Standard name
Human Ortholog
Description Trichostatin A-insensitive homodimeric histone deacetylase (HDAC); specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0.05 0 0 0 0 – 0 0 0 0 0 0.05 0.11 0 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.1 0.07 0.06 0.05 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0.11 0.08 0 0 0 0 0 0
Cytoplasm 0.93 0.88 0.91 0.91 0.85 0.79 0.77 0.74 0.69 – 0.59 0.7 0.96 0.94 0.88 0.8 0.78 0.92 0.79 0.74 0.76 0.7 0.8 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0 0.05 0 0 0 0.08 0.1 0.07
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.16 0.17 0.13 0.37 – 0.39 0.24 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.09 0 – 0.08 0.07 0 0 0 0.08 0 0.08 0 0 0 0.07 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 9 24 14 26 12 17 9 8 – 3 10 3 7 12 4 5 1 7 11 5 1 3 2
Bud 5 15 11 11 23 25 28 8 22 – 20 26 8 3 12 2 2 0 6 19 17 3 1 7
Bud Neck 1 0 1 4 3 2 2 1 0 – 1 0 0 0 0 0 0 0 31 29 31 7 1 3
Bud Site 0 0 3 0 0 1 2 1 1 – 0 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 4 19 13 8 14 13 8 9 – 15 14 2 12 6 6 5 5 0 0 0 0 0 0
Cytoplasm 284 357 439 404 408 490 660 475 393 – 325 425 333 419 467 69 36 59 252 310 401 118 271 349
Endoplasmic Reticulum 5 9 5 3 7 12 21 4 9 – 4 5 7 10 6 5 2 3 4 5 4 13 33 30
Endosome 3 16 1 6 5 15 10 13 4 – 2 7 0 7 41 0 0 1 9 10 12 5 0 2
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 4 1 0 1 2 3 0 0 2
Mitochondria 10 9 8 9 22 99 143 84 209 – 216 144 8 1 2 4 3 0 0 1 19 3 9 13
Nucleus 0 3 1 0 4 2 11 8 8 – 5 13 1 1 0 0 0 1 0 1 1 0 0 0
Nuclear Periphery 2 0 0 1 1 2 9 0 1 – 1 2 0 0 0 0 0 0 2 1 2 1 0 0
Nucleolus 4 3 0 0 1 4 6 8 3 – 5 5 0 0 1 0 0 0 0 1 0 0 0 0
Peroxisomes 0 2 5 0 2 5 3 2 1 – 1 1 0 1 8 0 0 1 0 1 3 0 1 0
SpindlePole 0 0 6 2 6 8 3 18 3 – 5 9 0 1 2 1 0 0 1 5 10 0 0 1
Vac/Vac Membrane 4 11 2 10 8 20 46 60 25 – 45 42 0 4 2 7 1 5 1 15 7 11 15 22
Unique Cell Count 304 407 482 445 481 623 853 641 568 554 604 346 448 530 86 46 64 321 420 526 169 341 443
Labelled Cell Count 323 438 525 477 524 711 974 699 696 648 706 362 466 559 102 55 76 321 420 526 169 341 443


