Standard name
Human Ortholog
Description Rho GTPase activating protein (RhoGAP); involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.18 0.18 0.11 0.07 0.07 0.05 0.05 0 0 0 0 0 0.05 0 0 0.14 0.11 0.14 0.13 0.1 0.09 0.12 0.1 0.09
Bud 0 0 0 0 0.07 0 0.05 0.05 0.05 0.06 0 0.06 0 0 0.05 0 0 0 0.1 0.07 0.05 0.16 0.06 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.16 0.12 0.1 0 0
Bud Site 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0.05 0.05 0 0 0.07 0.07 0.15 0.14 0.14 0.12 0 0 0 0 0 0
Cytoplasm 0.71 0.73 0.78 0.81 0.74 0.73 0.66 0.66 0.64 0.61 0.62 0.53 0.92 0.95 0.94 0.65 0.64 0.68 0.47 0.51 0.47 0.52 0.58 0.65
Endoplasmic Reticulum 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0.07 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.2 0.15 0.29 0.35 0.39 0.45 0.51 0.52 0.59 0 0 0 0 0 0.05 0 0 0.13 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.12 0.13 0 0 0.05 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 13 46 39 18 25 21 20 13 13 7 3 1 9 10 6 25 13 21 15 30 38 15 26 23
Bud 1 7 11 4 27 12 21 32 20 23 11 24 4 4 13 1 3 0 12 20 20 20 16 11
Bud Neck 0 0 5 1 3 1 3 2 2 2 3 2 0 0 0 2 3 2 19 48 50 13 9 6
Bud Site 5 7 15 2 11 5 10 11 6 8 2 7 0 0 0 4 2 6 – – – – – –
Cell Periphery 1 10 17 11 2 11 10 8 23 21 12 18 13 18 44 25 17 18 2 3 6 1 3 3
Cytoplasm 52 185 266 211 269 288 273 388 277 250 188 216 175 245 268 118 76 100 58 156 196 69 151 167
Endoplasmic Reticulum 0 18 9 6 4 14 7 5 9 8 1 2 5 2 5 8 4 12 1 4 13 2 18 13
Endosome 1 3 1 0 4 3 1 0 0 0 0 1 2 2 0 6 4 3 4 12 12 1 3 7
Golgi 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 2 0 3 0 2 1
Mitochondria 2 5 11 53 55 114 145 225 198 209 159 242 0 0 3 7 3 7 3 8 53 1 10 7
Nucleus 0 1 1 0 0 3 3 4 3 0 4 0 2 1 1 3 1 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 1 0 0 1 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 1 0 0 5 2 2 0 1 0 2 3 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 7 8 5 0 11 1 4 3 2 0 1 4 0 1 0 4 3 2 0 0 0 0 0 0
SpindlePole 5 4 16 1 10 3 9 5 6 8 0 7 1 0 0 11 9 10 1 4 5 0 0 3
Vac/Vac Membrane 2 3 0 0 7 0 12 27 0 4 1 8 0 0 0 21 16 6 2 14 11 4 12 6
Unique Cell Count 73 254 342 261 362 396 412 584 436 412 305 409 191 257 286 182 119 146 125 310 419 132 259 258
Labelled Cell Count 90 298 398 308 433 478 521 724 560 540 387 537 211 283 340 236 156 187 125 310 419 132 259 258


Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 6.4 4.9 4.4 4.9 4.4 4.3 4.2 3.7 3.7 3.8 3.6 6.9 6.8 5.9 8.2 9.2 9.2 7.3 7.6 7.5
Std Deviation (1e-4) 1.1 0.7 0.6 0.5 1.0 0.8 0.9 1.0 0.7 0.7 1.1 1.1 1.1 1.1 1.0 1.3 1.9 2.2 1.1 1.5 1.0
Intensity Change (Log2) – – – -0.14 0.0 -0.16 -0.19 -0.22 -0.39 -0.4 -0.36 -0.42 0.51 0.48 0.28 0.75 0.92 0.92 0.58 0.63 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -2.1 0 0 0 0 0 0 0 -2.6 -3.3 -4.5 0.8 -0.1 0.9
Bud 0 2.5 0 0 0 0 0 0 0 -0.8 -1.3 0.9 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 -0.9 0 0 0 0 0 0 0 -2.9 -3.4 -3.6 -1.3 0 -0.1
Cell Periphery 0 -3.6 0 0 0 0 0 0 0 0.9 1.0 4.4 3.5 3.3 2.9
Cytoplasm 0.9 -1.1 -1.6 -3.5 -3.7 -4.3 -5.0 -4.5 -7.1 4.1 6.0 5.6 -3.2 -3.0 -2.2
Endoplasmic Reticulum 0 -1.5 0 0 0 0 0 0 0 0 0 -0.7 1.1 0 2.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 5.4 0 0 0 0 0 0 0 0 0 -1.8 0.4 0 0.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1.4 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 -1.3 0 0 0 0 0 0 0 -2.6 -3.5 -3.7 0.7 1.2 1.0
Vacuole 0 0 0 3.2 4.0 0 0 0 0 0 0 0 6.4 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2719 3.0495 2.7076 3.1808 2.0267 2.8846 5.7426 5.6257 5.6413 4.8344 6.3073 5.8822 5.6185 5.842 5.2749 6.079 5.4756 5.1232
Actin 0.1961 0.0204 0.0653 0.029 0.0333 0.0353 0.0537 0.0204 0.0512 0.0546 0.017 0.0413 0.0391 0.004 0.0335 0.0408 0.0242 0.0288
Bud 0.002 0.0033 0.0342 0.0106 0.0024 0.0014 0.0071 0.0252 0.0275 0.0381 0.0075 0.0071 0.0063 0.0134 0.0128 0.0089 0.0018 0.0021
Bud Neck 0.072 0.0026 0.0087 0.0897 0.0015 0.5346 0.0407 0.0079 0.009 0.0009 0.0041 0.426 0.0595 0.0065 0.0031 0.0023 0.0015 0.4238
Bud Periphery 0.0031 0.003 0.0515 0.0122 0.0018 0.0023 0.0126 0.0121 0.0453 0.0947 0.0149 0.0052 0.0105 0.0157 0.0111 0.0112 0.0024 0.0027
Bud Site 0.0805 0.192 0.0417 0.0541 0.0015 0.0027 0.0828 0.1165 0.0452 0.0113 0.01 0.0112 0.1113 0.2883 0.0482 0.0168 0.0016 0.0168
Cell Periphery 0.0049 0.005 0.0064 0.0024 0.0007 0.0022 0.0054 0.0037 0.0041 0.0009 0.0041 0.0014 0.0052 0.003 0.0018 0.0021 0.0006 0.0018
Cytoplasm 0.3941 0.7021 0.6857 0.6078 0.8723 0.3668 0.4958 0.6566 0.6445 0.5767 0.7243 0.3801 0.5262 0.5157 0.7362 0.7837 0.8728 0.4602
Cytoplasmic Foci 0.0539 0.0115 0.0141 0.0261 0.0447 0.0233 0.0485 0.0135 0.0305 0.0222 0.0888 0.0332 0.0325 0.0145 0.047 0.0263 0.0352 0.0273
Eisosomes 0.0026 0.0002 0.0004 0.0011 0 0.0001 0.0009 0.0004 0.0006 0.0002 0.0002 0.0005 0.0006 0.0004 0.0005 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0126 0.0041 0.0089 0.0078 0.0043 0.0022 0.0213 0.0058 0.0089 0.0071 0.004 0.009 0.012 0.0034 0.0039 0.0055 0.0038 0.002
Endosome 0.0422 0.009 0.0168 0.0612 0.0079 0.0064 0.0384 0.0078 0.0261 0.0551 0.0231 0.0186 0.0305 0.01 0.0199 0.0282 0.0091 0.0087
Golgi 0.0201 0.0044 0.0083 0.005 0.0026 0.0028 0.0125 0.0027 0.0118 0.034 0.0054 0.0181 0.0095 0.0012 0.0112 0.007 0.0017 0.004
Lipid Particles 0.0118 0.0005 0.0049 0.005 0.0007 0.0002 0.0144 0.0011 0.0019 0.0015 0.0009 0.0021 0.0041 0.0003 0.0038 0.0007 0.0006 0.0001
Mitochondria 0.0053 0.0008 0.0058 0.04 0.0006 0.0005 0.0041 0.0018 0.0092 0.0175 0.0009 0.0038 0.011 0.0006 0.0091 0.0012 0.0005 0.0007
None 0.0301 0.0367 0.0287 0.0152 0.0178 0.0163 0.0961 0.115 0.0596 0.0607 0.089 0.0266 0.1124 0.1148 0.0453 0.0302 0.0339 0.018
Nuclear Periphery 0.0139 0.0004 0.0028 0.0045 0.0005 0.0001 0.0271 0.001 0.0053 0.0017 0.0003 0.005 0.0084 0.0008 0.0005 0.001 0.0008 0.0001
Nucleolus 0.001 0.0001 0.0003 0.0015 0 0.0001 0.0012 0.0003 0.0006 0.0003 0.0001 0.0011 0.0004 0.0007 0.0003 0.0005 0.0001 0.0001
Nucleus 0.0063 0.0009 0.0019 0.0036 0.0006 0.0005 0.0197 0.003 0.003 0.0018 0.0005 0.0018 0.0019 0.002 0.0009 0.0023 0.0008 0.0004
Peroxisomes 0.0193 0.0003 0.0049 0.0023 0.0017 0.0008 0.0047 0.0007 0.0053 0.0042 0.002 0.0037 0.0049 0.0005 0.0062 0.0009 0.0033 0.0008
Punctate Nuclear 0.0241 0.0004 0.0016 0.0014 0.0029 0.0003 0.0068 0.0015 0.0015 0.0009 0.0003 0.0018 0.0082 0.0012 0.0009 0.0006 0.0015 0.0002
Vacuole 0.0028 0.002 0.0061 0.0157 0.0017 0.0009 0.004 0.0027 0.007 0.0126 0.0024 0.0016 0.0043 0.0027 0.0031 0.028 0.0033 0.0011
Vacuole Periphery 0.0013 0.0002 0.0011 0.0038 0.0002 0.0001 0.002 0.0003 0.002 0.0031 0.0004 0.0007 0.0014 0.0003 0.0008 0.0016 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.8085 11.0665 14.4788 23.6925 24.287 15.9542 19.8758 20.3707 20.5923 31.0373
Translational Efficiency 0.7533 1.1013 0.9036 0.5674 0.6955 1.3065 0.6419 0.6336 0.7012 0.6482

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Rho GTPase activating protein (RhoGAP); involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
Localization
Cell Percentages cytoplasm (66%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Bem3

Bem3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bem3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available