Standard name
Human Ortholog
Description Conserved peptide N-glycanase; deglycosylating enzyme that cleaves N-glycans that are attached to misfolded ERAD substrate glycoproteins prior to proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p; human ortholog NGLY1 is associated with a syndrome characterized by developmental delays, epilepsy, absence of tears and liver disease
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.05 0 0 0 0 0 0
Cytoplasm 0.95 0.95 1.0 0.98 0.99 0.95 0.92 0.92 0.85 0.85 0.72 0.82 1.0 1.0 0.99 0.96 0.97 0.99 0.91 0.9 0.89 0.93 0.9 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.06 0 0 0 0.06 0.14 0.1 0.24 0.21 0.43 0.25 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 1 1 0 0 0 0 0 4 1 0 2 6 4
Bud 2 1 0 2 1 1 1 4 10 5 14 5 1 2 0 0 1 1 1 0 0 3 7 15
Bud Neck 0 0 1 3 5 2 4 4 1 3 1 5 1 0 0 0 0 0 0 0 0 1 1 5
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 0 4 4 6 5 3 2 6 1 4 1 1 3 12 17 14 0 0 0 0 1 2
Cytoplasm 198 81 41 294 513 357 455 477 326 350 218 273 441 553 616 209 193 303 190 80 78 296 489 560
Endoplasmic Reticulum 1 1 0 2 1 2 0 0 1 2 0 2 1 0 1 10 10 22 2 0 0 0 2 0
Endosome 0 0 0 0 1 0 0 0 2 0 0 1 0 1 2 0 1 1 1 2 0 0 2 4
Golgi 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 1 2 1 0 0 0 1 4
Mitochondria 15 5 0 0 3 22 71 52 93 87 130 83 1 1 1 4 0 7 2 1 4 7 9 6
Nucleus 0 0 0 2 0 1 4 3 3 8 3 4 0 1 1 2 1 0 0 0 0 1 0 0
Nuclear Periphery 1 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 4 0 2 1 3 0 0 0 0 0 0 0 0 0 0 5 5
Vac/Vac Membrane 1 1 0 1 1 4 2 5 10 9 5 9 2 1 2 3 1 1 0 0 0 1 1 2
Unique Cell Count 208 85 41 300 517 374 493 516 383 410 303 334 443 555 620 218 200 307 209 90 88 319 542 624
Labelled Cell Count 219 93 42 309 529 397 546 553 449 473 373 390 449 560 626 240 226 352 209 90 88 319 542 624