Bud Neck

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 6.8 6.4 5.9 6.5 5.7 5.8 6.2 5.3 – 4.9 5.8 6.1 6.8 7.0 13.6 13.1 13.0 6.0 6.3 6.7
Std Deviation (1e-4) 0.8 1.2 0.8 0.9 1.1 0.9 0.8 0.9 1.0 – 0.8 1.0 0.9 0.8 1.0 2.1 1.8 2.7 0.8 1.0 1.2
Intensity Change (Log2) – – – -0.13 0.01 -0.18 -0.15 -0.06 -0.28 – -0.41 -0.16 -0.07 0.07 0.12 1.08 1.02 1.01 -0.11 -0.04 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.4 0.3 -2.8 -3.0 -3.5 -3.4 – 0 -3.1 -3.3 -2.9 -2.3 0 0 0
Bud 0.2 2.1 1.6 1.0 -1.3 1.5 – 0 1.8 0 -2.0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery -0.9 -2.1 -1.6 -2.8 -2.9 -2.4 – 0 -1.5 -3.0 -1.1 -2.9 0 0 0
Cytoplasm -0.2 -3.0 -5.6 -6.3 -7.2 -8.7 – -11.8 -8.4 2.9 1.4 -1.5 -3.0 -2.8 0.3
Endoplasmic Reticulum 0 0.6 1.2 1.8 0 0.8 – 0 0 1.2 1.4 0.1 0 0 0
Endosome 0 0 3.0 0 2.7 0 – 0 0 0 0 6.0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.4 2.6 7.9 8.4 6.9 14.0 – 0 10.5 0.7 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 2.8 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0.7 0 0 0
SpindlePole 0 0 0.1 0 1.8 0 – 0 0.3 0 0 0 0 0 0
Vacuole 2.5 0 3.3 4.7 6.5 4.1 – 5.9 5.4 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1079 1.6477 1.4266 1.6763 1.3909 1.6134 1.7846 2.5472 2.4757 2.3875 2.3438 2.457 1.4927 2.3709 2.1946 2.3628 2.5399 2.1542
Actin 0.0154 0.0038 0.0042 0.0011 0.0609 0.007 0.0281 0.006 0.0268 0.0234 0.1093 0.0134 0.0331 0.0008 0.0157 0.0057 0.0481 0.013
Bud 0.0049 0.0066 0.002 0.0006 0.0034 0.0025 0.0017 0.0055 0.0021 0.0013 0.0042 0.0017 0.0016 0.0019 0.0027 0.0009 0.001 0.0009
Bud Neck 0.0111 0.0067 0.1246 0.6848 0.5924 0.1145 0.0088 0.0042 0.1263 0.5063 0.5428 0.1324 0.011 0.0064 0.1317 0.6057 0.7523 0.1259
Bud Periphery 0.0053 0.0053 0.0017 0.0006 0.0066 0.0032 0.0016 0.0053 0.0024 0.0013 0.0116 0.0042 0.0013 0.0007 0.0024 0.001 0.0018 0.0016
Bud Site 0.0437 0.1186 0.041 0.0034 0.0097 0.002 0.0305 0.055 0.0206 0.0051 0.0119 0.0042 0.066 0.0702 0.028 0.0045 0.0023 0.0012
Cell Periphery 0.0025 0.0013 0.001 0.0003 0.0011 0.0004 0.0008 0.0006 0.0009 0.0004 0.0011 0.0004 0.0009 0.0004 0.0009 0.0006 0.0003 0.0002
Cytoplasm 0.5699 0.6213 0.619 0.2175 0.1044 0.6696 0.6324 0.771 0.6403 0.3195 0.0051 0.7267 0.6246 0.828 0.7032 0.3238 0.0139 0.7392
Cytoplasmic Foci 0.0635 0.0406 0.0461 0.0389 0.0407 0.0385 0.0737 0.0468 0.0566 0.0596 0.038 0.0237 0.0729 0.0101 0.0346 0.0228 0.0194 0.0216
Eisosomes 0.0004 0.0001 0.0004 0 0.0002 0.0001 0.0003 0.0001 0.0002 0.0001 0.0005 0.0001 0.0004 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0157 0.0072 0.0054 0.0014 0.0074 0.0148 0.0211 0.0068 0.0106 0.0013 0.0022 0.0094 0.0216 0.0048 0.0058 0.002 0.0028 0.0169
Endosome 0.0509 0.0381 0.0224 0.0197 0.0235 0.0453 0.0549 0.0351 0.04 0.041 0.0632 0.0363 0.0364 0.01 0.0227 0.0086 0.0258 0.0282
Golgi 0.0064 0.0043 0.0044 0.0018 0.0111 0.0044 0.0194 0.004 0.01 0.0102 0.0658 0.0081 0.0113 0.0011 0.0054 0.0018 0.0079 0.0041
Lipid Particles 0.0192 0.0036 0.0007 0.0004 0.0079 0.0022 0.0209 0.0013 0.0072 0.0016 0.03 0.0024 0.0133 0.0002 0.0019 0.0003 0.0084 0.0047
Mitochondria 0.0047 0.0016 0.0105 0.0005 0.0011 0.0015 0.0172 0.0023 0.0055 0.0022 0.0423 0.0028 0.0267 0.0003 0.0052 0.0002 0.0041 0.0021
None 0.1092 0.1 0.105 0.0191 0.0249 0.0463 0.0401 0.0271 0.0201 0.0016 0.0025 0.0097 0.041 0.0474 0.0276 0.0108 0.0013 0.0208
Nuclear Periphery 0.03 0.0054 0.0008 0.0007 0.0574 0.0187 0.0086 0.0027 0.0031 0.0006 0.0283 0.0084 0.0049 0.001 0.0016 0.0004 0.0155 0.0047
Nucleolus 0.0012 0.0022 0.0005 0.0004 0.0038 0.0011 0.0012 0.0007 0.0008 0.0001 0.0018 0.0004 0.0008 0.0016 0.0003 0.0024 0.0048 0.0011
Nucleus 0.0156 0.0175 0.0021 0.0029 0.0061 0.0105 0.0032 0.0028 0.002 0.0004 0.0022 0.0025 0.0052 0.0056 0.0013 0.0029 0.006 0.0035
Peroxisomes 0.0051 0.0016 0.0012 0.0005 0.0044 0.0021 0.0169 0.0075 0.0102 0.0206 0.021 0.0035 0.0131 0.0001 0.0015 0.0002 0.0023 0.0017
Punctate Nuclear 0.0067 0.004 0.0013 0.0011 0.025 0.0045 0.0089 0.0023 0.0042 0.001 0.0095 0.0048 0.0074 0.0008 0.0022 0.0008 0.0782 0.0036
Vacuole 0.0144 0.0091 0.0049 0.0039 0.0064 0.0093 0.008 0.0113 0.0086 0.0017 0.0041 0.0038 0.0051 0.0079 0.0042 0.0041 0.0022 0.0037
Vacuole Periphery 0.0042 0.0011 0.0006 0.0004 0.0017 0.0017 0.0018 0.0016 0.0015 0.0006 0.0026 0.0012 0.0012 0.0006 0.001 0.0006 0.0015 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.4966 44.7496 9.088 12.8293 17.7631 17.3793 29.8864 28.288 19.0449 23.168
Translational Efficiency 0.8208 0.6034 0.7856 0.5039 0.7783 0.8076 0.7698 0.5623 0.6368 0.5799

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Trichostatin A-insensitive homodimeric histone deacetylase (HDAC); specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats
Localization
Cell Percentages cytoplasm (86%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Hos3

Hos3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hos3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available