Standard name
Human Ortholog
Description Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress; DBP1 has a paralog, DED1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.11 0.11 0.09 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.99 0.99 0.95 0.9 0.85 0.68 0.72 0.5 0.63 1.0 0.99 0.99 0.92 0.92 0.94 0.89 0.86 0.79 0.88 0.9 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.12 0.3 0.23 0.6 0.37 0 0 0 0 0 0 0 0 0.12 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.09 0.05 0 0.05 0 0 0 0.06 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 3 1 8 2 3 0 0 0 1 0 0 1 5 2 0 0 1
Bud 4 1 0 0 1 1 4 2 0 3 2 0 0 0 0 0 0 2 12 3 0 2 0
Bud Neck 0 0 2 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 4 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 4 8 6 2 6 6 4 6 19 4 5 1 6 6 40 40 29 0 1 1 0 0 0
Cytoplasm 311 564 246 139 218 219 303 179 229 122 160 101 174 216 348 348 296 299 504 206 62 121 154
Endoplasmic Reticulum 0 4 2 1 2 6 13 8 12 5 6 0 0 2 13 12 4 4 6 3 0 1 7
Endosome 0 3 0 0 0 1 1 2 2 0 0 0 0 0 1 0 0 3 5 1 0 2 2
Golgi 0 0 1 0 2 0 0 0 1 0 1 0 0 0 1 1 0 0 3 0 0 0 3
Mitochondria 5 3 0 2 6 27 42 79 72 146 93 0 1 1 4 2 0 12 18 32 2 1 3
Nucleus 4 1 1 0 0 0 3 3 6 0 3 0 0 0 2 5 1 0 2 1 0 0 0
Nuclear Periphery 2 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 1 0
Vac/Vac Membrane 0 3 0 0 2 3 9 24 15 6 13 0 1 1 23 13 14 2 10 4 0 1 1
Unique Cell Count 322 575 248 141 229 242 357 263 317 244 252 101 176 218 379 379 315 335 586 261 71 135 179
Labelled Cell Count 330 587 258 145 240 264 382 304 367 288 287 102 182 226 433 421 344 335 586 261 71 135 179


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.6 5.8 5.6 6.7 6.0 6.6 6.4 6.5 5.7 6.1 6.8 6.7 6.5 20.3 20.7 19.9 5.1 6.4 6.7
Std Deviation (1e-4) 0.8 1.2 0.9 1.5 1.4 1.1 1.4 1.5 1.6 1.9 1.7 1.6 1.0 1.2 5.2 4.9 4.8 0.9 1.4 1.5
Intensity Change (Log2) -0.05 0.22 0.07 0.19 0.15 0.18 -0.01 0.08 0.24 0.21 0.18 1.82 1.85 1.78 -0.19 0.14 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.1 0 0 -0.1 2.1 0 -0.3 0 0 0.2 3.8 3.8 3.3
Cytoplasm -0.6 -2.7 -4.4 -6.0 -9.4 -8.7 -12.6 -10.2 0.9 -0.3 -0.1 -4.0 -4.0 -3.2
Endoplasmic Reticulum 0 0 0 2.2 0 2.3 0 0 0 0 0 2.1 2.0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.4 5.6 9.4 8.0 14.5 10.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 4.9 3.5 0 3.6 0 0 0 4.0 2.9 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6975 0.1122 -0.009 0.1385 0.366 -0.026 0.2461 0.6096 0.4905 0.6276 1.5597 0.6376 0.6652 0.6211 0.6491 0.2401 1.05 0.6823
Actin 0.0881 0.001 0.0077 0.0249 0.0228 0.0143 0.0111 0.003 0.0135 0.0366 0.0338 0.0055 0.0223 0.0013 0.0095 0.0004 0.031 0.0075
Bud 0.0044 0.0073 0.0027 0.0054 0.0034 0.005 0.0038 0.0071 0.002 0.0105 0.0017 0.0018 0.017 0.0185 0.0284 0.0067 0.027 0.0207
Bud Neck 0.0049 0.0001 0.0008 0.0026 0.0032 0.0007 0.0051 0.0003 0.0026 0.0173 0.0031 0.0006 0.0055 0.0004 0.001 0.0005 0.0079 0.0064
Bud Periphery 0.0094 0.0022 0.0007 0.0106 0.0045 0.0087 0.0112 0.0045 0.0035 0.0186 0.0032 0.007 0.0144 0.0097 0.0122 0.0023 0.0076 0.015
