Standard name | |
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Human Ortholog | |
Description | Mitochondrial aspartyl-tRNA synthetase; required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 0 | 6 | 2 | 0 | 0 | – | 0 | 1 | 1 | 0 | 4 | 8 | 0 | 2 | 2 | 1 | 3 | 3 | 2 | 0 | 3 | 7 |
Bud | 2 | 0 | 1 | 8 | 7 | 7 | 11 | – | 9 | 12 | 9 | 10 | 1 | 5 | 7 | 6 | 10 | 6 | 0 | 1 | 0 | 0 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 2 | – | 5 | 3 | 6 | 3 | 0 | 1 | 0 | 2 | 1 | 4 | – | – | – | – | – | – |
Cell Periphery | 0 | 5 | 1 | 5 | 7 | 2 | 5 | – | 2 | 5 | 3 | 1 | 3 | 7 | 5 | 12 | 11 | 7 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 3 | 8 | 1 | 15 | 12 | 8 | 15 | – | 5 | 6 | 1 | 5 | 3 | 10 | 16 | 7 | 15 | 15 | 0 | 1 | 0 | 0 | 0 | 5 |
Endoplasmic Reticulum | 1 | 0 | 0 | 2 | 4 | 0 | 2 | – | 0 | 5 | 1 | 3 | 1 | 3 | 1 | 2 | 7 | 7 | 0 | 0 | 1 | 0 | 0 | 1 |
Endosome | 6 | 17 | 11 | 22 | 51 | 16 | 37 | – | 9 | 11 | 0 | 5 | 17 | 16 | 46 | 37 | 46 | 40 | 13 | 18 | 12 | 6 | 18 | 13 |
Golgi | 0 | 2 | 1 | 8 | 24 | 0 | 3 | – | 1 | 0 | 0 | 3 | 12 | 14 | 19 | 23 | 24 | 17 | 4 | 6 | 1 | 1 | 4 | 9 |
Mitochondria | 107 | 85 | 15 | 125 | 180 | 177 | 276 | – | 252 | 382 | 247 | 297 | 45 | 44 | 41 | 28 | 37 | 24 | 84 | 92 | 57 | 38 | 132 | 152 |
Nucleus | 0 | 1 | 0 | 1 | 2 | 12 | 15 | – | 4 | 6 | 3 | 8 | 1 | 2 | 0 | 2 | 7 | 5 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 2 | 3 | 11 | – | 11 | 33 | 18 | 15 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 7 | 1 | 1 | 0 | 2 | 12 | 11 | – | 8 | 18 | 11 | 11 | 1 | 0 | 1 | 2 | 6 | 4 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 4 | 13 | 2 | 24 | 30 | 7 | 16 | – | 3 | 3 | 2 | 2 | 5 | 8 | 40 | 48 | 53 | 42 | 7 | 7 | 7 | 0 | 6 | 8 |
SpindlePole | 12 | 13 | 15 | 25 | 54 | 17 | 29 | – | 17 | 31 | 7 | 13 | 10 | 8 | 39 | 62 | 50 | 45 | 3 | 3 | 5 | 0 | 1 | 4 |
Vac/Vac Membrane | 5 | 26 | 11 | 4 | 49 | 21 | 48 | – | 29 | 33 | 11 | 18 | 7 | 7 | 18 | 43 | 51 | 37 | 0 | 3 | 6 | 1 | 13 | 20 |
Unique Cell Count | 117 | 131 | 46 | 187 | 343 | 236 | 374 | 281 | 451 | 258 | 324 | 83 | 103 | 165 | 210 | 234 | 183 | 126 | 142 | 99 | 50 | 190 | 236 | |
Labelled Cell Count | 148 | 174 | 59 | 245 | 426 | 284 | 481 | 355 | 549 | 320 | 394 | 111 | 133 | 233 | 279 | 323 | 255 | 126 | 142 | 99 | 50 | 190 | 236 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 5.9 | 6.1 | 6.3 | 6.9 | 5.5 | 5.9 | – | 5.3 | 5.4 | 5.0 | 5.3 | 6.4 | 6.5 | 6.4 | 7.6 | 7.9 | 7.5 | – | 6.1 | 6.2 |
