Standard name | |
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Human Ortholog | |
Description | UbiB family protein; contains transmembrane domain and mitochondrial targeting sequence; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 7 | 2 | 8 | 16 | 0 | 5 | 7 |
Bud | 3 | 6 | 12 | 7 | 10 | 3 | 16 | 24 | 9 | 11 | 4 | 2 | 1 | 5 | 0 | 7 | 4 | 1 | 1 | 2 | 0 | 1 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 4 | 1 | 3 | 6 |
Bud Site | 1 | 0 | 1 | 1 | 0 | 5 | 4 | 4 | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | 4 | 3 | 1 | 4 | 2 | 1 | 1 | 2 | 0 | 1 | 6 | 3 | 1 | 14 | 12 | 6 | 0 | 0 | 1 | 0 | 1 | 1 |
Cytoplasm | 20 | 27 | 34 | 18 | 36 | 30 | 50 | 62 | 22 | 43 | 8 | 7 | 48 | 53 | 89 | 109 | 57 | 41 | 1 | 10 | 1 | 1 | 9 | 3 |
Endoplasmic Reticulum | 0 | 7 | 3 | 3 | 0 | 4 | 2 | 2 | 3 | 10 | 5 | 0 | 2 | 2 | 3 | 20 | 13 | 7 | 3 | 3 | 2 | 1 | 1 | 1 |
Endosome | 5 | 7 | 7 | 7 | 38 | 4 | 6 | 13 | 0 | 0 | 0 | 0 | 16 | 15 | 30 | 59 | 33 | 20 | 12 | 13 | 26 | 3 | 8 | 8 |
Golgi | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 9 | 2 | 1 | 4 |
Mitochondria | 119 | 106 | 170 | 132 | 180 | 328 | 362 | 370 | 300 | 402 | 51 | 48 | 75 | 34 | 25 | 44 | 19 | 11 | 105 | 111 | 196 | 55 | 93 | 90 |
Nucleus | 6 | 1 | 1 | 0 | 1 | 8 | 8 | 15 | 7 | 4 | 1 | 0 | 0 | 1 | 0 | 2 | 7 | 2 | 0 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 2 | 2 | 10 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 0 | 0 | 0 |
Nucleolus | 7 | 0 | 0 | 1 | 0 | 2 | 3 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 |
Peroxisomes | 1 | 2 | 23 | 6 | 17 | 5 | 5 | 9 | 1 | 0 | 1 | 0 | 8 | 4 | 12 | 66 | 15 | 13 | 2 | 5 | 21 | 2 | 2 | 4 |
SpindlePole | 0 | 2 | 11 | 2 | 16 | 13 | 32 | 38 | 14 | 16 | 2 | 0 | 4 | 0 | 1 | 26 | 23 | 17 | 1 | 1 | 3 | 0 | 3 | 2 |
Vac/Vac Membrane | 8 | 18 | 5 | 3 | 20 | 12 | 17 | 21 | 10 | 13 | 1 | 1 | 13 | 19 | 22 | 47 | 34 | 16 | 9 | 16 | 5 | 1 | 9 | 17 |
Unique Cell Count | 136 | 147 | 231 | 151 | 246 | 372 | 419 | 444 | 317 | 443 | 62 | 56 | 137 | 121 | 161 | 298 | 189 | 124 | 152 | 184 | 298 | 79 | 148 | 158 |
Labelled Cell Count | 170 | 182 | 275 | 183 | 320 | 423 | 509 | 566 | 376 | 518 | 74 | 60 | 174 | 136 | 183 | 396 | 222 | 142 | 152 | 184 | 298 | 79 | 148 | 158 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 4.7 | 4.6 | 4.2 | 4.1 | 3.8 | 3.2 | 3.7 | 3.4 | 2.9 | 3.6 | 3.4 | 4.9 | 4.9 | 4.9 | 5.6 | 6.5 | 6.3 | 4.4 | 4.9 | 5.8 |
