Standard name | |
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Human Ortholog | |
Description | Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress; DBP1 has a paralog, DED1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 1 | 3 | – | 1 | 8 | 2 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 5 | 2 | 0 | 0 | 1 |
Bud | 4 | 1 | 0 | 0 | 1 | 1 | 4 | – | 2 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 12 | 3 | 0 | 2 | 0 |
Bud Neck | 0 | 0 | 2 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 8 | 6 | 2 | 6 | 6 | 4 | – | 6 | 19 | 4 | 5 | 1 | 6 | 6 | 40 | 40 | 29 | 0 | 1 | 1 | 0 | 0 | 0 |
Cytoplasm | 311 | 564 | 246 | 139 | 218 | 219 | 303 | – | 179 | 229 | 122 | 160 | 101 | 174 | 216 | 348 | 348 | 296 | 299 | 504 | 206 | 62 | 121 | 154 |
Endoplasmic Reticulum | 0 | 4 | 2 | 1 | 2 | 6 | 13 | – | 8 | 12 | 5 | 6 | 0 | 0 | 2 | 13 | 12 | 4 | 4 | 6 | 3 | 0 | 1 | 7 |
Endosome | 0 | 3 | 0 | 0 | 0 | 1 | 1 | – | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 5 | 1 | 0 | 2 | 2 |
Golgi | 0 | 0 | 1 | 0 | 2 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 3 |
Mitochondria | 5 | 3 | 0 | 2 | 6 | 27 | 42 | – | 79 | 72 | 146 | 93 | 0 | 1 | 1 | 4 | 2 | 0 | 12 | 18 | 32 | 2 | 1 | 3 |
Nucleus | 4 | 1 | 1 | 0 | 0 | 0 | 3 | – | 3 | 6 | 0 | 3 | 0 | 0 | 0 | 2 | 5 | 1 | 0 | 2 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | 3 | 0 | 0 | 2 | 3 | 9 | – | 24 | 15 | 6 | 13 | 0 | 1 | 1 | 23 | 13 | 14 | 2 | 10 | 4 | 0 | 1 | 1 |
Unique Cell Count | 322 | 575 | 248 | 141 | 229 | 242 | 357 | 263 | 317 | 244 | 252 | 101 | 176 | 218 | 379 | 379 | 315 | 335 | 586 | 261 | 71 | 135 | 179 | |
Labelled Cell Count | 330 | 587 | 258 | 145 | 240 | 264 | 382 | 304 | 367 | 288 | 287 | 102 | 182 | 226 | 433 | 421 | 344 | 335 | 586 | 261 | 71 | 135 | 179 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 5.6 | 5.8 | 5.6 | 6.7 | 6.0 | 6.6 | – | 6.4 | 6.5 | 5.7 | 6.1 | 6.8 | 6.7 | 6.5 | 20.3 | 20.7 | 19.9 | 5.1 | 6.4 | 6.7 |
Std Deviation (1e-4) | 0.8 | 1.2 | 0.9 | 1.5 | 1.4 | 1.1 | 1.4 | – | 1.5 | 1.6 | 1.9 | 1.7 | 1.6 | 1.0 | 1.2 | 5.2 | 4.9 | 4.8 | 0.9 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.22 | 0.07 | 0.19 | – | 0.15 | 0.18 | -0.01 | 0.08 | 0.24 | 0.21 | 0.18 | 1.82 | 1.85 | 1.78 | -0.19 | 0.14 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0.1 | 0 | 0 | – | -0.1 | 2.1 | 0 | -0.3 | 0 | 0 | 0.2 | 3.8 | 3.8 | 3.3 |
