Standard name | |
---|---|
Human Ortholog | |
Description | Karyopherin responsible for the nuclear import of Rpf1p; Rpf1p is a ribosome maturation factor |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 1 | 0 | 1 | 0 | 1 | 1 | 2 | 2 | – | 7 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 11 | 14 | 13 | 18 | 17 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | – | 6 | 6 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 10 | 25 | 54 | 48 | 37 | 53 | 48 | 60 | – | 57 | 43 | 31 | 38 | 74 | 106 | 12 | 23 | 9 | 9 | 14 | 26 | 20 | 24 | 20 |
Endoplasmic Reticulum | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3 | 6 | 4 | 0 | 2 | 2 | 0 | 0 | 3 | 2 | 3 | 4 |
Endosome | 1 | 0 | 2 | 5 | 15 | 3 | 3 | 7 | – | 0 | 2 | 4 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 3 | 6 | 4 | 8 | 11 |
Golgi | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 |
Mitochondria | 0 | 9 | 5 | 6 | 11 | 37 | 49 | 104 | – | 211 | 154 | 165 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 7 | 2 | 0 | 2 | 4 |
Nucleus | 317 | 116 | 229 | 151 | 176 | 203 | 257 | 338 | – | 286 | 248 | 245 | 91 | 109 | 82 | 25 | 32 | 15 | 426 | 206 | 355 | 124 | 195 | 161 |
Nuclear Periphery | 0 | 0 | 1 | 1 | 3 | 6 | 2 | 6 | – | 12 | 4 | 13 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 15 | 2 | 6 | 5 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 34 | – | 12 | 9 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 2 | 8 | 1 | 2 | 5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 5 | 3 | 4 | 4 |
Vac/Vac Membrane | 232 | 147 | 264 | 219 | 266 | 176 | 208 | 235 | – | 151 | 77 | 133 | 123 | 157 | 206 | 12 | 19 | 23 | 28 | 21 | 72 | 39 | 99 | 124 |
Unique Cell Count | 461 | 239 | 486 | 376 | 453 | 421 | 515 | 691 | 604 | 438 | 471 | 215 | 274 | 332 | 43 | 64 | 40 | 494 | 274 | 525 | 218 | 372 | 369 | |
Labelled Cell Count | 562 | 297 | 559 | 432 | 511 | 479 | 576 | 789 | 742 | 549 | 611 | 255 | 346 | 399 | 50 | 77 | 50 | 494 | 274 | 525 | 218 | 372 | 369 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.1 | 8.1 | 8.1 | 8.1 | 7.5 | 6.8 | 6.8 | 6.6 | – | 5.8 | 5.9 | 6.1 | 9.6 | 8.4 | 9.7 | 7.2 | 7.8 | 10.5 | 9.9 | 10.4 | 11.0 |
Std Deviation (1e-4) | 1.3 | 1.6 | 1.4 | 1.6 | 1.6 | 1.5 | 1.8 | 1.5 | – | 1.4 | 1.6 | 1.4 | 1.4 | 1.1 | 1.4 | 1.7 | 1.9 | 3.1 | 1.4 | 1.5 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.0 | -0.12 | -0.26 | -0.26 | -0.29 | – | -0.49 | -0.47 | -0.42 | 0.24 | 0.05 | 0.25 | -0.17 | -0.06 | 0.37 | 0.28 | 0.35 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7 | -1.5 | 0.7 | -0.9 | -1.4 | – | -0.9 | -0.6 | -2.5 | 2.4 | 5.6 | 7.4 | 3.2 | 5.4 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 3.3 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1.7 | 5.5 | 5.9 | 8.2 | – | 14.0 | 13.7 | 13.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -2.0 | -2.6 | 0.3 | 0.9 | 0.6 | – | 0.1 | 2.9 | 1.5 | -1.2 | -2.0 | -6.5 | 1.4 | 0.4 | -1.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 2.7 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 5.0 | – | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.2 | 1.4 | -3.8 | -4.4 | -6.9 | – | -9.9 | -11.6 | -8.2 | 0.7 | 0.8 | 2.2 | -3.3 | -3.7 | 0.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.6923 | 5.9137 | 5.3435 | 5.0129 | 4.1496 | 5.1886 | 5.6813 | 7.5416 | 6.6189 | 5.858 | 6.4514 | 6.4277 | 5.5052 | 7.6961 | 6.1024 | 5.8783 | 5.7368 | 6.0234 |
Actin | 0.0248 | 0.0001 | 0.0037 | 0 | 0.0003 | 0.0002 | 0.0263 | 0.0034 | 0.0061 | 0.005 | 0.002 | 0.0087 | 0.0063 | 0.0006 | 0.0006 | 0.0001 | 0.0083 | 0.009 |
Bud | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0006 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0001 |