Std Deviation (1e-4) | 0.5 | 0.7 | 1.2 | 0.9 | 1.2 | 1.2 | 0.9 | 1.2 | 0.9 | 0.7 | 1.5 | 1.3 | 1.0 | 1.3 | 1.4 | 1.7 | 1.8 | 1.2 | 1.5 | 1.8 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.15 | -0.28 | -0.52 | -0.31 | -0.43 | -0.64 | -0.35 | -0.42 | 0.11 | 0.08 | 0.1 | 0.29 | 0.51 | 0.46 | -0.07 | 0.1 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 5.2 | 5.6 | 4.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8529 | 1.0118 | 0.5438 | 0.9635 | 0.2925 | 0.7866 | 1.8052 | 2.1708 | 2.1774 | 1.4882 | 2.2336 | 1.8077 | 3.5648 | 3.3797 | 3.2341 | 3.2775 | 3.1271 | 3.4774 |
Actin | 0.0879 | 0.0283 | 0.0305 | 0.0168 | 0.182 | 0.0224 | 0.056 | 0.046 | 0.0203 | 0.0472 | 0.0406 | 0.0074 | 0.0382 | 0.0111 | 0.0153 | 0.0151 | 0.0459 | 0.0178 |
Bud | 0.0025 | 0.0063 | 0.0096 | 0.0018 | 0.0018 | 0.0027 | 0.002 | 0.0038 | 0.0052 | 0.0091 | 0.0048 | 0.0028 | 0.0018 | 0.0053 | 0.0055 | 0.0105 | 0.0027 | 0.0016 |
Bud Neck | 0.0017 | 0.0044 | 0.0046 | 0.0013 | 0.006 | 0.0236 | 0.0064 | 0.0038 | 0.0013 | 0.002 | 0.0011 | 0.0034 | 0.0025 | 0.0051 | 0.0055 | 0.0095 | 0.0014 | 0.0138 |
Bud Periphery | 0.0037 | 0.0094 | 0.0389 | 0.0067 | 0.0022 | 0.0124 | 0.005 | 0.0104 | 0.0141 | 0.0331 | 0.007 | 0.0079 | 0.0032 | 0.0098 | 0.022 | 0.0392 | 0.0058 | 0.0052 |
Bud Site | 0.0073 | 0.1246 | 0.0326 | 0.003 | 0.0041 | 0.0041 | 0.0516 | 0.0689 | 0.0112 | 0.01 | 0.0117 | 0.0009 | 0.0152 | 0.0475 | 0.0404 | 0.0098 | 0.0097 | 0.003 |
Cell Periphery | 0.0015 | 0.0092 | 0.0061 | 0.051 | 0.0002 | 0.0114 | 0.0052 | 0.0075 | 0.0042 | 0.0073 | 0.0005 | 0.001 | 0.0027 | 0.0071 | 0.0071 | 0.0117 | 0.0015 | 0.0039 |
Cytoplasm | 0.0138 | 0.067 | 0.0612 | 0.1197 | 0.0201 | 0.0713 | 0.0886 | 0.0886 | 0.0785 | 0.1058 | 0.0232 | 0.0549 | 0.0662 | 0.1191 | 0.0651 | 0.0679 | 0.0207 | 0.0617 |
Cytoplasmic Foci | 0.1588 | 0.0455 | 0.1609 | 0.0343 | 0.1907 | 0.1063 | 0.0781 | 0.1033 | 0.0636 | 0.0985 | 0.1394 | 0.0775 | 0.1046 | 0.1101 | 0.0897 | 0.0675 | 0.0921 | 0.0797 |
Eisosomes | 0.0068 | 0.0021 | 0.001 | 0.0022 | 0.0007 | 0.0018 | 0.0015 | 0.0036 | 0.0026 | 0.0016 | 0.0003 | 0.0006 | 0.0015 | 0.0014 | 0.002 | 0.0018 | 0.0003 | 0.0021 |
Endoplasmic Reticulum | 0.0057 | 0.0202 | 0.0045 | 0.0431 | 0.0008 | 0.0235 | 0.0673 | 0.0119 | 0.0073 | 0.0056 | 0.001 | 0.0039 | 0.0144 | 0.0042 | 0.0037 | 0.0058 | 0.0027 | 0.0146 |