Cytoplasm | -0.6 | -2.7 | -4.4 | -6.0 | – | -9.4 | -8.7 | -12.6 | -10.2 | 0.9 | -0.3 | -0.1 | -4.0 | -4.0 | -3.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.2 | – | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 2.1 | 2.0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.4 | 5.6 | – | 9.4 | 8.0 | 14.5 | 10.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 4.9 | 3.5 | 0 | 3.6 | 0 | 0 | 0 | 4.0 | 2.9 | 3.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.6975 | 0.1122 | -0.009 | 0.1385 | 0.366 | -0.026 | 0.2461 | 0.6096 | 0.4905 | 0.6276 | 1.5597 | 0.6376 | 0.6652 | 0.6211 | 0.6491 | 0.2401 | 1.05 | 0.6823 |
Actin | 0.0881 | 0.001 | 0.0077 | 0.0249 | 0.0228 | 0.0143 | 0.0111 | 0.003 | 0.0135 | 0.0366 | 0.0338 | 0.0055 | 0.0223 | 0.0013 | 0.0095 | 0.0004 | 0.031 | 0.0075 |
Bud | 0.0044 | 0.0073 | 0.0027 | 0.0054 | 0.0034 | 0.005 | 0.0038 | 0.0071 | 0.002 | 0.0105 | 0.0017 | 0.0018 | 0.017 | 0.0185 | 0.0284 | 0.0067 | 0.027 | 0.0207 |
Bud Neck | 0.0049 | 0.0001 | 0.0008 | 0.0026 | 0.0032 | 0.0007 | 0.0051 | 0.0003 | 0.0026 | 0.0173 | 0.0031 | 0.0006 | 0.0055 | 0.0004 | 0.001 | 0.0005 | 0.0079 | 0.0064 |
Bud Periphery | 0.0094 | 0.0022 | 0.0007 | 0.0106 | 0.0045 | 0.0087 | 0.0112 | 0.0045 | 0.0035 | 0.0186 | 0.0032 | 0.007 | 0.0144 | 0.0097 | 0.0122 | 0.0023 | 0.0076 | 0.015 |
Bud Site | 0.0225 | 0.0117 | 0.0162 | 0.022 | 0.0294 | 0.0017 | 0.0114 | 0.0191 | 0.0116 | 0.0356 | 0.0055 | 0.0006 | 0.0296 | 0.0241 | 0.019 | 0.0011 | 0.0297 | 0.0053 |
Cell Periphery | 0.0013 | 0.0004 | 0.0006 | 0.0008 | 0.0013 | 0.0003 | 0.0006 | 0.0006 | 0.0005 | 0.0009 | 0.0004 | 0.0002 | 0.0011 | 0.0008 | 0.0012 | 0.0003 | 0.0011 | 0.0006 |
Cytoplasm | 0.1108 | 0.2446 | 0.2416 | 0.2162 | 0.2613 | 0.2491 | 0.1631 | 0.2085 | 0.1691 | 0.0887 | 0.1856 | 0.2181 | 0.273 | 0.4075 | 0.2751 | 0.4269 | 0.2057 | 0.4156 |
Cytoplasmic Foci | 0.0406 | 0.0074 | 0.0224 | 0.0177 | 0.0722 | 0.0318 | 0.0238 | 0.0115 | 0.0092 | 0.0201 | 0.049 | 0.0101 | 0.0159 | 0.0099 | 0.0168 | 0.0164 | 0.0407 | 0.0183 |
Eisosomes | 0.0012 | 0.0001 | 0.0004 | 0.0006 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0009 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0001 |
Endoplasmic Reticulum | 0.0105 | 0.0016 | 0.0033 | 0.0046 | 0.0036 | 0.0023 | 0.0026 | 0.0013 | 0.0021 | 0.0007 | 0.0049 | 0.0023 | 0.0044 | 0.003 | 0.0024 | 0.0023 | 0.0046 | 0.0018 |
