.2389 0.3097 0.2585 0.2366 0.3823 0.2414 0.2449
Endosome 0.0454 0.0307 0.0324 0.0377 0.06 0.024 0.0169 0.0181 0.0223 0.0159 0.0134 0.0146 0.0208 0.0185 0.0153 0.0247 0.0186 0.0163
Golgi 0.0082 0.0024 0.0048 0.0037 0.0102 0.0025 0.0018 0.0006 0.0012 0.0051 0.0015 0.0009 0.0059 0.0027 0.002 0.0045 0.0008 0.0018
Lipid Particles 0.031 0.0055 0.0089 0.0219 0.0362 0.008 0.0085 0.0072 0.0114 0.0067 0.0047 0.0045 0.0192 0.0115 0.0059 0.0377 0.013 0.0132
Mitochondria 0.0078 0.005 0.0048 0.0063 0.018 0.0023 0.0009 0.0006 0.001 0.0014 0.0019 0.0013 0.0036 0.004 0.0075 0.0014 0.0016 0.0051
None 0.3083 0.3669 0.3856 0.3441 0.2276 0.3753 0.3313 0.3757 0.3401 0.4048 0.3569 0.3714 0.2805 0.2688 0.3453 0.2064 0.2114 0.2649
Nuclear Periphery 0.0299 0.0285 0.0202 0.034 0.0312 0.0242 0.0216 0.0242 0.0162 0.0515 0.0141 0.0198 0.0176 0.015 0.012 0.0204 0.0267 0.0124
Nucleolus 0.0026 0.0008 0.0011 0.0032 0.002 0.0005 0.0005 0.0013 0.0003 0.0002 0.0013 0.0004 0.001 0.0002 0.0003 0.0007 0.0005 0.0002
Nucleus 0.0116 0.0142 0.01 0.0167 0.0121 0.0126 0.0061 0.0081 0.0047 0.007 0.005 0.0079 0.0051 0.0053 0.0037 0.0051 0.0082 0.0054
Peroxisomes 0.0143 0.0037 0.0021 0.0053 0.0086 0.0007 0.0005 0.0003 0.0003 0.0006 0.0033 0.0005 0.0038 0.001 0.003 0.0143 0.0012 0.0038
Punctate Nuclear 0.0132 0.0076 0.009 0.031 0.018 0.0062 0.0034 0.0075 0.0019 0.0021 0.0042 0.0069 0.0062 0.0015 0.0028 0.0035 0.0056 0.0016
Vacuole 0.0063 0.0073 0.0077 0.0079 0.0088 0.0039 0.006 0.0061 0.0063 0.0036 0.0046 0.0036 0.0049 0.0049 0.0039 0.0054 0.0042 0.004
Vacuole Periphery 0.002 0.0014 0.0015 0.0016 0.0038 0.001 0.0008 0.0007 0.0008 0.0013 0.0007 0.0008 0.0008 0.0007 0.0007 0.0011 0.001 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.2356 3.5102 6.946 9.7014 3.3941 5.2327 11.8307 5.1902 7.0857 10.0206
Translational Efficiency 0.8182 1.4186 0.8698 0.4688 1.6199 0.9507 0.5469 0.882 0.8075 0.7626

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in the synthesis of GlcNAc-PI; GlcNAc-PI is the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein; GlcNAc-PI stands for N-acetylglucosaminyl phosphatidylinositol
Localization
Cell Percentages ER (79%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Gpi2

Gpi2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gpi2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available