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.8 4.8 4.9 5.2 4.4 4.2 4.6 4.0 4.2 3.9 4.5 5.6 5.8 5.5 5.9 6.2 5.9 4.9 5.3 5.6
Std Deviation (1e-4) 0.8 0.6 0.4 1.3 1.4 1.1 1.3 1.1 1.4 1.0 1.2 3.1 0.9 1.1 1.2 1.1 1.5 1.1 1.0 1.4 1.7
Intensity Change (Log2) – – – 0.03 0.12 -0.13 -0.2 -0.07 -0.25 -0.2 -0.29 -0.09 0.22 0.28 0.2 0.31 0.38 0.29 0.02 0.15 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8896 2.1069 1.8565 1.3229 1.6837 2.0501 0.7671 1.0794 0.9357 0.7945 0.652 0.8534 -0.3145 -0.1548 -0.0958 0.021 -0.3084 -0.3478
Actin 0.0171 0.0009 0.0067 0.0042 0.0185 0.0041 0.021 0.0014 0.0121 0.0045 0.0039 0.0028 0.0107 0.0003 0.0295 0.0758 0.0008 0.0227
Bud 0.0002 0.0015 0.0002 0.0002 0.0012 0.001 0.0027 0.005 0.0032 0.0007 0.001 0.0038 0.0001 0.0001 0.0005 0.0011 0.0001 0.0026
Bud Neck 0.0036 0.0005 0.0012 0.0006 0.0019 0.0035 0.001 0.0007 0.001 0.0012 0.0018 0.003 0.0001 0.0001 0.0009 0.0019 0.0002 0.0007
Bud Periphery 0.0007 0.0009 0.0002 0.0003 0.0018 0.0011 0.0026 0.0026 0.0023 0.0007 0.0007 0.0033 0.0002 0.0001 0.0005 0.0011 0 0.0064
Bud Site 0.0018 0.0231 0.0017 0.0024 0.0132 0.0008 0.003 0.0096 0.0035 0.01 0.0044 0.0004 0.0006 0.0006 0.0111 0.0065 0.0002 0.0003
Cell Periphery 0.0004 0.0002 0.0001 0.0002 0.0003 0.0001 0.001 0.0002 0.0002 0.0001 0.0003 0.0001 0.0002 0.0002 0.0002 0.0002 0.0001 0.0002
Cytoplasm 0.4915 0.5853 0.5544 0.471 0.6179 0.5601 0.4273 0.5668 0.4806 0.5055 0.5672 0.6362 0.4381 0.6429 0.4737 0.4636 0.8834 0.7186
Cytoplasmic Foci 0.0066 0.0073 0.011 0.0418 0.0063 0.012 0.0463 0.0087 0.0206 0.0176 0.0242 0.0179 0.0222 0.004 0.0054 0.0147 0.0054 0.0062
Eisosomes 0.0009 0 0.0001 0.0004 0.0002 0 0.0002 0 0.0001 0.0001 0.0002 0 0.0005 0.0004 0.0002 0.0005 0 0.0001
Endoplasmic Reticulum 0.0062 0.003 0.0041 0.0034 0.0035 0.0033 0.0064 0.0044 0.0047 0.0066 0.0033 0.0025 0.0122 0.0047 0.0064 0.0025 0.0091 0.0087
Endosome 0.0057 0.0019 0.0037 0.0075 0.0066 0.0053 0.0213 0.0029 0.0074 0.0169 0.0177 0.0049 0.0211 0.0012 0.0067 0.006 0.0017 0.0068
Golgi 0.0018 0.0005 0.0012 0.0022 0.0008 0.0008 0.0044 0.0002 0.0022 0.0022 0.0058 0.0012 0.0059 0.0001 0.0064 0.0144 0.0002 0.002
Lipid Particles 0.0034 0.0005 0.0027 0.0078 0.0004 0.0055 0.0177 0.0002 0.0074 0.0013 0.0485 0.0039 0.0154 0.0003 0.0014 0.0106 0.0004 0.0003
Mitochondria 0.0042 0.0004 0.0012 0.0087 0.0012 0.0009 0.0118 0.0003 0.0081 0.0017 0.017 0.008 0.0028 0.0002 0.0051 0.0259 0.0001 0.0141
None 0.4347 0.3536 0.3925 0.3835 0.2869 0.3735 0.3395 0.3799 0.3972 0.4073 0.2044 0.2838 0.4499 0.3397 0.4445 0.3464 0.0922 0.1942
Nuclear Periphery 0.0038 0.0021 0.0018 0.0019 0.01 0.0029 0.018 0.0017 0.0045 0.0041 0.0035 0.0016 0.0089 0.0011 0.0016 0.001 0.0012 0.0067
Nucleolus 0.0008 0.0004 0.0003 0.0005 0.003 0.0007 0.0055 0.0002 0.0006 0.0002 0.0013 0.0003 0.0004 0.0002 0.0001 0.0002 0 0.0001
Nucleus 0.0066 0.0137 0.0098 0.0521 0.016 0.014 0.0162 0.0092 0.0114 0.0107 0.0123 0.0094 0.0019 0.0021 0.0023 0.0072 0.0028 0.0046
Peroxisomes 0.0016 0.0006 0.0039 0.0069 0.0005 0.0017 0.0403 0.0002 0.0199 0.0041 0.0695 0.0092 0.0043 0 0.0014 0.0164 0.0001 0.0001
Punctate Nuclear 0.0035 0.0026 0.0024 0.0033 0.0043 0.0078 0.0087 0.0045 0.0112 0.0023 0.0096 0.0067 0.002 0.0008 0.0006 0.0008 0.0014 0.0007
Vacuole 0.0042 0.0009 0.0007 0.001 0.0048 0.0006 0.0036 0.0011 0.0013 0.0018 0.0029 0.0009 0.0015 0.0008 0.001 0.0029 0.0006 0.0016
Vacuole Periphery 0.0006 0.0001 0.0001 0.0003 0.0007 0.0002 0.0015 0.0001 0.0006 0.0004 0.0006 0.0002 0.001 0.0001 0.0002 0.0004 0.0001 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.0313 4.5164 7.373 8.7602 9.1706 8.0791 9.5897 8.938 8.3659 9.401
Translational Efficiency 3.9433 2.7118 1.4853 1.4069 1.1506 1.4251 1.5199 1.3723 1.6602 1.6595

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Conserved peptide N-glycanase; deglycosylating enzyme that cleaves N-glycans that are attached to misfolded ERAD substrate glycoproteins prior to proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p; human ortholog NGLY1 is associated with a syndrome characterized by developmental delays, epilepsy, absence of tears and liver disease
Localization
Cell Percentages cytoplasm (50%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Png1

Png1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Png1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available