Bud Site 0.0225 0.0117 0.0162 0.022 0.0294 0.0017 0.0114 0.0191 0.0116 0.0356 0.0055 0.0006 0.0296 0.0241 0.019 0.0011 0.0297 0.0053
Cell Periphery 0.0013 0.0004 0.0006 0.0008 0.0013 0.0003 0.0006 0.0006 0.0005 0.0009 0.0004 0.0002 0.0011 0.0008 0.0012 0.0003 0.0011 0.0006
Cytoplasm 0.1108 0.2446 0.2416 0.2162 0.2613 0.2491 0.1631 0.2085 0.1691 0.0887 0.1856 0.2181 0.273 0.4075 0.2751 0.4269 0.2057 0.4156
Cytoplasmic Foci 0.0406 0.0074 0.0224 0.0177 0.0722 0.0318 0.0238 0.0115 0.0092 0.0201 0.049 0.0101 0.0159 0.0099 0.0168 0.0164 0.0407 0.0183
Eisosomes 0.0012 0.0001 0.0004 0.0006 0.0002 0.0001 0.0004 0.0002 0.0002 0.0003 0.0009 0.0001 0.0003 0.0001 0.0002 0.0001 0.0005 0.0001
Endoplasmic Reticulum 0.0105 0.0016 0.0033 0.0046 0.0036 0.0023 0.0026 0.0013 0.0021 0.0007 0.0049 0.0023 0.0044 0.003 0.0024 0.0023 0.0046 0.0018
Endosome 0.0456 0.0079 0.0166 0.0202 0.0603 0.0197 0.0038 0.0031 0.0031 0.0071 0.0328 0.004 0.0083 0.0038 0.0066 0.0079 0.0412 0.0037
Golgi 0.0141 0.0003 0.0016 0.0232 0.035 0.0087 0.001 0.0005 0.001 0.0052 0.0115 0.0008 0.002 0.0009 0.0009 0.0006 0.0095 0.0005
Lipid Particles 0.0247 0.0005 0.0029 0.0237 0.0215 0.0053 0.0023 0.0005 0.002 0.005 0.0118 0.0014 0.001 0.0002 0.0011 0.0002 0.0105 0.0003
Mitochondria 0.0207 0.0004 0.0139 0.0113 0.014 0.0085 0.0018 0.001 0.0047 0.0276 0.0082 0.003 0.0017 0.0026 0.0023 0.0007 0.006 0.002
None 0.5395 0.7098 0.647 0.5922 0.4341 0.6268 0.7316 0.7285 0.7589 0.7028 0.5356 0.7311 0.5716 0.5076 0.5905 0.521 0.3887 0.4785
Nuclear Periphery 0.024 0.0005 0.0015 0.0017 0.0075 0.0043 0.0074 0.0008 0.0019 0.0005 0.0409 0.0038 0.0063 0.0008 0.0016 0.0018 0.0115 0.0022
Nucleolus 0.002 0.0002 0.0014 0.0003 0.0009 0.0004 0.0006 0.0004 0.0008 0.0007 0.0098 0.0003 0.0012 0.001 0.0026 0.0003 0.0064 0.0022
Nucleus 0.0041 0.0009 0.0028 0.0011 0.0024 0.0015 0.0041 0.0022 0.005 0.0014 0.0271 0.0012 0.0066 0.0033 0.0106 0.0041 0.0375 0.0078
Peroxisomes 0.0179 0.0001 0.0011 0.0151 0.0038 0.0041 0.0091 0.0041 0.0027 0.0164 0.0087 0.0045 0.0007 0.0003 0.0042 0.0005 0.0068 0.0005
Punctate Nuclear 0.0048 0.0004 0.0094 0.0017 0.0071 0.0025 0.0035 0.0013 0.0035 0.0015 0.0186 0.0023 0.0141 0.0018 0.0101 0.002 0.1207 0.0088
Vacuole 0.0057 0.0023 0.0047 0.0033 0.009 0.0028 0.0014 0.0014 0.0016 0.0022 0.0042 0.0009 0.0024 0.002 0.0032 0.0034 0.0039 0.0019
Vacuole Periphery 0.0033 0.0002 0.0007 0.0007 0.0028 0.0012 0.0005 0.0002 0.0003 0.0005 0.0026 0.0003 0.0006 0.0003 0.0006 0.0006 0.0017 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.166 1.5961 4.3426 3.4487 4.244 4.7585 2.6897 5.9906 6.0646 4.8727
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress; DBP1 has a paralog, DED1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (45%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dbp1

Dbp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dbp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available