Standard name
Human Ortholog
Description COPII vesicle coat protein required for ER transport vesicle budding; essential factor in endoplasmic reticulum exit site (ERES) formation, as well as in COPII-mediated ER-to-Golgi traffic; bound to periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.57 0.4 0.6 0.54 0.48 0.43 0.25 0.19 0.2 0.15 0.11 0.14 0.51 0.5 0.45 0.45 0.41 0.45 0.46 0.29 0.49 0.5 0.6 0.52
Bud 0 0 0.13 0.13 0.15 0.09 0.15 0.09 0.08 0.08 0.11 0.09 0.05 0 0.08 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0.08 0.12 0.1 0.08 0 0 0 0.05 0 0.05 0.08 0 0.06 0.09 0.09 0.12 0 0 0 0 0 0
Cytoplasm 0 0.13 0.07 0.07 0.05 0.06 0.07 0 0 0.05 0 0 0.09 0.09 0.06 0.1 0.14 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0.45 0.75 0.41 0.3 0.3 0.39 0.2 0.2 0.22 0.17 0.2 0.16 0.64 0.52 0.45 0.09 0.07 0 0.28 0.38 0.18 0.26 0.21 0.27
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.62 0.06 0.09 0 0.06 0 0 0 0 0 0 0 0.25 0.31 0.16 0.74 0.67 0.67 0.19 0.18 0.18 0.1 0.11 0.12
Mitochondria 0.06 0.07 0.26 0.62 0.42 0.59 0.72 0.76 0.83 0.83 0.85 0.87 0 0.11 0.34 0.05 0.08 0.08 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0 0.12 0 0.06 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 189 111 285 199 210 261 173 147 123 100 56 97 99 126 117 54 43 53 171 85 270 73 129 100
Bud 11 10 63 47 66 53 101 71 48 54 56 60 9 11 22 0 1 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 1 1 1 7 1 1 0 0 0 0 0 0
Cell Periphery 14 13 40 44 42 49 31 21 21 36 22 37 16 9 17 11 10 14 2 2 13 2 5 7
Cytoplasm 9 37 31 24 23 35 48 29 27 34 19 21 18 23 17 12 15 20 2 6 2 3 0 0
Endoplasmic Reticulum 149 210 195 109 130 236 139 153 136 110 98 111 124 132 117 11 7 4 104 112 97 39 44 52
Endosome 9 3 15 0 16 5 23 21 2 2 0 2 1 1 3 3 1 0 6 6 8 1 1 1
Golgi 207 18 41 9 25 5 6 1 3 2 3 3 49 78 43 88 71 79 71 51 97 15 22 23
Mitochondria 21 19 126 227 183 358 496 576 502 546 415 611 5 28 88 6 9 10 4 12 28 6 4 2
Nucleus 0 0 0 0 0 1 0 0 3 1 1 0 3 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 3 0 2 1 2 3 2 1 0 4 1 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 26 4 52 16 40 41 12 38 18 24 3 5 5 1 0 1 3 1 17 0 1 0
SpindlePole 0 0 0 0 4 0 4 3 1 6 7 2 0 1 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 4 1 2 0 2 1 3 18 0 2 1 2 0 1 1 1 3 2 0 5 2 2 0 0
Unique Cell Count 333 281 476 367 433 603 691 762 607 656 490 702 194 254 262 119 106 118 369 293 546 149 214 193
Labelled Cell Count 614 423 824 663 753 1024 1065 1085 880 941 701 975 328 415 434 188 160 185 369 293 546 149 214 193


ER to Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.6 10.3 7.6 6.8 7.2 6.3 6.0 5.9 5.3 5.2 4.9 4.8 10.6 10.4 9.3 12.1 14.0 13.0 10.9 12.1 12.4
Std Deviation (1e-4) 1.9 1.7 1.0 1.1 1.0 0.9 0.9 1.0 1.0 1.0 0.9 0.9 1.7 1.9 1.6 3.0 2.5 2.6 2.2 2.0 2.1
Intensity Change (Log2) -0.15 -0.07 -0.26 -0.33 -0.35 -0.52 -0.54 -0.62 -0.65 0.48 0.46 0.31 0.68 0.89 0.79 0.52 0.68 0.71


