Standard name
Human Ortholog
Description Mitochondrial tyrosyl-tRNA synthetase
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.06 0.06 0.1 0.05 0.05 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.08 0.07 0.08 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0.08 0.06 0.1 0 0 0.05 0 0 0 0 0.07 0.06 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.12 0.06 0.07 0.07 0 0 0.05 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.08 0.1 0.22 0.3 0.06 0.07 0 0.06 0.05 0.06
Mitochondria 0.97 0.88 0.95 0.96 0.95 0.88 0.93 0.89 0.96 0.98 0.95 0.95 0.88 0.92 0.87 0.11 0.09 0.05 0.73 0.7 0.81 0.74 0.71 0.62
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0.05 0 0.07 0.09 0.07 0 0 0 0 0.05 0 0.05 0.05 0.06 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.26 0.1 0.17 0.06 0 0.08 0.07 0.07 0.07
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.19 0.17 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.16 0 0 0 0 0 0 0 0 0 0 0.08 0 0.08 0.12 0.22 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 4 1 1 1 0 4 1 3 0 3 4 10
Bud 3 0 7 3 10 5 9 6 9 6 7 5 3 5 4 5 3 4 1 0 0 0 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 1 0 1 0 0 1 2 4 2 0 1 4 0 0 0 1 0 1 – – – – – –
Cell Periphery 0 1 1 2 0 1 0 2 3 1 4 6 13 7 13 14 9 8 0 0 0 0 0 1
Cytoplasm 0 4 3 2 5 7 10 16 6 4 11 6 1 3 4 12 8 9 0 0 0 0 0 0
Endoplasmic Reticulum 1 0 4 1 3 1 3 2 8 3 2 0 5 10 7 10 3 3 0 0 0 1 0 3
Endosome 6 1 0 0 0 1 1 1 0 0 0 0 8 6 9 34 16 6 12 9 4 8 10 19
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 12 23 25 18 28 31 10 8 3 12 10 16
Mitochondria 168 96 108 48 97 80 168 141 172 126 206 166 172 255 266 19 12 5 128 91 93 154 152 163
Nucleus 0 0 0 0 0 2 3 3 0 2 2 1 0 0 0 5 4 4 0 0 0 0 0 1
Nuclear Periphery 6 3 3 1 5 4 13 15 12 4 9 7 3 13 7 9 6 6 0 2 0 0 5 2
Nucleolus 7 4 1 1 1 5 12 7 2 0 6 3 3 3 3 2 6 4 0 1 0 0 1 2
Peroxisomes 0 1 0 0 0 0 1 0 1 1 0 0 6 8 24 45 13 18 10 5 9 14 15 17
SpindlePole 1 0 2 0 2 1 5 3 1 2 5 2 4 0 0 32 22 6 1 1 0 0 0 0
Vac/Vac Membrane 11 17 3 0 0 3 3 3 3 5 2 5 15 11 24 21 28 11 4 4 0 4 8 10
Unique Cell Count 173 109 114 50 102 91 181 158 180 129 217 174 196 277 306 172 129 103 175 130 115 208 214 262
Labelled Cell Count 204 128 133 58 123 111 230 203 219 154 255 205 249 345 387 228 158 120 175 130 115 208 214 262