Bud Neck | 0.001 | 0.0001 | 0.0003 | 0.0003 | 0.0005 | 0.0011 | 0.0016 | 0.0015 | 0.0009 | 0.0009 | 0.0002 | 0.0014 | 0.0003 | 0.0006 | 0.0004 | 0.0003 | 0.0019 | 0.0014 |
Bud Periphery | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0012 | 0.0004 | 0.0003 | 0.0011 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0008 | 0.0003 |
Bud Site | 0.0006 | 0.0001 | 0.0009 | 0 | 0.0002 | 0 | 0.0018 | 0.0176 | 0.0011 | 0.0007 | 0.0008 | 0.0001 | 0.0006 | 0.0027 | 0.0006 | 0.0001 | 0.0006 | 0.0002 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0.0006 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0004 | 0.0001 |
Cytoplasm | 0.0055 | 0.0182 | 0.0065 | 0.0033 | 0.0216 | 0.0078 | 0.0198 | 0.0038 | 0.0147 | 0.0144 | 0.0011 | 0.0051 | 0.0569 | 0.0187 | 0.0313 | 0.0102 | 0.0208 | 0.0063 |
Cytoplasmic Foci | 0.0093 | 0.0037 | 0.0032 | 0 | 0.0006 | 0.0005 | 0.0197 | 0.0017 | 0.0135 | 0.0026 | 0.0068 | 0.001 | 0.0243 | 0.0008 | 0.0035 | 0 | 0.0066 | 0.002 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.002 | 0.0005 | 0.0009 | 0.0008 | 0.0034 | 0.0008 | 0.0094 | 0.0006 | 0.0034 | 0.0009 | 0.0001 | 0.0019 | 0.002 | 0.0006 | 0.0037 | 0.0003 | 0.0019 | 0.0011 |
Endosome | 0.0117 | 0.005 | 0.0017 | 0.0003 | 0.0023 | 0.0017 | 0.0274 | 0.0033 | 0.0115 | 0.0085 | 0.0026 | 0.0023 | 0.0131 | 0.0008 | 0.011 | 0.0001 | 0.0173 | 0.0131 |
Golgi | 0.0045 | 0.0004 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0065 | 0.0016 | 0.0049 | 0.005 | 0.0017 | 0.0013 | 0.0043 | 0.0007 | 0.0021 | 0 | 0.0049 | 0.0094 |
Lipid Particles | 0.0176 | 0.0013 | 0.0014 | 0 | 0.0001 | 0.0007 | 0.0174 | 0.0087 | 0.0077 | 0.0061 | 0.0044 | 0.0024 | 0.0262 | 0.0021 | 0.0012 | 0 | 0.0034 | 0.0047 |
Mitochondria | 0.0018 | 0.0002 | 0.0013 | 0.0001 | 0.0007 | 0.0002 | 0.0107 | 0.0053 | 0.0096 | 0.0497 | 0.0003 | 0.0008 | 0.0205 | 0.009 | 0.0021 | 0.0002 | 0.0064 | 0.0214 |
None | 0.0019 | 0.0001 | 0.0005 | 0 | 0.0022 | 0.0003 | 0.0116 | 0.01 | 0.0034 | 0.0003 | 0.0005 | 0.0004 | 0.007 | 0.0001 | 0.0009 | 0.0001 | 0.0025 | 0.0002 |
Nuclear Periphery | 0.0298 | 0.0186 | 0.0364 | 0.0296 | 0.0346 | 0.0171 | 0.0795 | 0.0187 | 0.0356 | 0.0271 | 0.0042 | 0.0196 | 0.032 | 0.0345 | 0.0412 | 0.0204 | 0.0192 | 0.0173 |
Nucleolus | 0.0018 | 0.0008 | 0.0009 | 0.0007 | 0.0032 | 0.0018 | 0.0035 | 0.0019 | 0.0006 | 0.0009 | 0.003 | 0.0015 | 0.0011 | 0.0009 | 0.0012 | 0.0006 | 0.0021 | 0.0015 |
Nucleus | 0.864 | 0.9495 | 0.9363 | 0.9639 | 0.924 | 0.9621 | 0.7214 | 0.9107 | 0.8767 | 0.8666 | 0.924 | 0.9479 | 0.7746 | 0.9207 | 0.8927 | 0.9663 | 0.883 | 0.9059 |
Peroxisomes | 0.0066 | 0.0002 | 0.003 | 0 | 0 | 0.0001 | 0.0076 | 0.0061 | 0.003 | 0.0056 | 0.0035 | 0.0019 | 0.0185 | 0.0048 | 0.0004 | 0 | 0.0036 | 0.0008 |
Punctate Nuclear | 0.015 | 0.0006 | 0.0012 | 0.0003 | 0.0039 | 0.0046 | 0.0271 | 0.0023 | 0.0056 | 0.0017 | 0.044 | 0.0014 | 0.0097 | 0.0009 | 0.003 | 0.001 | 0.0071 | 0.0021 |
Vacuole | 0.0008 | 0.0004 | 0.0005 | 0.0003 | 0.0016 | 0.0005 | 0.0042 | 0.0012 | 0.0008 | 0.0013 | 0.0001 | 0.0013 | 0.0014 | 0.0008 | 0.0024 | 0.0002 | 0.0075 | 0.0016 |
Vacuole Periphery | 0.0006 | 0.0002 | 0.0005 | 0.0002 | 0.0005 | 0.0002 | 0.0022 | 0.0003 | 0.0004 | 0.0009 | 0.0001 | 0.0004 | 0.0012 | 0.0003 | 0.0009 | 0.0001 | 0.0015 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.3743 | 15.5961 | 16.5329 | 28.7413 | 20.312 | 17.7927 | 23.6539 | 27.2596 | 24.2068 | 32.0855 |
Translational Efficiency | 0.6144 | 0.9808 | 1.0207 | 0.5613 | 0.7406 | 1.0972 | 0.737 | 0.5545 | 0.6418 | 0.6064 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|