Endosome | 0.1446 | 0.1222 | 0.1108 | 0.14 | 0.0485 | 0.0894 | 0.1478 | 0.1006 | 0.0812 | 0.0589 | 0.0816 | 0.091 | 0.1534 | 0.1407 | 0.1405 | 0.1598 | 0.1761 | 0.1387 |
Golgi | 0.0581 | 0.0337 | 0.0672 | 0.0735 | 0.0453 | 0.0749 | 0.0467 | 0.0591 | 0.0557 | 0.0428 | 0.0571 | 0.0558 | 0.0831 | 0.0657 | 0.0726 | 0.0528 | 0.0766 | 0.0871 |
Lipid Particles | 0.0465 | 0.0415 | 0.0196 | 0.0159 | 0.0929 | 0.0309 | 0.0421 | 0.0125 | 0.0196 | 0.0186 | 0.0107 | 0.0147 | 0.0397 | 0.0216 | 0.0178 | 0.0099 | 0.0097 | 0.0181 |
Mitochondria | 0.154 | 0.2561 | 0.3248 | 0.4444 | 0.0188 | 0.3057 | 0.1741 | 0.2428 | 0.3724 | 0.4526 | 0.2931 | 0.4622 | 0.322 | 0.3297 | 0.4016 | 0.4244 | 0.4617 | 0.4077 |
None | 0.0459 | 0.1709 | 0.0695 | 0.0163 | 0.1609 | 0.1418 | 0.1079 | 0.1649 | 0.2025 | 0.028 | 0.2579 | 0.165 | 0.0869 | 0.0842 | 0.0575 | 0.069 | 0.0541 | 0.0931 |
Nuclear Periphery | 0.0399 | 0.0051 | 0.0007 | 0.0029 | 0.0021 | 0.0077 | 0.0179 | 0.0015 | 0.0022 | 0.0018 | 0.0008 | 0.001 | 0.0085 | 0.0008 | 0.0008 | 0.0011 | 0.0006 | 0.0033 |
Nucleolus | 0.0121 | 0.0037 | 0.0009 | 0.0005 | 0.0044 | 0.0016 | 0.0018 | 0.0008 | 0.0011 | 0.0006 | 0.0005 | 0.0005 | 0.0006 | 0.0014 | 0.001 | 0.0009 | 0.0004 | 0.0007 |
Nucleus | 0.0557 | 0.0045 | 0.0012 | 0.0015 | 0.0022 | 0.0017 | 0.0038 | 0.0013 | 0.0034 | 0.0014 | 0.0006 | 0.0008 | 0.0021 | 0.002 | 0.001 | 0.0014 | 0.0007 | 0.0011 |
Peroxisomes | 0.0434 | 0.0114 | 0.0347 | 0.0094 | 0.1773 | 0.0427 | 0.0465 | 0.041 | 0.037 | 0.0503 | 0.0589 | 0.037 | 0.0351 | 0.0172 | 0.0299 | 0.0178 | 0.0156 | 0.0318 |
Punctate Nuclear | 0.0107 | 0.0038 | 0.0022 | 0.0006 | 0.0364 | 0.0013 | 0.0187 | 0.0012 | 0.0007 | 0.0034 | 0.0008 | 0.0011 | 0.0015 | 0.002 | 0.0041 | 0.001 | 0.0006 | 0.0006 |
Vacuole | 0.0636 | 0.0238 | 0.0128 | 0.0086 | 0.0017 | 0.0165 | 0.0234 | 0.0217 | 0.0106 | 0.0164 | 0.0048 | 0.0067 | 0.0118 | 0.0105 | 0.0112 | 0.0161 | 0.0145 | 0.0097 |
Vacuole Periphery | 0.0357 | 0.0061 | 0.0057 | 0.0066 | 0.0008 | 0.0064 | 0.0076 | 0.0046 | 0.0051 | 0.0049 | 0.0035 | 0.0041 | 0.0051 | 0.0035 | 0.0054 | 0.0069 | 0.0066 | 0.0046 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.8692 | 4.4972 | 4.4494 | 6.1769 | 2.1742 | 5.0279 | 7.9574 | 6.8199 | 8.0989 | 7.3105 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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