Endosome | 0.0456 | 0.0079 | 0.0166 | 0.0202 | 0.0603 | 0.0197 | 0.0038 | 0.0031 | 0.0031 | 0.0071 | 0.0328 | 0.004 | 0.0083 | 0.0038 | 0.0066 | 0.0079 | 0.0412 | 0.0037 |
Golgi | 0.0141 | 0.0003 | 0.0016 | 0.0232 | 0.035 | 0.0087 | 0.001 | 0.0005 | 0.001 | 0.0052 | 0.0115 | 0.0008 | 0.002 | 0.0009 | 0.0009 | 0.0006 | 0.0095 | 0.0005 |
Lipid Particles | 0.0247 | 0.0005 | 0.0029 | 0.0237 | 0.0215 | 0.0053 | 0.0023 | 0.0005 | 0.002 | 0.005 | 0.0118 | 0.0014 | 0.001 | 0.0002 | 0.0011 | 0.0002 | 0.0105 | 0.0003 |
Mitochondria | 0.0207 | 0.0004 | 0.0139 | 0.0113 | 0.014 | 0.0085 | 0.0018 | 0.001 | 0.0047 | 0.0276 | 0.0082 | 0.003 | 0.0017 | 0.0026 | 0.0023 | 0.0007 | 0.006 | 0.002 |
None | 0.5395 | 0.7098 | 0.647 | 0.5922 | 0.4341 | 0.6268 | 0.7316 | 0.7285 | 0.7589 | 0.7028 | 0.5356 | 0.7311 | 0.5716 | 0.5076 | 0.5905 | 0.521 | 0.3887 | 0.4785 |
Nuclear Periphery | 0.024 | 0.0005 | 0.0015 | 0.0017 | 0.0075 | 0.0043 | 0.0074 | 0.0008 | 0.0019 | 0.0005 | 0.0409 | 0.0038 | 0.0063 | 0.0008 | 0.0016 | 0.0018 | 0.0115 | 0.0022 |
Nucleolus | 0.002 | 0.0002 | 0.0014 | 0.0003 | 0.0009 | 0.0004 | 0.0006 | 0.0004 | 0.0008 | 0.0007 | 0.0098 | 0.0003 | 0.0012 | 0.001 | 0.0026 | 0.0003 | 0.0064 | 0.0022 |
Nucleus | 0.0041 | 0.0009 | 0.0028 | 0.0011 | 0.0024 | 0.0015 | 0.0041 | 0.0022 | 0.005 | 0.0014 | 0.0271 | 0.0012 | 0.0066 | 0.0033 | 0.0106 | 0.0041 | 0.0375 | 0.0078 |
Peroxisomes | 0.0179 | 0.0001 | 0.0011 | 0.0151 | 0.0038 | 0.0041 | 0.0091 | 0.0041 | 0.0027 | 0.0164 | 0.0087 | 0.0045 | 0.0007 | 0.0003 | 0.0042 | 0.0005 | 0.0068 | 0.0005 |
Punctate Nuclear | 0.0048 | 0.0004 | 0.0094 | 0.0017 | 0.0071 | 0.0025 | 0.0035 | 0.0013 | 0.0035 | 0.0015 | 0.0186 | 0.0023 | 0.0141 | 0.0018 | 0.0101 | 0.002 | 0.1207 | 0.0088 |
Vacuole | 0.0057 | 0.0023 | 0.0047 | 0.0033 | 0.009 | 0.0028 | 0.0014 | 0.0014 | 0.0016 | 0.0022 | 0.0042 | 0.0009 | 0.0024 | 0.002 | 0.0032 | 0.0034 | 0.0039 | 0.0019 |
Vacuole Periphery | 0.0033 | 0.0002 | 0.0007 | 0.0007 | 0.0028 | 0.0012 | 0.0005 | 0.0002 | 0.0003 | 0.0005 | 0.0026 | 0.0003 | 0.0006 | 0.0003 | 0.0006 | 0.0006 | 0.0017 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 4.166 | 1.5961 | 4.3426 | 3.4487 | 4.244 | 4.7585 | 2.6897 | 5.9906 | 6.0646 | 4.8727 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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