Standard name
Human Ortholog
Description Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; cytosolic Glr1p is the main determinant of the glutathione redox state of the mitochondrial intermembrane space; mitochondrial Glr1p has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.97 0.93 0.91 0.9 0.78 0.72 0.64 0.64 0.66 0.65 0.65 0.97 0.99 0.99 0.98 0.96 0.96 0.82 0.83 0.71 0.7 0.74 0.66
Endoplasmic Reticulum 0 0 0 0 0.07 0.05 0 0.1 0.1 0.06 0 0.06 0 0 0 0 0 0 0 0 0.08 0.06 0 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0 0.07 0.12 0.11 0.19 0.2 0.25 0.22 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0 0.12 0.15 0.14 0 0.08 0.06 0.06 0 0 0 0 0 0 0 0.06 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 0 1 2 3 2 4 8 10 7 3 15 1 0 0 2 0 0 2 1 1 1 4 5
Bud 1 0 0 0 0 4 2 8 3 7 4 4 0 0 0 1 0 0 1 1 0 1 0 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 1 1 3 1 3 6 9 11 7 3 10 4 5 5 8 6 5 2 0 1 0 1 1
Cytoplasm 284 77 99 95 152 115 142 185 123 134 100 142 233 335 358 287 151 124 260 81 78 83 191 165
Endoplasmic Reticulum 5 2 1 2 11 7 7 28 20 13 5 12 4 4 2 12 5 5 9 0 8 6 8 15
Endosome 2 0 0 0 3 4 3 7 6 1 1 3 0 0 0 0 0 0 11 4 5 4 16 13
Golgi 2 0 0 0 0 0 0 3 1 2 1 0 1 1 0 0 0 0 9 0 0 5 2 7
Mitochondria 8 0 4 8 2 10 23 33 36 40 39 48 0 1 1 1 2 1 2 0 5 3 4 7
Nucleus 1 0 1 0 0 0 0 1 0 0 1 1 0 0 1 4 0 2 0 0 0 0 2 1
Nuclear Periphery 0 0 0 3 0 0 0 0 0 1 0 1 0 0 0 0 1 0 4 0 0 1 4 2
Nucleolus 0 0 0 0 0 1 1 1 0 2 1 0 0 0 0 0 1 0 0 0 0 1 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 0 0 0 3 3
Vac/Vac Membrane 3 0 1 5 6 18 30 41 5 16 10 13 6 1 1 6 4 3 3 5 5 3 9 10
Unique Cell Count 304 79 107 104 169 148 196 288 191 203 154 217 241 340 361 293 158 129 316 98 111 119 259 251
Labelled Cell Count 313 80 108 118 178 164 218 324 215 230 168 249 249 347 368 322 170 140 316 98 111 119 259 251