Std Deviation (1e-4) | 0.6 | 0.9 | 1.0 | 1.7 | 2.3 | 1.3 | 1.3 | – | 0.9 | 1.1 | 1.2 | 1.2 | 1.1 | 0.9 | 1.1 | 1.5 | 2.1 | 2.0 | – | 1.2 | 1.2 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.18 | -0.15 | -0.04 | – | -0.21 | -0.17 | -0.27 | -0.19 | 0.08 | 0.11 | 0.08 | 0.33 | 0.39 | 0.31 | – | 0.01 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -2.1 | -1.6 | 0 | -2.8 | – | 0 | 0 | 0 | 0 | -0.5 | -1.2 | 0.5 | -1.0 | -0.7 | -0.3 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.2 | 2.5 | 5.6 | 5.7 | – | 9.3 | 8.4 | 11.4 | 10.3 | 2.4 | 1.2 | -1.1 | -3.2 | -2.7 | -3.1 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.6 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 2.9 | 2.9 | 2.9 |
SpindlePole | -3.1 | -2.8 | -5.0 | 0 | – | 0 | 0 | 0 | 0 | -2.8 | -3.9 | -1.2 | -0.4 | -1.7 | -1.1 |
Vacuole | 0 | -1.7 | -2.9 | -2.0 | – | -2.6 | 0 | 0 | 0 | -2.4 | -3.0 | -2.3 | -0.5 | -0.3 | -0.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0324 | 0.0169 | 0.0361 | 0.0033 | 0.0006 | 0.0364 | – | – | – | – | – | – | 0.0392 | 0.01 | 0.0116 | 0.0186 | 0.0101 | 0.0108 |
Bud | 0.0039 | 0.0069 | 0.004 | 0.0023 | 0.0001 | 0.0014 | – | – | – | – | – | – | 0.0008 | 0.0064 | 0.0024 | 0.0016 | 0.0077 | 0.0007 |
Bud Neck | 0.0015 | 0.0015 | 0.0022 | 0.0267 | 0.0001 | 0.0039 | – | – | – | – | – | – | 0.0044 | 0.002 | 0.0014 | 0.0011 | 0.0015 | 0.0096 |
Bud Periphery | 0.005 | 0.0112 | 0.0114 | 0.0055 | 0.0002 | 0.0057 | – | – | – | – | – | – | 0.0016 | 0.0047 | 0.0068 | 0.0035 | 0.0241 | 0.0016 |
Bud Site | 0.0088 | 0.0403 | 0.0102 | 0.0029 | 0.0001 | 0.0026 | – | – | – | – | – | – | 0.0041 | 0.0156 | 0.0139 | 0.003 | 0.0021 | 0.0013 |
Cell Periphery | 0.0011 | 0.0013 | 0.0022 | 0.0019 | 0.0002 | 0.0041 | – | – | – | – | – | – | 0.0007 | 0.0012 | 0.0013 | 0.0004 | 0.0004 | 0.0005 |
Cytoplasm | 0.0131 | 0.0113 | 0.0073 | 0.0026 | 0.0001 | 0.0188 | – | – | – | – | – | – | 0.0153 | 0.0258 | 0.0168 | 0.0035 | 0.0045 | 0.0121 |
Cytoplasmic Foci | 0.0582 | 0.0613 | 0.0467 | 0.0253 | 0.0024 | 0.0321 | – | – | – | – | – | – | 0.0577 | 0.061 | 0.0416 | 0.0389 | 0.053 | 0.0427 |
Eisosomes | 0.0006 | 0.0005 | 0.0015 | 0.0016 | 0.0001 | 0.0009 | – | – | – | – | – | – | 0.0006 | 0.0002 | 0.0005 | 0.0004 | 0.0003 | 0.0003 |