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 6.5 4.6 4.5 4.2 4.4 3.4 4.2 3.8 3.7 3.8 3.5 6.6 6.4 6.5 6.8 7.9 7.7 5.9 6.0 6.8
Std Deviation (1e-4) 0.9 1.0 0.9 0.7 0.9 1.7 0.8 1.6 0.9 1.3 1.2 1.2 1.3 1.2 1.1 2.1 2.0 1.5 1.2 1.4 1.5
Intensity Change (Log2) – – – -0.04 -0.12 -0.07 -0.42 -0.14 -0.28 -0.3 -0.29 -0.41 0.51 0.48 0.5 0.57 0.79 0.75 0.37 0.39 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – 0 0 – – – – 0 0 0 0 0 -0.8
Bud Neck – – – 0 0 – – – – 0 0 0 0 0 0
Bud Site – – – 0 0 – – – – 0 0 0 0 0 0
Cell Periphery – – – 0 0 – – – – 2.4 0 0 2.7 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – 0 0 – – – – 0 0 0 0.9 0 0
Endosome – – – 0 0 – – – – 0 0 0 5.1 3.9 0
Golgi – – – 0 0 – – – – 0 3.2 3.1 3.6 5.3 6.3
Mitochondria – – – 0 0 – – – – -2.0 -0.9 -2.3 -13.9 -13.3 -13.2
Nuclear Periphery – – – 0 0 – – – – 0 0 0 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – 0 0 – – – – 0 0 0 0 0 0
Peroxisomes – – – 0 0 – – – – 0 0 3.1 5.9 3.5 4.7
SpindlePole – – – 0 0 – – – – 0 0 0 4.3 4.0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – 0 0 – – – – 0 0 0 0 0 0
Cytoplasm – – – 1.2 2.4 – – – – 0 0 0 1.6 1.3 2.0
Nucleus – – – 0 0 – – – – 0 0 0 0 0 0
Vacuole – – – 0 0 – – – – 1.8 0 1.9 2.9 4.4 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.9444 4.7539 4.4859 4.1629 4.1388 4.4568 3.8081 3.7424 3.7082 4.1208 3.0328 3.8532 5.3425 5.5541 4.8063 4.8784 4.722 5.2228
Actin 0.0676 0.0203 0.0533 0.0022 0.0158 0.0065 0.0356 0.0391 0.0402 0.0115 0.0179 0.0158 0.04 0.0109 0.0237 0.0467 0.0023 0.0064
Bud 0.0008 0.0004 0.0007 0.0002 0.0002 0.0004 0.0006 0.0014 0.0004 0.0001 0.0002 0.0003 0.0006 0.0011 0.0007 0.0005 0.0002 0.0007
Bud Neck 0.0019 0.0005 0.0071 0.0001 0.0008 0.0004 0.0007 0.0009 0.0006 0.0005 0.0003 0.0004 0.0047 0.0005 0.0028 0.0004 0.0002 0.0033
Bud Periphery 0.0023 0.0008 0.0013 0.0005 0.0004 0.0012 0.0011 0.0018 0.001 0.0003 0.0003 0.0008 0.0012 0.0036 0.001 0.0017 0.0009 0.0032
Bud Site 0.0027 0.0106 0.0024 0.0002 0.0008 0.0004 0.007 0.0255 0.0022 0.0003 0.0006 0.0004 0.0041 0.0116 0.0071 0.0008 0.0003 0.0008
Cell Periphery 0.0017 0.0005 0.0007 0.0001 0.0003 0.0003 0.0008 0.0005 0.0039 0.0001 0.0001 0.0012 0.001 0.0015 0.0008 0.0004 0.0002 0.001
Cytoplasm 0.0015 0.0006 0.0018 0 0.0006 0.0001 0.0015 0.0008 0.0022 0.0002 0.0002 0.0019 0.0018 0.0008 0.0023 0.0004 0.0001 0.0006
Cytoplasmic Foci 0.0137 0.0074 0.01 0.005 0.0175 0.0042 0.027 0.0231 0.02 0.0097 0.0174 0.0147 0.0292 0.0122 0.0155 0.0288 0.0051 0.0054
Eisosomes 0.0042 0.0007 0.0021 0.0001 0.0004 0.0003 0.0012 0.0015 0.0013 0.0002 0.0001 0.0009 0.0018 0.0025 0.0006 0.0003 0.0002 0.0008
Endoplasmic Reticulum 0.0032 0.0008 0.0113 0.0001 0.0005 0.0004 0.0011 0.0002 0.0003 0.0001 0.0001 0.0006 0.0053 0.0003 0.0016 0.0002 0.0001 0.0005
Endosome 0.0578 0.0286 0.02 0.0091 0.0181 0.0104 0.0369 0.0307 0.0293 0.0086 0.045 0.02 0.0456 0.0496 0.0266 0.0092 0.0103 0.0252
Golgi 0.0614 0.0526 0.0282 0.0216 0.0826 0.0193 0.0449 0.042 0.0395 0.0265 0.0797 0.0374 0.0405 0.0672 0.0184 0.0429 0.0233 0.0273
Lipid Particles 0.0424 0.0205 0.027 0.0172 0.0401 0.0238 0.0353 0.0319 0.0128 0.0171 0.005 0.0167 0.0412 0.0241 0.0218 0.0053 0.0168 0.0134
Mitochondria 0.6771 0.8026 0.7986 0.921 0.7703 0.8996 0.7353 0.7408 0.8084 0.8942 0.775 0.8351 0.6674 0.7487 0.8249 0.7634 0.8464 0.8662
None 0.002 0.0061 0.0023 0.0001 0.0011 0.0001 0.0048 0.0002 0.0004 0.0005 0.0001 0.0033 0.0128 0.0133 0.0008 0.0005 0.0001 0.0002
Nuclear Periphery 0.0111 0.0039 0.0029 0.0002 0.0039 0.0043 0.001 0.0001 0.0001 0.0001 0 0.0002 0.0102 0.0002 0.0017 0.0001 0 0.0006
Nucleolus 0.0005 0.001 0.0007 0.0001 0.0007 0.0002 0.0012 0.0004 0.0001 0 0 0.0001 0.0021 0.001 0.0003 0.0001 0.0003 0.0016
Nucleus 0.0025 0.0004 0.001 0.0001 0.0004 0.0001 0.0006 0.0002 0.0001 0 0 0.0001 0.0013 0.0006 0.0004 0.0001 0.0001 0.0005
Peroxisomes 0.0365 0.0299 0.0229 0.0185 0.0216 0.0211 0.0484 0.0554 0.0351 0.0291 0.0532 0.0473 0.0713 0.0323 0.0425 0.0964 0.0885 0.0271
Punctate Nuclear 0.0027 0.0018 0.0019 0 0.0191 0.0001 0.0082 0.0001 0.0001 0.0002 0.0001 0.0005 0.0103 0.0002 0.0009 0.0001 0 0.0001
Vacuole 0.0025 0.0035 0.0017 0.0008 0.0012 0.0016 0.0034 0.0025 0.0009 0.0002 0.0014 0.0009 0.0025 0.0084 0.0027 0.0008 0.0024 0.007
Vacuole Periphery 0.0039 0.0064 0.0022 0.0028 0.0035 0.0053 0.0035 0.001 0.0011 0.0005 0.0032 0.0014 0.005 0.0094 0.0027 0.001 0.0018 0.0081

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.8335 5.3354 7.4233 11.3274 3.3855 5.9651 11.4635 15.2466 12.5912 13.1287
Translational Efficiency 0.9699 1.0362 0.9267 0.8622 2.0096 1.5778 1.0314 0.8002 0.9594 0.8225

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial tyrosyl-tRNA synthetase
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Msy1

Msy1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msy1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available