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.6 -3.4 -5.4 -12.0 -14.6 -13.4 -15.6 -15.8 0 -2.1 -2.7 -4.0 -2.9 -3.6 -2.9
Bud -0.2 0.9 -2.3 0.7 -2.2 -2.9 -2.7 -0.9 0 -3.3 -3.8 -2.0 -4.2 -3.7 -3.9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.7 0.7 -0.2 -2.8 -4.5 -3.5 -1.9 -2.5 0 -0.1 -2.5 -0.9 0.3 0.3 1.2
Cytoplasm 0 -0.8 -0.5 0.3 -2.2 -1.5 -0.9 -1.8 -2.9 1.2 1.3 0 1.3 2.6 3.6
Endoplasmic Reticulum -3.4 -3.4 -0.6 -7.7 -8.0 -6.6 -9.1 -7.1 0 5.4 2.8 1.0 -6.5 -6.7 -7.7
Endosome -3.4 0.5 -2.8 0.2 -0.4 -3.7 -3.9 -4.0 0 0 -2.4 -1.7 0 0 0
Golgi -3.8 -1.6 -6.3 -6.6 -8.0 -6.7 -7.2 -6.0 0 5.7 7.7 3.2 15.5 13.8 14.1
Mitochondria 10.3 5.0 10.8 15.2 17.0 18.6 19.2 18.2 0 -7.1 -4.9 2.0 -5.0 -4.0 -4.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -3.4 3.5 -2.4 0.2 -0.1 -3.1 0.2 -1.3 0 -2.3 -2.2 -2.3 -2.2 0 -2.2
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.6 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.5791 8.7948 7.9992 6.842 7.6838 8.1845 8.8559 11.4853 10.374 9.0988 9.5807 10.4539 10.8078 11.8212 11.0547 10.8076 11.0423 11.2234
Actin 0.2528 0.2018 0.1973 0.2067 0.0788 0.3162 0.202 0.2873 0.235 0.2415 0.169 0.2004 0.1431 0.1901 0.1943 0.1294 0.3093 0.2294
Bud 0.0006 0.0003 0.0006 0.0007 0.0024 0.0003 0.0005 0.0029 0.0005 0.0003 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002 0.0003
Bud Neck 0.0081 0.0019 0.0021 0.0029 0.0012 0.0076 0.0084 0.0021 0.0015 0.0011 0.0014 0.0033 0.001 0.0014 0.0015 0.0021 0.0039 0.0031
Bud Periphery 0.0008 0.0003 0.0015 0.0011 0.0024 0.0004 0.0005 0.0007 0.0004 0.0003 0.0002 0.0003 0.0004 0.0002 0.0002 0.0001 0.0007 0.0005
Bud Site 0.0134 0.0033 0.0035 0.0015 0.0052 0.0006 0.0107 0.0186 0.0055 0.0004 0.0003 0.0002 0.0012 0.0007 0.0004 0.0002 0.0007 0.0033
Cell Periphery 0.0013 0.002 0.0018 0.0033 0.0006 0.0013 0.0011 0.001 0.0009 0.0015 0.0006 0.0013 0.0008 0.0012 0.0014 0.0027 0.0021 0.0012
Cytoplasm 0.0234 0.024 0.0147 0.004 0.0027 0.0065 0.0085 0.0269 0.0128 0.0047 0.006 0.007 0.0032 0.0097 0.0079 0.0067 0.0037 0.0113
Cytoplasmic Foci 0.029 0.0431 0.0311 0.0296 0.0156 0.0232 0.0392 0.0465 0.034 0.0294 0.026 0.0263 0.0251 0.025 0.0159 0.0257 0.018 0.0178
Eisosomes 0.0025 0.0033 0.0013 0.0036 0.0004 0.002 0.0022 0.0009 0.002 0.0024 0.0011 0.0047 0.003 0.0036 0.0113 0.0068 0.0169 0.0064
Endoplasmic Reticulum 0.0268 0.0329 0.0287 0.0138 0.0145 0.0144 0.0162 0.0244 0.0078 0.0141 0.0348 0.0355 0.005 0.0114 0.008 0.0432 0.0073 0.0199