Endoplasmic Reticulum | 0.0073 | 0.003 | 0.0027 | 0.0005 | 0.0003 | 0.0036 | – | – | – | – | – | – | 0.0071 | 0.0034 | 0.002 | 0.0023 | 0.0012 | 0.0029 |
Endosome | 0.1145 | 0.0698 | 0.066 | 0.0728 | 0.032 | 0.0635 | – | – | – | – | – | – | 0.1691 | 0.1782 | 0.1068 | 0.1026 | 0.1291 | 0.0966 |
Golgi | 0.0948 | 0.0813 | 0.0524 | 0.0561 | 0.0146 | 0.075 | – | – | – | – | – | – | 0.0662 | 0.0822 | 0.0476 | 0.0377 | 0.0646 | 0.055 |
Lipid Particles | 0.04 | 0.0424 | 0.0322 | 0.0162 | 0.0237 | 0.0332 | – | – | – | – | – | – | 0.051 | 0.0234 | 0.0284 | 0.0114 | 0.0207 | 0.0289 |
Mitochondria | 0.4517 | 0.5436 | 0.6117 | 0.6378 | 0.8823 | 0.637 | – | – | – | – | – | – | 0.4353 | 0.4766 | 0.6415 | 0.7026 | 0.6257 | 0.6674 |
None | 0.0122 | 0.0046 | 0.0394 | 0.0678 | 0.0001 | 0.01 | – | – | – | – | – | – | 0.0293 | 0.0199 | 0.0064 | 0.0014 | 0.0028 | 0.008 |
Nuclear Periphery | 0.0095 | 0.0031 | 0.0009 | 0.0007 | 0.0008 | 0.0015 | – | – | – | – | – | – | 0.0113 | 0.002 | 0.0047 | 0.0017 | 0.0013 | 0.0037 |
Nucleolus | 0.0056 | 0.0011 | 0.0013 | 0.0053 | 0.0003 | 0.0005 | – | – | – | – | – | – | 0.002 | 0.0043 | 0.0009 | 0.0004 | 0.0004 | 0.0012 |
Nucleus | 0.0064 | 0.0038 | 0.0009 | 0.0024 | 0.0002 | 0.0009 | – | – | – | – | – | – | 0.004 | 0.002 | 0.0072 | 0.0005 | 0.0007 | 0.0011 |
Peroxisomes | 0.0966 | 0.0725 | 0.0468 | 0.0336 | 0.0133 | 0.0597 | – | – | – | – | – | – | 0.0606 | 0.0462 | 0.0335 | 0.0427 | 0.0346 | 0.0338 |
Punctate Nuclear | 0.0044 | 0.001 | 0.0007 | 0.0024 | 0 | 0.0008 | – | – | – | – | – | – | 0.0106 | 0.0008 | 0.0012 | 0.0006 | 0.0007 | 0.0026 |
Vacuole | 0.0179 | 0.0165 | 0.0132 | 0.0253 | 0.0089 | 0.0052 | – | – | – | – | – | – | 0.0197 | 0.0255 | 0.0156 | 0.0136 | 0.0085 | 0.0119 |
Vacuole Periphery | 0.0144 | 0.0061 | 0.0104 | 0.007 | 0.0198 | 0.0031 | – | – | – | – | – | – | 0.0094 | 0.0087 | 0.0078 | 0.0117 | 0.0059 | 0.0073 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.0927 | 10.9764 | 6.2938 | 9.878 | 12.3017 | 7.8094 | 12.3594 | 12.4275 | 14.8402 | 13.9164 |
Translational Efficiency | 0.5775 | 0.4192 | 0.613 | 0.548 | 0.3502 | 0.8165 | 0.5496 | 0.6152 | 0.4957 | 0.4968 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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