Cytoplasm, Mitochondria, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.1 16.0 11.2 10.3 12.3 10.4 9.7 10.5 10.0 9.7 9.0 10.4 17.3 16.9 17.1 15.8 17.9 20.6 14.6 13.8 14.6
Std Deviation (1e-4) 2.7 3.2 2.5 2.8 2.8 2.6 2.6 2.3 2.5 2.3 2.4 2.5 3.7 4.2 4.0 3.8 5.2 4.5 3.8 2.9 3.4
Intensity Change (Log2) – – – -0.13 0.13 -0.11 -0.22 -0.1 -0.16 -0.21 -0.33 -0.12 0.62 0.59 0.6 0.49 0.67 0.87 0.38 0.3 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 2.3 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 -0.7 -3.2 -4.1 -5.6 -5.3 -5.1 -5.2 -5.3 1.7 3.2 4.0 2.6 1.1 1.2
Endoplasmic Reticulum 0 0 0 0 3.0 3.1 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.2 0 1.0 2.3 2.3 3.7 3.8 4.6 4.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.4 3.9 3.8 0 2.6 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.3061 21.1052 19.651 19.1946 18.6896 18.9409 12.5193 16.4427 12.8122 13.1213 12.4923 15.6007 15.3539 19.8417 18.1223 19.6719 14.6565 17.8447
Actin 0.0141 0.0084 0.0093 0.0047 0.0126 0.0095 0.0226 0.0333 0.0406 0.0565 0.0408 0.0278 0.0469 0.0074 0.0222 0.0243 0.0257 0.0092
Bud 0.0018 0.0012 0.0027 0.0024 0.0045 0.0013 0.0023 0.0012 0.0015 0.0027 0.0025 0.0019 0.0025 0.0013 0.0022 0.0042 0.0035 0.0012
Bud Neck 0.003 0.0011 0.0068 0.0024 0.0042 0.0171 0.0007 0.001 0.0014 0.0017 0.0024 0.0073 0.0017 0.0012 0.0018 0.0032 0.0031 0.0036
Bud Periphery 0.0014 0.0003 0.0023 0.0015 0.0022 0.001 0.0013 0.0003 0.0011 0.0019 0.0009 0.0004 0.0034 0.0005 0.0008 0.0018 0.0015 0.0007
Bud Site 0.0044 0.0022 0.0021 0.0012 0.0007 0.0004 0.0026 0.0075 0.0124 0.0029 0.0012 0.0004 0.0041 0.0015 0.008 0.002 0.0016 0.0004
Cell Periphery 0.0006 0.0002 0.0004 0.0003 0.0002 0.0002 0.0002 0.0002 0.0005 0.0003 0.0001 0.0001 0.0009 0.0002 0.0003 0.0002 0.0002 0.0001
Cytoplasm 0.7512 0.9427 0.8991 0.8807 0.9004 0.8563 0.7974 0.8921 0.7923 0.7387 0.6594 0.878 0.6737 0.9352 0.9009 0.8349 0.7796 0.8644
Cytoplasmic Foci 0.0237 0.0134 0.0103 0.0152 0.0105 0.0121 0.035 0.03 0.0291 0.0315 0.0525 0.0248 0.0482 0.0134 0.0241 0.0409 0.055 0.0259
Eisosomes 0.0006 0 0 0.0001 0 0 0.0001 0 0.0002 0.0001 0 0 0.0003 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0251 0.0042 0.0092 0.0045 0.0066 0.0188 0.0211 0.004 0.0051 0.0021 0.0046 0.0047 0.0322 0.0051 0.0033 0.009 0.016 0.014
Endosome 0.052 0.0082 0.0155 0.0177 0.0188 0.035 0.0434 0.0088 0.0355 0.0631 0.0704 0.0259 0.0726 0.0156 0.02 0.0335 0.0396 0.0397
Golgi 0.007 0.0037 0.0031 0.0046 0.0031 0.007 0.0122 0.0122 0.0232 0.0433 0.0271 0.0064 0.0125 0.0035 0.0046 0.0111 0.0092 0.0096
Lipid Particles 0.0062 0.0002 0.0028 0.002 0.0001 0.0022 0.0052 0.0004 0.0147 0.0064 0.0036 0.0047 0.008 0.0001 0.0003 0.0024 0.0064 0.0001
Mitochondria 0.012 0.0018 0.0094 0.0234 0.004 0.0068 0.0067 0.0012 0.0088 0.0287 0.1084 0.003 0.0116 0.0076 0.002 0.0065 0.0085 0.0122
None 0.0077 0.0006 0.0011 0.0124 0.0006 0.0008 0.0084 0.0005 0.0019 0.0006 0.0008 0.0027 0.0079 0.0004 0.0007 0.0008 0.0046 0.0006
Nuclear Periphery 0.0273 0.0034 0.008 0.0043 0.0073 0.0097 0.0095 0.0008 0.0029 0.0011 0.0041 0.0014 0.0255 0.0013 0.0011 0.0065 0.0148 0.0062
Nucleolus 0.0008 0 0.0004 0.0004 0.0001 0.0002 0.0006 0 0.0022 0.0001 0.0001 0.0001 0.0004 0 0.0001 0.0002 0.0002 0
Nucleus 0.0288 0.0035 0.0065 0.0063 0.0158 0.0085 0.0068 0.001 0.0027 0.001 0.002 0.0019 0.0192 0.0018 0.0015 0.003 0.0037 0.004
Peroxisomes 0.0055 0.0012 0.0015 0.0007 0.0004 0.0007 0.0081 0.0038 0.0155 0.0062 0.0141 0.005 0.0121 0.0007 0.0036 0.0032 0.0173 0.0016
Punctate Nuclear 0.0031 0.0003 0.0024 0.0037 0.0005 0.0018 0.0095 0.0003 0.0022 0.0008 0.0012 0.0013 0.0041 0.0002 0.0004 0.0015 0.0065 0.0002
Vacuole 0.0185 0.0027 0.0048 0.0081 0.0052 0.0073 0.0042 0.0013 0.004 0.0078 0.0017 0.0013 0.008 0.0021 0.0013 0.0057 0.0018 0.004
Vacuole Periphery 0.0052 0.0006 0.0025 0.0035 0.0021 0.0032 0.002 0.0002 0.0021 0.0026 0.0021 0.0009 0.004 0.0007 0.0006 0.0049 0.0013 0.0022

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 83.5912 85.5946 70.068 79.8101 83.2553 89.621 74.3528 79.0414 91.7134 84.0344
Translational Efficiency 1.8297 1.4267 1.4311 1.5424 1.4161 1.9248 1.7056 1.2358 1.2352 1.4057

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; cytosolic Glr1p is the main determinant of the glutathione redox state of the mitochondrial intermembrane space; mitochondrial Glr1p has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (84%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Glr1

Glr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Glr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available