Endosome 0.0576 0.0546 0.0508 0.0867 0.039 0.077 0.05 0.047 0.0308 0.0376 0.0686 0.0461 0.0402 0.0274 0.0231 0.0273 0.0228 0.0281
Golgi 0.4917 0.5802 0.6098 0.5491 0.7522 0.5105 0.5754 0.5212 0.6394 0.5947 0.662 0.6432 0.7333 0.7177 0.7191 0.7183 0.6053 0.6621
Lipid Particles 0.0564 0.0477 0.0391 0.0265 0.0667 0.0323 0.0189 0.0164 0.0147 0.0595 0.0195 0.0184 0.0203 0.0084 0.0132 0.0122 0.0043 0.0068
Mitochondria 0.0098 0.0013 0.0074 0.0623 0.0136 0.0022 0.0066 0.0014 0.0024 0.0052 0.0055 0.0077 0.0092 0.0015 0.0015 0.0041 0.0035 0.007
None 0.0046 0.0002 0.001 0.0004 0.0002 0.0001 0.0054 0.0002 0.0002 0.0003 0.0002 0.0003 0.0021 0.0001 0.0001 0.0002 0.0002 0.0004
Nuclear Periphery 0.0032 0.0006 0.0013 0.0012 0.0009 0.0005 0.0058 0.0003 0.0002 0.0003 0.0003 0.0007 0.001 0.0002 0.0002 0.0001 0.0002 0.0003
Nucleolus 0.0006 0 0.0001 0.0001 0.0001 0 0.0166 0 0 0 0 0 0.0004 0 0 0 0 0
Nucleus 0.0055 0.0001 0.0003 0.0002 0.0004 0.0001 0.0146 0.0001 0 0 0.0001 0.0001 0.0003 0 0 0 0 0.0001
Peroxisomes 0.0064 0.001 0.005 0.0026 0.0013 0.0027 0.0042 0.0011 0.0112 0.0057 0.0018 0.0034 0.0071 0.0006 0.0011 0.0202 0.0006 0.0011
Punctate Nuclear 0.0027 0.0002 0.0004 0.0005 0.0003 0.0002 0.0111 0.0002 0.0003 0.0003 0.0001 0.0002 0.001 0.0001 0.0001 0.0001 0.0001 0.0001
Vacuole 0.0015 0.0006 0.0014 0.0016 0.0007 0.0009 0.0011 0.0004 0.0002 0.0004 0.0012 0.0004 0.0013 0.0002 0.0002 0.0002 0.0002 0.0003
Vacuole Periphery 0.0013 0.0005 0.0008 0.0018 0.0009 0.0009 0.0011 0.0003 0.0002 0.0004 0.0014 0.0006 0.0008 0.0002 0.0003 0.0002 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.2412 47.0131 55.3283 58.6992 45.6024 45.0291 57.0721 58.6993 48.9609 55.2699
Translational Efficiency 1.8038 1.7512 1.3619 1.1771 1.4576 1.6381 1.1259 1.1045 1.2582 1.1494

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description COPII vesicle coat protein required for ER transport vesicle budding; essential factor in endoplasmic reticulum exit site (ERES) formation, as well as in COPII-mediated ER-to-Golgi traffic; bound to periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p
Localization
Cell Percentages ER (93%)
Cell Cycle Regulation No
Subcompartmental Group ER-1

Sec16

Sec16


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec16-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available